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Kinetics and Mechanism of Mutant O-acetylserine Sulfhydrylase-A (C43S) from Salmonella typhimurium LT-2

  • Yoon, Moon-Young
    • BMB Reports
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    • v.29 no.3
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    • pp.210-214
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    • 1996
  • The pH dependence of the kinetic parameters of mutant O-acetylserine sulfhydrylase (OASS) from Salmonella typhimurium LT-2 has been determined in order to obtain information on the chemical mechanism. The initial velocity pattern obtained by varying the concentrations of OAS at several fixed concentrations of TNB, shows an intersection on the left of the ordinate at pH 7.0, indicating that the kinetic mechanism is a sequential mechanism in which substrate inhibition by OAS is observed while the wild type enzyme showed a ping pong mechanism. The values of $V/E_t$, $V/K_{OAS}E_{t}$ and $V/K_{TNB}E_{t}$ decreased by about 68%, 14% and 16% as compared with the wild type enzyme. The $V/K_{OAS}E_{t}$ is a pK of 6.5 on the acid side of the pH profile, and the $V/K_{TNB}$ is pH independent. As compared with the wild type enzyme, the pKs in the V/K profiles are shifted, reflecting that binding of the cofactor in free E:OAS is less asymmetric.

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A Study on the Dielectric and Piezoelectric properties of the Pb(SbS11/2TSnS11/2T)OS13T-PbTiOS13T-PbZrOS13T Ceramics (Pb(Sb1/2Sn1/2)O3-PbTiO3-PbZrO3 세라믹스의 유전 및 압전 특성에 관한 연구)

  • 정장호;류기원;이성갑;이영희
    • The Transactions of the Korean Institute of Electrical Engineers
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    • v.41 no.5
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    • pp.517-524
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    • 1992
  • In this study, 0.10Pb(SbS11/2TSnS11/2T)OS13T-(0.90-x)PbZrOS13T (0.25 x 0.40) ceramics were fabricated by the atmospheric method. The sintering temperature and time were 1250[$^{\circ}C$] and 2[2hr], respectively. The structureal, dielectric and piezoelectric properties with composition of PbTiOS13T were studied. As the results of XRD ans SEM, the crystal structure of a specimen was rhombohedral, lattice constant and average grain size were decreased with increasing the contents of PbTiOS13T. Relative dielectric constant and Curie temperature were increased with increasing the contents of PbTiOS13T, 0.10PSS-0.40PT-0.50PZ specimen had the highest values of 904 and 265[$^{\circ}C$], respectively. In increasing of PbTiOS13T contents form 25[mol%] to 40[mol%], piezoelectric charge constant and electromechanical coupling factors were increased form 114[pC/N] to 142[pC/N], 17[%] to 24[%] and mechanical quality factor were decreased with increasing the contents of PbTiOS13T. In the 0.10PSS-0.40PT-0.50PZ specimens, those values were 14.2[kV/cm] and 9.43[x10S0-6TC/cmS02T], resectively.

Comparison of Phosphorus and Nitrogen Removal Characteristics between A2/O and Modified Phostrip Processes (A2/O공정과 수정 Phostrip공정과의 질소 및 인제거 특성비교)

  • Kim, Kwang-Soo;Kim, I-Tai
    • Journal of Korean Society on Water Environment
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    • v.21 no.6
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    • pp.664-668
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    • 2005
  • An experimental study for the comparison of nitrogen and phosphorus removal efficiencies between $A^2/O$ and modified Phostrip (M-Phostrip process) were carried out with bench-scale reactors. In case of nitrogen removal efficiencies both of processes showed similar ones when influent organic loadings were high. However, M-phostrip process was more effective than $A^2/O$ at low organic loadings. This is why M-phostrip process consumes the whole mass of influent organics as a carbon sources for denitrification in anoxic reactor but the anoxic reactor of $A^2/O$ process utilizes the residual carbon followed by consumming a part of influent carbon for phosphorus release in anaerobic reactor. $A^2/O$ process required the influent COD/T-P and COD/TKN ratios were more than 56 and 10, respectively, to take place the phosphorus release in anaerobic process and phosphorus uptake in oxic process. However, the luxury uptake of phosphorus in M-phostrip process was not affected by influent COD/T-P and COD/TKN ratios and the adverse effect of nitrate in return sludge introduced to the p-stripper from the 2nd clarifier was not significant due to the configurational advantage of the p-stripper.

