• Microbiology and Biotechnology Letters
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• v.32 no.3
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• pp.206-211
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• 2004

Levansucrase gene(lscA) from Pseudomonas aurantiaca was subcloned downstream of GAL1 promoter in pYES 2.0 and pYInu-AT [GAL10 promoter ＋ exoinulinase signal sequence of Kluyveromyces marxianus], resulting pYES-lscA and p YInu-lscA, respectively. The two expression plasmids were introduced into an invertase-deficient strain, Sacchromayces cerevisiae SEY2102, and transformants with high activity of levansucrase were selected. When each yeast transform ants was cultivated in medium containing galactose, the extracellular and intracellular activities of levansucrase reached about 8.62 U/ml with the strain harboring pYES-lscA and 5.43 U/ml with the strain harboring pYInu-lscA. The levansucrase activity of 80% was detected in the periplasmic space and cytoplasm. The levansucrase activity in the medium of SEY2102/pYInu-lscA was 0.87 U/ml whereas that of SEY2102/pYES-lscA was 0.47 U/ml, which implying the exoinulinase signal sequence didn't enhance the secretion efficiency of levansucrase. Furthermore, the recombinant levansucrase expressed in yeast seems to be produced as a hyper-glycosylated form.

• Korean Journal of Metals and Materials
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• v.46 no.3
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• pp.118-124
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• 2008

Abstract: A minimum commitment method(MCM) was applied to predict the long-term creep rupture life for type 316LN stainless steel(SS). Lots of the creep-rupture data for the type 316LN SS were collected through world-wide literature surveys and the experimental data of KAERI. Using these data, the long-term creep rupture life above ${10}^5$ hour was predicted by means of the MCM. In order to obtain the most appropriate value for the constant A being used in the MCM equation, trial and error method was used for the wide ranges from -0.12 to 0.12, and the best value was determined by using the coefficient of determination, $R^2$ which is a statistical parameter. A suitable value for the A in type 316LN stainless steel was found to be at -0.02 ~ -0.05 ranges. It is considered that the MCM will be superior in creep-life prediction to commonly-used timetemperature parametric method, because the P(T) and G($\sigma$) functions are determined from the regression method based on experimental data.

• Journal of Korean Society of Environmental Engineers
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• v.32 no.5
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• pp.477-486
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• 2010

This study was performed with the object in which it improves the nitrification by using RBC, that is one of the biological waste water treatment process. By applying the Modified Dephanox process to RBC in this research in order to evaluate the improvement of the nitrification by RBC a research was conducted. There is the most conspicuous feature of the process of using RBC. it is that the nitrification can be smoothly performed even if the suspended solid of the high concentration as the interference factor in the nitrification tank is flowed in. Moreover, as a result of experiment, TCOD removal efficiency of the process showed up more than about 90%. when influent TCOD loading rate was 0.04~0.1 kg / $day{\cdot}m^3$. and T-N removal efficiency is high at about 75% in spite of the process operating of the laboratory scale was observed. Also, As increasing influent ${PO_4}^{3-}$-P, T-P loading rate, ${PO_4}^{3-}$-P, T-P removal efficiency was increased. Finally, it was elucidated that the utilization of RBC in external nitrification system resulted in not only high nitrification performance but also stable system operation by minimizing inhibitory effect of overflowed suspended solid (SS).

• KCID journal
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• v.14 no.2
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• pp.214-224
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• 2007

