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Intratypic Variants of HPV-16 E6jE7 Oncogene Isolated from Sexually High-Risk Women in Busan. (부산지역 유흥업소 종사여성으로부터 분리된 HPV16형의 발암유전자(E6/E7) 돌연변이 유형 분석)

  • Min, Sang-Kee;Kim, Sung-Soon;Choi, Byeong-Sun;Jang, Dai-Ho;Lee, Mee-Ok;Choi, Seung-Hwa;Kim, Nam-Ho;Park, Yon-Koung;Jeong, Yeong-A;Kim, Seong-Joon;Bin, Jae-Hun;Park, Ho-Kuk
    • Journal of Life Science
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    • v.19 no.6
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    • pp.765-769
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    • 2009
  • Recent studies have reported that the distribution of HPV-16 sequence variation differs geographically, and more specifically that HPV-16 E6/E7 intratypic variants might carry a high risk for development of ICC (invasive cervical cancer) and CIN (cervical intraepithelial neoplasia) in a given population. To investigate the genetic diversities of HPV-16 E6/E7 oncogene by region, we collected nineteen HPV-16 isolates from sexually high-risk women in Busan, and analyzed the HPV-16 E6/E7 coding regions (nt 34 to 880) with HPV-16 E6/E7 specific PCR amplification. At the nucleotide levet eleven variants of the E6 genes and nine variants of the E7 genes were identified as follows: E6 T178G (n=l1), E6 T178A (n=l), E6 T350G (n=3), E6 A442C (n=2), E6 AI04T, E6 All1G, E6 C116T, E6 G145T, E6 T183G, E6 C335T, E6 G522C and E7 A647G (n=12), E7 A645C, E7 A777C, E7 G663A, E7 T732C, E7 T760C, E7 A775T, E7 T789C and E7 T795G, respectively. At the amino acid levet the isolated HPV-16 E6 and E7 genes showed eleven E6 variants: E6 D25E (n=12), E6 L83V (n=4), E6 E113D (n=2), E6 MIL, E6 Q3R, E6 P5S, E6 Q14H, E6 D25N, E6 127R, E6 H78Y, E6 C140S and three E7 variants: N29S (n=12), L28F, T72S. HPV16 E6 L83V, the dominant variant in the Caucasian population, showed relatively low frequencies in our study population. We elucidated that the dominant HPV-16 E6/E7 variants were HPV-16 E6 D25E (63.2%) and HPV-16 E7 N29S (63.2%), which were phylogenetically included in Asian lineage. Further study is needed to evaluate the risk of cervical cancer related HPV-16 E6/E7 intratypic variants in the Korean population.

Growth curve estimates for wither height, hip height, and body length of Hanwoo steers (Bos taurus coreanae)

