• Journal of Microbiology and Biotechnology
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• v.16 no.10
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• pp.1529-1536
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• 2006

We cloned a gene of ompH(D:4) from pigs infected with P. multocida D:4 in Korea [16]. The gene is composed of 1,026 nucleotides coding 342 amino acids (aa) with a signal peptide of 20 aa (GenBank accession number AY603962). In this study, we analyzed the ability of the ompH(D:4) to induce protective immunity against a wild-type challenge in mice. To determine appropriate epitope(s) of the gene, one full and three different types of truncated genes of the ompH(D:4) were constructed by PCR using pET32a or pRSET B as vectors. They were named ompH(D:4)-F (1,026 bp [1-1026] encoding 342 aa), ompH(D:4)-t1 (693 bp [55-747] encoding 231 aa), ompH(D:4)-t2 (561 bp [187-747] encoding 187 aa), and ompH(D:4)-t3 (540 bp [487-1026] encoding 180 aa), respectively. The genes were successfully expressed in Escherichia coli BL21(DE3). Their gene products, polypeptides, OmpH(D:4)-F, -t1, -t2, and -t3, were purified individually using nickel-nitrilotriacetic acid (Ni-NTA) affinity column chromatography. Their $M_rs$ were determined to be 54.6, 29, 24, and 23.2 kDa, respectively, using SDS-PAGE. Antisera against the four kinds of polypeptides were generated in mice for protective immunity analyses. Some $50{\mu}g$ of the four kinds of polypeptides were individually provided intraperitoneally with mice (n=20) as immunogens. The titer of post-immunized antiserum revealed that it grew remarkably compared with pre-antiserum. The lethal dose of the wild-type pathogen was determined at $10{\mu}l$ of live P. multocida D:4 through direct intraperitoneal (IP) injection, into post-immune mice (n=5, three times). Some thirty days later, the lethal dose ($10{\mu}l$) of live pathogen was challenged into the immunized mouse groups [OmpH(D:4)-F, -t1, -t2, and -t3; n=20 each, two times] as well as positive and negative control groups. As compared within samples, the OmpH(D:4)-F-immunized groups showed lower immune ability than the OmpH(D:4)-t1, -t2, and -t3. The results show that the truncated-OmpH(D:4)-t1, -t2, and -t3 can be used for an effective vaccine candidate against swine atrophic rhinitis caused by pathogenic P. multocida (D:4) isolated in Korea.

• Journal of the Korean Mathematical Society
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• v.45 no.4
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• pp.1075-1087
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• 2008

We study a class of KMS-symmetric quantum Markovian semigroups on a quantum spin system ($\mathcal{A},{\tau},{\omega}$), where $\mathcal{A}$ is a quasi-local algebra, $\tau$ is a strongly continuous one parameter group of *-automorphisms of $\mathcal{A}$ and $\omega$ is a Gibbs state on $\mathcal{A}$. The semigroups can be considered as the extension of semi groups on the nontrivial abelian subalgebra. Let $\mathcal{H}$ be a Hilbert space corresponding to the GNS representation con structed from $\omega$. Using the general construction method of Dirichlet form developed in [8], we construct the symmetric Markovian semigroup $\{T_t\}{_t_\geq_0}$ on $\mathcal{H}$. The semigroup $\{T_t\}{_t_\geq_0}$ acts separately on two subspaces $\mathcal{H}_d$ and $\mathcal{H}_{od}$ of $\mathcal{H}$, where $\mathcal{H}_d$ is the diagonal subspace and $\mathcal{H}_{od}$ is the off-diagonal subspace, $\mathcal{H}=\mathcal{H}_d\;{\bigoplus}\;\mathcal{H}_{od}$. The restriction of the semigroup $\{T_t\}{_t_\geq_0}$ on $\mathcal{H}_d$ is Glauber dynamics, and for any ${\eta}{\in}\mathcal{H}_{od}$, $T_t{\eta}$, decays to zero exponentially fast as t approaches to the infinity.

• Korean Journal of Microbiology
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• v.29 no.6
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• pp.387-391
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• 1991

Methylobacterium extorquens AM1 growing on methanol as a sole source of carbon and energy was found to grow most rapidly (t$t_{d}$ =6h) at 30.deg.C in a mineral medium (pH 7.0) containing 0.5% (v/v) methanol which was agitated at 200 rpm (optimal cultivation condition). Cells grown under the optimal cultivation condition contained more spermidine, but less putrescine, than the cells grown on 2.5%(v/v) ( $t_{d}$ =8h ) or at 20.deg.C ( $t_{d}$ =8h ). Cells cultivated under the optimal condition was found to contain more spermidine than the cells grown at pH 6.0 (( $t_{d}$ =7h ) and pH 8.0 ($t_{d}$ =7.3h). the cells growing at the stationary phase under the optimal condition accumulated more spermine or putrescine than the cells growing at the same phase on 2.5%(v/v) methanol or at pH 6.0 and pH 8.0, respectively. M. extorquens AM1 grown in a medium agitated at 100 rpm ( $t_{d}$ =8.8h ) contained less spermidine and spermine than the cells grown under the optimal cultivation condition.

