• 생명과학회지
• /
• 제12권1호
• /
• pp.67-76
• /
• 2002

Agrobacterium(LBA4404/pBI1121)을 이용하여 형질전환된 애기장대 (Arabidopsis thaliana)를 대상으로 T2, T3, F3세대에서의 도입된 외래 유전자의 비활성화 현상을 조사하였다. Kanamaycin저항성 개체들의 GUS유전자 발현을 분석한 결과, T2세대에서 조사된 12계통 중 5계통에서 GUS 비활성 개체가 관찰되었다 (GUS유전자 비활성율 2.3%). Multi copy T-DNA 계통을 조사한 결과, GUS 비활성 정도가 더욱 심해짐이 관찰되었다 (5.8%). T3 세대에서 single copy T-DNA 계통들은 1.3%의 GUS 비활성율을 보인 반면, multi-copy T-DNA 계통에서의 비활성율은 12.6%로 급격히 증가하였다. 유사한 현상이 형질전환 식물체와 정상개체를 교배하여 생산된 F2 계통에서도 관찰되었다 (비활성율 9.9%). 본 실험으로 식물체에 도입된 외래 유전자가 후대에서의 전이과정동안 점진적으로 비활성화되고, 이 현상은 multi copy T-DNA 계통에서 훨씬 심각함이 밝혀졌다.

• 연구논문집
• /
• 통권32호
• /
• pp.5-13
• /
• 2002

This paper deals with the result of an analysis on the effect of the power generation by municipal solid waste(MSW)-refuse derived fuel(RDF) and the introduction of tipping fee sharing between RDF manufacturing and power generation project. As a model case for the MSW generated at small and medium cities in our country, an analysis of the RDF power generation and Tipping fee sharing shows that 1) MSW amount of 7,800* $10^3 $t/y could produce 1,950*$10^3$t/y of RDF 2) Electric power generated by MSW-RDF could supply 290,000 households/year and 98,548 C-t/y of $CO_2$ could be reduced. 3) Tipping Fee should be shared by the considering of the contents of project of both side between RDF Production and power generation.

• Journal of Plant Biotechnology
• /
• 제36권3호
• /
• pp.289-293
• /
• 2009

This study was conducted to determine the inheritance and expression of transgene in descendants ($T_1\;to\;T_2$ generation) of SOD2-transgenic petunia by PCR and RT-PCR analysis. The trangene was segregated as Mendelian inheritance pattern (3:1 or 1:0) in most of $T_1\;and\;T_2$ generation lines. Transgenic homozygous lines were obtained in T2 generation. It was identified that the transgene expressed stably in examined all plants of 6 $T_2$ lines. The representative morphological traits (plant height, flower diameter, and flower color) of $T_2$ plants were compared with those of non-transgenic plants.

• IMMUNE NETWORK
• /
• 제7권1호
• /
• pp.1-9
• /
• 2007

[ $CD4^+CD25^+$ ] regulatory T cells (Tregs) expressing the lineage-specific marker Foxp3 represent an important regulatory T cell that is essential for maintaining peripheral tolerance. Although it was believed that Treg development is solely dependent on the thymus, accumulating evidence demonstrates that Tregs can also be induced in the periphery. Considering the various origins of peripherally developed $CD4^+CD25^+Foxp3^+$ regulatory T cells, it seems likely that multiple factors are involved in the peripheral generation of Tregs.

• 한국환경과학회지
• /
• 제11권12호
• /
• pp.1275-1279
• /
• 2002

This study was performed to improve water demand estimation and analize correlation between generation of domestic sewage and domestic water use. To improve the prediction of water demand estimation, new water demand equation was developed. The results is as follows. $InQ_t = {\beta}_0+{\beta}_1InP_t+{\beta}_2InY_t+{\beta}_3InH_t+{varepsilon}_t$By using the statistical analysis of the "generation of domestic sewage" and "domestic water use", the regression equation between them is formed. The result is as follows. Generation of domestic sewage : 0.8487 $\times$ Domestic water use + 684.57 ($R^2$= 0.972)>$R^2$= 0.972)

• Biomolecules & Therapeutics
• /
• 제24권3호
• /
• pp.244-251
• /
• 2016

Understanding the developmental mechanisms of humoral immunity against intranasal antigens is essential for the development of therapeutic approaches against air-borne pathogens as well as allergen-induced pulmonary inflammation. Follicular helper T (Tfh) cells expressing CXCR5 are required for humoral immunity by providing IL-21 and ICOS costimulation to activated B cells. However, the regulation of Tfh cell responses against intranasal antigens remains unclear. Here, we found that the generation of Tfh cells and germinal center B cells in the bronchial lymph node against intranasal proteinase antigens was independent of $TGF-{\beta}$. In contrast, administration of STAT3 inhibitor STA-21 suppressed the generation of Tfh cells and germinal center B cells. Compared with wild-type OT-II T cells, STAT3-deficient OT-II T cells transferred into recipients lacking T cells not only showed significantly reduced frequency Tfh cells, but also induced diminished IgG as well as IgE specific for the intranasal antigens. Cotransfer study of wild-type OT-II and STAT3-deficient OT-II T cells revealed that the latter failed to differentiate into Tfh cells. These findings demonstrate that T cell-intrinsic STAT3 is required for the generation of Tfh cells to intranasal antigens and that targeting STAT3 might be an effective approach to ameliorate antibody-mediated pathology in the lung.

