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Culture of tissue-cyst forming strain of Toxoplama gondii and the effect of cyclic AMP and pyrimidine salvage inhibitors (Toxoplasma gondii 약독주의 배양과 그 성장에 미치는 cyclic AMP와 pyrimidine salvage 억제제의 영향)

  • 최원영;박성경
    • Parasites, Hosts and Diseases
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    • v.32 no.1
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    • pp.19-26
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    • 1994
  • An in uipo culturing to examine the cyst stage of ToxopLQsma gondii (ME49 strain) was Investigated using murine peritoneal macrophages, and we also examined the effect of CAMP or DHFR Inhibitors on the growth of bradyzoltes. For experiments ICR mice were Injected 1.p. with 1,500 brain cysts. At 1, 3, 5 and 7 days, peritoneal exudates were Isolated and then adherent peritoneal macrophages were cultured for 1,3,5 and 10 days. Growth pattern of bradyzoltes was measured by (3H)-uracil uptake assay and morphological pattern of pseudocysts formed in macrophages was observed Uth Glemsa stain. Mostly bradyzoites were observed In the macrophages extracted at 3 and S days post Infection. After 3 days in vitro, a number of pseudocysts were formed in the macrophages and the size of pseudocysts was increased during further 5 and 10 days in vitro culture. CAMP stimulated the growth of bradyzoltes when in uiuo 3 and 5 days and then in vitro 5 and 10 days conditions were applied. In case of.DHFR Inhibitors, pynmethamlne produced a linearly decremental effect with a cont.-dependent mode but methotrexate was not effective against Intracellular bradyzoltes or pseudocysts In this system. It was suggested that cyst-forming strain of T gondii (ME49 strain) could be maintained and cultivated in uitro by use of murine peritoneal macrophages. In uivo 3 and 5 days and then in uiko 5 and 10 days conditions appeared to be suitable for culturing of bradyzoltes. CAMP and pyrimethamine had an effect of stimulation and inhibition on the growth of bradyzolte, respectively.

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Selective Mechanism of Cyhalofop-butyl ester between Rice(Oryzae sativa L.) and Echinochloa crus-galli - IV. Effect on Enzyme Activity, Biosynthesis of Fatty Acid and Protein in Rice and Echinochloa crus-galli (제초제(除草劑) Cyhalofop-butyl ester의 벼와 피간(間)의 선택성(選擇性) 기작(機作) - IV. 효소활성(酵素活性), 지방산(脂肪酸) 및 단백질합성(蛋白質合成)에 미치는 영향(影響))

  • Park, J.E.;Lee, I.Y.;Park, T.S.;Ryu, G.H.;Kim, Y.K.;Kim, K.U.
    • Korean Journal of Weed Science
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    • v.17 no.2
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    • pp.192-198
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    • 1997
  • This experiment was carried out to determine selectivity of herbicide cyhalofop-butyl ester on enzyme activity, biosynthesis of fatty acid and protein between rice and Echinochloa crus-galli. Activity of the acetyl-CoA carboxylase(ACCase) was inhibited greater in E. crus-galli than in rice by the treatment of cyhalofop-butyl ester. The ACCase activity in E. crus-galli was observed with $I_{50}$ at 1-2ppm of cyhalofop-butyl ester, while in rice only at above 10ppm. Cyhalofop-butyl ester also inhibited the biosynthesis of fatty acid by 61% of palmitic acid, 54% of linoleic acid and 41% of linolenic acid in E. crux-galli. In contrast, no significant difference of fatty acid content was observed in rice at 5DAT as compared with the untreated control. Protein patterns of rice between the herbicide treatment and the untreated control were not significantly different, but in E. crus-galli, 3 protein spots were disappeared in between 29KD and 45KD.

