• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.3
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• pp.259-268
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• 2006

Miniature inverted transposable elements (MITEs) are abundant genomic components in plant including rice. MITE-transposon display (MITE-TD) is an Amplified Fragment Length Polymorphism (AFLP)-related technique based on MITE sequence. In this study, we used the MITE-AFLP for the analysis of diversity and relation-ship of the 114 japonica accessions. Of the several MITEs, the mPing family was applied to detect polymorphisms based on PCR amplification. The BfaI adaptor primer and the specific primer derived from mPing terminal inverted repeat (TIR) region were used to PCR amplification of 114 accessions. Nine primer pairs produced a total of 160 polymorphic bands. PIC values of the polymorphic bands generated by nine primer pairs ranged from 0.269 (BfaI + ACT) to 0.426 (BfaI + T). Each accession revealed a distinct fingerprint with two primer combinations, BfaI + G and BfaI + C. Cluster analysis using marker-based genetic similarity classified 114 accessions into five groups. MITE-AFLP markers were genetically mapped using a population of 80 BILs (BC1F7) derived from a cross between the rice accessions, Milyang 23 and Hapcheonaengmi 3. Eight of the markers produced with the primer pair BfaI + 0 were mapped on chromosomes 1, 2, 4, 5, 7, and 9. Considering that one MITE-AFLP marker on chromosome 7 was tightly linked to the Rc gene, the MITE-AFLP markers will be useful for gene tagging and molecular cloning.

• Journal of The Korean Society of Agricultural Engineers
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• v.60 no.6
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• pp.43-54
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• 2018

The accurate estimation of reference crop evapotranspiration ($ET_o$) is essential in irrigation water management to assess the time-dependent status of crop water use and irrigation scheduling. The importance of $ET_o$ has resulted in many direct and indirect methods to approximate its value and include pan evaporation, meteorological-based estimations, lysimetry, soil moisture depletion, and soil water balance equations. Artificial neural networks (ANNs) have been intensively implemented for process-based hydrologic modeling due to their superior performance using nonlinear modeling, pattern recognition, and classification. This study adapted two well-known ANN algorithms, Backpropagation neural network (BPNN) and Generalized regression neural network (GRNN), to evaluate their capability to accurately predict $ET_o$ using daily meteorological data. All data were obtained from two automated weather stations (Chupungryeong and Jangsu) located in the Yeongdong-gun (2002-2017) and Jangsu-gun (1988-2017), respectively. Daily $ET_o$ was calculated using the Penman-Monteith equation as the benchmark method. These calculated values of $ET_o$ and corresponding meteorological data were separated into training, validation and test datasets. The performance of each ANN algorithm was evaluated against $ET_o$ calculated from the benchmark method and multiple linear regression (MLR) model. The overall results showed that the BPNN algorithm performed best followed by the MLR and GRNN in a statistical sense and this could contribute to provide valuable information to farmers, water managers and policy makers for effective agricultural water governance.

• The Journal of the Korea Contents Association
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• v.9 no.5
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• pp.156-165
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• 2009

This research is for dental hygienists to pursue affection and commitment in organization together with improvement of quality of working life by analyzing factors of working life affecting affective commitment in organization of dental hygienists, to find out plans to increase sense of accomplishments of working life and ultimate satisfaction of life, and to suggest direction for human resources management in management of dental clinics. Objects of investigation were dental hygienists working at dental medical institutions in Busan area with questionnaire survey and 197 sheets of questionnaires were used in the study. As the result of the study, high correlation was shown between composition elements of quality of working life and affective commitment per factor and as the result of quality factors of working life affecting affective commitment in organization, work environment, inter-personal relations, and social meaningfulness provided meaningful impacts on affective commitment in organization. It is believed to be important that dental medical institutions need to promote pride and love of companies in the future by providing regular social services or services for local residents so that employees can form smooth inter-personal relationships among employees with improvement of working environment and welfare in personnel management dimension. And for dental hygienists, they shall improve their work performances and enhance vocational values for development of their institutions and them with positive thinking through ceaseless efforts.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.21
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• pp.9341-9346
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• 2014

