• Title/Summary/Keyword: TA-4

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Elucidation of Anti-tumor Initiator and Promoter Derived from Seaweed-2: Investigation of Seaweed Extracts Suppressing Mutagenic Activity of PhIP and MeIQx (해조류 중의 anti-tumor initiator 및 promoter의 해석-2: PhIP와 MeIQx의 돌연변이원성을 억제하는 해조 추출물 소재의 연구)

  • PARK Young-Beom;KIM In-Soo;YOO Sung-Jae;AHN Jong-Khan;LEE Tae-Gee;PARK Douck-Chon;KIM Seon-Bong
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.31 no.4
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    • pp.581-586
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    • 1998
  • The present study was conducted to prepare seaweed extracts suppressing mutagenic activity of 2-amino-1-methyl-6-phenylimidazo[4,5-b] pyridine ( PhIP ) ana 2-amino-3,8-dimethylmidazo[4,5-f]quinoxaline (MeIQx) derived from cooked meat produce. The tumor initiation activity of PhIP and MeIQx was assayed with Ames method using Salmonella typhimurium TA98 in the presence of S-9 mixtures before and after addition of methanol-solubles of seaweed, such as, Phaeophyta; Undaria pinnatifida, Ecklonia stolonifera Ecklonia cava, Laminaia japonica Sargassum fulvellum, Sargassum hornezi, Sargassum miyabei, Sargassum thunbergii, Agarum cribrosum and Hizikia fusifomis, Rhodophpei Porphyra yezoensis, Grateloupia eiliptiut Lomentraria catenata, Ploemium telfairiae and Glarilaria verrucosa, Chlorophyta; Codium fragile, Enteromorpha compresa and Ulva pertusa. Among seaweed tested, Phaeophyta was shown the higher desmutagenic activity than Rhodophyta and Chlorophyta. E. stolonifera, E. cava and S. miyabei, among Phaeophyta exerted the stronger desmutagenic activity (above $90\%$/2 mg). The ethyl acetate, diethyl ether and chlomform extracts except water extracts from E. stolonifera exhibited a high desmutagenic activity. The ethyl acetate extract of E. stolonifera which showed highest activity was fractionated with Sephadex LH20 column chromatography to give active fraction A-7, which showed desmutagenicity of $90\%$/mg against PhIP and $80\%$/mg against MeIQx. The active fraction had the absorbance at 207.7 and 232nm.

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Effects of Social Work Field Practicum Related Factors on Empowerment of Students Majoring in Social Welfare (사회복지현장실습 관련 요인이 사회복지전공학생들의 임파워먼트에 미치는 영향)

  • Park, Soon-Mi;Son, Ji-A
    • The Journal of the Korea Contents Association
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    • v.18 no.12
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    • pp.558-569
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    • 2018
  • The purpose of this study is examine the effect of social work field practicum related factors on empowerment of students majoring in social welfare. For this purpose, a survey of the university students with completing social work field practicum was conducted and data was analyzed. The results of this study are summarized as follows: The empowerment level of the students with completing social work field practicum is 3.71. Multiple regression analysis was conducted for each sector by dividing the factors related to the social welfare field practicums affecting the empowerment into individual factors, practical institutional factors, and school factors. Degrees of practice(${\beta}=.123$, t=2.405, p<.05), the satisfaction level of the supervisor(${\beta}=.176$, t = 2.446, p <.05), the lecture satisfaction level of the practicum(${\beta}=.229$, t=4.018, p<.001) were found to affect the empowerment in each sector. But in final analysis with all factors have been concerned, the lecture satisfaction level of the practicum(${\beta}=.257$, t=4.495, p<.001) had a positive effect on the empowerment. Based on these results, implications for research and practice were discussed.

