• Journal of environmental and Sanitary engineering
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• v.19 no.2
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• pp.15-22
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• 2004

Yellow loess, Tidal plat sediment, and mud are blown to be high in adsorption though they have great distinction according to the ratio of clay minerals content and their kinds. Thus one of the samples for this study can be obtained by drying of Yellow loess, Tidal plat sediment, and the mud which is semifinished products that are from the progress of heat treatment and making homogeneity which can be found everywhere in our county. Another sample can be prepared by reducing pollutants with the adsorbent and by homing heavy metals of their on. With the samples, noxious heavy metals such as Pb, Cd, and essential trace elements such as Cu, Fe, Mn by each concentration is tested for adsorption according to pH, the kinds of adsorbents and the amount of injection. With the help of these steps, this study shows that the adsorbents, which are green, reducing the additional pollution and low in price, can be found. It also shows that the optimal condition for removing pollutants can be found and the basic materials for treating water can be offered. The results were as follows ; Yellow loess shows the rates of adsorption by more than 50% and Tidal plat sediment and Mud show it by more than 90%. Thus Tidal plat sediment and Mud are higher than Yellow loess the rates of adsorption. The pH should be treated in natural condition because the rates of adsorption of Pb, Cd, Cu, Mn excepting Fe in mud is higher in pH 7 than in pH 3 of Yellow loess, Tidal plat sediment, and Mud. The drying adsorbents are good to use because the rates of adsorption of heavy metals has tendency to be higher in the drying method than in the burning method. It is considered that the more the mont of the injection of the adsorbent is , the higher the rates of adsorption is, and one gram is reasonable for the amount of the injection of Tidal plat sediment and mud. Yellow loess is suitable for the treatment of the water that includes low concentration of heavy metals because it has the lower rate of adsorption as the concentration of noxious heavy metals is higher. It is thought that Tidal plat sediment and Mud is proper for the treatment of the water that includes high concentration of heavy metals because the rates of adsorption has not been changed as the concentration of heavy metals increases.

• Journal of Soil and Groundwater Environment
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• v.17 no.3
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• pp.21-31
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• 2012

This paper presents the short-term and long-term measures to determine the fugitive dust concentration in a contaminated site, which is a crucial step for the determination of particulate emission factor (PEF) for risk assessment. As a long-term measure, USEPA method employing Q/C value (inverse of the ratio of the geometric mean air concentration to the emission flux at center of a 0.5-acre square source) seems to be suitable as it reflects regional-specific meteorological conditions. However, it requires nation-wide database collection and interpretation. Use of ASTM method is an alternative as a short-term measure. The method is readily field-applicable as PEF calculation equation is simple and input parameters can be easily derived at the site of interest as well without the nation-wide efforts. Using ASTM method, PEF at the Former Janghang Smelter Site was determined. According to various mode of aggregate size distribution and fractions of vegetative cover, which are the most important factors in PEF calculation, PEF values at the Former Janghang Smelter Site varied greatly. When the mode of aggregate size distribution was set at 0.25 mm, PEF values at the Former Janghang Smelter Site was 5~20 times higher than the default PEF value (i.e., 35 ${\mu}g/m^3$) shown in the current Korean Soil Contamination Risk Assessment Guidance. On contrast, when the mode was set at 2 mm, PEF values at the Former Janghang Smelter Site was 160~640 times lower than the default PEF value in the Korean Guidance.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.10
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• pp.1656-1665
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• 2020

Objective: This study aimed to determine the effects of stress during slaughter of beef cattle on physiological parameters, carcass, and meat quality at a Federal Inspection Type slaughterhouse located in the southeast of Mexico. Methods: A total of 448 carcasses of male Zebu×European steers with an average age of 36 months were included. Carcass assessment of presence of bruises and bruise characteristics was carried out on each half-carcass. Blood variable indicators of stress (packed cell volume, neutrophil to lymphocyte ratio, glucose, cortisol concentration) and meat quality parameters (pH, color, shear force, drip loss) were evaluated. Results: Of the 448 carcasses evaluated, 81% of the carcasses showed at least one bruise; one bruise was detected in 36.6% and two bruises in 27.0% of animals. Of the 775 bruises found, 69.2% of the bruises were grade 1 in region 3. Of the 448 carcasses studied, 69.6% showed hyperglycemia (6.91 mmol/L); 44.3% and 22.7% showed high (74.7 ng/mL) and extremely high (108.8 ng/mL) cortisol levels, respectively, indicative of inadequate handling of animals during preslaughter and slaughter. Of the carcasses evaluated, 90.4% had a pH ≥5.8 with an average of pH 6.3. In both pH groups, meat samples showed L^⋆ values >37.0 (81.6%) and a shear force >54.3 N; meat pH≥5.8 group showed a drip loss of 2.5%. These findings were indicative of dark, firm, and dry (DFD) meat. According to principal component analysis, grades 1 and 2 bruises in region 3 and grade 1 bruises in region 5 were highly associated with cortisol, drip loss, and color parameters b^⋆ and h^⋆ and were negatively associated with L^⋆, a^⋆, and C^⋆. Conclusion: The bruises probably caused by stress-inducing situations triggered DFD meat. Appropriate changes in handling routines in operating conditions should be made to minimize stress to animals during the slaughter process to improve animal welfare and meat quality.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.12
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• pp.5069-5073
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• 2015

