• Title/Summary/Keyword: TR

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Improvement of Drought Tolerance in Transgenic Tobacco Plant (형질전환 담배의 내건성 개선)

  • Park, Yong Mok
    • Journal of Environmental Science International
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    • v.25 no.1
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    • pp.173-179
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    • 2016
  • Leaf water and osmotic potential, chlorophyll content, photosynthetic rate, and electrolyte leakage were measured to evaluate tolerance to water stress in wild-type (WT) and transgenic tobacco plants (TR) expressing copper/zink superoxide dismutase (CuZnSOD) and ascorbate peroxidase (APX) in chloroplasts. Leaf water potential of both WT and TR plants decreased similarly under water stress condition. However, leaf osmotic potential of TR plants more negatively decreased in the process of dehydration, compared with WT plants, suggesting osmotic adjustment. Stomatal conductance (Gs) in WT plants markedly decreased from the Day 4 after withholding water, while that in TR plants retained relatively high values. Relatively low chlorophyll content and photosynthetic rate under water stress were shown in WT plants since $4^{th}$ day after treatment. In particular, damage indicated by electrolyte leakage during water stress was higher in WT plants than in TR plants. On the other hand, SOD and APX activity was remarkably higher in TR plants. These results indicate that transgenic tobacco plants expressing copper/zink superoxide dismutase (CuZnSOD) and ascorbate peroxidase (APX) in chloroplasts improve tolerance to water stress.

Analysis of Handling Qualities for Smart Unmanned Aerial Vehicle in Helicopter Flight Mode (스마트 무인기의 회전익 모드 비행성 분석)

  • Lee, Jang-Ho;Kim, Eung-Tai
    • Journal of Advanced Navigation Technology
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    • v.9 no.2
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    • pp.185-192
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    • 2005
  • The aim of this paper is to analyze handling qualities of tiltrotor aircraft(TR-S4) in helicopter flight mode including hovering and forward flight. Analysis of handling qualities is composed of aircraft response to control inputs that effect on stability and controllability. In short term response analysis, bandwidth is the critical parameter for small amplitude motions since it relates to the ability of a pilot to crisply start and stop maneuver. The handling qualities of TR-S4 in helicopter mode are analyzed with a SAS and an attitude controller and are satisfied level 1 in almost criteria with simulation of TR-S4 6-DOF nonlinear model.

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Identification of P-Glycoprotein and Transport Mechanism of Paclitaxel in Syncytiotrophoblast Cells

  • Lee, Na-Young;Lee, Ha-Eun;Kang, Young-Sook
    • Biomolecules & Therapeutics
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    • v.22 no.1
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    • pp.68-72
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    • 2014
  • When chemotherapy is administered during pregnancy, it is important to consider the fetus chemotherapy exposure, because it may lead to fetal consequences. Paclitaxel has become widely used in the metastatic and adjuvant settings for woman with cancer including breast and ovarian cancer. Therefore, we attempted to clarify the transport mechanisms of paclitaxel through blood-placenta barrier using rat conditionally immortalized syncytiotrophoblast cell lines (TR-TBTs). The uptake of paclitaxel was time- and temperature-dependent. Paclitaxel was eliminated about 50% from the cells within 30 min. The uptake of paclitaxel was saturable with $K_m$ of $168{\mu}M$ and $371{\mu}M$ in TR-TBT 18d-1 and TR-TBT 18d-2, respectively. [$^3H$]Paclitaxel uptake was markedly inhibited by cyclosporine and verapamil, well-known substrates of P-glycoprotein (P-gp) transporter. However, several MRP substrates and organic anions had no effect on [$^3H$]paclitaxel uptake in TR-TBT cells. These results suggest that P-gp may be involved in paclitaxel transport at the placenta. TR-TBT cells expressed mRNA of P-gp. These findings are important for therapy of breast and ovarian cancer of pregnant women, and should be useful data in elucidating teratogenicity of paclitaxel during pregnancy.