Effects of Elevated Spring Temperatures on the Growth and Fruit Quality of the Mandarin Hybrid 'Shiranuhi' (봄철 가온처리가 부지화의 생장과 과실품질에 미치는 영향)

  • Moon, Young-Eel;Kang, Seok-Beom;Han, Seung-Gab;Kim, Yong-Ho;Choi, Young-Hun;Koh, Seok Chan;Oh, Soonja
    • Horticultural Science & Technology
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    • v.33 no.4
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    • pp.459-469
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    • 2015
  • The effects of elevated spring temperatures on the growth and fruit quality of the mandarin hybrid 'Shiranuhi' [(Citrus unshiu ${\times}$ C. sinensis) ${\times}$ C. reticulata] were investigated in plastic greenhouses, to develop a cropping system to improve the quality of the fruit and increase the income of growers on Jeju Island, South Korea. Under conditions of elevated temperature I ($25/15^{\circ}C$, day/night) and elevated temperature II ($28/18^{\circ}C$, day/night) during early spring, budburst was advanced by 11 and 15 d, and full bloom by 22 and 45 d, respectively, compared to those of the plants grown at ambient air temperature in a plastic greenhouse. Elevated temperatures decreased the number of spring shoots but increased mean spring shoot length and leaf area. Growing 'Shiranuhi' trees at elevated temperatures resulted in increases in mean fruit weight and fruit L/D ratio (> 1.0). In addition, fruit color development was significantly advanced in trees grown under elevated temperatures during early spring, which allowed the fruit to be harvested 1-2 months earlier than trees grown under ambient air temperature. Fruit soluble solids content (SSC) and titratable acidity (TA) at harvest were similar between elevated temperature I and ambient air temperature, but were significantly higher than at elevated temperature II. Considering fruit quality, harvest time, and yield, the elevated temperature treatment regime of $25/15^{\circ}C$ (day/night) during early spring could be useful for cultivation of the mandarin hybrid 'Shiranuhi' to increase the income of growers.

Nocturnal Bruxism and Botulinum Toxin Effect on the Subjects with Masseteric Hypertrophy (야간 이갈이와 교근비대 환자의 보툴리눔 독소 주사 효과)

  • Sohn, Seung-Mahn;Chung, Gi-Chung;Kim, Mee-Eun;Kim, Ki-Suk
    • Journal of Oral Medicine and Pain
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    • v.32 no.3
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    • pp.337-346
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    • 2007
  • This study aimed to evaluate a relation of bruxism with clinical effects of botulinum toxin type A(BTX-A) injection. 5 bruxers and 5 nonbruxers with bilateral masseter hypertrophy were participated in this study. After injecting 25 unit of BTX-A(Allergen Inc, $Botox^{(R)}$) into each masseter muscle, the thickness of masseter(Mm) and anterior temporalis(Ta) muscles was measured by ultrasonography and the maximum bite force was evaluated during a 9-month period. Self-estimation on the recovery of occlusal force during mastication was done as well. Regardless of presence of bruxsim, all subjects showed significantly reduced Ms thickness(p<0.001) and maximum bite force at $1^{st}$ molars(p=0.027) with their peak at 3 months after injection, which then started to return. No significant difference was observed in Ta thickness and the bite force at the central incisors. While self-estimated occlusal force was the least at 2 weeks after injection and then rapidly returned to the baseline level with full recovery at the time of 6 to 9 months after injection, the maximum bite force measured by bite force recorder did not recover the original value, particularly in the nonbruxer group. It is assumed that nocturnal bruxism can influence recovery of atrophic masseter and decreased occlusal force due to BTX-A injection. These findings suggest a need of occlusal appliance to control bruxism or clenching habit for longer clinical effect of BTX-A injection.

Comparative Study of Total Acidity Content of Makgeolli with Pre-Treatment and Analysis Methods (전처리과정과 분석방법에 따른 막걸리의 총산 함량 비교)

