Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
권호 표지
DOI QR코드

DOI QR Code

Inhibition of Pancreatic Lipase Activity and Adipocyte Differentiation in 3T3-L1 Cells Treated with Purple Corn Husk and Cob Extracts

자색옥수수 포엽과 속대 추출물의 리파아제 저해활성 및 3T3-L1 지방전구세포에서의 지방분화 억제효과

  • Lee, Ki Yeon (Agriproduct Processing Experiment Station, Gangwon-do Agricultural Research and Experiment Services) ;
  • Hong, Soo Young (Agriproduct Processing Experiment Station, Gangwon-do Agricultural Research and Experiment Services) ;
  • Kim, Tae Hee (Agriproduct Processing Experiment Station, Gangwon-do Agricultural Research and Experiment Services) ;
  • Kim, Jai Eun (Agriproduct Processing Experiment Station, Gangwon-do Agricultural Research and Experiment Services) ;
  • Park, A-Reum (Agriproduct Processing Experiment Station, Gangwon-do Agricultural Research and Experiment Services) ;
  • Noh, Hee Sun (Agriproduct Processing Experiment Station, Gangwon-do Agricultural Research and Experiment Services) ;
  • Kim, Si Chang (Agriproduct Processing Experiment Station, Gangwon-do Agricultural Research and Experiment Services) ;
  • Park, Jong Yeol (Hongcheon Maize Experiment, Gangwon-do Agricultural Research and Experiment Services) ;
  • Ahn, Mun Seob (Agriproduct Processing Experiment Station, Gangwon-do Agricultural Research and Experiment Services) ;
  • Jeong, Won Jin (GUmediherb Co.) ;
  • Kim, Hee Yeon (Agriproduct Processing Experiment Station, Gangwon-do Agricultural Research and Experiment Services)
  • 이기연 (강원도농업기술원 농식품연구소) ;
  • 홍수영 (강원도농업기술원 농식품연구소) ;
  • 김태희 (강원도농업기술원 농식품연구소) ;
  • 김재은 (강원도농업기술원 농식품연구소) ;
  • 박아름 (강원도농업기술원 농식품연구소) ;
  • 노희선 (강원도농업기술원 농식품연구소) ;
  • 김시창 (강원도농업기술원 농식품연구소) ;
  • 박종열 (강원도농업기술원 옥수수연구소) ;
  • 안문섭 (강원도농업기술원 농식품연구소) ;
  • 정원진 (지유본초) ;
  • 김희연 (강원도농업기술원 농식품연구소)
  • Received : 2017.11.30
  • Accepted : 2018.02.02
  • Published : 2018.04.30
Download PDF
⟨ Previous Next ⟩

Abstract

Our review begins with the maize hybrid for grain, called 'Seakso 1,' which was developed in 2008 by the Gangwon Agricultural Research and Extension Services in Korea, and subsequently registered in 2011. In this study, we aimed to investigate the lipid metabolic enzyme activity and inhibitory effect on the adipocyte differentiation, in 3T3-L1 cells of the identified Seakso 1 corn husk and cob extracts (EHCS). We investigated the pancreatic lipase inhibitory effect and anti-adipogenic effect of EHCS.The lipid accumulation and adipocyte differentiation were measured by the procedure of Oil Red O staining, Real-time PCR and the Western blot analysis. The pancreatic lipase inhibitory activity of EHCS was measured at higher levels than those of the positive control (orlistat) at 100, 500, and $1,000{\mu}g/mL$. In particular, EHCS was noted as being significantly inhibited and including a measured adipocyte differentiation and lipid accumulation, when treated during the adipocyte differentiation process in 3T3-L1 cells. Based on the Oil Red O staining, EHCS inhibited lipid accumulation at 19.19%, 33.30% at $1000{\mu}g/mL$, $2000{\mu}g/mL$, respectively. The real-time PCR and Western blot analysis showed that EHCS significantly decreased in the mRNA expression and protein level of obesity-related factors, such as peroxisome-proliferatorsactivated-receptor-${\gamma}$ ($PPAR{\gamma}$) and CCAAT enhancer-binding-proteins ${\alpha}$ ($C/EBP{\alpha}$). This study potentially suggests that the Saekso 1 corn husk and cob extracts may improve lipid metabolism and reduce lipid accumulation.

