• Title/Summary/Keyword: TEAC

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Contents of Isoflavones and Antioxidative Related Compounds in Soybean Leaf, Soybean Leaf Jangachi, and Soybean Leaf Kimchi (콩잎 밑 콩잎 요리의 이소플라본 함량 및 항산화 관련 성분들의 비교)

  • Ryu Seung-Hee;Lee Hye-Suk;Lee Young-Soon;Moon Gap-Soon
    • Korean journal of food and cookery science
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    • v.21 no.4 s.88
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    • pp.433-439
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    • 2005
  • Soybean is an important plant as it is the source of protein and oil as well as various phytochemicals that are related with biological activity. Over the past decades, scientists have conducted considerable research on the physiological properties of soybeans, especially isoflavones, which are the characteristic components in soybeans. However, there is no research on the properties or the bio-functionality of soybean leaf. Jangachi and kimchi are two of the traditional special dishes of Gyungsang Province in Korea which we made from soybean leaves. Depending on the recipe, green or yellow soybean leaves are used for the preparation of these two side dishes. We compared the antioxidative activity and measured the contents of isoflavones, total phenol, chlorophylls, carotenoids, and vitamin C in the ingredients (green and yellow soybean leaf) and the final side dishes (jangachi and kimchi). We Int report that isoflavones were contained in soybean leaf and that jangachi had the highest isoflavone contents among the samples. Yellow soybean leaf contained higher isoflavones than green soybean lear and kimchi. From the TEAC assay results, the sequence or antioxidative activities was yellow soybean leaf > soybean leaf jangachi > green soybean leaf > soybean leaf kimchi. The sequence was the same with total phenol contents, indicating that antioxidative activity is highly related with total phenol level. Chlorophylls, carotenoids and vitamin C existed abundantly in green soybean leaf. In conclusion, soybean leaf could be a good material for health due to the presence of isoflavones and the other useful antioxidants mentioned above.

Empathy Evaluation Method Using Micro-movement (인체 미동을 이용한 공감도 평가 방법)

  • Hwang, Sung Teac;Park, SangIn;Won, Myoung Ju;Whang, Mincheol
    • Science of Emotion and Sensibility
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    • v.20 no.1
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    • pp.67-74
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    • 2017
  • The goal of this study is to present quantification method for empathy. The micro-movement technology (non-contact sensing method) was used to identify empathy level. Participants were first divided into two groups: Empathized and not empathized. Then, the upper body data of participants were collected utilizing web-cam when participants carried expression tasks. The data were analyzed and categorized into 0.5 Hz, 1 Hz, 3 Hz, 5 Hz, 15 Hz. The average movement, variation, and synchronization of the movement were then compared. The results showed a low average movement and variation in a group who empathized. Also, the participants, who empathized, synchronized their movement during the task. This indicates that the people concentrates with each other when empathy has been established and show different levels of movement. These findings suggest the possibility of empathy quantification using non-contact sensing method.

Engagement classification algorithm based on ECG(electrocardiogram) response in competition and cooperation games (심전도 반응 기반 경쟁, 협동 게임 참여자의 몰입 판단 알고리즘 개발)

  • Lee, Jung-Nyun;Whang, Min-Cheol;Park, Sang-In;Hwang, Sung-Teac
    • Journal of Korea Game Society
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    • v.17 no.2
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    • pp.17-26
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    • 2017
  • Excessive use of the internet and smart phones have become a social issue. The level of engagement has both positive and negative effects such as good performance or indulgence phenomenon, respectively. This study was to develop an algorithm to determine the engagement state based on cardiovascular response. The participants were asked to play a pattern matching game and the experimental design was divided into cooperation and competition task to provide the level of engagement. The correlation between heart rate and amplitude was analyzed according to each task. The regression equation and accuracy were verified by polynomial regression analysis. The results showed that heart rate and amplitude were positively correlated when the task was a game, and negatively correlated when there was a reference task. The accuracy of classifying between game and reference task was 89%. The accuracy between tasks was confirmed to be 76.5%. This study is expected to be used to quantitatively evaluate the level of engagement in real time.