  • Park, Hu-Rak;Eum, Seung-Hoon;Roh, Seung-Hee;Sun, Du-Won;Seo, Jakyeom;Cho, Seong-Keun;Lee, Jung-Gyu;Kim, Byeong-Woo
    • Korean Journal of Agricultural Science
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    • v.44 no.3
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    • pp.384-391
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    • 2017
  • Growth curves in Hanwoo steers were estimated by Gompertz, Von Bertalanffy, Logistic, and Brody nonlinear models using growth data collected by the Hanwoo Improvement Center from a total of 6,973 Hanwoo (Bos taurus coreanae) steers 6 to 24 months old that were born between 1996 and 2015. The data included three parameters: A, mature size of body measurement; b, growth ratio; and, k, intrinsic growth rate. Nonlinear regression equations for wither height according to Gompertz, Von Bertalanffy, Logistic, and Brody models were $Y_t=144.7e^{-0.5869e^{-0.00301t}}$, $Y_t=145.3(1-0.1816e^{-0.00284t})^3$, $Y_t=143.1(1+0.7356e^{-0.00352t})^{-1}$, and $Y_t=146.8(1+0.4700e^{-0.00249t})^1$, respectively, while those for hip height were $Y_t=144.5e^{-0.5549e^{-0.00312t}}$, $Y_t=145.0(1-0.1724e^{-0.00295t})^3$, $Y_t=143.1(1+0.6863e^{-0.00360t})^{-1}$, and $Y_t=146.2(1+0.4501e^{-0.00263t})^1$, respectively. Equations for body length $Y_t=174.1e^{-0.8342e^{-0.00289t}}$, $Y_t=175.8(1-0.2500e^{-0.00265t})^3$, $Y_t=170.0(1+1.1548e^{-0.00363t})^{-1}$, and $Y_t=180.3(1+0.6077e^{-0.00215t})^1$, respectively, for the same models. Among the four models, the Brody model resulted in the lowest mean square error, with mean square errors of 31.79, 30.57, and 42.13, respectively, for wither height, hip height, and body length. Also, an estimated birth wither height, birth hip height, and birth body length (77.98, 80.57, and 70.97 cm, respectively) were lower in the Brody model than in other models. An inflection point was not observed during the growth phase of Hanwoo steer according to the growth curves calculated using Gompertz, Von Bertalanffy, and Logistic models. Based on the results, we concluded that the regression equation using the Brody model was the most appropriate among the four growth models. To obtain more accurate parameters, however, using data from a wider production period (from birth to shipping) would be required, and the development of a suitable model for body conformation traits would be needed.

CONDITIONAL FORUIER-FEYNMAN TRANSFORM AND CONVOLUTION PRODUCT FOR A VECTOR VALUED CONDITIONING FUNCTION

  • Kim, Bong Jin
    • Korean Journal of Mathematics
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    • v.30 no.2
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    • pp.239-247
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    • 2022
  • Let C0[0, T] denote the Wiener space, the space of continuous functions x(t) on [0, T] such that x(0) = 0. Define a random vector $Z_{\vec{e},k}:C_0[0,\;T] {\rightarrow}{\mathbb{R}}^k$ by $$Z_{\vec{e},k}(x)=({\normalsize\displaystyle\smashmargin{2}{\int\nolimits_0}^T}\;e_1(t)dx(t),\;{\ldots},\;{\normalsize\displaystyle\smashmargin{2}{\int\nolimits_0}^T}\;ek(t)dx(t))$$ where ej ∈ L2[0, T] with ej ≠ 0 a.e., j = 1, …, k. In this paper we study the conditional Fourier-Feynman transform and a conditional convolution product for a cylinder type functionals defined on C0[0, T] with a general vector valued conditioning functions $Z_{\vec{e},k}$ above which need not depend upon the values of x at only finitely many points in (0, T] rather than a conditioning function X(x) = (x(t1), …, x(tn)) where 0 < t1 < … < tn = T. In particular we show that the conditional Fourier-Feynman transform of the conditional convolution product is the product of conditional Fourier-Feynman transforms.

A Research for the Development of the Transmission Error Measuring System and Transmission Error Specification for the Gear Noise Quality (기어 소음 품질 안정화를 위한 전달오차 측정 시스템 개발 및 전달오차 스펙 개발에 대한 연구)

  • Lee, Sang Hwa;Lee, Hyun Ku;Kang, Seok Chan;Kim, Si Yoong;Yoo, Dong Kyu;Won, Kwang Min;Chae, Geum Muk;Lee, Tae Hwi
    • Transactions of the Korean Society for Noise and Vibration Engineering
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    • v.24 no.6
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    • pp.470-475
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    • 2014
  • In the past, transmission error(T.E.) has been identified as one of the main sources of gear whine noise, which cause serious passengers irritation at high frequency. In this study, to identify the correlation of the T.E. and gear whine noise, a T.E. measuring system was developed. By comparing the T.E. and gear whine noise level, T.E. target value which can represent the gear noise was proposed. The conclusions are followed: (I) It is possible to measure T.E. of the gear pairs (II) Similar trends are observed between T.E. and gear whine noise. (III) By observing the T.E. pattern, presence of sideband noise can be predicted. (IV) Proposed T.E. target value can be adopted to the gear manufacturing for gear noise quality management.

