• 한국임상수의학회지
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• 제2권1호
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• pp.133-141
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• 1985

In order to establish a method predicting susceptible cows to the parturient syndrome, various serum chemical parameters (calcium, phosphorus, Ca/P, magnesium, cholesterol, total protein, albumin, globulin, A/G, total lipid, non-esterified fatty acid(NEFA) and aspartate aminotransferase(AST)) were measured during late pregnancy and their relationships with periparturient diseases were investigated during puerpural period. The results obtained were as follows : 1. The factors affecting the prediction of susceptible cows to parturient syndrome were calcium, magnesium, total protein, globulin, A/G ratio and total lipid at 30 day antepartum and the diagnosability was 70.7%. 2. In the experimental cows producing more than 21kg of milk per day, the factors affecting the prediction of susceptible cows to parturient syndrome were calcium, NEFA and A/G ratio at 30 day antepartum and the diagnosability was 66.7%. 3. In the experimental cows calved more than 3 times, the factors affecting the perdiction of susceptible cows to parturient syndrome were calcium, total protein, albumin and NEFA at 30 day antepartum and the diagnosability was 83.3%.

• The Plant Pathology Journal
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• 제31권2호
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• pp.132-139
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• 2015

Bt-cotton germplasm, consisting of 75 genotypes was evaluated against cotton leaf curl disease (CLCuD) under high inoculum pressure in the field and using graft inoculation in glasshouse by visual symptom scoring assessments. None of the tested genotype was found disease free under both evaluation tests. Under field conditions in 2011, 3 genotypes were found resistant, 4 moderately resistant, 3 tolerant, 2 moderately susceptible and one susceptible; in 2012, 3 genotypes were tolerant, 7 moderately susceptible, 5 susceptible and 38 highly susceptible; in 2013, one was moderately susceptible and 51 were highly susceptible with varying degree of percent disease index (PDI) and severity index (SI). However, through graft evaluation in glasshouse, none of the graft inoculated plant was symptomless. All tested genotypes showed disease symptoms with SI values ranging between 5.0 and 6.0, and latent period between 12 and 14 days. Of the 75 genotypes evaluated using graft inoculation, 11 were found susceptible with SI values of 5.0 to 5.4 while remaining 64 were highly susceptible with SI values of 5.5 to 6.0. Inoculated plants of all tested genotypes exhibited severe disease symptoms within 10 days after the appearance of initial symptoms. No reduction in SI value was observed until the end of the experiment i.e., 90 days after grafting. Information generated under the present study clearly demonstrates that no sources of resistance to CLCuD are available among the tested Bt-cotton genotypes. So, a breeding programme is needed to introgress the CLCuD-resistance from other resistant sources to agronomically suitable Bt-cotton genotypes.

• The Plant Pathology Journal
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• 제23권2호
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• pp.103-105
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• 2007

Mechanical transmission of barley seedlings with barley yellow mosaic virus (BaYMV) is generally inefficient and is the major constraint for testing cultivar resistance to the virus. To explore mechanical transmission, BaYMV-infected barley plants were grown at different conditions and used as inoculum sources to seedlings of susceptible barley cultivar Baegdong. Extracts prepared from BaYMV-infected Baegdong plants at 47, 53, 74, and 90 days after symptom appearance (DASA) and grown at 10 and $12^{\circ}C$ gave 10, 30, 68 and 76% infection, respectively on inoculated susceptible barley cv. Baegdong seedlings. While Jinyangbori, another susceptible cultivar obtained 95% infection rate inoculated with extracts from 90 DASA disease source and grown at $10/12^{\circ}C$. However, low infection rates were obtained when the virus sources were grown in a greenhouse at $15-18^{\circ}C$. Our results indicate that longer incubation period and lower temperature are required for virus accumulation and stability.

