• Wind and Structures
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• 제11권1호
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• pp.1-18
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• 2008

Interference effects in five square tall buildings arranged in an L- or T-shaped pattern are investigated in the wind tunnel. Mean and fluctuating shear forces, overturning moments and torsional moment are measured on each building with a force balance mounted at its base. Results are obtained at two values of clear separation between adjacent buildings, at half and a quarter building breadth. It is found that strong interference effect exists on all member buildings, resulting in significant modifications of wind loads as compared with the isolated single building case. Sheltering effect is observed on wind loads acting along the direction of an arm of the "L" or "T" on the inner buildings. However, increase in these wind loads from the isolated single building case is found on the most upwind edge building in the arm when wind blows at a slight oblique angle to the arm. The corner formed by two arms of buildings results in some wind catchment effect leading to increased wind pressure on windward building faces. Interesting interference phenomena such as negative drag force are reported. Interference effects on wind load fluctuations, load spectra and dynamic building responses are also studied and discussed.

• 한국동물생명공학회지
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• 제35권2호
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• pp.155-162
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• 2020

Equine follicle stimulating hormone receptor (eFSHR) has a large extracellular domain and an intracellular domain containing approximately 10 phosphorylation sites within the G protein-coupled receptor. This study was conducted to analyze the function of phosphorylation sties at the eFSHR C-terminal region. We constructed a mutant of eFSHR, in which the C-terminal cytoplasmic tail was truncated at residue 641 (eFSHR-t641). This removed 10 potential phosphorylation sites from the C-terminal region of the intracellular loop. The eFSHR-wild type (eFSHR-wt) and eFSHR-t641 cDNAs were subcloned into the pCMV-ARMS1-PK2 expression vector. These plasmids were transfected into PathHunter CHO-K1 Parental cells expressing β-arrestin 2 enzyme acceptor fusion protein and analyzed for agonist-induced cAMP response. The cAMP response in cells expressing eFSHR-t641 was lower than the response in cells expressing eFSHR-wt. EC₅₀ values of eFSHR-wt and eFSHR-t641 were 1079 ng/mL and 1834 ng/mL, respectively. eFSHR-t641 was approximately 0.58-fold compared with that of eFSHR-wt. The maximal response in eFSHR-wt and eFSHR-t641 was 24.7 nM and 16.7 nM, respectively. The Rmax value of phosphorylation sites in eFSHR-t641 was also decreased to approximately 68.4% of that in eFSHR-wt. The collective data implicate that the phosphorylation sites in the eFSHR C-terminal region have a pivotal role in signal transduction in PathHunter CHO-K1 cells, and indicate that β-arrestin is involved in coupling the activated receptors to the internalization system.

• 근관절건강학회지
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• 제1권1호
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• pp.97-115
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• 1994

The purpose of this study was to examine the reliability and validity of the observed behaviors of subjects who suffered from low back pain with spinal diseases, Thirty two low back pain patients admitted on the neurosurgical unit in an army hospital were compared with 30 normal controls belonged to an army unit, by means of matching the age, hight and weight. Observed pain behaviors were developed by the researcher on the bases of literature and patient observation. This tool consists of 18 behaviors seperated into two major groups : mutually exclusive and concomittent behaviors. The mutually exclusive behaviors included coding cathegories for 6. body motions assumed by the subjects during the observation session. These 6 standardized motions consisted of sitting from standing first, and serially tying down, reclining, sitting again, and then standing, 6 steps walking. Concomittent behaviors consisted of 12 observable patterns that can be observed systematically from the face, grimacing, bracing, rubbing, walking with arms fixed, support with hands on sitting or standing, guarded movement, limping, unbalaced weightbearing, stopped movement from tying position to sitting, sighing and graoning. Subjects were videotrecorded as they performed a 6-standardized sequence of motions, simultanously researcher measured the time spent performing each motion and step length. Patients were asked torate their subjective pain score on the 10 mm graphic rating scale ranging from 'no pain' to 'sever pain'. For scoring of the pain behaviors, two trained nursing officiers independently and simutanously viewwd each videorecording and checked subject 'pain behaviors at the observational item checklist. The result of the study are summarized as follows : 1. Reability of the observational tool was a=.845. 2. Spearman's rho and percentage agreement were p=.97 and 81.7 persent respectively, that indicate adequate interrater reability of this tool. 3. The sensitivity rate of the tool was .875 while specificity rate .866 for differentiating patient from the normal. 4. When difference in the objective pain indices between patient group and control were compared, there was significant difference of all indices, such as pain behavior(t=7.71, p=.0001). spent time performing motion(t=14.2, p=.0001), step length (t=-10.72, p=.0001). 5. There were differences in the objective indices the subjective pain subgroups (low, medium, high). Differences in the mean score of objective pain behavior (F=6.376. p=.005) and spent time for moyion(F=4.631, p=.018). But there were no significant differences in the step length among the subgroups(F=.667, p=.521). 6. Highly correlated pain behavior items wiyh subjective pain score were 'stopped movement from lying position to sitting', 'limping', 'support with hands on sitting or standing', 'bracing', 'guarding' and 'walking with arms fixed'. In summary, although some of rho behavior items such as sighing and groaning in this study could not be observed because of videotaped datd, the reliability and validity of the over all observation method were satifactory. Thus, the results of the present study demonstrate rye potetional utility of the tool in assessing objective pain complementing self-reported pain in low back pain patients.