This study was carried out to investigate of water quality and irrigation water use possibility of reservoirs near Saemangeum for upland and horticultural fields. Water samples were taken at 6 reservoirs for 5 months from June, 2006 to November, 2006. The water temperature, pH, EC, EC, chlorophyll - a of 6 reservoirs were ranged 8.7-$31.2^circC$, 6.9-9.2, 73.0-637.0$\mu$S/cm, 0.9-443.2mg/$m^3$, respectively. The concentration of DO, BOD, COD, T-N, T-P and SS were ranged 5.7-11.7mg/L, 0.5-8.9mg/L, 2.9-18.0mg/L, 0.07-6.52mg/L, 0.002-0.406mg/L, 0.5-54.0mg/L Also, storage ratio and storage capacity of Mije reservoir, Okgu reservoir, Oknyeo reservoir, Neungje reservoir were decreased between June and April, but those of Oksan reservoir was kept high during irrigation period. Water supply of reservoirs was 4,474,100$m^3$(Oksan), 6,165,900$m^3$(Mije), 13,209,900$m^3$(Okgu), 4,675,600$m^3$(Oknyeo), 7,682,000$m^3$(Neungje), 19,231,000$m^3$(Cheongho) in 2006, respectively. It is resevoirs for upland and horticultural fields that use main irrigation water resources before Saemanguem fresh-water lake development, and use assistance irrigation water resources in emergency after Saemanguem fresh-water lake development. In the meantime, for continuous use of reservoir as irrigation water resource for upland and horticultural fields, we must examine about surplus water capacity, and need investigation about supply possibility of irrigation water, condition of irrigation water, water quality.

• Korean Journal of Environmental Biology
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• v.31 no.1
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• pp.37-44
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• 2013

This study was to investigate water quality and biotic community characteristics in Juam Lake. In water quality, water temperatures was $3.8{\sim}21.2^{\circ}C$, 6.7~8.6 in pH, $64{\sim}76{\mu}s\;cm^{-1}$ in Conductivity, $5.3{\sim}13.2mg\;L^{-1}$ in DO, $2.5{\sim}3.3mg\;L^{-1}$ in COD, $1.0{\sim}5.1mg\;L^{-1}$ in SS, $0.622{\sim}0.841mg\;L^{-1}$ in T-N, $0.007{\sim}0.019mg\;L^{-1}$ in T-P and $2.8{\sim}8.8mg\;m^{-3}$ in Chl-a. Revised Carlson's Index (TSIm) assessment using total phosphorus (TP) and chlorophyll-a domonstrated that the trophic states of Juam Lake were rated as mesotrophic. A total of 53 species of phytoplankton were identified. They were 28 Bacillariophyceae, 13 Chlorophyceae, 3 Cyanophyceae, and 9 Other algal taxa. The standing crops of phytoplankton was ranged from $113cells\;mL^{-1}$ to $2,909cells\;mL^{-1}$. A total of 16 species of zooplankton were identified (10 rotifers, 4 cladocerans and 2 copepods). Total zooplankton abundance was $309ind.\;L^{-1}$ to $435ind.\;L^{-1}$. The collected benthic macroinvertebrates from the surveyed sites in Juam Lake were 1,038 individuals, 33 species, 21 families and 12 orders. A dominant species was Uracanthella rufa and a subdominant species was Ecdyonurus kibunensis. Hydrophytes recorded from Juam Lake were identified 9 taxa. Emerged plants, floating plants among the hydrophytes was classified 8, 1 taxa, respectively. Ecosystem disturbance wildplant by Environment Ministry found were Paspalum distichum var. distichum and Ambrosia artemisiaefolia. A total of 30 species (6 families) were collected fishs from Juam Lake. There were 10 Korean endemic species (33.3% of collected species number) and 3 exotic species (10.0%).

• Journal of Environmental Science International
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• v.5 no.6
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• pp.763-770
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• 1996

This study was performed to analyze the variation characteristics of writer qulity, correlation analysis of water quality data at each site and among the items of water Quality data. Water quality for analysis was monthly values of water temperature, pH, dissolved oxygen, biochemical oxygen demand, chemical oxygen demand, suspended solid, 7-N and T-P checked in Daecheong Lake from January to December, 1995. It was analyzed variation of monthly water qulity was well from February to April, water temperature and COD seemed to have high correlationships at all sites. Regression equation is COD = 0.07 Water temperature +1.23 ($R^2$: 0.7616) . Results of the correlation analysis of water quality data showed that DO had high correlationships between site 1 and site 2, BOD did site 1 and 3, COD did site 1 and 2, 55 did site 5 and 6, 7-N did 2 and 3, 7-P did site 4 and 6. Regression equations for estimate of water quality data are as follows. $DO_1$=4.46+0.59 DO, ($R^2$=0.8868), $BOD_1$ = 0, 52+0.63 BOD3 ($R^2$ = 0.6390) $COD_2$ = 0.44+0.71 $COD_1$ ($R^2$ = 0.9183), SS6 = 0.89+0.7055.($R^2$ = 0.9155) $TN_3$ = 0.151 +0.886 $TN_2$ ($R^2$ = 0.9415), $TP_4$ = 0.004+5.758 $TP_6$ ($R^2$ = 0.9669)