• 한국작물학회지
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• 제44권4호
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• pp.373-381
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• 1999

Six soybean cultivars having different SDS susceptibility were planted with sorghum seedinoculum infested with F. solani isolate 171 in the greenhouse. First leaf symptoms appeared on unifoliar leaves at 9 days after inoculation and all cultivars showed the typical leaf symptoms at 13 days after inoculation, when trifoliar leaves emerged. Leaf symptoms development in susceptible cultivars was faster than in resistant cultivars. Leaf symptom severities during the period of 25 to 29 days after inoculation showed a significant difference between cultivars which had SDS resistance and sus ceptibility. In this period, area under the diseaseprogress curve (AUDPC) of Hartz 6686 was the highest and that of PI 520733 was the lowest. SDS caused serious damage to the growth of soybean in all cultivars. Average reductions of growth rate of root fresh weight and dry weight were greater than those of plant tops. Duyu-kong showed less severe leaf symptoms than that of SDS suscetible cultivars; however, average growth rate of plants top and roots of this cultivar was less but not significantly different than those of SDS susceptible cultivars. In all cultivars, as severity of leaf symptoms increased, plant top weight decreased. Root rot symptoms were observed in all cultivars before leaf symptoms appeared. Average proportions of tap root reddish-brown discoloration of all cultivars was up to 75 % at 15 days after inoculati on; however there was no significant differenc between cultivars at each rating date. Appearances of leaf symptoms on leaves varied in each cultivar. SDS resistant cultivars had a significantly higher level of crinkling than susceptible cultivars and SDS susceptible cultivars had a significantly higher level of necrosis than resistant cultivars. Further study will be needed to identify the relationships between the physiological growth rate and SDS severities in soybeans.

• 한국응용곤충학회지
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• 제40권4호
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• pp.321-326
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• 2001

Wolbachia 감염이 디코폴 감수성계통 점박이응애와 저항성계통 점박이응애 적합도에 미치는 영향(발육, 수명, 산란수, 산란기간)을 미니장미를 기주로 조사하였다. ftsZ PCR결과 감수성 계통만이 Wolbachia에 감염된 것으로 조사되었다. 감수성계통은 저항성계통에 비해 높은 산란율과 빠른 발육률이 관찰되었으며, 성충수명과 산란기간은 감수성계통에서 길었다. 이는 적합도가 디코폴저항성 수준에 영향을 받고 있음을 보여주고 있다. 동일한 조사를 상호교배 자손에서 조사한 결과, Wolbachia에 감염된 수컷과 Wolbachia 비감염 암컷을 교배한 조합에서 높은 난사망율과 수컷편중 성비가 관찰되어 전형적인 Wolbachia에 의해 유도되는 세포질 불화합성을 보여주고 있다. 또한 Wolbachia에 감염된 암컷과 Wolbachia 비감염 수컷조합의 내적자연증가율값($0.09\pm$0.01)은 다른 조합($0.20\pm$0.01)에 비해 유의하게 낮았다. 본 실험의 연구결과는 점박이응애 적합도의 발달과 진화가 저항성 수준과 Wolbachia 감염에 밀접하게 연관되어 있음을 보여주고 있다.

• 한국응용곤충학회지
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• 제14권3호
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• pp.133-136
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• 1975

저항성 및 감수성 콩 품종이 콩씨스트선충(Heterodeta glycines)의 발육에 미치는 영향을 알기 위하여 시험하였다. 콩 품종별로 선충의 이기유총$(L_2)$에 대한 유인성의 차이는 없었다. 다만, 저항성품종에서는 선충의 발육상태가 좋지않아 10주동안에 2세대만 발생하였으며 그 밀도가 매우 낮은 반면에 감수성품종에서는 선충의 발육이 정상이어서 같은 기간에 3세대 발생하는 것을 볼 수 있으며 그 밀도는 매우 높았다.

• 한국응용곤충학회지
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• 제12권4호
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• pp.139-142
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• 1973