  • Kim, Ye Seul;Shin, Kwang Seup;Lee, Jeung Hee
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.46 no.5
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    • pp.600-607
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    • 2017
  • The total acidity (TA) of Makgeolli was affected by suspended solids and $CO_2$ produced during the fermentation process. Nine Makgeollis (four sterilized and five unsterilized Makgeollis) were collected in the market, and their TAs were compared before and after filtration and $CO_2$ removal. TAs of sterilized Makgeollis were 0.379~0.477%, which significantly decreased to 0.167~0.225% after filtration and 0.132~0.170% after $CO_2$ removal (P<0.05). TAs of unsterilized Makgeollis were 0.412~0.467% and decreased to 0.157~0.365% after filtration and 0.143~0.280% after $CO_2$ removal (P<0.05). TAs of Makgeollis were compared by three methods using different indicators. The TAs of sterilized and unsterilized Makgeollis were 0.105~0.123% and 0.105~0.200%, respectively, by bromthymol blue+neutral red (light green), 0.129~0.154% and 0.130~0.255%, respectively, by phenolphthalein (faint pink), and 0.120~0.146% and 0.130~0.232%, respectively, by bromthymol (blue). Nowadays, Makgeolli is commercialized with various distinct colors, and thus it is important to select appropriate indicators for proper titration endpoint identification for TA measurement. The compositions of organic acids profiles varied depending on sterilized or unsterilized Makgeollis, in which oxalic acid (0.108~0.329 mg/mL), malic acid (ND to 0.134 mg/mL), lactic acid (0.127~0.776 mg/mL), and citric acid (ND to 1.159 mg/mL) were found, and lactic acid was in unsterilized more than sterilized Makgeollis.

Genotoxicological Safety of Hot Water Extracts of the ${\gamma}$-Irradiated Glycyrrhizae Radix, Aurantii nobilis Pericarpium and Bupleuri Radix in vitro (감마선조사 감초, 진피(陳皮) 및 시호 열수 추출물의 in vitro 유전독성학적 안전성 평가)

  • Jo, Sung-Kee;Ham, Yeon-Ho;Park, Hae-Ran;Oh, Heon;Byun, Myung-Woo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.30 no.6
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    • pp.1137.2-1245
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    • 2001
  • The ${\gamma}$-irradiated medicinal herbs were examined the genotoxicological safety to consider the possibility of application of the irradiation technology for hygienic purpose. The three medicinal herbs -Glycyrrhigae Radix, Aurantii nobilis Pericarpium and Bupleuri Radix- were irradiated with ${\gamma}$ -rays at the practical dosage of 10 kGy. The hot water extracts of the irradiated herbs were examined in two short-term in vitro tests: (1) Ames test in Salmonella typhimurium TA98 and TA100, (2) Micronucleus test in cultured Chinese hamster ovary(CHO) cells. In the Salmonella reversion assays both with and without metabolic activation, the number of revertant colonies was not increased with each extract from the irradiated herbs, compared with negative controls. No significant difference in formation of the colonies was observed between non-irradiated and 10 kGy-irradiated herbs. These results indicated that no mutagenicity of the irradiated herbs was detected. In the micronucleus tests in cultured CHO cells, the incidences of micronucleus were not increased with irradiated herbs, and no significant difference in the incidences was observed between non-irradiated and irradiated herbs. These results indicated that no cytogenetic toxicity of the irradiated herbs was detected. The results of the two in vitro tests suggest that the irradiated herbs do not show mutagenic effects and cytogenetic toxicity. Further tests of in vivo genotoxicity and chronic toxicity are needed to determine the safety of the herbs irradiated with ${\gamma}$ -rays at practical doses.

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Bias Voltage Dependence of Magnetic Tunnel Junctions Comprising Double Barriers and CoFe/NiFeSiB/CoFe Free Layer (CoFe/NiFeSiB/CoFe 자유층을 갖는 이중장벽 자기터널접합의 바이어스전압 의존특성)

