• 대한기계학회:학술대회논문집
• /
• 대한기계학회 2007년도 춘계학술대회A
• /
• pp.395-398
• /
• 2007

In nature, the swallowtail butterfly is known to be a versatile flyer using gliding and flapping efficiently. Furthermore, it has long tails on the hind-wing that may be associated with the enhancement of the gliding performance. In the present study, we investigate the aerodynamic property of swallowtail butterfly wing in gliding. We use an immersed boundary method and conduct a numerical simulation at the Reynolds numbers of 1,000 - 3,000 based on the free-stream velocity and the averaged chord length for seven different attack angles. As a result, we clearly identify the existence of the wing-tip and leading-edge vortices, and a pair of the streamwise vortices generated along the hind-wing tails. Interestingly, at the attack angle of $10^{\circ},$ hairpin vortices are generated above the center of the body and travel downstream.

• 농촌지도와개발
• /
• 제26권3호
• /
• pp.153-163
• /
• 2019

The study aims to examine the psychological effects of insect-based experience activities. A sample of 167 elementary students was hired for the study. Students in the experimental group participated in insect-based experience activities using swallowtail butterfly. Collected data are analyzed with analysis of variance and paired-sample T-test in SPSS 25.0. The results of this study are as follows 1) Insect based experience activities show positive effects in psychological and physiological aspects, 2) and students and teacher showed high satisfaction in insect-based program. In conclusion, this study contributes to providing fundamental information for the development of agro-healing programs using insect and design guidelines for identifying the characteristics of each healing program. Further research should be extended to different types of student and the development of diverse insect-based activities.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제28권1호
• /
• pp.1-4
• /
• 2014

The effect of an artificial diet on developmental rate, a life history parameter, was examined for the swallowtail butterfly Papilio xuthus. Artificial insect diets are an essential component of many insect rearing systems that produce insects for research purposes. Complex agar-gelled diets are generally prepared in large batches and used shortly after preparation because the degradation of perishable diet ingredients, such as vitamins and fatty acids, can adversely affect insect quality (Brewer 1984). However, the timing of diet preparation may be inconvenient, and large batches wasteful, if the unused excess is discarded. The percentage of pupation varied considerably, with no significant differences among diets, on which a maximum pupation percentage of 83% was observed. Pellet-type diets were investigated with the aim of developing a more easily prepared diet. The extrusion of the artificial diet under high temperature and pressure may induce desirable chemical and physical changes in the extruded product. The purpose of the present study was to develop an artificial diet for rearing P. xuthus.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제29권2호
• /
• pp.153-161
• /
• 2014

The aim of this study is to investigate the effects of ambient thermal environments on the development of swallowtail butterflies (Sericinus montela Gray). Developmental durations and survival rates of S. montela were examined at two crucial developmental stages, embryonic and larval development, at varying temperatures ranging from $15^{\circ}C$ to $35^{\circ}C$. As expected, our results indicated that increasing temperatures decreased the developmental duration and survival rate of the eggs. However, the larvae and pupae showed maximum survival rates at $20.0^{\circ}C$ and $25.0^{\circ}C$, and the represented durations were similar to those of the eggs. Larval development was stage-specific, revealing that the fourth and fifth instars at the later stages were more susceptible to temperature variation. When considering both parameters, the optimal development of S. montela occurred within the temperature range of $20.0-25.0^{\circ}C$. The lower threshold for the complete development of S. montela from eggs to eclosion of adults was calculated at $10.6^{\circ}C$ by linear regression analysis. The estimated value is similar to that of other endemic insects distributed in temperate climate zones, which indicates that S. montela belongs to a small group of swallowtails adjusted to low ambient temperatures. From the results, we predict that the full development of S. montela could be achieved within the temperature range of $17.5-30.0^{\circ}C$. Embryonic development ceased at both test temperature extremes, and no further larval development proceeded after the third instar at $35.0^{\circ}C$. These results suggest that embryogenesis can be significantly influenced by slight variations in the ambient thermal environment that fall below the optimal range.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제31권2호
• /
• pp.79-84
• /
• 2015