Solubility, vapor pressure, duhring and enthalpy-concentration charts of$H_2$O/(LiBr+$CaC1_2$) solution as a new working fluid ($H_2$O/(LiBr+$CaC1_2$) 3성분계 작동매체의 용해도, 증기압측정 및 듀링 선도, 엔탈피-농도 선도 작성)

  • 이형래;구기갑;정시영
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.10 no.6
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    • pp.666-673
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    • 1998
  • Solubilities (LiBr+$CaC1_2$) in water were measured at temperatures form 267.51 to 306.17K for $CaC1_2$ (LiBr+$CaC1_2$)=0.24 by mole. Experimental data were correlated with polynomial equations. Average absolute deviations between the measured and calculated values were 0.31% at concentration smaller than 60wt% and 0.41% at concentration larger than 60wt%, respectively. Vapor pressures were measured at temperatures from 296.75 to 436.75K and concentrations from 40 to 70wt%. Vapor pressure data were fitted to a Antoine-type equation and average absolute deviation was 2.98%. The P-T-X chart and H-T-X chart of $H_2O$/(LiBr+$CaC1_2$) system were constructed by using the correlation equations of solubility, vaper pressure, and heat capacity. The P-T-X chart indicates that $H_2O$/(LiBr+$CaC1_2$) system has potential as a possible working fluid for air-cooled absorption chillers.

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Prodiction of Walleye Pollock , Theragra Chalcogramma , Landings in Korea by Time Series Analysis : AIC (시계열분석을 이용한 한국 명태어업의 어획량 예측 : AIC)

  • Park, Hae-Hoon;Yoon, Gab-Dong
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.32 no.3
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    • pp.235-240
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    • 1996
  • Forecasts of monthly landings of walleye pollock, Theragra chalcogramma, in Korea were carried out by the seasonal Autoregressive Integrated Moving Average(ARlMA) model. The Box - Cox transformation on the walleye pollock catch data handles nonstationary variance. The equation of Box - Cox transformation was Y'=($Y^0.31$_ 1)/0.31. The model identification was determined by minimum AIC(Akaike Information Criteria). And the seasonal ARlMA model is presented (1- O.583B)(1- $B^1$)(l- $B^12$)$Z_t$ =(l- O.912B)(1- O.732$B^12$)et where: $Z_t$=value at month t ; $B^p$ is a backward shift operator, that is, $B^p$$Z_t$=$Z_t$-P; and et= error term at month t, which is to forecast 24 months ahead the walleye pollock landings in Korea. Monthly forecasts of the walleye pollock landings for 1993~ 1994, which were compared with the actual landings, had an absolute percentage error(APE) range of 20.2-226.1 %. Thtal observed annual landings in 1993 and 1994 were 16, 61OM/T and 1O, 748M/T respectively, while the model predicted 10, 7 48M/T and 8, 203M/T(APE 37.0% and 23.7%, respectively).

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Electrical Properties of ZnO Varistors with Variation of Co3O4 (Co3O4 첨가량 변화에 따른 ZnO Varistor의 전기적 특성)

  • Cho, Hyun-Moo;Lee, Sung-Gap
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.17 no.11
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    • pp.1186-1191
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    • 2004
  • ZnO varistor ceramics which were fabricated with variation of added of 0.5~1.0 mol% Co$_3$O$_4$, were sintered at 1150 $^{\circ}C$. In the specimen added 0.7 mol% Co$_3$O$_4$, sintered density was 6.03 g/㎤ and electrical properties were superior to any other compositions. The nonlinear coefficient a and clamping voltage ratio were 83 and 1.35, respectively. But, endurance surge current in the specimen added 0.5 mol% Co$_3$O$_4$ was 7000 A/$\textrm{cm}^2$, and deviation of varistor voltage was Δ-3.23 %. As P.C.T and T.C.T environmental test were succeed in all specimens, and deviation of varistor voltage in the specimen added 0.6 mol% Co$_3$O$_4$ was Δ-0.81 %. All specimens showed good leakage current property on the High Temperature Continuous Load Test(HTCLT) for 1000 hr at 85 $^{\circ}C$ and variation rate of the varistor voltage below Δ-2.0 %.

A Marriage Problem Algorithm Based on Duplicated Sum of Inter-Preference Moving Method (중복된 최소 상호-호감도 합 이동방법을 적용한 결혼문제 알고리즘)

  • Lee, Sang-Un
    • Journal of the Korea Society of Computer and Information
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    • v.20 no.5
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    • pp.107-112
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    • 2015
  • This paper proposes a simplified algorithm devised to obtain optimal solution to the marriage problem. In solving this problem, the most widely resorted to is the Gale-Shapley algorithm with the time complexity of $O({\mid}V{\mid}^2{\mid}E{\mid})$. The proposed algorithm on the other hand firstly constructs a $p_{ij}$ matrix of inter-preference sum both sexes' preference over the opposite sex. Secondly, it selects $_{min}p_i$ from each row to establish ${\mid}p_{.j}{\mid}{\geq}2,j{\in}S$, ${\mid}p_{.j}{\mid}=1$, $j{\in}H$, ${\mid}p_{.j}{\mid}=0$, $j{\in}T$. Finally, it shifts $_{min}\{_{min}p_{ST},p_{SH}+p_{HT\}$ for $_{min}P_{ST}$ of $S{\rightarrow}T$ and $p_{SH}+p_{HT}$, $p_{HT}<_{min}p_{ST}$ of $S{\rightarrow}H$, $H{\rightarrow}T$. The proposed algorithm has not only improved the Gale-Shapley's algorithm's complexity of $O({\mid}V{\mid}^2{\mid}E{\mid})$ to $O({\mid}V{\mid}^2)$ but also proved its extendable use on unbalanced marriage problems.