흰등멸구(Sogatella furcifera Horvath)에 대한 IRRl 선발 및 국내장려품종의 저항성정도를 검정하고 나아가 몇 가지 저항성기작을 검토코저 시험을 실시하여 아래와 같은 결과를 얻었다. 1) IRRI 선발품종 13개 중 저항성반응을 보인 품종은 Columbia, Muthumanikam, Pankhari-203등 3품종이었고 그밖에 H-105 외 9개 품종은 감수성반응을 나타내었다. 2) 국 내품종 20개에 대한 검정결과는 모두 감수성반응을 나타내었다. 3) 저항성반응을 나타낸 Muthumanikam과 Pankhari-203에서 흰등멸구의 약충기간은 각각 14.7일과 16.0일 이었고, 감수성반응을 보인 품종들에서는 10.1-10.6일이었으며 중도감수성품종 Mudgo는 11.6일 이었다. 4) 약충의 사충율은 저항성품종에서 높고 감수성품종에서 낮았으며 우화율은 저항성품종에서 낮고 감수성품종에서 높았다. 5) 우화율은 저항성품종 Muthumanikam에서는 $20\%$ 이었으나 Pankhari-203에서는 한 마리의 우화도 볼 수 없었다. 중도감수성품종 Mudgo에서는 $60\%$의 우화율을 보였고 감수성품종 T(N)-1과 Suweon 214는 $73\%$, Suweon 213-1은 $87\%$, Paltal은$93\%$, Jaekun과 Suweon 82는 $100\%)$의 우화율을 보였다. 6) 이상의 결과로 보아 흰등멸구에 대하여 저항성을 나타내는 벼 품종은 높은 Antibiosis 현상이 있음을 알 수 있었다.

• The Plant Pathology Journal
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• 제19권5호
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• pp.253-256
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• 2003

Citrus canker disease is caused by bacteria Xanthomonas axonopodis .pv. Citri. Shiranuhi cultivar, a hybrid of Kiyomi tangor and Nakano No.3 ponkan was evaluated for resistance to citrus canker based on initiation of disease, percent area of lesion infected and growth rate of bacteria in the leaf under growth chamber condition. Significant differences between susceptible plant and resistant plants were observed in these assays. Resistant plants showed delayed disease symptoms compared to the susceptible plants after spray inoculation of the pathogen. The resistant verities, satsuma, yuzu, and Shiranuhi showed symptoms after six days where as susceptible, mexican lime showed the symptoms just after three days of inoculation. 18 days after inoculation, percent area of lesions developed on leaf and disease severity differed significantly in susceptible and resistant plants, and were ranked as follows: mexican lime > early satsuma =Shiranuhi =yuzu (P <, 0.01). However, 30 days after inoculation, percent area of lesion was further differentiated into resistant and highly resistant plants. That was ranked as follows: sweet orange> early satsuma =Shiranuhi =Kiyomi > yuzu (P < 0.01). These results indicate that host reaction to the bacterial was more distinct when the disease developed for a longer period. Growth rates of a citrus canker bacterium during 16 40 h also were distinct after infiltration into leaves of susceptible and resistant plants, and were ranked as follows: sweet orange> early satsuma =Shiranuhi =Kiyomi =yuzu (P < 0.01). Based on these results, we concluded that Shiranuhi is resistant to citrus canker as compared to Kiyomi, early satsuma, and yuzu.

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2008년도 제49회 학술심포지움 및 국제학술대회
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• pp.70.2-70.2
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• 2008

• 한국동물위생학회지
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• 제23권1호
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• pp.77-91
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• 2000

This study was conducted to investigate the antibiotic resistance and plasmid profiles of 58 salmonella spp isolated from mesenteric lymphnodes of slaughter pigs in Kyoungbuk province during the period from September 1997 to June 1998. The results obtained are as follow that all isolates were susceptible to amikacin, gentamicin, ciprofloxacin and enrofloxacin, and the majority of isolates were highly susceptible to norfloxacin, colistin, nalidixic acid and apramycin while they were moderately susceptible to kanamycin, cephalothin, chloramphenicol, ampicillin, trimethoprim and penicillin. The majority of isolates were over 90% resistant rates to lincomycin, sulfadimethoxine, vancomycin, methicillin and erythromycin. The plasmid profiles of 58 salmonella spp are developed 1 to 4 fractions, 0.9 to 29.5 Kb molecular range sizes and U strains (45.5%) were showed plasmid profiles by agarose gel electrophoresis. 5 derby harbored 29.5 Kb and 7 Kb, and S schwarzengrund had 14 Kb and 0.9 Kb harboring sizes. Four of 10 S agona and 2 of 4 S typhimurium were harbored 3.1 Kb and n.5 Kb, respectively. Thirty-five untypable strains are developed variable size fractions its showed small size plasmid profile less than 6 Kb and 22 (62.8%) of them had no detectable plasmids.