Research on Emotional Convergence Technology based on Non Contact Sensing and Social Interaction. (비접촉 센싱 기술과 사회적 상호작용에 따른 감성융합기술 연구)

  • Hwang, Sung Teac;Whang, Mincheol
    • Journal of the Korea Convergence Society
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    • v.8 no.6
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    • pp.163-170
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    • 2017
  • In this paper want to recognize competition and cooperation as a non-contact sensing method among the social emotion that are caused by interaction. Experiments were conducted in a pattern matching task divided into competition and cooperation situations. Data collection was performed by extracting the micro-movement data of 15Hz component from the upper body image. We compared and analyzed the amount and the variation and synchronization. As a result, Amount and variation are bigger in competition than cooperation. Synchronization was higher in cooperation than competition. This can be seen as the difference between two people trying to win when they are in competition and trying to breathe each other in cooperation. This study is significant in confirming the possibility of competition and cooperation through non contact sensing method based on physiological response.

Suppressive Effect of FARFARE FLOS Extracts on Oxidative Stress and Inflammatory Response through the Antioxidative Mechanism (FARFARE FLOS의 항산화 기전을 통한 산화적 스트레스 및 염증 반응 억제효과)

  • Shin, Seung-An;Lee, Min-Ja;Lee, Hye-Sook;Park, Won-Hwan
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.25 no.2
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    • pp.280-293
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    • 2011
  • There is currently increased interest in the identification of antioxidant compounds that are pharmacologically potent and have low or no side effects. Plants produce significant amounts of antioxidants to prevent the oxidative stress caused by photons and oxygen, therefore they represent a potential source of new compounds with antioxidant activity. FARFARE FLOS has been frequently used on the respiratory system including bronchitis, phthisis. In this study, the antioxidant activity of extract from FF was studied in vitro methods by measuring the antioxidant activity by TEAC, measuring the scavenging effects on reactive oxygen species (ROS) [superoxide anion, hydroxyl radical] and on reactive nitrogen species (RNS) [nitric oxide and peroxynitrite] as well as measuring the inhibitory effect on Cu2+-induced human LDL oxidation. The FF extracts were found to have a potent scavenging activity, as well as an inhibitory effect on LDL oxidation. And this study was designed to evaluate whether FFEA may ameliorate oxidative stress and inflammatory status through the antioxidative mechanism in LPS-stimulated RAW 264.7 murine macrophage cell line. Treatment of RAW 264.7 cells with FFEA significantly reduced LPS-stimulated inflammatory response in a dose-dependent manner. In conclusion, the FF extracts have anti-oxidative and anti-inflammatory effects in vitro system, which can be used for developing pharmaceutical drug against oxidative stress and atherosclerosis.

Anti-hypertensive Effects of ethanol extract of Phyllostachys Pubescens via Antioxidant Activity (맹종죽의 항산화활성을 통한 항고혈압 효능)

  • Lee, Hye-Suk;Park, Min-Hee;Kim, Jung-Suk;Lim, Beong-Ou;Moon, Gap-Soon;Shin, Heung-Mook
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.21 no.3
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    • pp.658-665
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    • 2007
  • Phyllostachys pubescens (Maengiong-Juk), a kind of the bamboo, was reported to have many beneficial pharmacological actions. in this study, of using 70% ethanol extract of Phyllostachys pubescens we investigated its efficacy on angiotensin converting enzyme (ACE) and antioxidant enzyme activities. In addition, vasorelaxant effect was examined in rat aortic rings. The inhibitory effect of ACE activity by Phyllostachys pubescens extract (PPE) was dose-dependently increased by 61.42% at 10mg/ml. PPE relaxed the pre-contracted rat aortic rings with 10$^{-6}$M phenylephrine, showing about 88% at 4.0mg/ml. Sprague Dawley (SD) rats were given different concentrations of PPE mixed in the drinking water for 10 weeks. PPE did not show any difference with control group in blood pressure, body weight (BW) and food intake. However, it revealed the highest total antioxidative effect at dose of 1.0 g/100 g BW in plasma by TEAC assay. Thiobarbituric acid reactive substance (TBARS) and protein carbonyl levels which are markers of tissue peroxidation, were significantly lowed at the same dosage. Furthermore, hepatic antioxidant enzymes such as total superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and catalase activities were also significantly increased by PPE (1.0 g/100 g BW). In conclusion, we suggest that PPE might have antihypertensive effect through increasing antioxidant activities.