• 대한근관절건강학회:학술대회논문집
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• 대한근관절건강학회 2001년도 제14회 총회 및 춘계학술대회
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• pp.179-190
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• 2001

The purpose of this study was to explore the effects of GEAP on pain, joint function, activities of daily living(ADL) and fatigue in chronic arthritis patients. The GEAP was held twice a week for 6 weeks for chronic arthritis patients at one university hospital in Seoul, Korea. Thirty four subjects completed the program, who were recruited at four times from September, 1999 to September, 2000. The effect of GEAP were evaluated as follows: Pain severity and number of painful joints ADL, fatigue were measured before and after the GEAP. In order to examine the joint flexibility and strengthening, the followings were measured: the extent of the upward arm reach in both sides(flexibility of shoulder), the ability to touch fingertips of the both hands in back pat and rub(flexibility of arm), the degree of range of motion (ROM) of both ankles in their dorsiflexion(flexibility of ankle) and plantarflexion with standing with toe(strengthening of ankle), and the degree of knee extension, and the grip strength. Paired t-test and Wilcoxon signed rank test were used for data analysis and the significance of the differences in the variables was examined to compare the data obtained before and after the GEAP. After the GEAP, followings were found: 1. Pain severity and number of painful joints was significantly decreased. 2. The flexibility of both shoulders and arms, knee, both ankle were significantly improved. 3. The strengthening of both arms was significantly improved, but the strengthening of ankle was not changed. 4. ADL was significantly increased. 5. Fatigue was significantly decreased. In conclusion, GEAP used in this study was clearly proved to be an effective exercise program to reduce pain and fatigue, to enhance joint function and ADL in people with chronic arthritis. It is suggested that the GEAP should be recommended as one of the useful and appropriate nursing interventions for chronic arthritis patients.

• The Journal of Korean Physical Therapy
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• 제21권1호
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• pp.1-7
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• 2009

Purpose: The aim of this study was to determine the effect of proprioceptive neuromuscular facilitation (PNF) leg patterns on muscle activation of paretic and nonparetic arm in post-stroke hemiparetic subjects. Methods: Eighteen hemiparetic patients participated in this study. Each subject was asked to perform four PNF leg patterns against maximal manual resistance on nonparetic leg. EMG data were collected from biceps and triceps on the paretic and nonparetic side. The measured EMG data was digitized and processed to root mean square (RMS) and expressed as percentage maximal voluntary isometric contraction (％MVIC). The data were analyzed using paired t-test and one-way analysis of variance (ANOVA) with repeated measures to determine the statistical significance. Results: The results of this study were summarized as follows: Firstly, during four PNF patterns application, all of the ％MVIC values of biceps and triceps in paretic and nonparetic arms increased significantly compared with resting condition (p<0.05). Secondly, there was a significant difference in ％MVIC of paretic biceps between PNF leg pattern 1 and 4 (p<0.05). Conclusion: In conclusion, regardless of the kinematic components of PNF leg patterns, all of the muscle activation of biceps and triceps in paretic and nonparetic arm was significantly increased. The finding of this study indicates that irradiation effect caused by PNF leg patterns is beneficial for increased muscle activation of both paretic and nonparetic arms in post-stroke patients.