A new cecropin-like antimicrobial peptide (Px-CLP) gene was isolated from the immunechallenged larvae of the swallowtail butterfly, Papilio xuthus, by employing annealing control primer (ACP)-based GeneFishing PCR. The full-length cDNA of Px-CLP is 310 nucleotides encoding a 70 amino acid precursor that contains a putative 22-residue signal peptide, a 4-residue propeptide, a presumed 37-residue mature peptide, and an uncommon 7-residue acidic pro-region at the C-terminus. The deduced amino acid sequence of Px-CLP showed significant identities with other Lepidopteran cecropin D type peptides. RT-PCR revealed that the Px-CLP transcript was detected at significant level after injection with bacterial lipopolysaccharide (LPS). The peptides with or without C-terminal acidic sequence region were synthesized on-solid phage and submitted to antibacterial activity assay. The synthetic 37-mer peptide (Px-CLPa), which removed C-terminal acidic sequence region, was showed exclusively antibacterial activity against E. coli ML35; meanwhile, a 44-mer peptide (Px-CLPb) with C-terminal acidic peptide region was not active. This result suggests that Px-CLP is produced as a larger precursor containing a C-terminal pro-region that is subsequently removed by C-terminal modification.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제23권2호
• /
• pp.231-238
• /
• 2011

Attacin is an insect antibacterial protein that plays an important role in immune response to injury and infection. In this report, we have isolated and characterized of cDNA encoding for the attacin from the immunized larvae of swallowtail butterfly, $Papilio$ $xuthus$. A full length cDNA of $P.$ $xuthus$ attacin was obtained by employing annealing control primer (ACP)-based differential display PCR and 5' RACE. The complete $P.$ $xuthus$ attacin cDNA was comprised of 949 bp encoding a 250 amino acid precursor. It contains a putative 18 amino acid signal peptide sequence, a 42 amino acid propeptide sequence, and a 190 amino acid mature protein with a theoretical molecular mass of 19904.01 and a pI of 9.13. The putative mature protein of $P.$ $xuthus$ attacin showed 48-52% and 24-30% identity in amino acid sequences with that of lepidopteran and dipteran insects, respectively. Semiquantitive RT-PCR results revealed that the transcript of $P.$ $xuthus$ attacin gene was up-regulated at significant levels after injection with bacterial lipopolysaccharide (LPS). We sub-cloned cDNA fragment encoding mature $P.$ $xuthus$ attacin into the expression vector, highly expressed in $E.$ $coli$ BL21 cells, and its antibacterial activity was analyzed. Recombinant $P.$ $xuthus$ attacin evidenced considerably antibacterial activity against Gram-negative bacteria, $E.$ $coli$ ML 35 and $Klebsiella$ $pneumonia$.

• Journal of Microbiology and Biotechnology
• /
• 제30권9호
• /
• pp.1305-1309
• /
• 2020

Insects possess biological defense systems that can effectively combat the invasion of external microorganisms and viruses, thereby supporting their survival in diverse environments. Antimicrobial peptides (AMPs) represent a fast-acting weapon against invading pathogens, including various bacterial or fungal strains. A 37-residue antimicrobial peptide, papiliocin, derived from the swallowtail butterfly Papilio xuthus larvae, showed significant antimicrobial activities against several human pathogenic bacterial and fungal strains. Jelleines, isolated as novel antibacterial peptides from the Royal Jelly (RJ) of bees, exhibit broad-spectrum protection against microbial infections. In this study, we developed a novel antimicrobial peptide, PAJE (RWKIFKKPFKISIHL-NH₂), which is a hybrid peptide prepared by combining 1-7 amino acid residues (RWKIFKK-NH₂) of papiliocin and 1-8 amino acid residues (PFKISIHL-NH₂) of Jelleine-1 to alter length, charge distribution, net charge, volume, amphipaticity, and improve bacterial membrane interactions. This novel peptide exhibited increased hydrophobicity and net positive charge for binding effectively to the negatively charged membrane. PAJE demonstrated antimicrobial activity against both gram-negative and gram-positive bacteria, with very low toxicity to eukaryotic cells and an inexpensive process of synthesis. Collectively, these findings suggest that this novel peptide possesses great potential as an antimicrobial agent.