The single nucleotide polymorphism (SNP) rs1053004 in Signal transducer and activator of transcription 3 (STAT3) was recently reported to be associated with chronic hepatitis B (CHB)-related hepatocellular carcinoma (HCC) in a Chinese cohort. This study was aimed at investigating whether the SNP might also contribute to HCC susceptibility in the Thai population. Study subjects were enrolled and divided into 3 groups including CHB-related HCC (n=211), CHB without HCC (n=233) and healthy controls (n=206). The SNP was genotyped using allelic discrimination assays based on TaqMan real-time PCR. Data analysis revealed that the distribution of different genotypes was in Hardy-Weinberg equilibrium (P>0.05). The frequencies of allele T (major allele) in HCC patients, CHB patients and healthy controls were 51.4%, 58.6% and 61.4%, respectively, whereas the frequencies of C allele (minor allele) were 48.6%, 41.4% and 38.6%. The C allele frequency was higher in HCC when compared with CHB patients (odds ratio (OR)=1.34, 95% confidence interval (CI)=1.02-1.74, P=0.032). The genotype of SNP rs1053004 (CC versus TT+TC) was significantly associated with an increased risk when compared with CHB patients (OR=1.83, 95% CI=1.13-2.99, P=0.015). In addition, we observed a similar trend of association when comparing HCC patients with healthy controls (OR=1.77, 95% CI=1.07-2.93, P=0.025) and all controls (OR=1.81, 95% CI=1.19-2.74, P=0.005). These findings suggest that the SNP rs1053004 in STAT3 might contribute to HCC susceptibility and could be used as a genetic marker for HCC in the Thai population.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.5
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• pp.2101-2107
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• 2014

Background: Single nucleotide polymorphisms (SNPs) affecting microRNA (miR) sequences may influence carcinogenesis. Our current study primarily aimed to confirm previously conducted association studies between rs2910164 found on miR-146a, and rs11614913 located on miR-196a2 polymorphisms and cancer phenotypes in the Japanese elderly population. rs2910164 (G/C) and rs11614913 (T/C) polymorphisms were determined by genotyping on the samples collected from 1,351 consecutive autopsy cases registered in the Japanese SNPs for geriatric research (JG-SNP) data base. Cancer samples were systematically reviewed, pathologically verified and assessed with respect to miR-146a and miR-196a2 genotypic variation. The current study covered 726 males and 625 females with a mean age of $80.3{\pm}8.9$ years. The study included 524 subjects without cancer and 827 subjects with at least one type of cancer, such as gastric (n=160), lung (n=148), colorectal (n=116) or others. Males with cancers (n=467) were more numerous than females (n=360). Both rs11614913 (CT: TT adjusted odds ratio (OR) 95% confidence interval (95%CI)=0.98 (0.75-1.28), p=0.873, CC: TT adjusted OR (95%CI)=1.06 (0.76-1.47), p=0.737, CT+CC: TT, adjusted OR (95%CI)=0.99 (0.77-1.29), p=0.990), and rs2910164 (CG: CC adjusted OR (95%CI)=1.12 (0.87-1.44), p=0.383, GG: CC adjusted OR (95%CI)=1.03 (0.71-1.48), p=0.887, CG+GG: CC adjusted OR (95%CI)=1.10 (0.87-1.39), p=0.446) polymorphisms did not show significant association with overall cancer in all subjects. However, "CC" genotype in rs11614913 polymorphism was significantly associated with increased gastric cancer (n=160) in all subjects (CC: CT+TT, adjusted OR (95%CI)=1.50 (1.02-2.22), p=0.040). We found that rs11614913 and rs2910164 do not pose general cancer risk, but rs11614913 may influence gastric cancer in Japanese elderly population. Confirmation of our study results requires further investigations with larger subject populations.