본 연구는 자색옥수수 색소 1호 포엽과 속대 추출물의 항비만 활성을 검정하고자 지방분해효소 저해활성을 평가하고 3T3-L1 지방전구세포에서 지방분화억제 효과를 검정하고자 수행되었다. Pancreatic lipase 저해 활성 결과, 색소 1호 포엽 및 속대 추출물의 100, 500, $1,000{\mu}g/mL$ 농도처리구에서 양성대조군인 orlistat 보다 높은 저해 활성을 나타내었다. 3T3-L1 지방전구세포를 배양하여 색소 1호 포엽 및 속대 추출물의 세포독성 평가를 수행한 결과, 추출물은 모든 처리농도에서 세포 생존율에 영향을 미치지 않은 것으로 확인되었다. 분화된 3T3-L1 지방전구세포에서 색소 1호 포엽과 속대 추출물을 처리하지 않고 분화시킨 대조군은 lipid droplet의 형성이 활발하게 유발되었으나 색소 1호 포엽 및 속대 추출물의 처리에 의해 농도 의존적으로 lipid droplet의 형성이 억제되는 것으로 나타났다. Real-time PCR과 Western blot을 실시하여 $PPAR{\gamma}$와 $C/EBP{\alpha}$ 유전자 및 단백질 발현량을 측정한 결과, 추출물을 처리하지 않고 분화시킨 대조군에서는 $PPAR{\gamma}$와 $C/EBP{\alpha}$의 유전자 및 단백질 발현이 증가하였으며, 추출물 처리에 의해 $PPAR{\gamma}$와 $C/EBP{\alpha}$의 유전자 및 단백질 발현이 유의적으로 감소하였다. 본 연구 결과는 색소 1호 포엽 및 속대 추출물이 pancreatic lipase 활성 및 지방전구 세포의 분화를 억제시킴으로써 항비만 활성 기능성 물질로의 활용 가능성이 높음을 시사한다.