  • Lee, S.Y.;Rhee, J.R.
    • Journal of the Korean Magnetics Society
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    • v.17 no.3
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    • pp.120-123
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    • 2007
  • The typical double-barrier magnetic tunnel junction (DMTJ) structure examined in this paper consists of a Ta 45/Ru 9.5/IrMn 10/CoFe7/$AlO_x$/free layer/AlO/CoFe 7/IrMn 10/Ru 60 (nm). The free layer consists of an $Ni_{16}Fe_{62}Si_8B_{14}$ 7 nm, $Co_{90}Fe_{10}$ (fcc) 7 nm, or CoFe $t_1$/NiFeSiB $t_2$/CoFe $t_1$ layer in which the thicknesses $t_1$ and $t_2$ are varied. The DMTJ with an NiFeSiB-free layer had a tunneling magnetoresistance (TMR) of 28%, an area-resistance product (RA) of $86\;k{\Omega}{\mu}m^2$, a coercivity ($H_c$) of 11 Oe, and an interlayer coupling field ($H_i$) of 20 Oe. To improve the TMR ratio and RA, a DMTJ comprising an amorphous NiFeSiB layer that could partially substitute for the CoFe free layer was investigated. This hybrid DMTJ had a TMR of 30%, an RA of $68\;k{\Omega}{\mu}m^2$, and a of 11 Oe, but an increased of 37 Oe. We confirmed by atomic force microscopy and transmission electron microscopy that increased as the thickness of NiFeSiB decreased. When the amorphous NiFeSiB layer was thick, it was effective in retarding the columnar growth which usually induces a wavy interface. However, if the NiFeSiB layer was thin, the roughness was increased and became large because of the magnetostatic $N{\acute{e}}el$ coupling.

Study on the Copper-Arsenic Green Pigments used on Shamanic Paintings in the 19~20th century (19~20세기 무신도 등에 사용된 구리-비소 녹색 안료에 대한 연구)

  • Oh, Joon Suk;Choi, Jung Eun;Choi, Yoon Hee
    • Journal of Conservation Science
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    • v.31 no.3
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    • pp.193-214
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    • 2015
  • The green pigments of shamanic paintings (83 items) in the 19~20th century were analyzed with X-ray fluorescent spectrometer(XRF), scanning electron microscopy (SEM) and fourier transform infrared spectroscopy (ATR-FTIR). In 60 items, copper and arsenic were detected in green pigments of the paintings by XRF spectra. Cu $K{\alpha}$/As $K{\beta}$ (peak intensity ratio of copper and arsenic) of shamanic paintings of Bokgaedang (shrine), solemn paintings (romance of three kingdoms) of Donggwanwangmyo and unknown enshrined place were 5.93~12.04 and higher compared to 5.67-6.26 of standard emerald greens and 4.01~7.89 of remaining shamanic paintings. The SEM images of crystal forms of copper-arsenic green pigments were various. Crystal forms were divided into oval and round spherulite with intersecting plate crystals and spherulite with agglomerate plate crystals. The crystals of the latter were found in shamanic paintings of Bokgaedang (shrine), solemn paintings (romance of three kingdoms) of Donggwanwangmyo and unknown enshrined place and the former were found in the rest of shamanic paintings. Copper-arsenic green pigments of shamanic paintings were identified as Scheele's green from shamanic paintings of Bokgaedang (shrine) and romance of three kingdoms. Emerald green from the rest of shamanic paintings by ATR-FTIR. From analytical results, it is confirmed that Scheele's green of shamanic paintings of Bokgaedang and romance of three kingdoms was used in the 1850s~1870s and emerald green had been widely used from late 19th century to 1970 in the rest of shamanic paintings.

PHOTOELASTIC ANALYSIS OF STRESS INDUCED BY DIFFERENT TYPE ENDOSSEOUS IMPLANTS (골내 임플랜트의 종류에 따른 광탄성 응력 분석)

