• Title/Summary/Keyword: T-CHO

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$RpoB_{127-135}$ Peptide Derived from Mycobacterium tuberculosis is Processed and Presented to HLA-$A^*0201$ Restricted CD8+ T Cells via an Alternate HLA-I Processing Pathway

  • Cho, Jang-Eun;Cho, Sang-Nae;Cho, Sungae
    • Biomedical Science Letters
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    • v.20 no.4
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    • pp.250-255
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    • 2014
  • Mycobacterium tuberculosis (MTB) resides and replicates inside macrophages. In our previous report, we reported that CD8+ T cell-mediated immune responses specific for the peptide derived from MTB RNA polymerase beta-subunit ($RpoB_{127-135}$) could be induced in TB patients expressing HLA-$A^*0201$ subtype. In order to examine whether $RpoB_{127-135}$ specific CD8+ T cells can recognize MTB infected macrophages in vitro, CD8+ T cell lines specific for $RpoB_{127-135}$ peptide were generated from peripheral blood mononuclear cells (PBMCs) of healthy HLA-$A^*0201$ subjects by in vitro immunization technique. In this study, we observed $RpoB_{127-135}$ specific CD8+ T cells could recognize and destroy macrophages infected with MTB for 2 to 4 days. $RpoB_{127-135}$ specific CD8+ T cell immune response was inducible from PBMC of healthy subjects expressing HLA-$A^*0206$ subtype, one of HLA-A2 supertype members. Next, we investigated the HLA-I processing mechanism of $RpoB_{127-135}$ peptide in MTB infected macrophages. As a result, the presentation of the MTB derived epitope peptide, $RpoB_{127-135}$, to CD8+ T cells was not inhibited by the treatment with brefeldin-A (ER-Golgi transport inhibitor) or lactacystin (proteasome inhibitor), which blocks the classical HLA-I processing pathway. However, $RpoB_{127-135}$ specific CD8+ T cell activity was blocked either by the blocking agent for the endocytosis (cytochalasin D) or by the blocking antibody (W6/32) for HLA-I molecules. Therefore, the $RpoB_{127-135}$ peptide may be processed by accessing the alternate HLA-I processing pathway. Understanding the processing and presentation mechanisms of the MTB derived proteins will help to improve the efficacy of vaccines and the efficiency of therapeutic agents for TB.

Evaluations of Inhomogeneous Shimming in $^1$H MR Spectroscopy (자기공명분광에서 비균질 자장보정에 관한 평가)

  • Choe, Bo-Young;Baik, Hyeon-Man;Suh, Tae-Suk;Lee, Hyoung-Koo;Chun, Heung-Jae;Shim, Kyung-Sub
    • Progress in Medical Physics
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    • v.11 no.1
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    • pp.73-83
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    • 2000
  • In this study, we investigate the effects of poor shimming on quantitative measurement of ratios of metabolite levels by proton magnetic resonance spectroscopy ($^1$H MRS). Coefficient of variation (COV) of metabolite ratios for point resolved spectroscopy (PRESS) and stimulated-echo acquisition mode (STEAM) spectra was evaluated from a phantom containing in vivo levels of metabolites using a conventional whole body 1.5T MR system and conventional acquisition and analysis protocol. A statistical P-value was also calculated from a linear regression for relationship of metabolite ratios. N-acetylaspartate (NAA)/ creatine (Cr) and NAA/ choline (Cho) had low COV values for the long and short TE spectra (29.1 and 27.5%; 23.8 and 12.6 %), whereas Cho/Cr and Cr/Cho had high COV values (50.0 and 68.6 %; 27.5 and 29.3 %). A linear relationship between NAA/Cr and Cho/Cr, and between NAA/Cho and Cr/Cho revealed the statistical significance in the long and short TE spectra, respectively (P < 0.0001 and P < 0.0001; P = 0.015 and P = 0.005). There was no significant relationship between Cho/NAA and Cr/NAA in the measurement (P = 0.159; P = 0.910). The present study suggested that NAA/Cr and NAA/Cho could be useful for data with poor shimming in $^1$H MR spectroscopy. In conclusion, statistical significance of metabolite ratios indicated that the Cr and Cho levels could be interpreted as a significant alteration factor in the long and short TE spectra, and then should be used with care to provide precise metabolite quantification.