Keywords

References

  1. Kopelman P. G.: Obesity as a medical problem. Nature, 404(6778), 635-643 (2000). https://doi.org/10.1038/35007508
  2. Kang W. Y., Kim M. Y., Jin J. Y., Yang H. K., Hong H. J., Kim D. G., Han C. H., Lee Y. J.: Anti-obesity effects of onion juice in high fat diet-induced obese rats Korean J. Vet Res., 50(1), 1-10 (2010).
  3. Kissebah A. H., Vydelingum N., Murray R., Evans D. J., Hartz A. J., Kalkhoff R. K., Adams P. W.: Relation of body fat distribution to metabolic complications of obesity. J Clin Endocrinol Metab., 54, 254-260 (1982). https://doi.org/10.1210/jcem-54-2-254
  4. Krotkiewski M., Bjorntorp P., Sjostrom L., Smith U.: Impact of obesity on metabolism in men and women. Importance of regional adipose tissue distribution. J Clin Invest., 72, 1150-1162 (1983). https://doi.org/10.1172/JCI111040
  5. Ginsberg H. N., Le N. A., Gibson J. C.: Regulation of the production and catabolism of plasma low density lipoproteins in hypertriglyceridemic subjects. Effect of weight loss. J Clin Invest., 75, 614-623 (1985). https://doi.org/10.1172/JCI111739
  6. Jeon S. M., Bok S. H., Jang M. K., Lee M. K., Nam K. T., Park Y. B., Rhee S. J., Choi M. S.: Antioxidative activity of naringin and lovastatin in high cholesterol-fed rabbits. Life Sci., 69, 2855-2866 (2001). https://doi.org/10.1016/S0024-3205(01)01363-7
  7. Wang H., Eckel R. H.: Lipoprotein lipase: from gene to obesity. Am J Physiol Endocrinol Metab., 297, 271-288 (2009). https://doi.org/10.1152/ajpendo.90920.2008
  8. Braun J. E., Severson D. L.: Regulation of the synthesis, processing and translocation of lipoprotein lipase. Biochem. J., 287, 337-347 (1992). https://doi.org/10.1042/bj2870337
  9. Heimburger D. C., Lucas C. P., Robbins D. C., Chung J., Heymsfield S. B.: Weight control and risk factor reduction in obese subjects treated for 2 years with orlistat: a randomized controlled trial. J. Am. Med. Assoc., 281, 235-242 (1999). https://doi.org/10.1001/jama.281.3.235
  10. Choi H. Y., Kim G. H.: Inhibitory Effects of Allium senescens L. methanol extracts on reactive oxygen species production and lipid accumulation during differentiation in 3T3-L1 cells. Korea J. Food Sci. Technol., 46(4), 498-504 (2014). https://doi.org/10.9721/KJFST.2014.46.4.498
  11. Lee O. H., Kwon Y. I., Hong H. D., Park C. S., Lee B. Y., Kim Y. C.: Production of reactive oxygen species and changes in antioxidant enzyme activities during differentiation of 3T3-L1 adipocyte. J. Korean. Soc. Appl. Biol. Chem., 52, 70-75 (2009).
  12. Halliwell B.: Antioxidants in human health and disease. Annu. Rev. Nutr., 16, 33-50 (1996). https://doi.org/10.1146/annurev.nu.16.070196.000341
  13. Yamashita A., Soga Y., Iwamoto Y., Asano T., Li Y., Abiko Y., Nishimura F.: DNA microarray analyses of genes expressed differentially in 3T3-L1 adipocytes co-cultured with murine macrophage cell line RAW264.7 in the presence of the tolllike receptor 4 ligand bacterial endotoxin. Int. J. Obesity, 32, 1725-1729 (2008). https://doi.org/10.1038/ijo.2008.153
  14. Moreno D. A., Ilic N., Poulev A., Brasaemle D. L., Fried S. K., Raskin I.: Inhibitory effects of grape seed extract on lipases. Nutrition, 19, 876-879 (2003). https://doi.org/10.1016/S0899-9007(03)00167-9
  15. Ntambi J. M., Kim Y. C.: Adipocyte differentiation and gene expression. J. Nutr., 130, 3122-3126 (2000). https://doi.org/10.1093/jn/130.12.3122S