• BMB Reports
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• v.35 no.3
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• pp.313-319
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• 2002

N-Acetyl-$\beta$-D-hexosaminidase ($\beta$-HexNAc'ase) (EC 3.2.1.52) was purified from rice seeds (Oryza sative L. var. Dongjin) using ammonium sulfate (80%) precipitation, Sephadex G-150, CM-Sephadex, and DEAE-Sephadex chromatography, sequentially. The activities were separated into 7 fractions($F_1-F_7$) by CM-Sephadex chromatography. Among them, F6 was further purified to homogeneity with a 13.0% yield and 123.3 purification-fold. The molecular mass was estimated to be about 52 kDa on SDS-PAGE and 37.4 kDa on Sephacryl S-300 gel filtration. The enzyme catalyzed the hydrolysis of both p-nitrophenyl-N-acetyl-$\beta$-D-hexosaminide (pNP-GlcNAc) and p-nitrophenyl-N-acetyl-$\beta$-D-hexosaminide (pNP-GalNAc) as substrates, which are typical properties of $\beta$-HexNAc'ase. The ratio of the pNP-GlcNAc'ase activity to the pNP-GalNAc'ase activity was 4.0. However, it could not hydrolyze chitin, chitosan, pNP-$\beta$-glucopyranoside, or pNP-$\beta$-glucopyranoside. The enzyme showed $K_m$, $V_{max}$ and $K_{cat}$ for pNP-GlcNAc of 1.65 mM, $79.49\;mM\;min^{-1}$, and $4.79{\times}10^6\;min^{-1}$, respectively. The comparison of kinetic values for pNP-GlcNAc and pNP-GalNAc revealed that the two enzyme activities are associated with a single binding site. The purified enzyme exhibited optimum pH and temperature for pNP-GlcNAc of 5.0 and $50^{\circ}C$, respectively. The enzyme activity for pNP-GlcNAc was stable at pH 5.0-5.5 and $20-40^{\circ}C$. The enzyme activity was completely inhibited at a concentration of 0.1 mM $HgCl_$ and $AgNO_3$, suggesting that the intact thiol group is essential for activity. Chloramine T completely inhibited the activity, indicating the possible involvement of methionines in the mechanism of the enzyme.

• Korean Journal of Adult Nursing
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• v.13 no.1
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• pp.40-52
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• 2001

The ratio of the elderly population is rapidly growing due to socio-economic development and better medical services. As the average life-span of the Korean elderly becomes longer, the proportion of the elderly is increasing. Therefore, the elderly need to sustain a high quality of life. However, all these factors, such as sensory loss, decreased endurance, mobility limitation, and a loss of independence, act to diminish the quality of life among the elderly. It is necessary for elders to adapt themselves to aging, to maintain their physical functions and especially, it is important to keep their independence in activity of daily living and self care. Therefore, this study was designed to identify the degree of activity in daily living and exercise of a self-care agency and to investigate the relationship between activity of daily living and self-care agency. Also, this research aimed to contribute to nursing interventions that maintain and promote quality of life of the elderly and to enable themselves to live independently. The subject of this study were 160 elders, over age 65, who were sampled in C city. Data were collected by observation and questionnaires from May,1999 to December, 1999. The measurement tools were Barthel Index(1979), Lawton & Brody(1969) for activity of daily living and Kearney & Fleischer(1979) for self-care agency. Data were analyzed by SAS PC+ package and included descriptive analysis, t-test, ANOVA, Pearson correlation coefficient, and Cronbach's alpha. The conclusions of this study are summarized as follows; 1. The mean of ADL and IADL was 96.09 and 16.38, respectively. The score described activity of daily living among the elders in C province as moderate. Particularly, in the items of IADL, the item of medication was the highest score. 2. The mean for exercise of self-care agency was 76.48, a moderate degree. 3. The degree of ADL and IADL according to demographic characteristics was significantly different statistically in pocket money, the presence of disease, perceived health status and in occupation, pocket money, family type, the presence of disease, and perceived health status, respectively. 4. The dagree of selt-care agency according to demographic characteristics was significantly different statistically in gender, education level, economic status, and pocket money. 5. There was a significant positive correlation between activity in daily living and self-care agency. Based on these results, further research is suggested to discover the variables influencing the activity of daily living and self-care agency to develop nursing strategies for improving self-care and activity of daily living among the elderly.

