• Journal of Periodontal and Implant Science
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• v.34 no.1
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• pp.71-81
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• 2004

Peri-implantitis could be the result of biomechanical and occlusal overload as well as microbiologic invasion. The dental implant may be more susceptible to dental plaque than the natural tooth, as the predictability of a stable soft tissue attachment complex has not yet been confirmed. With the development of peri-implantitis, the implant surface would be exposed to the oral environment and becomes coated with bacteria. The objective of therapy for this condition is to regain integration of the implant with bone. Since fibroblast adherence to surfaces is impeded by endotoxin, it would seem that decontamination would be desirable to obtain maximum osseointegration. The purpose of this study was to determine whether various chemotherapeutic and mechanical treatments(distilled water, air-powder abrasive, hypersaturated citric acid, tetracycline) can detoxify contaminated titanium implant surface by means of kinetic LAL test. Experimental rough surface titanium disks were fabricated. All of them were divided into two groups(A.a group and P.g group) and each contaminated by A. actinomycetemcomitans and P. gingivalis suspension. Contaminated disks were treated with distilled water, air-powder abrasive, citric acid and tetracycline, and then all disks were placed into LPS-free water for elution. The results were as follows : 1. In A.a group, LPS elute level of all test groups were significantly lower than control group(p<0.05). 2. In A.a group, LPS elute level of test 2, test 3 and test4 groups were significantly lower than that of control group(p<0.05). But, among the test 2, test 3, test4 groups, the significant differences were not detected. 3. In P.g group, LPS elute level of test 2, test 3 and test 4 groups were lower than that of control group(p<0.05). But, among the test groups, the significant differences were not detected. From the result of this study, it would be concluded that air-powder abrasive, hypersaturated citric acid and tetracycline treatments may be effective at reducing endotoxin level on rough titanium implant surfaces, and can be clinically used. But the treatments in peri-implantitis differentially impact osseointegration making one method clinically superior. To gain this knowledges, further molecular biologic and histopathologic studies should be developed.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 1993.04a
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• pp.86-92
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• 1993

자동차의 현가장치에 대한 능동제어연구는 국내외적으로 활발히 진행되어 왔다. 수동식현가장치는 단순히 스프링과 감쇠기로 차체의 진동을 수동 제어 하므로 성능 향상에 한계가 존재하게 된다. 수동식 현가장치가 강성계수와 감쇠계수를 조절함으로써 차체로 들어오는 진동을 억제하는 반면, 능동식제 어는 보통 유압을 이용하여 효율적으로 차체에 들어오는 진동을 억제시키게 된다. 일반적으로 자동차가 능동현가장치 설계시 요구되는 사항은 탑승자의 승차감, 조종성, 현가장치의 공간확보 문제, 경제성(제어력), 실제적으로 자동 차에 적용할 수 있는 능동제어기법인가 하는 문제이다. 자동차 능동식 현가 장치는 보통 1/4 car (2자유도계), Full-car 모델 (7자유도계) 등으로 모델링 을 하여 능동제어기를 설계한다. 1/4 car 모델의 특징은 해석이 비교적 단순 하고 현가장치의 동적거동을 이해하는데 유용하고 실험을 하거나 실제 자동 차에 적용하기 쉬운 반면에 Full-car 모델에 비해 제어력의 효율이 떨어진다 는 단점이 있다. 그 이유는 1/4 car 모델은 차체의 동역학적 특성을 고려하 여 설계하지 않았기 때문에 4개의 독립현가차축에서는 오직 그 현가축방향 으로 발생하는 수직방향의 진동만을 제어하기 때문이다. 따라서 동역학적 역 성에 기인하는 운동을 제어하는 비효율적인 제어력이 공급된다는 단점을 갖 는다. 이에 비해 full-car 모델은 주행모드(수직, 롤링, 피칭운동)간의 연성을 고려하여 제어기를 설계할 수 있기 때문에 1/4 car 모델에 비해 제어력의 효 율이 높다는 장점이 있는 반면에 모델이 수학적으로 복잡하므로 제어력을 구하는데 계산량이 많고, 실제 자동차에 적용하기에 복잡하다는 단점을 갖고 있다. 따라서 본 논문에서는 쉽게 실험할 수 있고, 실용화할 수 있는 1/4 car 모델에 대하여 능동제어기를 설계하여 실제자동차에 능동제어기를 적용할 때 참고가 될 수 있도록 하였다. 자동차는 저주파영역의 밴드통과필터 역할 을 하므로 저주파에서의 성능, 특히 탑승자가 민감하게 느끼는 0.5Hz - 10Hz 부근의 주파수성능은 승차감, 조종성에 상당히 중요하다. 이에 본 논문 에서는 0.5Hz - 10Hz 부근의 승차감, 조종성의 향상에 초점을 두고 차체의 속도를 출력변수로 한 LQG/LTR 제어기를 설계하였다. LQG/LTR 설계기법 은 안정도-강인성이 좋은 체계적인 설계기법으로서 전 상태를 측정할 필요 가 없으므로 실제 적용시 효과적이다. 또한 자동차의 제원의 변화에 대한 고 유치의 민감도해석과 새로운 개념으로 안정도-강인성(Robustness)해석을 하 여 수동시스템과 능동시스템의 강인성을 비교하였다.