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Grounding Line of Campbell Glacier in Ross Sea Derived from High-Resolution Digital Elevation Model (고해상도 DEM을 활용한 로스해 Campbell 빙하의 지반접지선 추정)

  • Kim, Seung Hee;Kim, Duk-jin;Kim, Hyun-Cheol
    • Korean Journal of Remote Sensing
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    • v.34 no.3
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    • pp.545-552
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    • 2018
  • Grounding line is used as evidence of the mass balance showing the vulnerability of Antarctic glaciers and ice shelves. In this research, we utilized a high resolution digital elevation model of glacier surface derived by recently launched satellites to estimate the position of grounding line of Campbell Glacier in East Antarctica. TanDEM-X and TerraSAR-X data in single-pass interferometry mode were acquired on June 21, 2013 and September 10, 2016 and CryoSat-2 radar altimeter data were acquired within 15 days from the acquisition date of TanDEM-X. The datasets were combined to generate a high resolution digital elevation model which was used to estimate the grounding line position. During the 3 years of observation, there weren't any significant changes in grounding line position. Since the average density of ice used in estimating grounding line is not accurately known, the variations of the grounding line was analyzed with respect to the density of ice. There was a spatial difference from the grounding line estimated by DDInSAR whereas the estimated grounding line using the characteristics of the surface of the optical satellite images agreed well when the ice column density was about $880kg/m^3$. Although the reliability of the results depends on the vertical accuracy of the bathymetry in this study, the hydrostatic ice thickness has greater influence on the grounding line estimation.

study on the Transfeal Phenomenon of Anti-fashion into Mother-fashion -In the Case of Hippie Look- (저항패션이 모패션으로 전이된 현상에 관한 연구 -히피풍을 중심으로-)

  • 박명희
    • Journal of the Korean Society of Costume
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    • v.28
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    • pp.239-253
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    • 1996
  • The purpose of this dissertation is to exam-ine the hippie look which is one of the most popular fashion trends in the early 90s, in re-lation to the hippie style in 60s and cultural restoration. in order to investigate the re-lationship this dissertation studies the trans-feral phenomenon of anti-fashion into mother fashion in the basis of social and clutural theories. hippie culture is the anti-culture which appeared in America in the late 60s, it tried to create a new culture by subverting the ruling class culture with their combative life style and peculiar appearance which are distinguished from mother culture. First of all to examine the inner symbolic meaning and outer shape of the hippie style I divided the characteristics of the hippies into ideology social value and attitude on sex. in the aspect of ideology the hippie show strong subjectivity while mother culture shows ob-jectivity. in social value the hippie has the resisting chaacteristics while mother culture has the conformative characteristics. In the aspect of sex the hippie tries to break the fixed idea of mother culture. Hippie culture as anti-culture is symbolically expressed in hippie style fashion which is dif-ferent from mother fashion in shape. There-fore the inner meaning and the outer shape of the hippie style shows strong characteristics of anti-fashion. Hippie style as anti-fashion has an effect on high fashion in the late 60s and the early 70s and on hippie look which is restored in 90s. however hippie-look in 90s is a mother-fashion in which all symbolic mean-ings of the hippie style are weakened and the only outer shape of hippie style is presented and suggested. It doesn't show any cultural backgrounds ideology social value and new attitude on sex of the hippies. The transferal phenomenon of anti-fashion into mother-fashion is summarized as follows. First in the process of tranference to highfashion in 60s and 70s and hippie-look in 90s the resistance of the hippie style is weakened and only suggested through the outer look. Second the hippie style which reappeared in the high fashion and mother fashion in 90s are layered look military uniform mode flower print grunge look romantic look peasant look ethnic look gypsy style handicraft ornaments working suit style handicraft ornaments working suit style and the use of the see-through cloth. Third the fact that the popular and high fashion were influenced by anti fashion in 60s proves filter-up theory in fashion adaption theory. As I examined before the ideology of the hippies are faded in high fashion in 60s-70s and hippie-look in 90s. The symbolic meaning of the hippie style is also weakened and only presented as a fashion trend.