• Journal of the Korea Concrete Institute
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• v.18 no.4 s.94
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• pp.489-496
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• 2006

Graft copolymerized polycarboxylate(PC)-type superplasticizers which have carboxylic acid with $\pi$ bond among the molecular structure and polyethyleneglycol methyl ether methacrylate(PMEM) were synthesized by free radical reaction. To investigate their chemical structures and molecular weights, PCs were analyzed by FT-IR(fourier transform spectrometer), C-NMR(nuclear magnetic resonance spectrometer) and GPC(gel permeation chromatograpy). When types of carboxylic acids(methacrylic acid, acrylic acid, maleic anhydride, and itaconic acid) and molar ratios of carboxylic acid/PMEM) were varied, adsorptive and fluid characteristics in cement paste were discussed. As the molar ratio of carboxylic acid/PMEM) was higher, amount adsorbed on the cement particles and the fluidity of cement paste by mini-slump spread testing method were increased. When main chain of PC was methacrylic acid, a larger amount was adsorbed on the cement particles. PCs with acrylic acid as main chain showed higher dispersing power. However, it was confirmed that PCs with dicarboxylic acids(maleic anhydride, itaconic acid) didn't have good adsorption and dispersibility.

• Korean Journal of Environmental Agriculture
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• v.17 no.1
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• pp.65-69
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• 1998

In order to evaluate the utility of bituminous coal fly ash, gypsum, oyster shell as soil amendments, aadic clayloam paddy soils with low calaum content were amended in the upper 15㎝ with amendments, and then Chinese cabbage was cultivated under plastic film house. Amendments treated were, in metric tons per hectare, i) none(Check) ; ii) 80 fly ash(FA) ; iii) 4 shell(SH) ; iv) 56 fly ash+24 gypsum (FG) ; v) 40 fly ash+24 gypsum+0.8 shell(FGS). On the whole, soil chemical properties were improved by amendments treatments. Amongst treatments, FA prominently neutralized soil pH and increased contents of Av. $P_2O_5$, Ex. K, and Av. B in soils. Besides, it showed the highest ratio in bacteria/fungi and (bacteria+actinomycetes)/fungi. FGS also affected the neutralization of soil pH and the increment of Ex. Mg. Amendments plants appeared alkaline damages only at early growing stage, but showed positive responses in fresh weight yields : 23% for FGS : 21% for FG : 19 18% for SH. At harvesting, leaves both of FA and FGS plants had higher values in contents of N, P, K, Ca, Mg, Fe, Mn, Zn, B, reduang-sugar, and vitamin-C than of others. In especial, Check plants appeared the heart rot symptoms owing to calaum defiaency differently from amendments plants. Taken together, FGS was an effective combination enable to maximize the utility of fly ash, gypsum, shell as soil amendments, espeaally in cabbage yield and quality.

• Korean Journal of Animal Reproduction
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• v.25 no.4
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• pp.371-379
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• 2001

The objective of this study was to establish an effective cryopreservation method of in vitro-produced bovine embryos. For the vitrification, in virtro-produced embryos at 8-cell, morula and blastocyst stages were exposed to freezing solution containing 5.5 M EG (EG 5.5) for 20 sec, loaded on each containers such as EM grid, OPS and Cryo-loop, and then immediately plunged into liquid nitrogen at -196$^{\circ}C$. Thawed embryos were serially diluted in 0.5, 0.25 and 0.125 M sucrose in m-HPBS, each for 1 min, and cultured in CRlaa medium supplemented with 10% FBS. Significant differences in the rates of re-expanded and hatched embryos were not observed among these embryo containers. The total cell number of expanded blastocyst cultured in vitro after vitrification was examined by Hoechst staining. There were no differences between non-vitrified (180.0 $\pm$ 5.4) and vitrified groups (178.0 $\pm$ 7.5). In addition, when the cellular injuries after vitrification were compared by double staining. There were no significant difference in the ratio of live and dead cells between non-vitrified group (176 : 4) and vitrified group (172 : 6). Therefore, these results suggest that bovine embryos can be cryopreserved easily, effectively and successfully by vitrification using various containers, such as EM grid, OPS or Cryo-loop in the presence of EG 5.5 freezing solution.