Synthesis of Tricyclopentadiene Using Ionic Liquid Supported Mesoporous Silica Catalysts (이온성 액체가 담지된 메조포로스 실리카 촉매를 이용한 Tricyclopentadiene 합성)

  • Kim, Su-Jung;Jeon, Jong-Ki;Han, Jeongsik;Yim, Jin-Heong
    • Applied Chemistry for Engineering
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    • v.27 no.2
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    • pp.190-194
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    • 2016
  • Tricyclopentadiene (TCPD) is one of the important precursors for making tetrahydrotricyclopentadiene, which is well known as a next-generation fuel with high energy density. In this study, TCPD was obtained by polymerization reaction of dicyclopentadiene (DCPD) using an ionic liquid (IL) supported mesoporous silica catalysts. ILs were supported to two kinds of mesoporous silica catalysts with different pore sizes such as MCM-41 and SBA-15. Four different ILs were supported to mesoporous silicas using anionic precursors such as CuCl or $FeCl_3$ and cationic precursors such as triethylamine hydrochloride or 1-butyl-3-methylimidazolium chloride. We proved that IL supported mesoporous silicas showed better catalytic performance than those of using non-supported prestine IL in the aspect of TCPD yield and DCPD conversion. Among four kinds of IL supported mesoporous silica catalysts, CuCl-based IL supported MCM-41 system showed the highest TCPD yield.

Hyaluronidase Inhibitory and Antioxidant Activities of Enzymatic Hydrolysate from Jeju Island Red Sea Cucumber (Stichopus japonicus) for Novel Anti-aging Cosmeceuticals

  • Ding, Yuling;Jiratchayamaethasakul, Chanipa;Kim, Eun-A;Kim, Junseong;Heo, Soo-Jin;Lee, Seung-Hong
    • Journal of Marine Bioscience and Biotechnology
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    • v.10 no.2
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    • pp.62-72
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    • 2018
  • An active ingredient with hyaluronidase (HAse) inhibitory effect is one of the anti-aging approaches in cosmeceuticals. Here, red sea cucumbers (RSCs), Stichopus japonicus, from Jeju Island were evaluated to examine their HAse inhibitory and antioxidant activity effect. In this study, RSCs were extracted by six enzymatic hydrolysis (Alcalase; Al, Trypsin; Try, Neutrase; Neu, Pepsin; Pep, Alpha-chymotrypsin; Chy and Protamex; Pro). Alcalase hydrolysate (AlH) showed the highest antioxidant capacities for both of oxygen radical absorbance capacity (ORAC) and trolox equivalent antioxidant capacity (TEAC) methods, compared to those of other hydrolysates, at $66.59{\pm}0.78{\mu}M\;TE/mg$ and $135.78{\pm}3.24{\mu}M\;TE/mg$, respectively. Furthermore, AlH performed the highest capacity of HAse inhibitory with $IC_{50}$ value of 3.21 mg/ml. Thus, RSCs hydrolyzed by Al were chosen to determine the cellular antioxidant activity and hyaluronic acid (HA) production effect on Human immortalized keratinocyte cell line (HaCaT). The results showed that AlH improved the cell viabilities and intracellular reactive oxygen species (ROS) induced by 2,2'-Azobis(2-amidinopropane) dihydrochloride (AAPH) were significantly decreased. In addition, AlH increased HA amount by regulating HYAL2 and HAS2 expressions in the HaCaT cells. Taken together, AlH of RSCs collected from Jeju Island showed HAse inhibitory and antioxidant activities against skin-aging which shows its potentials can be an optional natural bioactive ingredient for novel cosmeceuticals.

