• Title/Summary/Keyword: T500

Search Result 1,747, Processing Time 0.023 seconds

A Preliminary Study on Polyester Aluminum Bag as the Possible Substitute for Tedlar Bag Sampler in RSC Analysis (테들러 백 샘플러의 대체 소재로서 폴리에스터 알루미늄 백에 대한 예비연구: 환원황화합물을 중심으로)

  • Kim, Ki-Hyun;Jo, Sang-Hee
    • Journal of Korean Society for Atmospheric Environment
    • /
    • v.27 no.4
    • /
    • pp.454-459
    • /
    • 2011
  • In this study, the recovery rate of Tedlar bag (T) sampler was investigated in comparison to polyester aluminum bag (P) sampler. To derive the comparative data sets for the relative performance between different samplers, a series of calibration experiments were performed by using 1 ppb standard of four offensive reduced sulfur compounds (RSC) odorants ($H_2S$, $CH_3SH$, DMS, and DMDS) along with $SO_2$ and $CS_2$. All the analysis was made by gas chromatography/pulsed flame photometric detector (GC/PFPD) combined with air server/thermal desorber (AS/TD). The measurement data were obtained by loading gaseous standards (1 ppb) at 3 injection volumes (250, 500 and 1,000 mL) at three intervals (0, 24 and 72 hrs). The recovery rates (RR) of P sampler were computed against the slope values of T sampler. According to our analysis, P sampler exhibits slightly enhanced loss relative to T, especially with light RSCs ($H_2S$ and $CH_3SH$). At day 0, RR for the two were 88 and 85%, respectively. Such reduction proceeded rather rapidly in the case of $H_2S$ through time. However, P sampler was more stable to store $SO_2$ unlike others. Despite slightly reduced recovery, P sampler appears as a good replacement of T sampler.

Cell Viability and Hair Growth Effect on 3T3-L1 Cells of Ethanol Extract from Calendula officinalis L. Flower, Phellinus linteus Fruit Body and Houttuynia cordata Thunb. Whole Plant (금잔화, 상황, 어성초 에탄올 추출물의 세포독성 평가 및 3T3-L1 세포에 대한 육모 효과)

  • Jin, Seong Woo;Koh, Young Woo;Yun, Kyeong Won;Kim, Kyung Je;Je, Hae Shin;Im, Seung Bin;Kim, Kwang Sang;Kim, Min Sook;Yu, Byung Jo;Seo, Kyoung Sun
    • Korean Journal of Medicinal Crop Science
    • /
    • v.25 no.6
    • /
    • pp.404-410
    • /
    • 2017
  • Background: Hair loss related syndromes are increasing due to environmental pollution and stress. Hair care products are mainly prepared by mixing chemicals and natural extracts, such as those obtained from medicinal plants. The purpose of this study was to investigate the effects of 70% ethanol extracts from the flowers of Calendula officinalis, fruit body of Phellinus linteus, and the whole plant of Houttuynia cordata on the growth of CCD-986 cells, hair follicle dermal papilla cells (HFDPC), and 3T3-L1 cells. Methods and Results: All sample extracts at all concentrations, except for that from P. linteus fruit body at $500{\mu}g/m{\ell}$, were cytotoxic to CCD-986 cells. However, none of the sample extracts were cytotoxic to HFDPC. The lipid differentiation of 3T3-L1 cells regulates hair regeneration via secretion of platelet derived growth factor. The 70% ethanol extract of H. cordata whole plant promoted hair growth. Adipogenesis rate significantly increased in a treatment concentration-dependent manner. Conclusions: These results suggest that 70% ethanol extracts of C. officinalis flower, P. linteus fruit body and H. cordata could be used for the development of hair care products.

Protective Effect of Resveratrol on the Oxidative Stress-Induced Inhibition of Gap Junctional Intercellular Communication in HaCaT Keratinocytes