• 디자인학연구
• /
• 제19권6호
• /
• pp.9-14
• /
• 2006

민화나 고화에 많이 등장하는 호랑나비나 제비나비형태의 그림은 다수 있지만 한자 문화권이라 그런지 '나비'라는 말은 늦게 나타난다. 나비라는 말은 나불나불 나는 모습에서 유래되었다고 하는데, 고서에 호접(胡蝶:범나비), 황접(黃蝶:노랑나비) 등으로 나타나다가 1481년에 나온 두시언해(杜詩諺解)에서 나비 또는 나뵈로, 1527년에 나온 훈몽자회(訓夢字會)에는 나뵈로, 숙종 시대(1675$\sim$1720)에 나온 시몽언해물명(時夢諺解物名)에는 남이로 나온다. 그 후로는 나?????? 또는 나비로 불러오다가 현재 나비가 표준말이 되어 쓰여 지고 있다. 아직도 지방에 따라서는 나부 또는 나베라고 부르는 곳도 있다. 나비는 연인의 사랑 기쁨 행운 장수 영원 등의 의미와 아름다운 자태에 마음이 끌려 예로부터 시와 그림으로 표현되어 그 아름다움을 감상하거나, 공예품, 장신구등에 응용되어 왔다. 본 연구는 이러한 나비의 형태와 색채를 분석하고 수용성 부직포를 이용한 누비 응용기법을 적용하여 나비의 형태미, 날개시맥의 섬세한 조형미를 표현하였으며 가볍고 드레이프성이 좋은 기본소재를 사용함으로서 나비의 날개 짓이 주는 율동적 움직임을 용이하게 표현될 수 있도록 하였다. 이에 본 연구는 상기의 표현 기법이 나비 이미지의 미적표현에 적합함과 아울러 디자인 전개에 무한한 잠재력을 가지고 있음을 제시하고자 한다. 연구 결과 나비 날개 윤곽선의 부드러운 곡선은 인체의 선적 조형미에 잘 부합되어 의상과 인체가 조화롭게 결합될 수 있다는 것을 알 수 있었다. 또한 누비 응용 기법이 가진 특성들이 소재 표현기법으로 다양화 될 수 있음을 깨달았으며 누비 기법의 응용을 통한 혼합기법이 의상에 적용됨으로서 오뜨 꾸뛰르(Haute Couture) 적인 고급스러운 작품으로 전개될 수 있음을 알게 되었다.

• 한국자기공명학회논문지
• /
• 제19권2호
• /
• pp.54-60
• /
• 2015

Papiliocin, from the swallowtail butterfly, Papilio xuthus, shows high bacterial cell selectivity against Gram-negative bacteria. Recently, we designed a 22mer analog with N-terminal helix from $Lys^3$ to $Ala^{22}$, PapN. It shows outstanding antimicrobial activity against Gram-negative bacteria with low toxicity against mammalian cells. In this study, we determined the 3-D structure of PapN in 300 mM DPC micelle using NMR spectroscopy and investigated the interactions between PapN and DPC micelles. The results showed that PapN has an amphipathic ${\alpha}$-helical structure from $Lys^3$ to $Lys^{21}$. STD-NMR and DOSY experiment showed that this helix is important in binding to the bacterial cell membrane. Furthermore, we tested antibacterial activities of PapN in the presence of salt for therapeutic application. PapN was calcium- and magnesium-resistant in a physiological condition, especially against Gram-negative bacteria, implying that it can be a potent candidate as peptide antibiotics.

• Journal of Microbiology and Biotechnology
• /
• 제20권8호
• /
• pp.1185-1188
• /
• 2010

Previously, papiliocin was isolated from the swallowtail butterfly Papilio xuthus and its antimicrobial activity was suggested. In this study, the antifungal mechanism of papiliocin against Candida albicans was investigated. Confocal laser scanning microscopy (CLSM) and 1,6-diphenyl-1,3,5-hexatriene (DPH) fluorescence analysis indicated that papiliocin disturbed the fungal plasma membrane. Moreover, the assessment of the release of FITC-dextran (FD) from liposomes further demonstrated that the antifungal mechanism of papiliocin could have originated from the pore-forming action and that the radius of the pores was presumed to be anywhere from 2.3 to 3.3 nm.