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Study of Child Personality and Kinetic Family Drawing Respondent Characteristic (아동의 성격과 동작성 가족화 반응특성 연구)

  • Kang, Young-Ja;Kim, Yun-Hee
    • Korean Journal of Human Ecology
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    • v.8 no.2
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    • pp.255-273
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    • 1999
  • The purpose of this study was to examine the relationship between characteristics of personality and respondent characteristics of Kinetic Family Drawing for young children. The subjects were 170 children(110 boys and 60 girls). The personal interview contained Personality Characteristic Test for young children(In-Sub Song, 1993) and Kinetic Family Drawing Test(Burns and Kaufman, 1982). Results of the test were analyzed by t-test and ANOVA by SAS program. Results are followings. First, chileren's sex and the general tendency of personal characteristic showed significant difference in the emotional personality among 4 personality characteristics. Girls show more positive tendency than boys in moral, physical, appearance and feature which expressed personal feeling and emotion. Also, girls showed more positive tendency than boys in personal characteristic which showed physical ability. Second, Children's sex and individual characteristic in Kinetic Family Drawing respondent characteristic showed significant difference in own's arm length. Also, Using a rare of paper and chapter 1 of the power among the family showed significant difference in styles and symbols. The boys drew lengther arms compared with their height than the girls. The girls were less complicative, anxious, comparative and aggressive for their family. Third, As a result of the study about the relationship between 4 personal characteristics of children and individual's behavior in Kinetic Family Drawing respondent characteristic, the significant difference is showed in academic personality and social personality had higher completion of their father's feature and drew bigger feet. In socal personality, negative behavior than positive children. Fourth, As a result of the study about the relationship between 4 personal characteristics of children and individual's characteristic, the significant difference were found in academic personality, social personality, family personality and emotional personality. Children with negative academic personality drew longer arms than children with positive academic personality, social personality and family personality. Also, Children with negative emotional personality drew more siblings than children with positive emotional personality. Fifth, The academic personality and the social personality had significant difference in the relationship between 4 personal characteristics of children and dynamics. In social personality, normal children were more tendencious to look at the important person with their mother's direction than positive children. Sixth, In terms of the relationship between 4 personal characteristics of children and mode, academic personality and family personality showed significant difference. Children with negative academic personality used more edge of papers than children with positive academic personality and children with positive academic personality and children with negative family personality fold more papers than children with positive family personality. At last, there were no significant difference between 4 personal characteristics of children and styles as well as symbols.

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Isolation and Characterization of Cathepsin B inhilbitor Produced by Streptomyces luteogriseus KT-10 (Streptomyces luteogriseus KT-10 이 생산하는 Cathepsin B 저해물질의 분리 및 특성)

  • 한길환;김상달
    • Microbiology and Biotechnology Letters
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    • v.29 no.2
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    • pp.84-89
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    • 2001
  • Isolation and Characterization of Cathepsin B inhibitor Produced by Streptomyces luteogriseus KT-IO. Han, Kil~Hwan and Sang~Dal Kim*. Department of Applied Microbiology, Yeungnam Universit}/t Kyongsan 712749, Korea - The cathepsin B inhibitor produced by Streptomyces luteogriseus KT-IO was very stable in heat, acidic and alkaline conditions. The cathepsin B inhibitor was isolated from the extracted fraction of culture broth with butanol, methanol and chloroform subsequently, the inhibitor was purified with following several column chromatography sLlch as DEAE-Sephadex A-25, Sephadex G-15, silica gel 60, Sephadex LH-20, and preparative HPLC. The cathepsin B inhibitor showed positively to detective reaction of ninhydrine, 5% H2S04, iodine, but negatively to the reaction of Ehrlich's reagent, DNS, aniline. The molecular formular of cathepsin B inhibitor was elucidated by JR, lH and 13C-NMR, FAB mass and elemental analyzer. Consequently, it was identified as C4HlI04N6. The cathepsin B inhibitor had the mode of competitive inhibition with the reaction of cathepsin B.