  • Chung Chae-Heon;Chang Doo-Ik
    • The Journal of Korean Academy of Prosthodontics
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    • v.31 no.4
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    • pp.661-678
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    • 1993
  • The purpose of this study was to analyze the stress distribution at supporting bone according to the types of endosseous implants. This investigation evaluated the stress patterns in rectangular photoelastic models produced by four different types of dental implants such as $Br\ddot{a}nemark$, screw type of Steri-Oss, blade type of Steri-Oss, IMZ with IMC and resin tooth using the techniques of quasi-three dimensional photoelasticity. All prostheses were casted in the same nonprecious alloy and were cemented or screwed on their respective implants and abutments. 20 kg of vertical load was applied on the central fossa of casted crown and 16 kg of inclined had was applied on the top third of distal surface of casted crown respectively. The results were as follows : 1. Under the vertical load, screw implants of Steri-Oss and $Br\ddot{a}nemark$ showed increasing stress condition between and around the screw threads along the implant lateral surface and cylindrical implant of IMZ showed the less stress condition along the lateral surface with concentration of stress mostly near the root apex. 2. Under the vertical load, the stress of Steri-Oss blade was distributed uniformly at the alveolar bone under the broad blade. 3. Under the inclined load, the stress concentration of Steri-Oss screw and $Br\ddot{a}nemark$ was developed highly around the mesiocervical bone area on the contralateral side to force application. The stress of $Br\ddot{a}nemark$ with flexible gold glod was more concentrated in the cervical bone area than that of Steri-Oss with stiff screw. 4. Under the inclined load, the stress of Steri-Oss blade broadly was distributed around the mesioceivical bone area and the lower and mesial bone area of the blade. 5. Under the Inclined load, IMZ implant showed the gap between c개wn and fixture due ta deformation of the IMC and IMZ was lower in stress concentration developed around the mesiocervical bone area than $Br\ddot{a}nemark$ and Steri-Oss screw. 6. Under the inclined load, the stress magnitude induced in the mesiocervical bone area of implants was in order of $Br\ddot{a}nemark$, Steri-Oss strew, IMZ and Stsri-Oss blade. 7. Tilting forces as compared to axial forces exerted greater magnitude of stress in the cervical bone area of the implant. 8. In respect of stress distribution, Steri-Oss blade was superior than any other implants and in respect of the stability by horizontal lone, IMB and $Br\ddot{a}nemark$ was inferior than any other implants.

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Atmospheric concentration and mutagenicity of organic pollutants of suspended particulate in Seoul (서울시 대기중 유기오염물질의 농도와 돌연변이원성에 대한 연구)

  • Shin, Dong-Chun;Chung, Yong;Moon, Young-Hahn;Roh, Jae-Hoon
    • Journal of Preventive Medicine and Public Health
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    • v.23 no.1 s.29
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    • pp.43-56
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    • 1990
  • To evaluate the difference of concentration and mutagenicity of organic pollutants between residential and traffic area of Seoul, air samples were collected in Bulkwang (residential) and Shinchon (traffic) area. Samples were analyzed to measure the concentration of extractable organic matters (EOM) and their subfractions and mutagenicities were tested using Salmonella typhimurium TA 98. The concentrations of polycyclic aromatic hydrocarbons (PAHs) were also measured by gas-chromatography and compared between two areas. The results were as follows ; 1. While the concentration of total suspended particulate (TSP) in residential area was below the environmental standard in annual average, the concentration in traffic area was above the standard and was up to its maximum $256{\mu}g/m^3$ in November. The difference of TSP concentrations in both areas of each month was statistically significant (P<0.05). 2. The concentration of fine particle in traffic area was significantly higher compare to that in residential area and showed statistically significant monthly difference in both areas (P<0.05). The proportion of concentration of fine particle to TSP was 55-68%. 3. Mean concentrations of EOM in residential and traffic areas were $4.3{\mu}g/m^3\;and\;5.3{\mu}g/m^3$ respectively. The proportion of amount of EOM from fine particle to EOM from TSP was 70-88%. 4. While the percentage of polar neutral organic compounds (POCN) of fine particle in Bulkwang's sample was higher compare to Shinchon's sample, the percentage of aliphatic compounds of fine particle in Shinchon's sample was higher compare to Bulkwang's sample. The percentages of PAH fraction were as low as 6-10% in both areas. 5. The mutagenic activity of nit concentration of organic matters extracted from fine particle was higher compare to that of coarse particle and was increased when metabolically activated with S9. Mutagenicities with metabolic activation calculated by unit air volume were significantly different between residential and traffic area, $17\;revertants/m^3$\;and\;22\;revertants/m^3$ respectively. 6. The concentrations of benzo(a)pyrene in fine particle of traffic and residential areas were $3.10ng/m^3\;and\;2.02ng/m^3$ respectively. Sixteen PAHs were higher in samples of traffic area compare to residential area and also concentrations of PAHs in fine particle were higher compare to coarse particle.

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