• 한국생물공학회:학술대회논문집
• /
• 2003.04a
• /
• pp.398-401
• /
• 2003

Bacillus species predominantly outgrown in a night soil treatment system were isolated and characterized. Cell interactions took place among them and cell population changed under various culture conditions. Maximum removal of $NH_4\;^+-N$ and cell production occurred under the conditions of 30% DO and C/N ratio of 8. Additions of 0.8% peptone and 0.3% yeast extract to a basal medium influenced the growth of isolates and the removal of $NH_4\;^+-N$ in flask culture, and metal ions such as Ca, Fe and Mg also did. During the flask experiment of nitrogen removal under an aerobic condition, active nitrification by the isolates occurred largely in 1 h with the decrease of COD and alkalinity destructed was only 74.6% of theoretical value. From the nitrogen balance, the percentage of nitrogen lost in the flask culture was estimated to be 29.0%. This conversion of ammonia to $N_2$ under an aerobic condition was confirmed by GC analysis. The B3 process using the Bacillus species seemed to have some economic advantage.

• BMB Reports
• /
• v.31 no.3
• /
• pp.227-232
• /
• 1998

The leukocyte NADPH oxidase of neutrophils is a membrane-bound enzyme that catalyzes the production of $O_2^-$ from oxygen using NADPH as an electron donor. Dormant in resting neutrophils, the enzyme acquires catalytic activity when the cells are exposed to appropriate stimuli. During activation, the cytosolic oxidase components $p47^{phox}$ and $p67^{phox}$ migrate to the plasma membrane, where they associate with cytochrome $b_{558}$, a membrane-bound flavohemoprotein, to assemble the active oxidase. The oxidase can be activated in a cell-free system; the activating agent usually employed is an anionic amphiphile such as sodium dodecyl sulfate (SDS). Because $p47^{phox}$ can translocate by itself during activation, the conformational change in $p47^{phox}$ may be responsible for the activation of NADPH oxidase. We show here that the treatment of $p47^{phox}$ with SDS leads to an increase in the reactivity of the sutbydryl group of cysteines toward N-ethylmaleimide, indicating that the conformational change occurs when $p47^{phox}$ is exposed to SDS. We propose that this change in conformation results in the appearance of a binding site through which $p47^{phox}$ interacts with cytochrome $b_{558}$during the activation process.

• BMB Reports
• /
• v.53 no.2
• /
• pp.65-73
• /
• 2020

Life expectancy has dramatically increased around the world over the last few decades, and staying healthier longer, without chronic disease, has become an important issue. Although understanding aging is a grand challenge, our understanding of the mechanisms underlying the degeneration of cell and tissue functions with age and its contribution to chronic disease has greatly advanced during the past decade. As our immune system alters with aging, abnormal activation of immune cells leads to imbalance of innate and adaptive immunity and develops a persistent and mild systemic inflammation, inflammaging. With their unique therapeutic properties, such as immunomodulation and tissue regeneration, mesenchymal stem cells (MSCs) have been considered to be a promising source for treating autoimmune disease or as anti-aging therapy. Although direct evidence of the role of MSCs in inflammaging has not been thoroughly studied, features reported in senescent MSCs or the aging process of MSCs are associated with inflammaging; MSC niche-driven skewing of hematopoiesis toward the myeloid lineage or oncogenesis, production of pro-inflammatory cytokines, and weakening their modulative property on macrophage polarization, which plays a central role on inflammaging development. This review explores the role of senescent MSCs as an important regulator for onset and progression of inflammaging and as an effective target for anti-aging strategies.

• Journal of the Korean Society of Manufacturing Technology Engineers
• /
• v.22 no.3
• /
• pp.459-464
• /
• 2013

The purpose of a micro-mixing device is to enhance the mixing by increasing the diffusion effect between different types of flows. There have been many attempts to actively or passively increase mixing. However, those studies were limited to lab-scale experiments because the production of devices requires a series of processes, time, cost, and the mixing quality itself. For this reason, this study attempted to develop a quick and simple process for micro-mixing device fabrication by using conventional machining and bonding processes and applying ultrasonic waves from the outside of the mixing device. The mixing quality was quantified by using the mixing index, and the results showed that the proposed system increases the mixing from ~33% to ~10% with respect to the flow rates.

• Archives of design research
• /
• v.17 no.2
• /
• pp.67-76
• /
• 2004

The main stream of consumption about the products is tend to transfer from low price value with mass production, to the second value of products, such as pleasure, familiarity, humor, and metaphor. This second value is closely connected to a redundancy which are also related to modern science as well as product design. These terms such as matrials, identity, and gravitation, disappeared gradually, and these are substituted with an uncertainty principle, a fortuity, a contradictory concept, entropy etc. In sum this study focuses on the tendency of intelligency issue the recent design trends. This result shows that The 4th Column Thinking and Formation System which is related to an expansion and diversity of design intellectual ideas.