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ON FUZZY T2-AXIOMS AND FUZZY COMPACTNESS

  • Cho, Sung Ki;Chung, Dong Gweon
    • Korean Journal of Mathematics
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    • v.6 no.2
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    • pp.155-164
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    • 1998
  • In this paper, the fuzzy $T_2$-axioms due to Hutton and Reilly, Ganguly and Saha and Sinha are characterized by using the notion of fuzzy closure. As consequences, we study the relation between the fuzzy $T_2$-axioms and give some examples which show that the axiom of fuzzy compactness, due to Ganguly and Saha, is not compatible with the fuzzy $T_2$-axioms.

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Memory characteristics of SGOI (Silicon-Germanium-On-Insulator) 1T-DRAM with various Ge mole fractions (Ge 농도에 따른 SGOI (Silicon-Germanium-On-Insulator) 1T-DRAM의 메모리 특성)

  • Oh, Jun-Seok;Kim, Min-Soo;Jung, Jong-Wan;Lee, Young-Hie;Chung, Hong-Bay;Cho, Won-Ju
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2009.06a
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    • pp.99-100
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    • 2009
  • SGOI 1T-DRAM cells with various Ge mole fractions were fabricated and compared to the SOI 1T-DRAM cell. SGOI 1T-DRAM cells have a higher leakage current than SOI 1T-DRAM cell at subthreshold region. The leakage current due to crystalline defects and interface states at Si/SiGe increased with Ge mole. This phenomenon causes sensing margin and the retention time of SGOI 1T-DRAMs decreased with increase of Ge mole fraction.

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The Change of Fraction of T.T.M. and Initial Modulus for PET Tire Cord Fibers with Various Spinning Speed (방사속도에 따른 타이어 코드용 PET섬유의 T.T.M. 분율과 초기탄성계수의 변화)

  • Cho, Hyun Hok;Lee, Kee Hwan;Park, Jong Bum;Kim, Sung Joong;Rhim, Moo San
    • Textile Coloration and Finishing
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    • v.6 no.4
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    • pp.34-39
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    • 1994
  • For the purpose of obtaining high modulus PET tire cord fiber by high spinning speed, the change of initial modulus and taut tie molecules (T.T.M) fraction with the PET tire cord fibers by different spinning speed is investigated. Initial modulus decreased with increasing spinning speed but increased above spinning speed of 1500m/min. Therefore, high modulus PET tire cord fiber may be obtained above spinning speed of 3500m/min. It was found that the initial modulus of fibers depends on fraction of T.T.M.

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A clinical case report of Hyperthyroidism(T3 toxicosis) patient (갑상선기능항진증(T3 중독증) 치험 1례에 대한 보고)

  • Lee, Ju-Ho;Choi, Jung-Sik;Cho, Chung-Sik;Kim, Cheol-Jung
    • Journal of Haehwa Medicine
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    • v.16 no.2
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    • pp.225-228
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    • 2007
  • Objective : This study was designed to evaluate oriental medicine therapy on a hyperthyroidism(T3 toxicosis) patient Methods : The clinical data was analyzed on a patient with hyperthiroidism(T3 toxicosis). This patient was treated with oriental medicine therapy only. After treatment, we analyzed a paient clinical symptom and thyroid function(T3, free T4, TSH) Results : After treatment, improvement was seen in clinical symptom, thyroid function test. conclusion : The study suggests that oriental medicine therapy is effective on the treatment of hyperthyroidism(T3 toxicosis).

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INEQUALITIES FOR THE INTEGRAL MEANS OF HOLOMORPHIC FUNCTIONS IN THE STRONGLY PSEUDOCONVEX DOMAIN

  • CHO, HONG-RAE;LEE, JIN-KEE
    • Communications of the Korean Mathematical Society
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    • v.20 no.2
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    • pp.339-350
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    • 2005
  • We obtain the following two inequalities on a strongly pseudoconvex domain $\Omega\;in\;\mathbb{C}^n\;:\;for\;f\;{\in}\;O(\Omega)$ $$\int_{0}^{{\delta}0}t^{a{\mid}a{\mid}+b}M_p^a(t, D^{a}f)dt\lesssim\int_{0}^{{\delta}0}t^{b}M_p^a(t,\;f)dt\;\int_{O}^{{\delta}O}t_{b}M_p^a(t,\;f)dt\lesssim\sum_{j=0}^{m}\int_{O}^{{\delta}O}t^{am+b}M_{p}^{a}\(t,\;\aleph^{i}f\)dt$$. In [9], Shi proved these results for the unit ball in $\mathbb{C}^n$. These are generalizations of some classical results of Hardy and Littlewood.