Acanthopanax trifoliatus (L) Merr (AT) is commonly used as an herbal medicine and edible plant in some areas of China and other Asian countries. AT is thought to have anticancer effects, but potential mechanisms remain unknown. To assess the anticancer properties of AT, we exposed prostate cancer cells to AT extracts and assessed cell proliferation and signaling pathways. An ethanol extract of AT was suspended in water followed by sequential extraction with petroleum ether, ethyl acetate and n-butanol. PC-3 cells were treated with different concentrations of each extract and cell viability was determined by the MTT and trypan blue exclusion assays. The ethyl acetate extract of the ethanol extract had a stronger inhibitory effect on growth and a stronger stimulatory effect on apoptosis than any of the other extracts. Mechanistic studies demonstrated that the ethyl acetate extract suppressed the transcriptional activity of NF-${\kappa}B$, increased the level of caspase-3, and decreased the levels of phospho-Erk1/2 and phospho-Akt. This is the first report on the anticancer activity of AT in cultured human prostate cancer cells. The results suggest that AT can provide a plant-based medicine for the treatment or prevention of prostate cancer.

• Journal of Science and Technology Studies
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• v.18 no.2
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• pp.307-342
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• 2018

This article is to discuss about a debate on controlling and handling rights of diagnostic instruments for the outbreak of Foot-and-Mouth Disease(FMD) in South Korea between 2010 and 2011. The epidemic of FMD caused catastrophic shockwave to the whole Korean society and there was fierce debate what was the best diagnostic tool for the disease and who owned the controlling power. The central government insisted to have the power of controlling the diagnostic tool to maintain the nation-centered disease control while local governments and civil organisations attempted to take over the controlling power of the diagnostic tools. In this article the concept of boundary objects which was suggested by an American STS academic, Susan Leigh Star and her colleagues. The boundary object could be the useful concept for capturing the whole process of constructing and imposing meanings and social orders in the diagnostic tool, called the portable antibody/antigen diagnostic kit. The constructing process of this boundary object must be understood with relation to boundary making activities between different social groups.

• Korean journal of communication and information
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• v.48
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• pp.170-190
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• 2009

The aim of this study is to examine the ideological orientation of Minjujungbo which was the first and the largest newspaper in Busan during the U.S. Military Government in Korea. For this purpose this study analyzed the personal history of the editorial staffs and the coverage of the three historical events. The events analyzed were the supporting statement to trusteeship by Korean Communist Party in Jan. 1946, the money counterfeit by Jungpansa in May, 1946 and the general strike in Sep. 1946. The coverages of Minjujungbo on these three events were compared with those of other newspapers which had shown the orientation of the rightist, the neutral, and the leftist respectively. Most of the editorial staffs had been involved the leftist movement but were not socialist to the core at that time. Most of them were the members of Pusanilbo which was published by Japanese in late colonial period. The coverages of Minjujungbo were analyzed to be similar to those of Seoulsinmun which was evaluated as the neutral. But the coverage of the general strike was analyzed as leftist slightly. In conclusion it can be said that the ideological orientation of Minjujungbo was the left-of-center.

• Journal of Life Science
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• v.19 no.6
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• pp.720-727
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• 2009

Lyophilized powder (BP2FS) of soybeans fermented with Bacillus polyfermenticus KJS-2 (B. polyfermenticus KJS-2) exhibited in vitro antibacterial activities against eight pathogenic bacteria. BP2FS was used as a fodder additive for Oplegnathus fasciatus (0. fasciatus) culture. One group (UFD) of O. fasciatus was fed a commercial fodder, while another group (FD) was fed the same fodder, but including BP2FS ($6{\times}10^{4}$ cfu $g^{-1}$ fodder), two times daily for 120 days. The mean body weight of the FD group (67.29${\pm}$12.62 g) was higher than that of the UFD group (56.56${\pm}$8.21 g) after 120 days. The survival rate of FD was 80% compared to 40% for the UFD group. Cumulative mortalities in the FD and UFD groups were 18.95% and 60.98% respectively. B. polyfermenticus KJS-2 was isolated from the intestines of the FD group and the number of viable colonies was estimated to be $1.04{\times}10^{4}$ cfu $g^{-1}$. Iridovirus and Vibrio vulnificus was detected in the organs of the UFD group but not in the FD group. All of the infected fish showed typical clinical symptoms of hemorrhage in their tail fins. Dissection of the infected internal organs revealed liver congestion and spleen enlargement - typical symptoms caused by iridovirus infection. These results clearly show that BP2FS is highly beneficial in preventing O. fasciatus from iridovirus infection.

