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Comparison of Endonuclease-Sensitive Sites by T4 Endonuclease V and UvrABC Nuclease Treatments Followed by Formamide or Sodium Hydroxide Denaturation

  • Chang, Yung-Jin
    • BMB Reports
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    • v.31 no.4
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    • pp.405-408
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    • 1998
  • Endonuclease-sensitive sites detected by T4 endonuclease V or UvrABC nuclease treatments were compared in the dihydrofolate reductase gene of UV-irradiated Chinese hamster ovary B-11 cells. The number of endonuclease-sensitive sites detected by T4 endonuclease V treatment followed by NaOH denaturation was twice that of formamide denaturation. Repeated treatment of damaged genomic DNA with T4 endonuclease V resulted in no further increase in the number of endonuclease-sensitive sites detected. The numbers of endonuclease-sensitive sites detected by UvrABC nuclease using each denaturation condition were similar. Sequential treatment with the two endonucleases using formamide denaturation resulted in twice the number of endonuclease-sensitive sites detected by treatment of each nuclease alone. Due to a lack of AP endonuclease activity these results suggest the presence of T4 endonuclease V-sensitive sites which could be complemented by alkaline gel separation or by UvrABC nuclease treatment.

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Investigation on Excessive Vibration Signals of Two-Pole Generator Rotors in Balancing (발전기 양극 회전자 밸런싱에서의 이상 진동신호 분석)

  • 박종포;최성필;주영호
    • Journal of KSNVE
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    • v.9 no.4
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    • pp.835-840
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    • 1999
  • Cause of excessive vibration signals with twice the rotational speed of a 2-pole generator rotor in balancing for fossil power plants was investigated. The 2-pole generator rotor is treated as a typically asymmetric rotor in vibration analysis, and produces asynchronous vibration with twice the rotational speed for its own inertia and stiffness asymmetry. This paper introduces practical balancing procedure and experimental vibration data of the asymmetric 2-pole rotor in balancing, and presents the results of investigation into sources of the excessive vibration signals.

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Mutagenicity of 2-[(4- Cyanophenyl)amino] -3-chloro-1, 4- naphthalenedione (NQ-Y15) (2-[(4- Cyanophenyl)amino] -3-chloro-1, 4- naphthalenedione (NQ-Y15)의 돌연 변이원성)

  • 김봉희;정기화;유충규;창동신;이기선;전선덕;소동수;채상호;문창규
    • Environmental Analysis Health and Toxicology
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    • v.15 no.4
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    • pp.157-163
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    • 2000
  • 2- [(4- Cyanophenyl)amino] -3-chloro-1, 4- naphthalenedione (NQ-Y15) was asssayed for its genotoxic potential by using Salmonella typhimurium reversion assay and in vitro chromosome aberration test on Chinese hamster lung cells. In the Ames test, NQ-Y15 induced his + revertants of Salmonella typhimurium TA 98 and TA1537, reaching levels twice the negative control values. But, NQ-Y15 induced only his+ revertants of Salmonella typhimurium TA1537 more than twice the control values under the condition with metabolic activation system. In the cytogenetic test on chinese hamster lung cells. NQ -Y15 showed significant chromosomal aberrations, but the incidence was significantly reduced in the presence of metabolic activation.

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Influences of Sosiho-Tang Extracts and Prednisolone on the Toxicity of Carbon tetrachloride in Rats (랏트에 있어서 사염화탄소 독성에 대한 소시호탕 엑기스와 prednisolone의 영향)

  • 안영근;김성오;정대영
    • Environmental Analysis Health and Toxicology
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    • v.4 no.3_4
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    • pp.61-75
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    • 1989
  • Influences of the concurrent administration of carbon tetrachloride and prednisolone on the biometric, biochemical and histological findings were investigated in male rats prefered twice Sosio-Tang extracts at intervals of 24 hours. Influences of the concurrent admininistration of carbon tetrachlorides and prednisolone twice a week respectively on the findings were also investigated in male rats fed freely diet and. tab water mixed with Sosio-Tang extracts for six weeks. 1. Sosio-Tang extracts. decreased the toxicity of carbon tetrachloride. This was proved by biometric, biochemical and histological findings. 2. Prednisolone increased the toxicity caused by carbon tetrachloride. 3. The group treated with Sosio-Tang extracts. and prednisolon concomitantly increased the toxicity compared with Sosio-Tang extracts. treated group.

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Characteristics of LPB Having Gold Anode Fabricated by Sputtering Methode. (스퍼트링법에 의해 부극을 금으로 코팅시킨 LPB의 특성 연구)

  • 정재국;남효덕
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2001.07a
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    • pp.1043-1046
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    • 2001
  • In this study, characteristics of LPB having gold anode fabricated by sputtering methode were analyzed. As results, The 1st efficiency and the impedance characteristics of LPB decreased with increased gold coated on anode and current collector. But the rate characteristics and charge-discharge cycling characteristics increased with increased gold coated on anode and current collector. During 2C discharge of the rate characteristics test, the rate characteristics of LPB without gold coated and the rate characteristics of LPB with gold coated in twice were 159mAh/g and 189mAh/g, respectively. The discharge capacity was gradually degreased with the discharge cycling to about 20th cycles. But LPB with gold coated in twice was stabilized than LPB without gold coated.