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Analysis on the Assist Characteristics for the Knee Extension Motion of Lower Limb Orthosis Using Muscular Stiffness Force Feedback (근육 강성도 힘 피드백을 이용한 하지 보조기의 무릎 신전 운동 보조 특성 분석)

  • Kim, K.;Kang, S.R.;Jeong, G.Y.;Joo, S.J.;Kim, N.G.;Kwon, T.K.
    • Journal of Biomedical Engineering Research
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    • v.31 no.3
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    • pp.217-226
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    • 2010
  • The lower limb orthosis with a pneumatic rubber actuator, which is intended for the assistance and the enhancement of muscular activities of lower limbs was developed in this study. Compared to other knee extension assistive devices being developed by other researchers, our device is designed especially for the elderly people and intended only for slight assistance so that the subjects can keep their muscular strength. For the effectiveness of system, muscular activities of major muscles in lower limbs during sit-to-stand (STS) and squat motion were measured and analyzed. Subjects were performed the STS and squat motion with and without lower limb orthosis. We made comparison muscular activities between with and without lower limb orthosis. Lower limb orthosis was controlled using muscular stiffness force feedback that is controlled by muscular activities of the measured muscle from force sensor. For analysis of muscular activities, electromyography of the subjects was measured during STS and squat motion, and these were measured using MP 150(BIOPAC Systems, Inc.). Muscles of interest were rectus femoris(RF), vastus lateralis(VL), vastus medialis(VM) and vastus intermedius(VI) muscles in lower limbs of the right side. A biodex dynamometer was used to measure the maximal concentric isokinetic strength of the knee extensors of wearing and not wearing orthosis on right side. The test were performed using the concentric isokinetic mode of test with the velocity set at 60°/s for muscles around the knee joints. The experimental result showed that muscular activities in lower limbs wearing orthosis using muscular stiffness force of a vastus medialis muscle was reduced and knee extension torque of an knee joint wearing lower limb orthosis was increased. With this, we confirmed the effectiveness of the developed lower limb orthosis.

In vitro Acetolactate Synthase Inhibition of LGC-40863 in Rice and Barnyardgrass (시규제초제 LGC-40863의 벼와 피에 대한 Acetolactate synthase 저해 활성)

  • Bae, Y.T.;Lee, J.H.;Koo, S.J.
    • Korean Journal of Weed Science
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    • v.17 no.1
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    • pp.66-70
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    • 1997
  • LGC-40863(proposed common name ; pyribenzoxim), (benzophenone O-[2,6-bis[(4,6-dimethoxy-2-pyrimidinyl)oxy]benzoyl]oxime) is a new rice herbicide being developed by LG Chemical Ltd. The herbicide is highly selective between rice(Oryza sativa L.) and weeds including barnyardgrass (Echinochloa crus-galli(L.) P. Beauv.), and assumed to inhibit acetolactate synthase(ALS ; EC 4.1.3.18) because other structurally related herbicides inhibit the enzyme. To know inhibitory activity and the mode of inhibition of LGC-40863, $I_{50}$(concentration inhibiting ALS activity by 50%) and inhibition kinetics were investigated using ALS extracted from rice and barnyardgrass. $I_{50}$ values of LGC-40863 were 14 and 16mM in rice and barnyardgrass, respectively. In contrast to imazapyr(2-[4,5-dihydro-4-mythyl-4-(1-methylethyl)-5-oxo-1H-imidazol-2-yl]-3-pyridine-carboxylic acid) which showed an uncompetitive inhibition pattern, LGC-40863 was a noncompetitive inhibitor to ALS with respect to pyruvate similar to chlorsulfuron(2-chloro-N-((4-methoxy-6-methyl-l,3,5-triazin-2-yl) aminocarbonyl)benz-enesulfonamide) in both plants.