• Asian Pacific Journal of Cancer Prevention
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• 제14권5호
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• pp.2879-2883
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• 2013

Objective: To investigate differences in mutations of epidermal growth factor receptor (EGFR) gene and relationships with clinicopathological features in patients with non-small cell lung cancer (NSCLC) between Uygur and Han ethnic groups. Methods: The Scorpions amplification refractory mutation system (Scorpions ARMS) was used to measure mutations in exons 18, 19, 20 and 21 of the EGFR gene in paraffin-embedded tumor tissue from NSCLC cases, and statistical analysis was performed to investigate links with clinicopathological features in different histological types of NSCLC. Results: Results from ARMS testing showed EGFR mutations in tumor tissues from six (6) of 50 NSCLC patients of Uygur ethnic group, with a positive rate of 12.0%; four of them (4) had exon 19 deletion in EGFR, and two (2) had L858R point mutation in exon 21 of EGFR. Statistically significant difference was noted in EGFR genetic mutation between adenocarcinoma and non-adenocarcinoma (P < 0.05), but no differences with gender, age group, smoking status, or stage (P > 0.05). EGFR mutations were detected in tumor tissues from 27 of 49 NSCLC patients of Han ethnic group, with a positive rate of 55.1%; 19 of them had exon 19 deletions, seven (7) had L858R point mutations in exon 21 of EGFR and one (1) had mutations in both exon 18 G719X and exon 20 T790M of EGFR. Statistically significant differences were noted in EGFR genetic mutations between genders and between adenocarcinoma and non-adenocarcinoma (P<0.05), but not with age group, smoking status, or stage (P > 0.05). Conclusion: Statistically significant differences were noted in the positive rates of EGFR genetic mutations in NSCLC patients between Uygur and Han ethnic groups, with lower positive rates for the Uygur cases.

• 복식문화연구
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• 제19권1호
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• pp.42-53
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• 2011

The purpose of this study was to investigate donning and doffing independence of the disabled individuals. The subjects of this study were who had kinetic disabilities on upper-limbs. 31 women and 38 children were participated in the survey. They were classified with six groups according to their upper-limbs' kinetic ability levels. Three upper-limbs' kinetic abilities were adopted: Lifting arms up to the chest, twisting shoulder to throw arms toward the back, and buttoning clothes by oneself. The independency of donning and doffing of 14 upper-body garment styles were evaluated by subjects. The donning and doffing independency of 14 garment styles was significantly differentiated by the level of kinetic abilities and garment styles. The person who able to button clothes by oneself could don and doff clothes by oneself. The results also revealed that the independency of donning and doffing was significantly different between cerebral palsy and apoplexy groups. The persons having a stroke of apoplexy were more likely to be able to dress independently than the persons with cerebral palsy. The persons with a paralysed arm were more likely able to wear ready-to-wear clothes of various styles. The donning and doffing independency was also significantly differentiated by the styles of garments. The tight fit style garments were more difficult to be dressed independently than the loose fit style garments. The jacket and shirts, which were buttoned from neck to bottom, were more difficult to be dressed independently than T-shirts.

• IMMUNE NETWORK
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• 제13권6호
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• pp.264-274
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• 2013

The unrestricted population of $CD4^+Foxp3^+$ regulatory T (Treg) cells, which have been known to control the expression of autoimmune diseases and protective immunity to inflammatory reactions, has led to greater appreciation of functional plasticity. Detecting and/or isolating Ag-specific $CD4^+Foxp3^+$ Tregs at the single cell level are required to study their function and plasticity. In this study, we established and compared both MHC class II tetramer and intracellular CD154 staining, in order to detect $CD4^+Foxp3^+$ Treg specific for foreign Ag in acute and chronic infections with lymphocytic choriomeningitis virus (LCMV). Our results revealed that MHC class II tetramer staining showed a lower detection rate of LCMV $GP_{66-77}$-specific $CD4^+$ T cells because most of MHC class II tetramers were unbound and unstable when combined staining was performed with intracellular cytokines. In contrast, intracellular CD154 staining was revealed to be easier and simple for detecting LCMV $GP_{66-77}$-specific $CD4^+$ T cells, compared to MHC class II tetramer staining. Subsequently, we employed intracellular CD154 staining to detect LCMV $GP_{66-77}$-specific $CD4^+Foxp3^+$ Tregs using $Foxp3^{GFP}$ knock-in mouse, and found that LCMV $GP_{66-77}$-specific $CD4^+Foxp3^+$ Tregs and polyclonal $CD4^+Foxp3^+$ Tregs showed differential expansion in mice infected with LCMV Arms or Cl13 at acute (8 and 13 days pi) and chronic phases (35 days pi). Therefore, our results provide insight into the valuable use of intracellular CD154 staining to detect and characterize foreign Ag-specific $CD4^+Foxp3^+$ Treg in various models.