  16. Davidson M. H,. Hauptman J., DiGirolamo M., Foreyt J. P., Halsted C. H., Heber D., Heimburger D. C., Lucas C. P., Robbins D. C., Chung J., Heymsfield S. B.: Weight control and risk factor reduction in obese subjects treated for 2 years with orlistat: a randomized controlled trial. J. Am. Med. Assoc., 281, 235-242 (1999). https://doi.org/10.1001/jama.281.3.235
  17. Yao F., MacKenzie R. G.: Obesity drug update: The lost decade? Pharmaceuticals, 3, 3494-3521 (2010). https://doi.org/10.3390/ph3123494
  18. Lee M. R., Oh D. S., Wee A. J., Yun B. S., Jang S. A., Sung C. K.; Anti-obesity effects of Lentinus edodes on obese mice induced by high fat diet. J Korean Soc. Food Sci. Nutr., 43(2), 194-199 (2014). https://doi.org/10.3746/jkfn.2014.43.2.194
  19. Li C. Y., Kim H. W., Won S. R., Min H. K., Park K. J., Park J. Y., Ahn M. S., Rhee H. I.: Corn husk as a potential source of anthocyanins. J. Agric. Food Chem., 56, 11413-11416 (2008). https://doi.org/10.1021/jf802201c
  20. Kim S. L., Kim E. H., Son Y. K., Song J. C., Hwang J. J., Hur H. S.: Identification of anthocyanin pigments in black waxy corn kernels. Korean J. Breed, 31, 408-415 (1999).
  21. Kim S. L., Hwang J. J., Song J., Song J. C., Jung K. H.: Extraction, purification and quantification of anthocyanins in colored rice, black soy bean and black xaxy corn. Korean J. Breed, 32, 146-152 (2000).
  22. Lee K. Y., Kim T. H., Lim S. H., Park J. Y., Kim K. H., Ahn M. S., Kim H. Y.: Proximate, free sugar, fatty acids composition and anthocyanins of Saekso 2 corn kernels J. Food Hyg. Saf., 31(5), 335-341 (2016). https://doi.org/10.13103/JFHS.2016.31.5.335
  23. Lee K. Y., Kim J. E., Hong S. Y., Kim T. H., Noh H. S., Kim S. C., Park J. Y., Ahn M. S., Kim H. Y.; Effect of Saekso 2 corn kernels and cobs extracts on antioxidant activity in rats fed high fat-cholesterol diet. J. Food Hyg Saf., 31(6), 399-405 (2016). https://doi.org/10.13103/JFHS.2016.31.6.399
  24. Kim J. T., Son Y. B., Lee J. S., Baek S. B., Woo K. S., Jung G. H., Kim M. J., Jeong K. H., Kwon. Y. U.: Effects of particle size on antioxidant activity and cytotoxicity in purple corn seed powder. Korean J. Crop. Sci., 57, 353-358 (2012). https://doi.org/10.7740/kjcs.2012.57.4.353
  25. Lee J. S., Son B. M., Kim J. T., Ku J. H., Han O. K., Baek S. B., Moon J. K., Hwang J. J., Kwon Y. U.: Change of total anthocyanin contents and antioxidant activities of purple waxy corn inbred lines and hybrids during grain filling. Korean J. Breed Sci., 44, 290-300 (2012).
  26. Duan X. W., Jiang Y. M., Zhang Z. Q., Shi J.: Antioxidant properties of anthocyanin extracted from litchi (Litchi chinenesis Sonn.) fruit pericarp tissues in relation to their role in the pericarp browning. Food Chem., 101, 1365-1371 (2006).
  27. Chung M. G., Lim J. D.: Antioxidant, Anticancer and Immune Activation of Anthocyanin Fraction from Rubus coreanus Miquel fruits (Bokbunja). Korean J. Medicinal Crop Sci., 20, 259-269 (2012). https://doi.org/10.7783/KJMCS.2012.20.4.259
  28. Kim J. H., Kim H. J., Kim C. Y., Jung H. Y., Kim Y. O., Ju J. Y., Shin C. S.: Development of lipase inhibitors from various derivatives of monascus pigment produced by Monascus fermentation. Food Chem., 101, 357-364 (2009).
  29. Lim C. L., Son H. J., Cho I. Y., Kim G. W., Choi S. J., I. S., Han K. Y., Choi J. Y., Noh B. S.: Physiological functionality and cytotoxic effect of Korean traditional noble wine, Samhaeju, and commercial rice wine on various tumor cell lines. Korean J. Food Sci. Technol., 41(6), 687-693 (2009).