OSCILLATION AND NONOSCILLATION THEOREMS FOR NONLINEAR DIFFERENTIAL EQUATIONS OF SECOND ORDER

  • Kim, Rak-Joong;Kim, Dong-Il
    • Journal of the Korean Mathematical Society
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    • v.44 no.6
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    • pp.1453-1467
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    • 2007
  • By means of a Riccati transform some oscillation or nonoscillation criteria are established for nonlinear differential equations of second order $$(E_1)\;[p(t)|x#(t)|^{\alpha}sgn\;x#(t)]#+q(t)|x(\tau(t)|^{\alpha}sgn\;x(\tau(t))=0$$. $$(E_2),\;(E_3)\;and\;(E_4)\;where\;0<{\alpha}$$ and $${\tau}(t){\leq}t,\;{\tau}#(t)>0,\;{\tau}(t){\rightarrow}{\infty}\;as\;t{\rightarrow}{\infty}$$. In this paper we improve some previous results.

Studies on the Fishery Biology of Pomfrets, Pampus spp. in the Korean Waters 3. Age and Growth of Korean Pomfret, Pampsu echinogaster, from the East China Sea (한국근해 병어류의 자원생물학적 연구 3. 동지나해산 덕대의 연령과 성장)

  • KANG Yong Joo;LEE Dong Woo;HONG Byung Que;KIM Young Sub
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.22 no.5
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    • pp.281-290
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    • 1989
  • Growth of the Korean pomfret, Pampus echinogaster, captured in the East China Sea from January to December 1987, was estimated by annual rings on the vertebral centrum. The time of annual ring formation was approximately during September and October. Von Ber-talanffy growth equations derived from the mean back-calculated fork length(cm) at each age were as follows: for females, $L_{t}=29.17(1-e xp(-0.3213(t+0.7601)))$, for males, $L_t=28.21(1-e xp(-0.3169(t+0.8284)))$, and for sexes combined, $L_t=29.00(1-e xp(-0.3228(t+0.7361)))$, Seasonal growth equations derived from the mean observed fork length (cm) at age by month were as follows: for females, $L_t=29.2(1-e xp(-0.3458(t+0.8182)+(0.3458/2\pi)sin2\pi(t+0.027)))$, and for males, $L_t=28.2(1-e xp(-0.2789(t+1.5913)+(0.2789/2\pi)sin2\pi(t-0.1062)))$.

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AN ACTION OF A GALOIS GROUP ON A TENSOR PRODUCT

  • Hwang, Yoon-Sung
    • Communications of the Korean Mathematical Society
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    • v.20 no.4
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    • pp.645-648
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    • 2005
  • Let K be a Galois extension of a field F with G = Gal(K/F). Let L be an extension of F such that $K\;{\otimes}_F\;L\;=\; N_1\;{\oplus}N_2\;{\oplus}{\cdots}{\oplus}N_k$ with corresponding primitive idempotents $e_1,\;e_2,{\cdots},e_k$, where Ni's are fields. Then G acts on $\{e_1,\;e_2,{\cdots},e_k\}$ transitively and $Gal(N_1/K)\;{\cong}\;\{\sigma\;{\in}\;G\;/\;{\sigma}(e_1)\;=\;e_1\}$. And, let R be a commutative F-algebra, and let P be a prime ideal of R. Let T = $K\;{\otimes}_F\;R$, and suppose there are only finitely many prime ideals $Q_1,\;Q_2,{\cdots},Q_k$ of T with $Q_i\;{\cap}\;R\;=\;P$. Then G acts transitively on $\{Q_1,\;Q_2,{\cdots},Q_k\},\;and\;Gal(qf(T/Q_1)/qf(R/P))\;{\cong}\;\{\sigma{\in}\;G/\;{\sigma}-(Q_1)\;=\;Q_1\}$ where qf($T/Q_1$) is the quotient field of $T/Q_1$.