• 여성건강간호학회지
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• 제9권2호
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• pp.113-127
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• 2003

The purpose of this study is to identify the relationship between sexual attitude and risk behaviors, between risk behavior and sexual experiences in Korean girls high school students. The subjects for this study were 522 girls, who were samples from a target population of 63,375 11th grade students from 200 regular high schools and 70 vocational high schools in Seoul. The data were collected from June 22 to July 18, 2002. A structured questionnaire was used that included measurement of general characteristics, sexual attitudes, risk behaviors and sexual experiences. The data were processed with the SAS program, which uses descriptive statistics, t-test, and ANOVA in its an alyses. The results of this study are as follows : 1. The frequency of sexual experience is as follows ; 'holding hands' had the highest percentage, 57.4 7%, followed in order by 'arms around' 50.19%, 'act of embracing' 50.19%, 'kiss' 37.74%, 'french kiss' 30.84%, 'touch breasts' 8.62%, 'touch sexual organs' 3.26%, 'coitus' 2.30%, 'contraception' 0.77%, 'pregnancy' 0.19%, 'abortion' 0.19%, and 'prostitution' 0.19%. 2. The score of conservatives types was significantly different depending upon exposure to pornographic material and drinking. In comparison, the score of permissiveness types was significantly different depending upon exposure to pornographic material, drinking, and running away from home. But the score of pleasure seeking types was not significantly different than that of the risk behaviors types. 3. There was statistically significant difference in the sexual experience depending upon exposure to pornographic material, drinking, smoking, and running away from home. 4. In the relationship between sexual attitude types and sexual experience, there was a negative correlation between the conservatives types and sexual experiences such as 'holding hands', 'arms around', 'act of embracing', 'kiss', 'french kiss', 'touch breasts', 'touch sexual organs', and 'coitus'. There was a positive correlation between the permissiveness types and sexual experiences such as 'holding hands', 'arms around', 'act of embracing', 'kiss', 'french kiss', 'touch breasts', 'touch sexual organs', and 'coitus'. There was also a positive correlation between the pleasure-seeking types and sexual experiences such as 'touch sexual organ', 'contraceptions', 'pregnancy', and 'prostitution'. Based on the finding of this study, this society should develop a integrated program to prevent risk behaviors and sexual experiences in girls high school students.

• Advances in Traditional Medicine
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• 제7권1호
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• pp.41-45
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• 2007

The roots of the plant Korean ginseng have been extensively used in the traditional Chinese herbal medicine. The effects of chronic administration of Korean ginseng extract (KGE) were investigated on two different anxiety models: the elevated T-maze (for inhibitory avoidance and escape measurements) and the open field test (OFT). Diazepam (1 mg/kg), KGE (10, 30 and 100 mg/kg) were administered orally for 15 days. On the 14th day, mice were previously exposed for 30 min to one of the open arms of the T-maze, 24 h before the test. On 15th day, mice had two exposures to the enclosed and open arm of the elevated T-maze followed by exposure to the open field apparatus. The number of line crossings in the apparatus was used to assess locomotor changes. Cumulative Concentration Response Curve of 5-HT was plotted using rat fundus which were pre-treated in a similar way. Treatment with Diazepam (1 mg/kg) and KGE (10, 30 and 100 mg/kg) significantly (P < 0.05) impaired inhibitory avoidance performance but did not impair escape latency. In OFT, diazepam facilitated locomotion as compared to vehicle and other treatment groups. KGE at any of the selected doses did not impair locomotion. Concentration response curve of 5-HT was shifted towards the right with suppression of maxima in rats treated with KGE. The results suggest that KGE exerts anxiolytic like behaviour in a specific subset of defensive behaviour, particularly those related to generalized anxiety disorder.