Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Plant Production from Desiccated Somatic Embryos of Acanthopanax chiisanensis

지리오가피 (Acanthopanax chiisanensis) 체세포배의 건조처리를 통한 식물체 증식

  • Published : 2003.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

An efficient method of plant regeneration from Acanthopanax chiisanensis somatic embryos was developed. Cotyledonary somatic embryos were obtained in liquid Murashige and Skoog (MS) medium from embryogenic cell suspension cultures. They were desiccated for 0 to 72 hr and then cultured on MS medium containing NAA, BA, GA$_3$, (0-0.5mg/L). The highest multiple shoots formation (100％) was obtained from 72 hr desiccated somatic embryos on ifs medium with 0.5mg/L NAA＋0.5mg/L BA or 0.5 mg/L NAA＋0.5mg/L BA＋0.5mg/L GA$_3$ after 6 weeks culture. Plant conversion from multiple shoots was not high. The highest plant conversion from multiple shoots was obtained on 1/3MS medium with 1.0mg/L GA$_3$. Converted plantlets were transferred to ex vitro condition and the highest survival rate (70％) of the plantlets was obtained on plastic pots containing vermiculite and sand. These results indicate that micropropagation procedure can be applied for an efficient mass propagation of Acanthopanax chiisanensis.

지리오가피의 체세포배로부터 식물체재생을 위한 효율적인 방법을 개발하였다. 체세포배의 형태형성에 미치는 식물생장 조절물질의 영향을 조사하고자, 접합자 배 유래의 배발생 세포를 MS배지에 현탁배양하고, 이로부터 얻어진 자엽시기의 배를 배양접시에서 건조처리 (0∼72 h)한 후, NAA, BA, GA$_3$(0∼0.5mg/L)가 첨가된 MS배지에 배양하였다. 그 결과, 체세포배를 72시간 동안 배양접시에서 건조처리 후에 0.5mg/L NAA, 0.5mg/L BA 또는 0.5mg/L NAA, 0.5mg/L BA 0.5mg/L GA$_3$가 첨가된 MS배지에 6주간 배양하였을 때, multiple shoot가 100%로서 가장 높게 유도됨을 관찰 할 수 있었다. 가장 높은 식물체의 재생율 (29.1%)은 체세포배를 72시간 건조처리 후에,0.1mg/L NAA, 0.1mg/L BA그리고 0.1mg/L GA$_3$가 첨가된 MS배지에 6주간 이식한 후, 이를 다시 1.0mg/L GA$_3$가 첨가된 1/3MS배지에 6주간 배양하였을 때 이루어졌다. 유식물체를 vermiculite와 sand가 1:1로 첨가된 plastic pot 이식하였을 때 생존율은 약 70%로서 나타났다 이상의 결과는 지리오가피의 계량생산에 활용될 수 있을 것으로 기대된다.

Keywords

References

  1. Choi YE, Kim JW, Soh WY (1997) Somatic embryogenesis and plant regeneration from suspension cultures of Acanthopanax koreanum Nakai. Plant Cell Rep 17: 84-88 https://doi.org/10.1007/s002990050357
  2. Choi YE, Yang DC, Park JC, Soh WY, Choi KT (1998) Regenerative ability of somatic single and multiple embryos arising directly from cotyledons of Panax ginseng. Plant Cell Rep 17: 544-551 https://doi.org/10.1007/s002990050439
  3. Choi YE, Jeong JH (2002) Dormancy induction of somatic embryos of Siberian ginsengby high sucrose concentrations enhances the conservation of hydrated artificial seeds and dehydration resistance. Plant Cell Rep 20: 1112-1116 https://doi.org/10.1007/s00299-002-0455-y
  4. Choi YE, Ko SK,Lee KS, Yoon ES (2002a) Production of plantlets of Eleutherococcus sessiliflorus via somatic embryogenesis and successful transfer to soil. Plant Cell Tiss Org Cult 69: 201-204 https://doi.org/10.1023/A:1015290718273
  5. Choi YE, Lee KS, Kim SY, Han JY, Kim HS, Jung JH, Ko SK (2002b) Mass production of Siberian ginseng plantlets through large-scale tank culture of somatic embryos. Plant Cell Rep 21: 24-28 https://doi.org/10.1007/s00299-002-0470-z
  6. Gui Y, GuoZ, Ke S, Skirvin RH (1991) Somaticembryogenesis and plant regeneration in Acanthopanax senticosus. Plant Cell Rep 9: 514-516
  7. Isoda S, Shoji T (1994) Studies on the cultivation of Eleutherococcus senticosus Maxim. II. On the germination and raising of seedling. Nat Med 48: 75-81
  8. Lee EK, Cho DY, Soh WY (1997) Effect of humidity on somatic embryogenesis in cotyledon explantculture of carrot. J Plant BioI 40: 89-94 https://doi.org/10.1007/BF03030239
  9. Lee EK, Cho DY, Soh WY (2001) Enhanced production and germination of somatic embryos by temporary starvation in tissue cultures of Dacues carota. Plant Cell Rep 20: 408-415 https://doi.org/10.1007/s002990100338
  10. Lee KS, Choi YE, Sim OK, Joo SA, Shin JS, Jeong JH, Kim YS, Kim EY (2002a) Effects of $GA_3$ and charcoal on plant pregeneration from somatic embryos of Acanthopanax sessiliflrorus. Kor J Plant Biothechnology 29: 253-257
  11. Lee KS, Lee JC, Soh WY (2002b) High frequency plant regeneration from Aralia cordata somatic embryos. Plant Cell Tiss Org Cult 68: 241-246 https://doi.org/10.1023/A:1013989707725
  12. Mathews H, Schopk C, Carcamo R, Chavarriaga P, Fauquet C, Beachy RN (1993) Improvement of somatic embryogenesis and plant recovery in cassava. Plant Cell Rep 20: 328-333
  13. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15: 473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  14. Rajasekaran K, Mullins MG (1979) Embryos and plantlets from cultured anthers of hybrid grapevines. J Exp Bot 30: 399-407 https://doi.org/10.1093/jxb/30.3.399
  15. Schenk RU, Hildebrandt AC (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can J Bot 50: 199-204 https://doi.org/10.1139/b72-026
  16. Soh WY (1993) Developmental and structural diversity of regenerated plants in cell and tissue cultures. In: Kim SG (ed), Molecular Approach to Plant Cell Differentiation. Proc 7th Symp Plant Biotechnol. Botanjcal Society of Korea, Seoul, pp 1-35
  17. Yehoshua S, Rhodes D, Janick J (1992) Changes in amino acid composition associated with tolerance to partial desiccation of celery somaticembryos. J Am Soc Hort Sci 117: 337-341
  18. Zimmerman JL, Apuya N, Darwish KO, Carroll C (1989) Novel regulation of heat shock genes during carrot somatic embryo development. Plant Cell 12: 1137-1146

Cited by

  1. Micropropagation via somatic embryogenesis of rare and endangered species,Acanthopanax seoulensesNakai vol.10, pp.4, 2014, https://doi.org/10.1080/21580103.2014.913536