  • Lee, Jong-Chan;Lee, Sun-Mee;Kim, Ji-Hyun;Ahn, Soo-Mi;Lee, Byeong-Gon;Chang, Ih-Seoup
    • Biomolecules & Therapeutics
    • /
    • v.11 no.4
    • /
    • pp.224-231
    • /
    • 2003
  • The aim of this study was to investigate the effect of resveratrol on the oxidative stress-induced inhibition of gap junctional intercellular communication in HaCaT keratinocytes. Anti-oxidative activity of resveratrol was measured by $\alpha,\alpha$-diphenyl-$\beta$-picrylhydrazyl assay and dichlorodihydrofluorescein diacetate oxidation assay. Gap junctional intercellular communication in HaCaT keratinocytes was assessed using the scrape loading/dye transfer technique. Western blots and reverse transcription-polymerase chain reaction were also analyzed for connexin 43 protein and mRNA expression, respectively. Resveratrol scavenged directly the stable $\alpha,\alpha$-diphenyl-$\beta$-picrylhydrazyl radical over a concentration range of 4 mg/ml ($78.2{\pm}2.7$% of control) to 500 mg/ml ($29.9{\pm}4.2$% of control) and decreased the intracellular reactive oxygen species induced by ultraviolet A (UVA) irradiation ($89.3{\pm}1.1$% of UVA group), ultraviolet B (UVB) irradiation ($70.9{\pm}1.7$% of UVB group) and 12-0-tet-radecanoylphorbol-13-acetate (TPA, $48.3{\pm}1.1$% of TPA group), respectively. UVA irradiation and TPA markedly reduced gap junctional intercellular communication, which was restored by resveratrol. There were no significant differences in the level of connexin 43 protein and mRNA expression among any of the experimental groups. Our data suggests that resveratrol has the protective effect on the oxidative stress-induced inhibition of gap junctional intercellular communication in HaCaT keratinocytes, and this protection is likely due to the scavenging of reactive oxygen species.

Siderophore-producing rhizobacteria reduce heavy metal-induced oxidative stress in Panax ginseng Meyer

  • Huo, Yue;Kang, Jong Pyo;Ahn, Jong Chan;Kim, Yeon Ju;Piao, Chun Hong;Yang, Dong Uk;Yang, Deok Chun
    • Journal of Ginseng Research
    • /
    • v.45 no.2
    • /
    • pp.218-227
    • /
    • 2021
  • Background: Panax ginseng is one of the most important medicinal plants and is usually harvested after 5 to 6 years of cultivation in Korea. Heavy metal (HM) exposure is a type of abiotic stress that can induce oxidative stress and decrease the quality of the ginseng crop. Siderophore-producing rhizobacteria (SPR) may be capable of bioremediating HM contamination. Methods: Several isolates from ginseng rhizosphere were evaluated by in vitro screening of their plant growth-promoting traits and HM resistance. Subsequently, in planta (pot tests) and in vitro (medium tests) were designed to investigate the SPR ability to reduce oxidative stress and enhance HM resistance in P. ginseng inoculated with the SPR candidate. Results: In vitro tests revealed that the siderophore-producing Mesorhizobium panacihumi DCY119T had higher HM resistance than the other tested isolates and was selected as the SPR candidate. In the planta experiments, 2-year-old ginseng seedlings exposed to 25 mL (500 mM) Fe solution had lower biomass and higher reactive oxygen species level than control seedlings. In contrast, seedlings treated with 108 CFU/mL DCY119T for 10 minutes had higher biomass and higher levels of antioxidant genes and nonenzymatic antioxidant chemicals than untreated seedlings. When Fe concentration in the medium was increased, DCY119T can produce siderophores and scavenge reactive oxygen species to reduce Fe toxicity in addition to providing indole-3-acetic acid to promote seedling growth, thereby conferring inoculated ginseng with HM resistance. Conclusions: It was confirmed that SPR DCY119T can potentially be used for bioremediation of HM contamination.

A comparison of ventilation effects using bag-valve-mask assist device (백-밸브-마스크 보조장치를 이용한 환기효과 비교)

  • EunYoung, Seo;ByoungGil, Yoon;GaEun, Kim;YougSeok, Kim
    • The Korean Journal of Emergency Medical Services
    • /
    • v.26 no.3
    • /
    • pp.37-46
    • /
    • 2022
  • Purpose: TThe experiment was designed to compare the efficiency of ventilation between conventional BVM ventilation and a newly devised A-BVM ventilation method with Tidal volume, total ventilation rate, average Ventilation speed, and average Ventilation volume. Methods: 40 Paramedical students who agreed to participate in the study were analyzed. Values were measured using IMB PASS after 2 minutes of Brayden Pro manikin with BVM and A-BVM ventilation. The difference in general characteristics was assessed by t-test and ANOVA and the difference in ventilation methods was analyzed by IBM SPSS. Results: A significant difference was found between the two ventilation methods in terms of tidal volume (t=-11.203, p<.001), ventilation time (t=-3.834, p<.001), and optimum ventilation probability (t=10.770, p<.001). A-BVM ventilation method, rather than BVM ventilation method, showed a value close to the appropriate amount recommended by Korean Advanced Life Support (500~600mL) in tidal volume, and higher in optimum ventilation probability. Conclusion: We could identify the a better mode of ventilation. Further studies on the efficacy of existing BVM ventilation methods as compared to device augmented BVM ventilation methods should be carried out to ensure that adequate ventilation is available to patients in clinical practice.