• Korean Journal of Soil Science and Fertilizer
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• v.25 no.1
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• pp.77-88
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• 1992

Effective strains of cowpea bradyrhizobia JB7 $nal^rspe^r$ and CB756 $str^rrif^r$, antibiotic-resistant variants of JB7 and CB756, respectively, were used to examine changes of rhizosphere populations and nodule occupancy. Populations of each strain increased gradually in the rhizosphere, reaching a maximum of about $10^8$ cells per root system. Nodule number increased as the density of inoculum increased from $10^2$ cells to $10^8$ cells per seed. Inoculation with liquid suspension resulted in the formation of more nodules than the peat slurry or granule inoculation. When JB7 $nal^rspe^r$ and CB756 $str^rrif^r$ were introduced in equal numbers in inoculum mixtures the former consistantly occupied the majority of nodules with all three groundnut cultivars used. There was no difference in yield between nitrogen treatments and inocultation treatments.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.12
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• pp.797-803
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• 2019

This study was carried out to reduce the lateral vibration of high-speed electric multiple units. In the study, the high-speed electric multiple unit prototype (HEMU-430X) has a high lateral vibration at low equivalent conicity regardless of the wheel profiles (XP55, GV40, S1002). As wheel wear progresses and the equivalent conicity increases, the lateral vibration tends to decrease. The reason is that a combination of the suspension characteristics causes the body and bogie to resonate at a frequency of 1.4 Hz when the equivalent conicity is low, resulting in body hunting. An investigation of the lateral vibration of overseas high-speed trains showed that a decrease in the hydraulic stiffness of the yaw damper could improve the vibration. The series stiffness of the yaw damper is a combination of the hydraulic stiffness and elastic joint. In this study, an attempt was made to improve the lateral vibration by lowering the stiffness of the elastic joint. The series stiffness of the adjusted yaw damper was approximately 60% compared to the original one. The on track test results showed improvement in the lateral vibration for both running directions. The vibration reduction method of this study can be used for EMU-250 and EMU-320 in future commercial operations.

• 한국유가공학회:학술대회논문집
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• 2005.06a
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• pp.37-61
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• 2005

Microcapsules consisting of natural, biodegradable polymers for controlled and/or sustained core release applications are needed. Physicochemical properties of whey proteins suggest that they may be suitable wall materials in developing such microcapsules. The objectives of the research were to develop water-insoluble, whey protein-based microcapsules containing a model water-soluble drug using a chemical cross-linking agent, glutaraldehyde, and to investigate core release from these capsules at simulated physiological conditions. A model water soluble drug, theophylline, was suspended in whey protein isolate (WPI) solution. The suspension was dispersed in a mixture of dichloromethane and hexane containing 1% biomedical polyurethane. Protein matrices were cross-linked with 7.5-30 ml of glutaraldehyde-saturated toluene (GAST) for 1-3 hr. Microcapsules were harvested, washed, dried and analyzed for core retention, microstructure, and core release in enzyme-free simulated gastric fluid (SGF) and simulated intestinal fluid (SIF) at 37$^{\circ}C$, A method consisting of double emulsification and heat gelation was also developed to prepare water-insoluble, whey protein-based microcapsules containing anhydrous milkfat (AMF) as a model apolar core. AMF was emulsified into WPI solution (15-30%, pH 4.5-7.2) at a proportion of 25-50% (w/w, on dry basis). The oil-in-water emulsion was then added and dispersed into corn oil (50 $^{\circ}C$)to form an O/W/O double emulsion and then heated at 85$^{\circ}C$ for 20 min for gelation of whey protein wall matrix. Effects of emulsion composition and pH on core retention, microstructure, and water-solubility of microcapsules were determined. Overall results suggest that whey proteins can be used in developing microcapsules for controlled and sustained core release applications.

• Korean Journal of Environmental Agriculture
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• v.35 no.1
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• pp.72-78
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• 2016

BACKGROUND: Entomopathogenic fungi have been studied to develop for biological control agents as an alternative to chemical control agents in insect pest management. This investigated to determine the optimal culture conditions in ceramic balls for maximal sporulation of entomopathogenic fungi Beauveria bassiana M130 by use rice bran extract.METHODS AND RESULTS: METHODS AND RESULTS: A culture of entomopathogenic fungi for 12day on rice bran extract(1:8, w/v) incubated in ceramic matrix at 28℃. Natural zeolite ceramic ball was high production of 4.2×108 conidial/mL. The culture condition optimized initial pH, temperature, rice bran extract concentration, adhesives substance and concentration of NaCl, respectively. The high production of spore optimal conditions were temperature 28℃, initial pH 3, rice bran extract 3 mL, starch 33 g, 5 % NaCl and sopre suspension 7 mL, respectively.CONCLUSION: This study was carried out for the mass production of entomopathogenic fungi conidia recover rate 65% in matrix of natural zeolite ceramic ball, and to develop ingredient-used formulation of Beauveria bassiana M130 conidia for biological control agents.