• Journal of Biosystems Engineering
• /
• v.29 no.5 s.106
• /
• pp.433-440
• /
• 2004

Worldwide consumption of vegetable soybean has been increasing recently, but in the process of vegetable soybean production, threshing and separation work accounts fur about $80\%$ of overall labor. Therefore, developing of the vegetable soybean thresher is necessary to reduce the cost of labor. The main objective of this study is to acquire the basic in-formations for design of the vegetable soybean thresher which is suitable for domestic circumstances. We made the experimental system to investigate the threshing and separating performance at the several speeds of threshing cylinder and separating blower according to the kinds of threshing tooth. The result are as follows; Threshing performance of vegetable soybean thresher was shown as the best in case the threshing tooth made of rubber which has the 80 of Shore hardness was used at the circumference speed of cylinder of 5.8$\~$8.1m/s. Also separating cleaning performance of vegetable soybean thresher was shown as the best at more than 1,300 rpm of blower speed and $60\~80\%$ of opening ratio of suction port.

• Journal of the Korean Society for Precision Engineering
• /
• v.27 no.4
• /
• pp.63-71
• /
• 2010

Modular design is a very important design methodology that allows designers to develop a product family at low product development and production cost. This design methodology is also very powerful for products that require frequent design changes due to customer requirements. Most research on modular design is focused on the modularization based on functional decomposition, physical interface and manufacturing process of parts. In this paper, we propose a modularization method that takes size of human body parts into consideration for products which have physical interactions with the products such as office chairs and sport machines. Evaluation of modular design is based on dependence measurement between every pair of components in the design. In addition we proposed a module sizing method for the maximization of customer satisfaction in MC(Mass Customization) environment.

• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 2005.06a
• /
• pp.1079-1082
• /
• 2005

This paper deals with the algorithm development that inspects defects such as Lens Focus, Focus check, Black Defect, Dark Defect, Dim Defect, Color Defect, and Line Defect, Angle Defect, IrisAgc Defect caused by the process of 2M Class Compact Camera Module (CCM). Domestic market was majorly comprised of VGA(0.3 million pixel) market. But in the middle of year 2004, camera phone with Mega Pixel has appeared, and it is estimated that the camera phone with Mega Pixel will take up to 28% of total phone sales if it is released in the end of year 2004. Since the inspection of finished products is done manually, it is major obstacle in production increment In this paper, to solve these problems, we developed the imaging processing algorithm to inspect the defects in captured image of assembled CCM. The performances of the developed inspection system and we can recognize various types of defect of CCM modules with good accuracy and high speed

• Journal of the Korean Society for Precision Engineering
• /
• v.32 no.12
• /
• pp.1011-1016
• /
• 2015

In a roll-to-roll continuous system, winding is one of the most important processes since it determines the quality of the final manufactured products such as flexible film and printed electronic devices. Since an adequate winding tension can reduce the incidence of the defects that are derived from the inner stress of the wound roll such as starring and telescoping, it is necessary to determine the optimal taper-tension profile. In this study, an algorithm for the setting of an optimal taper-tension profile in consideration of the residual stress in the wound roll is suggested; furthermore, the algorithm was adjusted for the determination of an optimal taper-tension profile regarding the winding process of $10{\mu}m$ polypropylene (PP) film. As a result of the algorithm-generated, optimal taper-tension profile, the residual stress and radial stress in a PP wound roll were decreased to 27.37 % and 40.05 % (mean value), respectively.

• Journal of Korean Society of Industrial and Systems Engineering
• /
• v.33 no.4
• /
• pp.159-166
• /
• 2010

The speed at which products are developed and released to market is tightly linked to profitability and market share. Hence, many companies are still in a desperate need of real Rapid Tooling (RT) technologies which can really help to expedite their prototype tooling and pre -production tooling for injection molding. Many other companies that have been very skeptical of RT technologies developed so far are working on Hybrid Tooling (HT) that can really meet the market standards. With the conviction that HT can be a reliable alternative for current RT technologies, this paper describes the experimentation how HT process has been being successfully established and effectively applied with typical case studies. Through the experimentation, Ceramic-filled SLA tooling was found to be aptly suited for the low grade mold, and Metal SLS tooling was found to be aptly suited for the medium volume mold both in terms of the lead time, dimensional accuracy, and tooling cost.