Growth and Fruit Characteristics of 'Cheongsoo' Grape in Different Trellis Systems ('청수' 포도의 수형에 따른 수체 생육 및 과실 특성)

  • Kim, Su Jin;Park, Seo Jun;Jung, Sung Min;Noh, Jeong Ho;Hur, Youn Young;Nam, Jong Cheol;Park, Kyo Sun
    • Horticultural Science & Technology
    • /
    • v.32 no.4
    • /
    • pp.427-433
    • /
    • 2014
  • Trellising is an important cultural practice that affects grape quality and yield. Some grape cultivars require different trellising under different climate and soil conditions. To find suitable trellis conditions for grape cultivar 'Cheongsoo', we measured growth and fruit characteristics with three different trellis systems: curtain, Geneva double curtain (GDC), and modified T. The maximum light exposure of clusters in the curtain, GDC, modified T trellis systems was 670, 1,654, and $1,649{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, respectively. However, there was no difference in air temperature among the three trellis systems. Net $CO_2$ assimilation rate at $1,500{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ light intensity was 13.4, 13.7, and $8.7{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ in curtain, GDC, and modified T trellis systems, respectively. Trunk cross section area (TCSA) and bud burst rate were not significantly different among the three systems. Shoot number was 31.3, 47.0, and 37.0 in curtain, GDC, and modified T trellis systems, respectively. The shoot length was higher (243.9 cm) in the modified T trellis system than in the single curtain (171.1 cm) and GDC (151.5 cm) systems. Interior leaf number and leaf layer number were higher in the GDC system, in which there are two primary branches, in comparison to the modified T and curtain systems, which utilize one primary branch. Primary leaf area and lateral leaf area were significantly higher in the modified T trellis system in comparison to the GDC system. Berry weight, length and diameter, and total soluble solids were not significantly different among the three trellis systems. However, cluster weight and cluster number per tree were significantly higher in GDC. Titratable acidity was significantly lower in GDC. Collectively, our data suggest that the GDC trellis system is preferable for grape 'Cheongsoo' to maintain fruit quality and quantity in Korea.

Inhibition of Pancreatic Lipase Activity and Adipocyte Differentiation in 3T3-L1 Cells Treated with Purple Corn Husk and Cob Extracts (자색옥수수 포엽과 속대 추출물의 리파아제 저해활성 및 3T3-L1 지방전구세포에서의 지방분화 억제효과)

  • Lee, Ki Yeon;Hong, Soo Young;Kim, Tae Hee;Kim, Jai Eun;Park, A-Reum;Noh, Hee Sun;Kim, Si Chang;Park, Jong Yeol;Ahn, Mun Seob;Jeong, Won Jin;Kim, Hee Yeon
    • Journal of Food Hygiene and Safety
    • /
    • v.33 no.2
    • /
    • pp.131-139
    • /
    • 2018
  • Our review begins with the maize hybrid for grain, called 'Seakso 1,' which was developed in 2008 by the Gangwon Agricultural Research and Extension Services in Korea, and subsequently registered in 2011. In this study, we aimed to investigate the lipid metabolic enzyme activity and inhibitory effect on the adipocyte differentiation, in 3T3-L1 cells of the identified Seakso 1 corn husk and cob extracts (EHCS). We investigated the pancreatic lipase inhibitory effect and anti-adipogenic effect of EHCS.The lipid accumulation and adipocyte differentiation were measured by the procedure of Oil Red O staining, Real-time PCR and the Western blot analysis. The pancreatic lipase inhibitory activity of EHCS was measured at higher levels than those of the positive control (orlistat) at 100, 500, and $1,000{\mu}g/mL$. In particular, EHCS was noted as being significantly inhibited and including a measured adipocyte differentiation and lipid accumulation, when treated during the adipocyte differentiation process in 3T3-L1 cells. Based on the Oil Red O staining, EHCS inhibited lipid accumulation at 19.19%, 33.30% at $1000{\mu}g/mL$, $2000{\mu}g/mL$, respectively. The real-time PCR and Western blot analysis showed that EHCS significantly decreased in the mRNA expression and protein level of obesity-related factors, such as peroxisome-proliferatorsactivated-receptor-${\gamma}$ ($PPAR{\gamma}$) and CCAAT enhancer-binding-proteins ${\alpha}$ ($C/EBP{\alpha}$). This study potentially suggests that the Saekso 1 corn husk and cob extracts may improve lipid metabolism and reduce lipid accumulation.