Magnetic and Temperature-Sensitive Composite Polymer Particles and Adsorption Behavior of Emulsifiers and Trypsin

  • Ahmad, Hasan;Rahman, M.Abdur;Miah, M.A. Jalil;Tauer, Klaus
    • Macromolecular Research
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    • v.16 no.7
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    • pp.637-643
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    • 2008
  • A combination of magnetic and temperature-responsive properties in the same polymer composites is expected to increase their potential applications in the biomedical field. Accordingly, micron-sized magnetite/polystyrene/poly(2-dimethylaminoethyl methacrylate-ethyleneglycol dimethacrylate), which are abbreviated as $Fe_3O_4$/PS/P (DM-EGDM) composite polymer particles, were prepared by the seeded copolymerization of DM and EGDM in the presence of magnetite/polystyrene ($Fe_3O_4$/PS) particles. $Fe_3O_4$/PS/P(DM-EGDM) composite particles with magnetic properties showed a temperature-sensitive phase transition at approximately $31^{\circ}C$. The adsorption behavior of the low molecular weight emulsifiers and trypsin (TR) as biomolecules were examined on $Fe_3O_4$/PS/P(DM-EGDM) composite polymer particles at different temperatures. The native conformation of TR was followed by measuring the specific activity under various adsorption conditions. The activity of the adsorbed TR on composite polymer particles was higher than those of the tree TR and TR adsorbed on $Fe_3O_4$/PS particles.

Transportation Management System for Logistics Company using Smart Virtual TRS (Smart Virtual TRS를 활용한 물류기업의 수배송관리시스템)

  • Lim, Yongtaek;Kim, Sunggyun;Yoo, Woosik
    • Journal of Information Technology and Architecture
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    • v.10 no.1
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    • pp.93-100
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    • 2013
  • The purpose of this paper is to present Smart Virtual TRS(Trunked Radio System) service that gives the TRS function in mobile network with smart phone application and server. TRS is essential equipment of logistics vehicle so, most of trunk drivers uses TRS frequently. Developed service is based on smartphone without TRS equipment. Therefore, Smart virtual TRS included in TMS(Transportation Management System) has some effects for logistics company. i) Smart Virtual TRS gives better communication environment between office and drivers. ii) Smartphone App gives flexibility to TMS functions. iii) Smart Virtual TRS gives cost reduction effect.

T1-, T2-weighted, and FLAIR Imaging: Clinical Application (T1, T2강조영상, FLAIR영상의 임상 적용)

  • Kim, Jae-Hyoung
    • Investigative Magnetic Resonance Imaging
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    • v.13 no.1
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    • pp.9-14
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    • 2009
  • T1-, and T2-weighted imagings and FLAIR (fluid attenuated inversion recovery) imaging are fundamental imaging methods in the brain. T1-weighted imaging is a spin-echo sequence with short TR and short TE and produces the tissue contrast by different T1 relaxation times. In other words, short TR maximizes the difference of the longituidinal magnetization recovery between the tissues. T2-weighted imaging is a spin-echo sequence with long TR and long TE and produces the tissue contrast by different T2 relaxation times. Long TE maximizes the difference of the transverse magnetization decay between the tissues. FLAIR is an inversion recovery sequence using 180 degree inversion pulse. 2500 msec of inversion time is applied to suppress the CSF signal.

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Effects of Bridging Exercise using Vibration Stimulation and ADIMs on the Lengthening and Thickness of Transversus Abdominis in Healthy Adults

  • Kong, Kwan-Woo
    • The Journal of Korean Physical Therapy
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    • v.28 no.6
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    • pp.393-397
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    • 2016
  • Purpose: This study was conducted in an effort to determine the effects of various abdominal drawing-in maneuver (ADIM) on the thickness and length of the transversus abdominis (TrA) when using lumbar stabilization exercises on healthy adults. Methods: 72 healthy adults were divided into four groups of 18 subjects each, to which different ADIM methods were applied. 1) a simple ADIM exercise, 2) an ADIM with pressure bio-feedback units, 3) an ADIM exercise with sling, and 4) an ADIM exercise with sling and vibration. Changes in the thickness and sliding length of TrA were measured when ADIM was conducted in the supine position prior to exercise and again when beginning the exercises. Following exercise, changes in the thickness and sliding length of TrA were measured using the same methods. Differences in group measurements prior to and following exercise were compared using a one-way analysis of variance. A paired t-test was applied to compare the before and after differences within each group. Results: Differences in TrA thickness change revealed that the ADIM exercise with sling and vibration group showed a significant difference in measurements taken prior to and following exercise. Differences in TrA length change revealed that the ADIM exercise with sling and vibration group showed a significant difference in measurements taken prior to and following exercise. Conclusion: ADIM exercise with vibration stimulation conducted in the bridge posture while in a prone position using a sling can be recommended as an effective exercise to improve the function of lumbar TrA.