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Seasonal Flank Alopecia in a Dog (개에서 발생한 계절성 옆구리 탈모증 1례)

  • Park, Seong-Jun
    • Journal of Veterinary Clinics
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    • v.31 no.4
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    • pp.341-343
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    • 2014
  • A 6-year-old female, shih-tzu was presented in the February with bilateral, symmetrical hair loss and hyperpigmentation on caudal dorsolumbar region. Skin test and blood examination found no remarkable finding. Histopathological examination of biopsies from the alopecic lesions revealed follicular atrophy, infundibular hyperkeratosis and some follicles were twisted truncated and dilated. The diagnosis of recurrent flank alopecia was based on clinical signs and supportive histopathology. Treatment with oral melatonin was started at 3 mg twice daily for 80 days, and by this time complete hair regrowth was observed. The following year in the September, a small non-inflammatory alopecia developed in the same site. The dog was given oral melatonin at 3 mg twice daily for 4 weeks, hair had completely regrown.

Electrochemical spike oscillation st the Ni electrode interface (Ni 전극 계면에서 전기화학적 spike 발진)

  • 천장호;손광철;라극환
    • Journal of the Korean Institute of Telematics and Electronics A
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    • v.33A no.12
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    • pp.83-89
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    • 1996
  • The electrochemical spike oscillations at the nickel (Ni) electrode/(0.05M KHC$_{8}$H$_{4}$O$_{4}$) buffer solution (pH 9) interface have been studied using voltammetric and chronoamperometric methods. The nature of the periodic cathodic current spikes is the activation controlled currents due to the hydrogen evolution reaction and depends onthe fractioanl surface coverage of the adsorbed hydrogen intermediate or the cathodic potential. There is two kinds of the waveforms corresponding to two kinds of the cathodic current spike oscillations. The widths, periods, and amplitudes of the cathodic current spikes are 4 ms or 5ms, 151 ms or 302 ms, and < 30 mA or < 275 mA, respectively. The fast discharge and recombination reaction steps are 1.5 times and twice and faster than the slow discharge and recombination reaction steps. The fast and slow discharge and recombination reaction steps are 1.5 times and twice faster than the slow discharge and recombination reaction steps. The fast and slow discharge and recombination reactions corresponding to the fast and slow adsorption sites at the Ni cathode.

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A High Speed and Area Efficient FFT Algorithm and Its Hardware Implementation (고속 및 면적 효율적인 FFT 알고리즘 개발 및 하드웨어 구현)

  • 탁연지;정윤호;김재석;박현철;김동규;박준현;유봉위
    • Proceedings of the IEEK Conference
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    • 2000.11b
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    • pp.297-300
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    • 2000
  • This paper proposes a high-speed and area-efficient FFT algorithm and performs a hardware implementation. This algorithm, named by “Radix-4/2”, uses the feature of existing radix-2$^3$algorithm, It reduces the number of non-trivial multipliers in SFG to the ratio of 3 to 2 campared with radix-2 or radix-4 algorithm and radix-4/2 has also twice throughput as radix-2$^3$algorithm's. It is proved that FFT processor using the proposed algorithm and 64-point MDC pipeline architecture has twice throughput as radix-2$^3$algorithm's, and reduces areas by 25 percentages in contrast to radix-4 algorithm's.

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A Study on Rhythmic Units in Korean -with Respect to Syntactic Structure- (한국어의 리듬 단위에 관한 연구 - 문법 구조와 관련하여)

  • Kim, Sun-Mi
    • Proceedings of the KSPS conference
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    • 1996.10a
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    • pp.224-228
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    • 1996
  • This paper is intended as a study on how an utterance is divided into rhythmic units in Standard Korean with respect to its syntactic structure. With respect to the data in this study I used 150 sentences which contained similar number of words and various syntactic structures. Those sentences were read by 7 speakers of Seoul dialect in a conversation style. Each sentence was read twice in a normal speed and twice in a fast speed. As a total, 4200 sentences were recorded. Then listening to them, the author marked the sentences with two kinds of boundaries i.e. strong and weak. To explore the relationship between rhythmic units and syntactic structure I devised a framework of grammatical symbols. Each symbol is designed to have both syntactic and morphological information at the same time. So I assigned those grammatical symbols to the sentences. Having sentences marked with grammatical symbols on the one hand, and with the rhythmic boundaries on the other hand, 1 could show the relationship between rhythmic units and syntactic structure; which syntactic structures are likely to be pronounced as one rhythmic unit, and which are on the rhythmic boundaries.

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Development of a New Design Theory for Incrementally Prestressed Concrete Girder (프리스트레스를 단계적으로 도입하는 IPC 거더의 설계 이론 연구)

  • 한만엽;김진근;이차돈;박준범
    • Journal of the Korea Concrete Institute
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    • v.12 no.4
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    • pp.121-130
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    • 2000
  • Current engineering practice in determining sectional dimensions of prestressed concrete (PSC) girders for bridges is primarily based on the code-specified allowable concrete stresses at different loading stages. It is customary that tendons and sectional dimensions are calibrated and tendon forces are applied at once at the initial stage to keep the subsequent stresses occurring at different loading stages within the allowable stresses. This traditional tensioning method, however, usually results in a too conservative sectional depth in view of ultimate capacity of a girder. A new design method which can realize the reduction of sectional depth of PSC girders is theoretically suggested in this study. Tendons are tensioned twice at different loading stages: the initial stage and the stage after fresh slab concrete is cast. It can be shown that according to this technique, sectional depth can be significantly reduced and larger span can be realized compared to traditional ones. Parametric studies are performed with due considerations given to its practical applications.