• Journal of the Korean Chemical Society
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• v.54 no.4
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• pp.414-418
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• 2010

Ethyl 1-aminotetrazole-5-carboxylate (1) reacted with hydrazine hydrate to give the corresponding aminohydrazide 2. Cyclization of 2 by carbon disulfide yielded 1,3,4-oxadiazole-5-thiol structure 3. Reaction of 3 with either chloroacetone or ethyl chloroacetate furnished S-acyl 1,3,4-oxadiazole derivatives 4 and 5, respectively. Also compound 3 reacted with hydrazine hydrate afforded 4-amino-1,2,4-triazole-5-thiol derivative 6. 6-Methyl-1,3,4-triazolo[3,4-b]-1,3,4-thiadiazole structure 7 was synthesized by reaction of aminothiol 6 with glacial acetic acid. Diazotization of 1 with sodium nitrite in presence of hydrochloric acid yielding the diazonium salt which on treating with hippuric acid, oxazolone derivative 8 was obtained. Furthermore, tetrazolo[5,1-f]-1,2,4-triazine 9 was constructed via cyclization of aminoester 1 with formamide. Compound 9 reacted with carbon disulfide to furnish 8-thione derivative 10 which reacting with chloroacetone, ethyl chloroacetate, and hydrazine hydrate, the corresponding chemical structures 11, 12, and 13 were synthesized. 1,2,4-Triazolo[4,3-d]tetrazolo[5,1-f]-1,2,4-triazines 14 and 15 were resulted by treating of compound 13 with triethyl orthoformate, and glacial acetic acid, respectively. The structures of the newly synthesized products were elucidated according to elemental analyses and spectroscopic evidences. Some of the representative members of the prepared compounds were screened for antimicrobial activity.

• Geophysics and Geophysical Exploration
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• v.10 no.4
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• pp.322-331
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• 2007

In this study, electrical resistivity tomography (ERT) were conducted to find the mineralized zone at the Geumpung mine in Dojeon-ri, Susan-myeon, Jecheon-si, Chungcheongbuk-do. The deviation of the inclined borehole was measured to obtain the exact positions of the electrodes for correcting apparent resistivity values from ERT. Geophysical loggings such as resistivity and natural gamma were conducted to obtain the properties of the material near the borehole. Measurements of the physical properties of the cores, such as porosity, water content, density, susceptibility, resistivity were performed to analyze the correlation between physical properties and resistivity. Grade analysis for core sample was also conducted to identify relationship between grade and resistivity. Rock property analysis shows that the resistivity is more dominated by susceptibility and grade than by porosity and water content in the mineralized zone. The results of ERT are well consistent with geophysical logging data and geologic column. So ERT is powerful method to identify conductive mineralized zone.

• Korean Journal of Ichthyology
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• v.23 no.2
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• pp.135-144
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• 2011

Collections were made monthly using beam trawl from March 2005 to February 2006 in Gujora, and from June 2006 to May 2007 in Geoje Bay to investigate the species composition of Juvenile and Immature Fishes of both coastal areas. Fishes collected in the sandy shore of Gujora were 6 orders, 14 families, 20 species, 224 individuals, and 534.9 g in total. Favonigobius gymnauchen, Trachioncephalus myops, Pseudoblennius percoides, Sagamia geneionema, Repomucenus beniteguri, and Pseudorhombus cinnamoneus were mainly collected. These 6 species accounted for 83.9% of total individuals and 47.1 % of total biomass. On the other hand, fishes collected in Geoje Bay were 4 orders, 8 families, 14 species, 1,406 individuals and 1,899.7 g in total. Acentrogobius pflaumii, Platyce phalusindicus, Chasmichthy dolichognathus, Acanthogobius flavimanus, Gymnogobius urotaenia, and Synechogobius hasta were mainly collected. These 6 species accounted for 97.7% of total individuals and 96% of total biomass. The greatest numbers and biomass of fishes were collected in summer and the lowest in winter. In Geoje Bay, the number of species and individuals reached the peak in September and August, respectively. However, both species and individual numbers were highly fluctuated in Gujora.