Transmission ritual in the Huangdi Neijing : What effect did the form of knowledge transmission have on medicine? (『황제내경(黃帝內經)』의 전승의례(傳承儀禮) : 지식(知識)의 전달형태(傳達形態)가 의학(醫學)에 어떤 영향(影響)을 미쳤는가?)

  • Song, Seok Mo
    • Journal of Korean Medical classics
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    • v.34 no.3
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    • pp.55-72
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    • 2021
  • Objectives : This paper investigates the significance of the transmission ritual (TR) documented in the Huangdi Neijing (HN) and its effects on medicine pursued by HN's authors (medical bureaucrats). Methods : Descriptions of knowledge transmission and related ritual were identified from HN texts. They were compared with similar types of actions in ancient China, then historically reconstructed. They were also approached from the perspective of ritual studies. Results : It appears highly probable that TR in HN had actually been performed by the authors themselves, which shows characteristics of rites of passage. The participants accepted the in-group order based on patrimonial piety. TR consecrates the participants as well as its object, which is medical knowledge. Conclusions : In the field of TR, the participants shared not only a specific document or knowledge, but also their common experiences of medical perfection and sanctity. They constructed their noble group identity through TR. At the same time, TR inhibited arguments inside the group, in favor of one-way communication.

Changes in Plasma Steroid Hormone Level in Rockfish (Sebastes inermis) by the Controlled Water Temperature and Photoperiod (수온과 광주기 조절에 의한 볼락 (Sebastes inermis)의 혈장내 성 스테로이드호르몬 농도의 변화)

  • CHANG Young Jin;LIM Han Kyu;KWON Joon Yeong
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.34 no.1
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    • pp.13-16
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    • 2001
  • Plasma levels of sex steroid hormones in rockfish, Sebastes inermis were examined monthly in relation to gonadosomatic index (GSI) under a controlled water temperature and photoperiod, The GSI of a control group (C) in female began to increase from November and reached a maximum in January, Sample fish under a controlled water temperature and photoperiod (Tr) were divided into a responded group (Tr-r) and a un-responded group (Tr-n) by the gonadal maturation condition and GSI. The GSI of females in Tr-r reached a maximum in March. But the female GSI in Tr-n kept lower than 1.2 during the experimental period. No differences in male GSI were noticed between C and Tr. The $estradiol-17\beta$ and testosterone levels of female plasma in Tr reached a maximum in October, later than those in C. In males, these was no difference in 11-ketotestosterone and testosterone between C and Tr. When rockfish was reared in September under the controlled water temperature and photoperiod which were equivalent to those in July, that is two months earlier, the maturation of females was delayed in comparison with C. This finding suggested that delayed maturation in ovary was caused by the secretion of sex steroid hormones in relation to the water temperature and photoperiod of the hypothalamus-pituitary-gonad axis.

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Comparative Expression of the Aedes aegypti 5-Hydroxytryptamine7 Receptor in Drosophila Schneider2 and Chinese Hamster Ovary-K1 Cells (초파리 Schneider2 세포와 Chinese hamster ovary-K1 세포에서 Aedes aegypti 5-Hydroxytryptamine7 수용체의 발현비교)

  • 이대원
    • Korean journal of applied entomology
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    • v.43 no.2
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    • pp.155-162
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    • 2004
  • Serotonin receptor binds to serotonin (5-HT) and activates effector proteins such as adenylyl cyclase, phospholipase C, cyclic GMP phosphodiesterase or ion channel through G protein on the cell membrane, resulting in various physiological responses like diuresis, memory and development. To examine the comparative expression of the 5-HT$\_$7/ receptor of Aedes aegypti, the Aedes 5-HT$\_$7/ receptor gene was transfected into Drosophila Schneider2 (S2) cells and mammalian Chinese hamster ovary (CHO)-Kl cells. The expression of the Aedes 5-HT$\_$7/ receptor gene in selected cell lines, Tr-CHO and Tr-S2, was confirmed with reverse transcription-PCR, Western blot and immunocytochemistry. Compared with the induced intracellular cAMP level of Tr-S2 cell line to 5-HT, the induced cAMP in the Tr-CHO cell line was over 9 times higher and was dose-dependent. These results suggest that the functionality of Aedes 5-HT$\_$7/ receptor is much more effective in mammalian CHO-K 1 cells and that the Tr-CHO cell line expressing Aedes 5-HT$\_$7/ receptor can be used for synthetic agonist or antagonist candidate screening.