Signal transduction of C-terminal phosphorylation sites for equine follicle stimulating hormone receptor (eFSHR)

  • Seong, Hoon-Ki;Choi, Seung-Hee;Byambaragchaa, Munkhzaya;Min, Kwan-Sik
    • Journal of Animal Reproduction and Biotechnology
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    • v.35 no.2
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    • pp.155-162
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    • 2020
  • Equine follicle stimulating hormone receptor (eFSHR) has a large extracellular domain and an intracellular domain containing approximately 10 phosphorylation sites within the G protein-coupled receptor. This study was conducted to analyze the function of phosphorylation sties at the eFSHR C-terminal region. We constructed a mutant of eFSHR, in which the C-terminal cytoplasmic tail was truncated at residue 641 (eFSHR-t641). This removed 10 potential phosphorylation sites from the C-terminal region of the intracellular loop. The eFSHR-wild type (eFSHR-wt) and eFSHR-t641 cDNAs were subcloned into the pCMV-ARMS1-PK2 expression vector. These plasmids were transfected into PathHunter CHO-K1 Parental cells expressing β-arrestin 2 enzyme acceptor fusion protein and analyzed for agonist-induced cAMP response. The cAMP response in cells expressing eFSHR-t641 was lower than the response in cells expressing eFSHR-wt. EC50 values of eFSHR-wt and eFSHR-t641 were 1079 ng/mL and 1834 ng/mL, respectively. eFSHR-t641 was approximately 0.58-fold compared with that of eFSHR-wt. The maximal response in eFSHR-wt and eFSHR-t641 was 24.7 nM and 16.7 nM, respectively. The Rmax value of phosphorylation sites in eFSHR-t641 was also decreased to approximately 68.4% of that in eFSHR-wt. The collective data implicate that the phosphorylation sites in the eFSHR C-terminal region have a pivotal role in signal transduction in PathHunter CHO-K1 cells, and indicate that β-arrestin is involved in coupling the activated receptors to the internalization system.

A Study on the influence of job transfer in E&T programs characteristics & organizational situational characteristics of the medium and small-sized hospitals (중소병원의 교육훈련 프로그램 특성 및 조직상황적 특성이 직무전이에 미치는 영향에 관한 연구)

  • Kim, Young-Hyuk
    • Korea Journal of Hospital Management
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    • v.16 no.4
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    • pp.131-160
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    • 2011
  • The purpose of this study was 1) to understand the job transfer in education & training(E&T) programs & organizational situational characteristics, and 2) to analyze the effects of the E&T programs & organizational situational characteristic groups on each of the levels of job transfer. There is intimate relationship between E&T programs characteristics & organizational situational characteristics and job transfer, so we can't think of them separately. With this point of view the most frequently discussed problem is how to apply the theory in actual job, but in reality it's true that the improvement of job transfer can't reach the expectations of the E&T programs. In literature view study, the concepts of job transfer in E&T, the general theory on E&T, and the actual condition and points at issue of the existing E&T practices in the medium and small-sized hospitals were defined. As the results from empirical analysis, we could find followings: First, in evaluation of the job transfer, demographic characteristics (seniority, occupational category, position) variables have a significant different. Second, in degree of the job transfer, E&T programs characteristics(contents of E&T, design of E&T, conditions of E&T, teaching ability of lecturer, methods of E&T) variables have a different influence upon each of the evaluation levels of E&T. Third, support of CEO variables have a different influence on the job transfer according to contents, design, conditions of the E&T programs. Fourth, support of co-works variables have a different influence on the job transfer according to design, conditions, method of the E&T programs. Fifty, support of organizational atmosphere variables have a different influence on the job transfer according to contents, design, conditions of the E&T programs.

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