Relationship of the Experience of Violence to Burnout and Job Satisfaction in Emergency Department Nurses (응급실 간호사의 폭력경험, 소진 및 직무만족도간의 관련성)

  • Sung, Mi Hae
    • Journal of Korean Clinical Nursing Research
    • /
    • v.14 no.2
    • /
    • pp.83-92
    • /
    • 2008
  • Purpose: This study was done to identify the relationship between experiences of violence, and burnout and job satisfaction of emergency department (ED) nurses and to provide basic information to improve quality of life for nurses and quality of nursing care by increasing the ability of nurses to deal with violent situations and thus, improve job satisfaction. Method: Participants (n=153) were recruited in S and B cities from October 16 to December 22, 2007. Descriptive statistics, t-test, ANOVA, and Pearson correlation coefficient were used to analyze the data. Results: Level for ED nurse burnout was 2.54 out of a possible 4, and for ED nurse job satisfaction, 2.81 out of a possible 5. There were significant differences according to sex (t=-3.217, p=.002), total clinical career (F=5.805, p<.05), and clinical career in the ED (F=1.640, p<.01). There was a significant positive correlation between violence experience and burnout (r=.500, p<.001), and significant negative correlations between violence experience and job satisfaction (r=-.300, p<.001), and between burnout and job satisfaction (r=-.492, p<.001). Conclusion: The findings from this study indicate that experiences of violence in the ED decrease work efficiency of the nurses and increase burnout. So, it is important to reduce occurrences of violence in ED and to prepare programs for dealing with violence and providing emotional support in cases of violence directed at nurses.

  • PDF

A Study on the Effect of Packaging Design Considering SCM Aspects on Logistics Efficiency (Focusing on the case of domestic A company)

  • Jung, Sung-Tae
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
    • /
    • v.26 no.1
    • /
    • pp.11-17
    • /
    • 2020
  • This study conducted a case study and a questionnaire analysis in parallel. In the case study, a study was conducted on domestic manufacturer A by analyzing pallet loading efficiency of RRP(Retail Ready Packaging) products and pallet loading efficiency of MWC(Membership Wholesale Club) delivered products. As a result of the pallet loading efficiency simulation of 50 RRP products of Manufacturer A, it was 80.0% based on the T-11 type pallet and 84.3% based on the T-12 type pallet. It was found It refers that the route of producing the product from the manufacturer A and delivering it to the MWC A in the form of RRP resulted in the decrease of the pallet loading efficiency through the change of the loading pattern and the adjustment of the number of loads. As a result of analyzing the questionnaire about whether the overall efficiency of the supply chain will be improved if the operation of the packaging system considering the SCM(Supply Chain Management) aspect is χ2 = 178.500, there was a statistically significant difference at the significance level of 0.000. Manufacturers and logistics companies answered "yes" the most, but distributors answered "is average" the most, confirming that the packaging can be constructed with the highest operational efficiency. Therefore, as a result of confirming the impact of packaging design considering the SCM aspect on logistics efficiency, it indicates the importance of closer collaboration between manufacturers and distributors.

Near Infrared Spectroscopy for Measuring Soil Properties

  • Ryu, Kwan-Shig;Kim, Bok-Jin;Park, Woo-Churl;Cho, Rae-Kwang
    • Near Infrared Analysis
    • /
    • v.1 no.1
    • /
    • pp.37-41
    • /
    • 2000
  • The purpose of this research was to develop a the reflection technique with near infrared (NIR) radiation for estimating soil components. NIR reflectance was scanned at 2nm intervals from 1100 to 2500nm with an InfraAlyzer 500 (Bran & Luebbe Co.). Over 400 soil sample from fields of different crops and land-use over Youngnam and Honam regions were used to obtain mean diffuse reflection of the soil for the calibration and validation of the calibration set in estimating moisture, organic matter (OM) and total nitrogen (T-N) of the soils. Multiple linear regression (MLR) was used to evaluate the correlation of NIR spectroscopy method. Reflection pattern of NIR spectra for finely sized sample (<0.5mm) and coarsely sized soil(<2mm) did not show much difference. The results showed that NIR spectroscopy and coarsely sized soil (<2mm) did not show much difference. The results showed that NIR spectroscopy could be used as a routine soil testing method in estimating OM, moisture, T-N in soil samples simultaneously.