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Recognition and Experience of Sexual Harassment of Female Teachers in Middle and High School ($\cdot$고등학교 여교사들의 직장내 성희롱에 대한 인식 및 경험)

  • Oh Hee Jin;Koh Hyo Jung
    • Journal of Korean Public Health Nursing
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    • v.17 no.2
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    • pp.238-254
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    • 2003
  • The objective of this study was to provide baseline information useful for developing education programs to prevent sexual harassment in schools. Survey of recognition and experiences of sexual harassment and analysis of factors associated with sexual harassment were conducted. The data were collected using questionnaires from December 9 through 28, 2002. A total of 737 female teachers in Daegu participated in the survey. The data were analyzed with descriptive analysis, ANOVA, and T-test using SPSS /WIN 11.0. The study results were as follows: 1. As to the characteristics related to sexual harassment, $51.3\%$ of the female teachers had no experience of preventive education about sexual harassment, $37.2\%$ of the female teachers perceived that enactment of related laws had little influence on the prevalence of sexual harassment, and $59.8\%$ thought strict punishments are required. For teachers who have experienced sexual harassment in schools, $39.8\%$ thought that they must report to the counseling office or female teachers' meeting. On the other hand, $35.7\%$ answered that they would take passive actions such as talking to their friends or colleagues. 2. As to the mode of sexual harassment, participants regarded physical harassment $(4.06\pm0.78)$ as the prominent forms of sexual harassment, followed by visual harassment $(4.04\pm0.77)$, and verbal harassment $(3.86\pm0.81)$. Among physical harassment. 'touching a body part (shoulder, hip, hand, etc)(4.58) was recognized as prominent forms of physical harassment, while 'blocking one's path or impeding one's movement (3.48)' was recognized as least prominent forms of physical harassment. 3. As to the experiences of sexual harassment, $79.8\%$ of the female teachers had experienced sexual harassment. According to the characteristics of schools. public junior high school $(87.8\%)$, private high school $(83.4\%)$ and bisexual school $(82.5\%)$ had higher rates of sexual harassment. 4. As to the characteristics of experiences in sexual harassment. $65.3\%$ thought that sexual harassment was not serious, but $18.9\%$ thought it was serious and $2.2\%$ thought as very serious. 5. As to the characteristics of sexual harassers, $378.6\%$ were 'colleague teachers' and $8.6\%$ were 'principals and/or vice-principals'. Ninety-three percent of sexual harassers were , male' and $40.2\%$ of them were 'in their 40s'. In most cases. the offender of the sexual harassment was male teachers in the same schools. And more than half of the female teachers replied they had never had any education related to sexual harassment. which means that systematic education is quite urgent.

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Analytical Study on Behavior Characteristic of Shear Friction on Reinforced Concrete Shear Wall-Foundation Interface using High-Strength Reinforcing Bar (고강도 전단철근을 사용한 철근콘크리트 전단벽체-기초계면에서의 전단마찰 거동특성에 대한 해석적 연구)

  • Cheon, Ju-Hyun;Lee, Ki-Ho;Baek, Jang-Woon;Park, Hong-Gun;Shin, Hyun-Mock
    • Journal of the Korea Concrete Institute
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    • v.28 no.4
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    • pp.473-480
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    • 2016
  • The purpose of this study is to provide analytical method to reasonably evaluate the complicated failure behaviors of shear friction of reinforced concrete shear wall specimens using grade 500 MPa high-strength bars. A total of 16 test specimens with a variety of variables such as aspect ratio, friction coefficient of interface in construction joint, reinforcement details, reinforcement ratio in each direction, material properties were selected and the analysis was performed by using a non-linear finite element analysis program (RCAHEST) applying the modified shear friction constitutive equation in interface based on the concrete design code (KCI, 2012) and CEB-FIP Model code 2010. The mean and coefficient of variation for maximum load from the experiment and analysis results was predicted 1.04 and 17% respectively and properly evaluated failure mode and overall behavior characteristic until failure occur. Based on the results, the analysis program that was applied modified shear friction constitutive equation is judged as having a relatively high reliability for the analysis results.