• Journal of Life Science
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• v.24 no.12
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• pp.1301-1307
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• 2014

Adipose-derived stem cells (ADSCs) are considered promising tools for tissue regeneration. However, ADSCs have very poor proliferation capacity. Therefore, fetal bovine serum (FBS) is generally added to the culture media of ADSCs. As FBS contains many uncharacterized components that may affect cellular functions, methods for serum-free cultures of ADSCs have been widely investigated. In this study, to develop an efficient method for a serum-free culture of ADSC-T, we used an ADSC line established by introducing the simian virus 40 (SV40) T gene into primary ADSCs. We then investigated the effect of amino acids, vitamins, and other components on the growth of ADSC-T. When the ADSC-T cells were plated with DMEM/F12 serum-free medium, the cells did not proliferate, and the mixture of amino acids, vitamins, and B27 supplement did not increase the growth of the cells. However, when the ADSC-T cells were provided with serum-free DMEM/F12 after they had been cultured with serum-supplemented DMEM for 24 h, the cells proliferated, and the vitamins and B27 supplement increased the cell growth. Stem-Pro serum-free medium also appeared to be useful as a suspension culture for the ADSC-T cells. The ADSC-T cells secreted large amounts of proteins of around 70 kDa. Insulin-like growth factor (IGF) and fibroblast growth factor basic (FGF basic) were secreted by ADSC-T in larger amounts in the serum-free culture than in the serum-supplemented culture.

• Korean Journal of Food Science and Technology
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• v.31 no.3
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• pp.788-793
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• 1999

In this study, it was investigated that the isolation and identification of spoilage bacteria from contaminated aseptic packaged cooked rice, the potential for application of acidic electrolyzed saline solution (AESS) as water-for-cooked rice and the microbiological safety of AESS-based cooked rice. Five strains of Bacillus subtilis and a B. cereus strain among the total six isolates were partially identified by biochemical method and by Microbial Identification System (MIS). The bactericidal effect of AESS was similar as 0.1% NaOCl and 70% ethanol solution, or better than that. All of the test microorganisms except Bacillus spp. that were exposed to AESS for five seconds were destructed. The effect of AESS against Bacillus spp. was much better than that of the two solutions and all of them were destructed or inhibited on exposure for five minutes. The pH value of cooked rice prepared using AESS was in the range of 3.6 to 4.3 and was not almost changed through the storage period. Various concentrations of cell suspension of Bacillus isolates were inoculated to cooked rice, which were prepared with tap water and AESS, and stored at $37^{\circ}C$ for two weeks. The result was shown that the bacteria in tap water-based cooked rice appeared normal cell growth, while they were completely repressed in AESS-based cooked rice.

• The Korean Journal of Food And Nutrition
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• v.14 no.2
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• pp.161-166
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• 2001

The optimum condition for production of yeast extract by autolysis and enzymatic method were studied with waste brewers\` yeast. The major components of brewer\`s yeast in proximate compositions were carbohydrate(46.0%) and protein(41.7%). There was a little appreciable difference in the promaximate composition of waste brewers\` and bakery yeast. The major minerals were identified as K, P, Mg and Ca, and their contents were about 1,659.7%, 1,197.4mg%, 210.4mg% and 105.6mg%, respectively. The major vitamins were identified as vitamin C and vitamin B group, and their contents were about 7.9IU/100g and 4.2IU/100g, respectively. The optimum yeast concentration in the reaction suspension was 10% (w/v) waste brewer\`s yeast slurry. The protein content in brewer\`s yeast extract was increased with addition of NaCl as plasmolyzers. However, the plasmolyzer effect of ethanol a little showed as the concentration increasd from 3% ∼7%. The 5\`-nucleotides(5\`-GMP and 5\`-IMP) content in brewer\`s yeast extract was increased with addition of glucanase. The total content of 5\`-nucleotides (5\`-GMP and 5\`-IMP) increased by 160% than those by the autolysis of waste brewer\`s and bakery yeast.

• Journal of Plant Biotechnology
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• v.30 no.4
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• pp.381-385
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• 2003

An efficient method of plant regeneration from Acanthopanax chiisanensis somatic embryos was developed. Cotyledonary somatic embryos were obtained in liquid Murashige and Skoog (MS) medium from embryogenic cell suspension cultures. They were desiccated for 0 to 72 hr and then cultured on MS medium containing NAA, BA, GA$_3$, (0-0.5mg/L). The highest multiple shoots formation (100％) was obtained from 72 hr desiccated somatic embryos on ifs medium with 0.5mg/L NAA＋0.5mg/L BA or 0.5 mg/L NAA＋0.5mg/L BA＋0.5mg/L GA$_3$ after 6 weeks culture. Plant conversion from multiple shoots was not high. The highest plant conversion from multiple shoots was obtained on 1/3MS medium with 1.0mg/L GA$_3$. Converted plantlets were transferred to ex vitro condition and the highest survival rate (70％) of the plantlets was obtained on plastic pots containing vermiculite and sand. These results indicate that micropropagation procedure can be applied for an efficient mass propagation of Acanthopanax chiisanensis.