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.4
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• pp.543-550
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• 2014

Fat and fatty acids in muscle and adipose tissues are among the major factors influencing meat quality particularly nutritional value and palatability. The present study was carried out to examine the effects of supplementing inorganic selenium (Se), iodine (I) and a combination of both on fatty acid compositions in serum, and supraspinatus (SS), longissimus lumborum (LL), and semitendinosus (ST) muscles in goats. Twenty-four, 7 to 8 months old, Kacang male goats with a mean live weight of $22.00{\pm}1.17kg$ were individually and randomly assigned into four groups of six animals each for 100 d of feeding prior to slaughter. The animals were offered the same concentrate (basal) diet as 1% of body weight with ad libitum amount of fresh guinea grass. The four groups were as follows: T1 (control) - basal diet without supplementation; T2 - basal diet with 0.6 mg Se/kg DM; T3 - basal diet with 0.6 mg I/kg DM; T4 - basal diet with combination of 0.6 mg Se/kg DM and 0.6 mg I/kg DM. The major fatty acids (FAs) detected in the serum were palmitic (C16:0), stearic (C18:0), oleic (C18:1n9) and linoleic (C18:2n-6), while the major FAs in the selected muscles were C16:0, C18:0 and C18:1n9 acids. The main polyunsaturated fatty acids (PUFA) detected in muscles and serum were (CI8:2n-6), linolenic acid (C18:3n-3), and arachidonic acid (C20:4n-6). No significant differences (p>0.05) were observed in the concentration of total saturated fatty acids (SFA) among the four groups. PUFA concentrations in the goats supplemented with Se (T2) were significantly higher (p<0.05) than the goats of the control group (T1). The PUFA: SFA ratio was significantly higher in the animals supplemented with dietary Se (T2) than those of control ones (T1). It is concluded that dietary supplementation of inorganic Se increased the unsaturated fatty acids in muscle. The supplementation of iodine with or without Se had negligible effects on muscle fatty acid content of Kacang crossbred male goats.

• Journal of the Korean Society for Marine Environment & Energy
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• v.13 no.4
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• pp.223-233
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• 2010

Spatio-temporal variations of long-term water qualities (COD, SS, $Chl-{\alpha}$, N-related nutrients (TN, TDN, $NO_3^-$, $NH_4^+$), P-related nutrients (TP, TDP, $PO_4^{3-}$)) at two stations (St. SD, St. GG) in the Geumgang Reservoir were investigated from August 2001 to July 2008. Statistical methods such as t-test, factor analysis, and multi-regression analysis were applied to the water quality data in the reservoir as well as mass balances on TP. From the temporal comparisons of the water qualities between 2002 and 2007, average concentrations of $NH_4^+$, $PO_4^{3-}$, and TDP gradually decreased down by 60%, 24%, 52% in 2007. However, those of TP and $Chl-{\alpha}$ increased to 99% and 423% during the period. From the spatial comparisons between the two stations, St. GG showed higher concentrations for all of the N- and P-related nutrients than in St. SD, while opposite result for the $Chl-{\alpha}$. The factor analysis showed that "the seasonal variations of N- and P-related nutrients" were the two dominant factors occupying 49% of total variances of water qualities. Based on this result, multi-regression analysis executed for the two most influential parameters (TP and $Chl-{\alpha}$) focusing on the seasonal variations of these parameters: SS and $Chl-{\alpha}$ has contributed decisively to the concentrations of TP during the wet and dry season, respectively. On the other hand, COD and TP has been important for the $Chl-{\alpha}$ during the wet and dry season, respectively. From the established mass balances of TP loadings in the Geumgang Reservoir, Other Sources (60%) occupied the greatest contribution and Fluvial Input (38%) and Sediment (1%) during the wet season. However, both Fluvial Water (48%) and Other Sources (47%) supplied comparable amount of inputs and Sediment (5%) showed significantly increased input during the dry seasons. Recently especially during the dry winter seasons, migrating bird's excretion was estimated to contribute up to 8% of total TP input and 21% of Other Sources.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.16 no.1
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• pp.181-191
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• 2013