  30. Choi J. H., Park Y. H., Lee I. S., Lee S. P., Yu M. H.: Antioxidant activity and inhibitory effect of Aster scaber Thunb. extract on adipocyte differentiation in 3T3-L1 cells. Korean J. Food Sci. Technol., 45(3), 356-363 (2013). https://doi.org/10.9721/KJFST.2013.45.3.356
  31. Lim H. J., Seo J. E., Chang Y. H., Han B. K., Jeong J. K., Park S. B., Choi H. J., Whang J. N.: Anti-obesity effects of Jeju Hallabong Tangor (Citrus kiyomi x ponkan) Peel extracts in 3T3-L1 adipocytes. J. Korean Soc. Food Sci. Nutr., 43(11), 1688-1694 (2014). https://doi.org/10.3746/jkfn.2014.43.11.1688
  32. Fuleki, T., Francis F. J.: Quantitative methods for anthocyanins. 1. Extraction and determination of total anthocyanin in cranberries. J. Food Sci., 33, 72-77 (1968). https://doi.org/10.1111/j.1365-2621.1968.tb00887.x
  33. Lee J. W., Lee H. H., Rhim J. W., Jo J. S.: Determination of the conditions for anthocyanin extraction from purplefleshed sweet potato. J. Korean Soc. Food Sci. Nutr., 29, 790-795 (2000).
  34. Rhee H. I.: Development of high anthocyanin corn cultivar. Rural Development Administraton Report, Jeonju, Korea, pp. 20-49 (2008).
  35. Pascual-Teresa S., Santos-Buelga C., Rivas-Gonzalo J. C.: LC-MS analysis of anthocyanins from purple corn cob. J. Sci. Food Agric., 82, 1003-1006 (2002). https://doi.org/10.1002/jsfa.1143
  36. Ahn H. C., Jhung R. N., Choe E. O.: In vitro Antioxidant Activity and ${\alpha}$-Glucosidase and Pancreatic Lipase Inhibitory Activities of Several Korean Sanchae. Korean J. Food Sci. Technol., 47(2), 164-169 (2015). https://doi.org/10.9721/KJFST.2015.47.2.164
  37. Kim Y. J., Kim B. H., Lee S. Y., Kim M. S., Park C. S., Rhee M. S., Lee K. H., Kim D. S.: Screening of medicinal plants for development of functional food ingredients with antiobesity. J. Korean Soc. Appl. Biol. Chem., 49, 221-226 (2006).
  38. Choi J. H., Park Y. H., Lee I. S., Lee S. P., Yu M. H.: Antioxidant activity and inhibitory effect of Aster scaber Thunb. extract on adipocyte differentiation in 3T3-L1 cells. Korean J. Food Sci. Technol., 45(3), 356-363 (2013). https://doi.org/10.9721/KJFST.2013.45.3.356
  39. Cornelius P, MacDougald O. A., Lane M. D., Regulation of adipocyte development. Annu. Rev. Nutr., 14, 99-129 (1994). https://doi.org/10.1146/annurev.nu.14.070194.000531
  40. Rosen E. D., Spiegelman B. M.: Molecular regulation of adipogenesis. Annu. Rev. Cell Dev. Bi., 16, 145-171 (2000). https://doi.org/10.1146/annurev.cellbio.16.1.145
  41. Wang N. D., Finegold M. J., Bradley A., Ou C. N., Abdelsayed S. V., Wilde M. D., Taylor L. R., Wilson D. R., Darlington G. J.: Impaired energy homeostasis in C/EBP alpha knockout mice. Science, 269, 1108-1112 (1995). https://doi.org/10.1126/science.7652557
  42. Lee C. W., Park Y. I., Kim S. H., Lim H. K., Chung M. J.: Antioxidant and anti-Adipogenic activities of bread containing corn silk, Job's tears, Lentinus edodes, and apple peel in 3T3-L1 preadipocytes. J. Korean Soc. Food Sci. Nutr., 45(5), 651-663 (2016). https://doi.org/10.3746/jkfn.2016.45.5.651
  43. Morrison R. F., Farmer S. R.: Hormonal signaling and transcriptional control of adipocyte differntiation. J. Nutr. 130, 3116S-3121S (2000). https://doi.org/10.1093/jn/130.12.3116S

Cited by

  1. Assessment of Nutritional Components, Antioxidant Contents and Physiological Activity of Purple Corn Husk and Cob Extracts vol.33, pp.6, 2018, https://doi.org/10.13103/JFHS.2018.33.6.500