Antioxidant activity and phenolic acid content of Gynostemma pentaphyllum leaves according to extraction conditions

  • Ko, Hyun Min;Eom, Tae Kil;Kim, Ju-Sung
    • Korean Journal of Agricultural Science
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    • v.46 no.1
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    • pp.85-92
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    • 2019
  • This study was intended to provide basic data for a health functional food study by exploring antioxidant activity of reflux extract according to the concentration of ethanol and the extract of ultrasonic waves extracted and reflux extracted under the same solvent conditions. In the same solvent condition, the reflux extract ($75.10{\pm}1.99mg$) showed a higher total phenol content than the ultrasonic wave extract ($51.74{\pm}2.28mg$). Flavonoids also had a higher reflux extract ($25.05{\pm}1.53mg$) than did ultrasonic extracts ($16.23{\pm}1.95mg$). Reflux extract according to ethanol concentration was found to have a higher phenol content than the 70% ethanol extract ($40.60{\pm}1.49mg$) in 60% ethanol extract. Flavonoid content was also similar to phenol content in reflux extract as determined by ethanol concentration from 60% ethanol ($25.05{\pm}1.53mg$) to 70% ethanol extract ($6.60{\pm}0.46mg$). In addition, the antioxidant activity (DPPH, TEAC, FRAP, ORAC) of the reflux extract in the same solvent conditions tended to be higher than that of ultrasonic extracts. Also, 60% ethanol extract had better antioxidant activity than 70% ethanol extract. However, an analysis of phenolic acid content through HPLC showed that the ultrasonic extract had a higher content in the same solvent condition than did the reflux extract. Not only the presence of phenolic acid, but also those of other compounds are believed to be attributed to the activity of antioxidants. Therefore, further studies are needed to clarify this phenomenon.

Manufacturing and Quality Characteristics Analysis of Coffee Powder with Added Hallabong Extract (한라봉 추출액이 첨가된 커피 분말의 제조 및 품질 특성에 관한 연구)

  • Shin, Kyung-Ok;Ha, Seo-Yeong;Shin, Seong-Beom;Kim, Jeong-Yeon;Yang, Ming
    • The Korean Journal of Food And Nutrition
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    • v.34 no.6
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    • pp.593-603
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    • 2021
  • In this study, Korean Hallabong produced in Jeju Island and coffee were grafted to prepare coffee containing Hallabong extract and the nutritional components were analyzed. As the amount of Hallabong extract increased, the water content and total polyphenol content increased. However, the crude flour, crude protein, and total flavonoid content decreased significantly. The selenium content per 100 g was 91.28 mg in the 1% Hallabong group, and the iron content was 6.84 mg in the 3% Hallabong group. As the content of Hallabong extract in coffee increased, the L-value (brightness) and b-value (yellowness) increased, but the a-value (redness) showed a tendency to decrease. In the case of DPPH(2,2-Diphenyl-1-picrylhydrazyl) radical scavenging activity, the group containing 9% of Hallabong extract showed the highest value at 47.20 μmol/g of TEAC. In particular, the ABTS(2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonate)) and DPPH radical scavenging activity were significantly increased from coffee powder containing 6% or more of Hallabong extract(p<0.05). The caffeine content decreased as the amount of Hallabong extract added to coffee increased. Therefore, when making powder coffee with Hallabong extract added, it is recommended to set the content of Hallabong extract to 6%.