Finding Approximate Covers of Strings (문자열의 근사커버 찾기)

  • Sim, Jeong-Seop;Park, Kun-Soo;Kim, Sung-Ryul;Lee, Jee-Soo
    • Journal of KIISE:Computer Systems and Theory
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    • v.29 no.1
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    • pp.16-21
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    • 2002
  • Repetitive strings have been studied in such diverse fields as molecular biology data compression etc. Some important regularities that have been studied are perods, covers seeds and squares. A natural extension of the repetition problems is to allow errors. Among the four notions above aproximate squares and approximate periodes have been studied. In this paper, we introduce the notion of approximate covers which is an approximate version of covers. Given two strings P(|P|=m) and T(|T|=n) we propose and algorithm with finds the minimum distance t such that P is a t-approximate cover of T. The algorithm take O(m,n) time for the edit distance and $O(mn^2)$ time of finding a string which is an approximate cover of T is minimum distance is NP-complete.

Growth, Yield and Nutrient Uptake of Radish as Affected by Amount of Organic Fertilizer in a Volcanic Ash soil (화산회토에서 유기질비료 시용량이 무 생장과 수량 및 양분흡수에 미치는 영향)

  • Kim, Yu-Kyoung;Cho, Young-Yuen;Oh, Han-Jun;Kang, Ho-Jun;Yang, Sang-Ho;Moon, Bong-Chun;Jwa, Chang-Sook
    • Korean Journal of Organic Agriculture
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    • v.23 no.4
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    • pp.829-846
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    • 2015
  • This study was conducted to determine the effects of organic fertilization rates on the nutrient accumulation and recovery in radish (Raphanus sativus L.) as well as growth and yield of radish in Jeju island. An understanding the relationships between organic fertilization rate, crop nutrient recovery and crop yield can assist in making organic fertilizer recommendation which balances crop value and environmental risk in organic cultivation. Nitrogen (T-N), phosphate ($P_2O_5$) and potassium ($K_2O$) were applied at 0, 115-35-40, 230-70-80 (standard application rate), 460-140-160, 230-200-100 (recommended application rate) and 158-53-35 kg/ha (customary application rate), respectively as the broadcast application of mixed organic fertilizer (N 4.5% - $P_2O_5$ 1.5% - $K_2O$ 1%) in combination with langbeinite ($K_2O$ 22%), 100% at sowing period. The organic fertilizer was made of organic materials like oil cakes. Total yield of radish, as fresh weight of roots, increased with increasing organic fertilizer doses to a maximum at rate of standard or soil-testing application. Nitrogen, phosphate and potassium accumulations of radish increased curvilinearly with increasing organic fertilization rate to a maximum at rate of N 460 - $P_2O_5$ 140 - $K_2O$ 160 kg/ha. However, nitrogen, phosphate and potassium use efficiency of applied organic fertilizer decreased curvilinearly or linearly with increasing organic fertilization rate. Application of organic fertilizer in combination with langbeinite (as a potassium source) had significant effect on the yield of radish. Organic fertilization on a basis of standard or soil-testing application rate is recommended for maximun radish yield in organic cultivation.

Effect of Opuntia ficus-indica var. saboten Powder Addition on Quality Characteristics of Sausage (백년초 분말 첨가가 소시지의 품질 특성에 미치는 영향)

  • Jin, Sang-Keun;Shin, Daekeun;Hur, In-Chul
    • Journal of agriculture & life science
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    • v.45 no.6
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    • pp.125-134
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    • 2011
  • This study was carried out to investigate the effects of Opuntia ficus-indica var. saboten (OFIS) powder addition replacing nitrite on quality characteristics of sausages stored at $4^{\circ}C$ during 15 days. All samples were divided into six different treatments as follow: C (non-sodium nitrite and -O. ficus-indica var. saboten powder), T1 (0.014% sodium nitrite), T2 (0.014% sodium nitrite + 0.5% O. ficus-indica var. saboten powder), T3 (0.014% sodium nitrite + 1.0% Opuntia ficus-indica var. saboten powder), T4 (0.5% O. ficus-indica var. saboten powder) and T5 (1.0% O. ficus-indica var. saboten powder), respectively. The lightness (CIE $L^*$) of negative control (C) was higher than other treatments (p<0.05), and the redness (CIE $a^*$) of T5 treatment showed an intermediate value between T1 and T3 treatment. Yellowness (CIE $b^*$), water holding capacity (WHC) and cohesiveness, springiness and chewiness of T5 treatment were higher than other treatments (p<0.05). However moisture content, pH and whiteness of T1 treatment were the highest (p<0.05). No differences in sensory evaluation were observed between T1 and T5 treatment (p>0.05). Therefore, the results indicate that OFIS powder improves color and texture properties of sausage, however, specific study related to self-life due to the addition of OFIS powder to sausages may be conducted.