• Investigative Magnetic Resonance Imaging
• /
• v.12 no.1
• /
• pp.20-26
• /
• 2008

Purpose : To present the T1 and T2 relaxation times of the major cerebral metabolites at 1.5T and 3.0T and compare those between 1.5T and 3.0T. Materials and Methods : Using the phantom containing N-acetyl aspartate (NAA), Choline (Cho), and Creatine (Cr) at both 1.5T and 3.0T MRI, the T1 relaxation times were calculated from the spectral data obtained with 5000 ms repetition time (TR), 20 ms echo time (TE), and 11 different mixing time (TM)s using STEAM (STimulated Echo-Acquisition Mode) method. The T2 relaxation times were obtained from the spectral data obtained with 3000 ms TR and 5 different TEs using PRESS (Point-RESolved Spectroscopy) method. The T1 and T2 relaxation times obtained at 1.5T were compared with those of 3.0T. Results : The T1 relaxation times of NAA were $2293\;{\pm}\;48\;ms$ at 1.5T and $2559\;{\pm}\;124\;ms$ at 3.0T (11.6% increase at 3.0T). The T1 relaxation times of Cho were $2540\;{\pm}\;57\;ms$ at 1.5T and $2644\;{\pm}\;76\;ms$ at 3.0T (4.1% increase at 3.0T). The T1 relaxation times of Cr were $2543\;{\pm}\;75\;ms$ at 1.5T and $2665\;{\pm}\;94\;ms$ at 3.0T (4.8% increase). The T2 relaxation times of NAA were $526\;{\pm}\;81\;ms$ at 1.5T and $468\;{\pm}\;74\;ms$ at 3.0T (11.0% decrease at 3.0T). The T2 relaxation times of Cho were $220\;{\pm}\;44ms$ at 1.5T and $182\;{\pm}\;35\;ms$ at 3.0T (17.3% decrease at 3.0T). The T2 relaxation times of Cr were $289\;{\pm}\;47\;ms$ at 1.5T and $275\;{\pm}\;57\;ms$ at 3.0T (4.8% decrease at 3.0T). Conclusion : The T1 relaxation times of the major cerebral metabolites (NAA, Cr, Cho), which were measured at the phantom, were 4.1%-11.6% longer at 3.0T than at 1.5T. The T2 relaxation times of them were 4.8%-17.3% shorter at 3.0T than at 1.5T. To optimize MR spectroscopy at 3.0T, TR should be lengthened and TE should be shortened.

• Journal of Microbiology and Biotechnology
• /
• v.16 no.5
• /
• pp.695-703
• /
• 2006

The dissolved oxygen level of any cell culture environment has a critical effect on cellular metabolism. Specifically, hypoxia condition decreases cell viability and recombinant protein productivity. In this work, to develop CHO cells producing recombinant protein with high productivity, mammalian expression vectors containing a human tissue-type plasminogen activator (t-PA) gene with hypoxia response element (HRE) were constructed and stably transfected into CHO cells. CHO/2HRE-t-PA cells produced 2-folds higher recombinant t-PA production than CHO/t-PA cells in a $Ba^{2+}-alginate$ immobilized culture, and 16.8-folds in a repeated batch culture. In a non-aerated batch culture of suspension-adapted cells, t-PA productivity of CHO/2HRE/t-PA cells was 4.2-folds higher than that of CHO/t-PA cells. Our results indicate that HRE is a useful tool for the enhancement of protein productivity in mammalian cell cultures.

• Proceedings of the Korean Radioactive Waste Society Conference
• /
• 2017.10a
• /
• pp.185-186
• /
• 2017

• ETRI Journal
• /
• v.30 no.1
• /
• pp.141-151
• /
• 2008

In this paper, we introduce a new verification platform with ARM- and DSP-based multiprocessor architecture. Its simple communication interface with a crossbar switch architecture is suitable for a heterogeneous multiprocessor platform. The platform is used to verify the function and performance of a DVB-T baseband receiver using hardware and software partitioning techniques with a seamless hardware/software co-verification tool. We present a dual-processor platform with an ARM926 and a Teak DSP, but it cannot satisfy the standard specification of EN 300 744 of DVB-T ETSI. Therefore, we propose a new multiprocessor strategy with an ARM926 and three Teak DSPs synchronized at 166 MHz to satisfy the required specification of DVB-T.

• 한국생물공학회:학술대회논문집
• /
• 2002.04a
• /
• pp.257-260
• /
• 2002

Dissolved oxygen level of cell culture media has a critical effect on cellular metabolism, which governs specific productivity of recombinant proteins and mammalian cell growth However, in the cores of cell aggregates or cell-immobilized beads, oxygen level frequently goes below a critical level. Mammalian cells have a number of genes induced in the lower level of oxygen, and the genes contain a common cis-acting element (-RCGTG-), hypoxia response element (HRE). By binding of hypoxia inducible factor-l (HIF-I) to the HRE, promoters of hypoxia inducible genes are activated, which is a survival mechanism. In this work, to develop a CHO cell capable of producing recombinant proteins in immobilization and high density cell culture efficiently, mammalian expression vectors containing human tissue-type plasminogen activator (t-PA) gene controlled by HRE were constructed and stably transfected into the CHO cells. In $Ba^{2+}$ -alginate immobilization culture, CHO/pCl/dhfr/2HRE-t-PA cells produced 2 folds higher recombinant t-PA activity than CHO/pCl/dhfrlt-PA cells without $CoCl_2$ treatment. Furthermore, in repeated fed batch culture, productivity of t-PA in immobilized CHO/pCI/dhfr/2HRE-t-PA cells was 121 ng/ml/day, total production of 0.968 mg/day at 11 days culture while CHO/pCIIdhfrlt-PA cells was 22.8 ng/ml/day. All these results indicate that HRE is very useful for the enhancement of protein productivity in mammalian cell cultures.

• East Asian mathematical journal
• /
• v.19 no.2
• /
• pp.251-259
• /
• 2003

The concepts of t-rational extensions and t-essential extensions of modules, where t a preradical for R-Mod, are introduced. The structures of such extensions are determined. Relations between maximal t-rational extensions and other concepts of modules are studied.

• 한국생물공학회:학술대회논문집
• /
• 2003.10a
• /
• pp.446-450
• /
• 2003

Maximum adsorption amount of the mixed culture, $2.06{\times}10^{11} cells/g$ is closed to the sum of that of each bacterial component: $1.39{\times}10^{11}cells/g$ for T. ferrooxidans and $1.08{\times}10^{11}cells/g$ for T. thiooxidans. These indicate that the two bacterial species have almost independent preferential adsorption sites on furnace dust. However, the efficiency of leaching, 35% for mixture, 45% for T.f. and 30% for T.t., was not linearly related to the ratio of adsorption in three different systems even though it was increased in parallel with the bacterial adsorption in the same system.

• Communications of the Korean Mathematical Society
• /
• v.14 no.2
• /
• pp.393-403
• /
• 1999

Some fuzzy T\ulcorner-axioms are characterized in terms of the notion of fuzzy closure and the relationship between those fuzzy T\ulcorner-axioms are obtained. Also, finite fuzzy topological spaces satisfying one of those axioms are studied.

• Proceedings of the Korean Institute of Intelligent Systems Conference
• /
• 1996.10a
• /
• pp.66-70
• /
• 1996

We investigate some of algebraic properties of T-generalized state machines, T-generalized transformation semigroups, coverings of T-generalized state machines and T-generalized transformation semigroups.

• Proceedings of the Korean Radioactive Waste Society Conference
• /
• 2017.10a
• /
• pp.265-266
• /
• 2017