• Bulletin of the Korean Mathematical Society
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• v.44 no.4
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• pp.731-742
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• 2007

Let X be a Banach space, $A:D(A){\subset}X{\rightarrow}X$ the generator of a compact $C_0-semigroup\;S(t):X{\rightarrow}X,\;t{\geq}0$, D a locally closed subset in X, and $f:(a,b){\times}C([-q,0];X){\rightarrow}X$ a function of Caratheodory type. The main result of this paper is that a necessary and sufficient condition in order that D be a viable domain of the semi linear differential equation of retarded type $$u#(t)=Au(t)+f(t,u_t),\;t{\in}[t_0,\;t_0+T],{u_t}_0={\phi}{\in}C([-q,0];X)$$ is the tangency condition $$\limits_{h{\downarrow}0}^{lim\;inf\;h^{-1}d(S(h)v(0)+hf(t,v);D)=0}$$ for almost every $t{\in}(a,b)$ and every $v{\in}C([-q,0];X)\;with\;v(0){\in}D$.

• Journal of the Korean Mathematical Society
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• v.51 no.4
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• pp.791-815
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• 2014

We show amongst other that if $f,g:[a,b]{\rightarrow}\mathbb{C}$ are two functions of bounded variation and such that the Riemann-Stieltjes integral $\int_a^bf(t)dg(t)$ exists, then for any continuous functions $h:[a,b]{\rightarrow}\mathbb{C}$, the Riemann-Stieltjes integral $\int_{a}^{b}h(t)d(f(t)g(t))$ exists and $${\int}_a^bh(t)d(f(t)g(t))={\int}_a^bh(t)f(t)d(g(t))+{\int}_a^bh(t)g(t)d(f(t))$$. Using this identity we then provide sharp upper bounds for the quantity $$\|\int_a^bh(t)d(f(t)g(t))\|$$ and apply them for trapezoid and Ostrowski type inequalities. Some applications for continuous functions of selfadjoint operators on complex Hilbert spaces are given as well.

• Communications of the Korean Mathematical Society
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• v.9 no.4
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• pp.819-823
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• 1994

If H is a Hilbert space, then an operator $T : D(T) \subset H \to H$ is said to be monotone if $$ (x-y, Tx-Ty) \geq 0$$ for any x, y in D(T). Many authors [1], [4] obtained the existence theorem for the equation $y = x + Tx$ for x, given an element y in H and a monotone operator T. On the other hand some iterative methods were applied to the approximations for the solution of the above equation [6], [8]. For example Bruck [2] obtained the iterative solution of the above equation with an explicit error estimate as follows.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.13 no.2
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• pp.1-4
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• 1977

The author determined the relationship between the hauling veloicty and the hydrodynamic resistance of the sablefish pot shaped conic frustum like, and induced the formulae to determine the diameter of the main line and the net horse power of the pot hauler. The results are summarized as follows: 1. The maximum hydrodynamic resistance (with its weight in water) of the pot T(kg), when the bottom webbing is covered by a cloth to imitate the catches are scattered on the bottom, is eatimated as $$ T=120v^{1.1} (0.3{\leqq}v{\leqq}0.8) $$ where v denotes the hauling velocity of the pot in m/sec. 2. When P. P. 3 strand rope is used as main line, the diameter d(mm)is recommended to satisfy the formula $$ d=72 \frac{D}{H} V^{1.1} where H denotes the depth of the fishing ground and D the intervals of the pots linked to the main in m respectively. 3. The pot hauler must displace the net horse power p(ps) of $$ P= \frac{75}{120} \frac{D}{H} v^{2.1}$$

• Proceedings of the Korean Magnestics Society Conference
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• 2008.12a
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• pp.4.2-4.2
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• 2008

• Communications of the Korean Mathematical Society
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• v.19 no.2
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• pp.307-319
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• 2004

In this paper, we establish some new oscillation criteria for the functional differential equations of the form $\frac{d}{dt}$$\frac{1}{a_{n-1}(t)}$$\frac{d}{dt}(\frac{1}{{a_{n-2}(t)}\frac{d}{dt}(...(\frac{1}{a_1(t)}\frac{d}{dt}x(t))...)))^\alpha$ + $\delta[f_1(t,s[g_1(t)],\frac{d}{dt}x[h_1(t)])$ + $f_2(t,x[g_2(t)],\frac{d}{dt}x[h_2(t)])]=0$ via comparing it with some other functional differential equations whose oscillatory behavior is known.

• Journal of the Korean Magnetics Society
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• v.4 no.1
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• pp.52-55
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• 1994

The proton and deuteron spin-lattice relaxation times, $T_{1}(H)$ and $T_{1}(D)$, have been measured in HD between 30 K and 313 K in the pressure of 0.67 - 1.92 atm. The nuclear magnetic resonance frequencies are respectively 358.012 MHz for a proton and 58.958 MHz for a deuteron. From the measurements of $T_{1}(H)$ and $T_{1}(D)$ the ratio of the correlation times ${\tau}_{1}\;and\;{\tau}_{2}$ that are associated with the molecular angular momentum operators was obtained. The nuclear spin-lattice relaxation time at J = 1 state has been observed to have a temperature dependence being proportional to $T^{0.25}$.