The design process of ecological and environmental detention system located in the housing district of Sinjeong 3-jigu in Seoul are as follows. At stage one, a new dispersal detention was created in the neighborhood park located near the originally planned detention. From this, the amount of storage of this dispersal detention system was enlarged from $28,337m^3/d$, the initial storage amount, to $33,606m^3/d$ as the post storage amount, responsible to the amount of rainfall which happens every 100 years. In particular, the SSB (Sustainable Structured wetland Biotop) system, which was the New Excellent Technology verified by the Ministry of Environment (No. 258) was applied to enhance ecological functioning and water quality with the detention as a constructed wetland. At stage two, the treatment plans for non-point pollutant source occurred at the initial period of rain, flowing into the detention system were built for purifying the water of the retention pond at the base of the detentions, and the water-circulation system was designed at the dispersal detentions on the period of regular rainfalls. The non-point pollutant source flowing into detention site was calculated as $11,699m^3/d$ flowing down from seven small watersheds, which occurred at the initial period of rain. In particular the SSB systems improved the average efficiency of the water processing performance to BOD 60%, SS 90%, T-N 30%, T-P 60%. At stage three, the ecological network and biological diversity were strongly considered so that it brought the residents with amenity places. In particular, the dispersal detentions were successfully designed to restore the ecological habitat of endangered plant and animal species such as narrow-mouthed.

• Proceedings of the Korea Water Resources Association Conference
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• 2011.05a
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• pp.283-287
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• 2011

농업비점오염원의 오염원 관리를 위해서는 오염물질의 발생과 운반에 직접적으로 영향을 미치는 농업수문학 기술에 바탕을 두고 우리나라의 집약농업의 특성을 정확히 반영할 수 있는 최적관리방법의 개발과 비점오염저감효과를 검증할 수 있는 현장실험이 수반되어야 한다. 따라서 본 연구에서는 지표피복재를 이용하여 밭의 토양침투를 증가시고 유속 및 유출량 감소를 통해 토양유실과 비점오염원을 저감하고자 하였다. 실험방법은 실제 영농과 동일하게 무를 재배한 뒤 볏짚거적을 이용하여 밭의 토양 표면을 피복하여 유량과 수질농도를 측정하였다. 연구기간 동안 총 3차례(2010년 9월 9~12일, 9월 21일, 10월 2~3일)의 강우가 발생하였고, 이때 발생한 강우량은 각 359.2 mm, 49 mm, 28.8 mm이였다. 각 시험포에서 볏짚거적으로 인해 저감된 유출량의 저감효율은 볏짚거적을 피복하지 않은 나지상태의 밭 보다 약 33∼75%의 저감효과가 있는 것으로 나타났다. 75%의 유출률 저감 효과가 가장 크게 나타난 강우사상은 30 mm 이하의 강우가 발생한 10월 2~3일의 강우사상에서 나타났다. 강우량과 강우강도는 28.8 mm와 1.92 mm/hr으로 나타났으며, 이때 발생한 유출량은 나지에서 $0.36\;m^3$, 볏짚거적을 피복한 시험포에서는 $0.08\;m^3$이 발생하였다. 나지상태와 볏짚거적을 피복한 시험포의 오염부하의 값을 비교한 결과 볏짚거적을 피복한 시험포에서 $BOD_5$ 항목은 64.3%, SS 80.8%, $COD_{Mn}$ 66.7%, DOC 80.2%, T-N 56.6% 그리고 T-P 56.1%의 오염부하 저감효과가 나타났다. 본 연구의 결과와 같이 지표를 볏짚거적으로 피복할 경우 시험포에서 유출시작 시간 지연효과와 강우에 의한 첨두유출량 및 유출량 감소 효과가 나타나는 것으로 나타났으며, 연구결과는 농업 비점오염원의 최적관리를 위한 과학적 근거자료 제공 및 기초자료로 활용할 수 있을 것으로 판단된다.