• 유기물자원화
• /
• 제14권3호
• /
• pp.91-100
• /
• 2006

본 연구에서는 돼지 분 뇨 및 폐수의 오염물 부하를 조사 및 분석함으로써 다양한 형태의 돼지 돈사로부터 발생 및 배출되는 오염물 부하 원단위를 산정한 결과는 다음과 같다. 오염물질의 발생부하 원단위는 돼지의 성장단계에 비례하여 증가하였으나, 돈사의 형태에 따른 발생부하 원단위는 각 돈사별 평균값을 고려할 때 큰 차이를 보이지 않았다. 3계절과 생체 중량을 고려한 평균 분뇨 발생량은 분이 1.49kg/head/d, 뇨가 3.08kg/head/d로서 총 4.57kg/head/d가 발생하였다. 돈사의 형태와 계절을 고려한 최종적인 평균 오염물질 발생부하 원단위는 BOD 199.5g/head/d, $COD_{cr}\;413.5g/head/d$, T-N 27.8g/head/d, T-P가 5.3g/head/d로 나타났다. 시멘트 돈사에서 배출되는 폐수의 배출부하 원단위는 BOD 31.3g/head/d, $COD_{cr}\;95.6g/head/d$, T-N 8.9g/head/d, T-P가 3.1g/head/d로 조사되었다. 분의 수거율(80%, 90%)을 고려한 분의 발생부하 원단위와 시멘트 돈사의 폐수발생 원단위의 합을 슬러리 돈사의 배출부하 원단위와 비교 시, 오염물질과 항목별로 다소의 차이는 있으나 거의 유사하였다.

• 한국물환경학회지
• /
• 제31권3호
• /
• pp.235-240
• /
• 2015

To evaluate the unit load generation and discharge, pig cage test was conducted. Feed intake, drink amount, and urine generation increased growth stage (heavy weight) of the pig more great. However, the sum of the urine and manure did not show a significant difference in the growth stages of pigs. Because of the limit of the experiment, e.g., research period, high pigpen temperature, breed-related stress and etc., it could not be derived the results of the four seasons. Therefore, in order to generalize the results, the feed intakes were calibrated using a NRC (National Research and nutritional requirements of pigs from the Commission) standards. The finalized unit load generation and generation amounts of manure and urine were estimated at BOD 104.1 g/head/d, T-N 21.2 g/head/d, T-P 4.9 g/head/d, manure 0.96 L/d, urine 1.66 L/d with consideration of revised feed intake. Compare to the former research results of MOE (Ministry of Environment, 1999) and NIAS (National Institute of Animal Science, 2008), the generation amounts of manure and urine were similar to the NIAS's values. In case of unit load generation, BOD and T-N were almost similar in all of them. However, the T-P unit load generation of MOE was more difference, e.g., 2.5 times high, compare to this study.

• 한국수정란이식학회지
• /
• 제27권1호
• /
• pp.9-14
• /
• 2012

This study was conducted to analyze the transgenic efficiency and sex ratio in ${\alpha}$-1,3-galactosyltransferase (GalT) knock-out (KO) transgenic pigs according to generation. GalT KO piglets were produced by artificial insemination or natural mating. The transgenic confirmation of GalT KO was evaluated by PCR amplification using specific primers. After electrophoresis, three types of bands were detected such as 2.3 kb single band (Wild), 2.3 and 3.6kb double bands (GalT KO -/+; heterozygote), and 3.6kb single band (GalT KO -/-; homozygote). Transgenic efficiency in F1 generation was 64.5% (23/35) of GalT KO (-/+). In F2 generation, GalT KO transgenic efficiency was 36.4% (21/57, Wild), 47.5% (28/57, GalT KO -/+), and 16.1% (8/57, GalT KO -/-), respectively. Interestingly, no homozygote piglets were born in 6 deliveries among total 11 deliveries, although they were pregnant between male (M) and female (F) $F_1$ heterozygote. In the 5 litters including at least one GalT KO -/- piglet, the transgenic efficiency was 13.3% (2/24, Wild), 51.3% (14/24, GalT KO -/+), and 35.3% (8/24, GalT KO -/-), respectively. The sex ratio of M and F was 40:60 in $F_1$ and 49:51 in $F_2$ generation, respectively. Based on these results, GalT KO transgenic pigs have had a reproductive ability with a normal range of transgenic efficiency and sex ratio.

• Journal of the Korean Society for Industrial and Applied Mathematics
• /
• 제24권3호
• /
• pp.293-303
• /
• 2020

The present work aims to analyzed the transient thermoelastic stress analysis of a thin circular plate with uniform internal heat generation. Initially, the plate is characterized by a parabolic temperature distribution along the z-direction given by T = T₀(r, z) and perfectly insulated at the ends z = 0 and z = h. For times t > 0, the surface r = a is subjected to convection heat transfer with convection coefficient h_c and fluid temperature T_∞. The integral transform method used to obtain the analytical solution for temperature, displacement, and thermal stresses. The associated thermoelastic field is analyzed by making use of the temperature and thermoelastic displacement potential function. Numerical results are carried out with the help of computational software PTC Mathcad Prime-3.1 and shown in figures.