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Seroprevalence of Antibodies against Anisakis simplex Larvae among Health-Examined Residents in Three Hospitals of Southern Parts of Korea

  • Kim, Jung;Jo, Jin-Ok;Cho, Seon-Hee;Cho, Min-Kyoung;Yu, Hak-Sun;Cha, Hee-Jae;Ock, Mee-Sun
    • Parasites, Hosts and Diseases
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    • v.49 no.2
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    • pp.139-144
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    • 2011
  • The present study was performed to estimate the seroprevalence of larval Anisakis simplex infection among the residents health-examined in 3 hospitals in southern parts of Korea. A total of 498 serum samples (1 serum per person) were collected in 3 hospitals in Susan Metropolitan city, Masan city, and Geoje city in Gyeongsangnam-do (Province) and were examined by IgE-ELISA and IgE-western blotting with larval A. simplex crude extract and excretory-secretory products (ESP). The prevalence of antibody positivity was 5.0% and 6.6% with ELISA against crude extracts and ESP, respectively. It was also revealed that infection occurred throughout all age groups and higher in females than in males. A specific protein band of 130 kDa was detected from 10 patients with western blot analysis against crude extract and ESP among those who showed positive results by ELISA. Our study showed for the first time the seroprevalence of anisakiasis in Korea. The allergen of 130 kDa can be a candidate for serologic diagnosis of anisakiasis.

Sugar Intakes and Metabokic Syndrome (당 섭취와 대사증후군)

  • Cho, Susan;Kim, Woo-Kyoung
    • Journal of Nutrition and Health
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    • v.40 no.sup
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    • pp.39-49
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    • 2007
  • 본 논문은 미국 의학연구소(US Institute of Medicine, IOM)와 미국 농무성의 식이지침 자문위원회(USDA Advisory Committee on Dietary Guidelines, DG)에서 발표한 권위 있는 종설 들과 미국 영양사협회 학술지를 포함하여 여러 학술지에 발표된 의미 있는 종설 들의 내용을 종합한 것으로 일부의 내용은 그들의 의견을 그대로 반영하였다. 당은 단당류와 이당류를 말하며 당에는 포도당, 과당, 갈락토오즈, 맥아당, 서당과 유당이 포함된다. 당의 식품급원으로는 고과당 옥수수시럽, 사탕수수, 꿀, 사탕수수, 맥아시럽 등이 있다. 당은 미국 식약청에서 일반적으로 안전하다고 인식(generally recognized as safe, GRAS)되고 있다. 당은 에너지를 공급하며, 정신적인 만족감을 부여하는 기능을 가지고 있다. 대부분의 과일과 유제품은 당을 많이 함유하고 있으며, 건강한 식사를 구성하는 식품으로 섭취되고 있다. 당은 또한 조리와 가공과정에서 식품의 맛과 기능적인 면을 향상시키기 위해 첨가되기도 한다. 당은 영양밀도가 높은 식품/음료의 기호성(palatability)를 향상시키므로, 감미료들은 건강한 식사가 되도록 도와줄 수 있다. 그러나 첨가당의 섭취가 증가되면 식사의 영양밀도가 감소하는 가능성에 대한 우려가 있다. 여러 연구에 의하면 첨가당의 섭취가 총 열량 섭취의 25% 이상이 되면 식품의 영양밀도 감소가 나타날 수 있다고 보고하고 있다. 한국에서 추정된 당의 섭취량은 이것보다는 매우 낮은 수준이다. 선진국들에서 당의 섭취가 건강에 부정적인 영향을 줄 수 있다는 가능성에 대해서는 많은 논란이 되고 있다. 그러나 적절한 양을 섭취하는 범위에서 당의 섭취 자체만으로는 비만, 당뇨병, 대사증후군과 고콜레스테롤혈증의 위험이 증가되지 않는다는 과학적인 연구보고들이 있다. 그러므로 IOM은 "여러 자료에 근거하여, 충치, 행동적인 문제, 암, 비만의 위험성과 고지혈증의 위험성에 있어서 총 당과 첨가당에 대한 상한섭취량(tolerable upper limit)을 설정하는 것에 대한 증거가 불충분하다"고 결론을 내리고 있다.

Axisymmetric Swirling Flow Simulation of the Draft Tube Vortex in Francis Turbines at Partial Discharge

  • Susan-Resiga, Romeo;Muntean, Sebastian;Stein, Peter;Avellan, Francois
    • International Journal of Fluid Machinery and Systems
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    • v.2 no.4
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    • pp.295-302
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    • 2009
  • The flow in the draft tube cone of Francis turbines operated at partial discharge is a complex hydrodynamic phenomenon where an incoming steady axisymmetric swirling flow evolves into a three-dimensional unsteady flow field with precessing helical vortex (also called vortex rope) and associated pressure fluctuations. The paper addresses the following fundamental question: is it possible to compute the circumferentially averaged flow field induced by the precessing vortex rope by using an axisymmetric turbulent swirling flow model? In other words, instead of averaging the measured or computed 3D velocity and pressure fields we would like to solve directly the circumferentially averaged governing equations. As a result, one could use a 2D axi-symmetric model instead of the full 3D flow simulation, with huge savings in both computing time and resources. In order to answer this question we first compute the axisymmetric turbulent swirling flow using available solvers by introducing a stagnant region model (SRM), essentially enforcing a unidirectional circumferentially averaged meridian flow as suggested by the experimental data. Numerical results obtained with both models are compared against measured axial and circumferential velocity profiles, as well as for the vortex rope location. Although the circumferentially averaged flow field cannot capture the unsteadiness of the 3D flow, it can be reliably used for further stability analysis, as well as for assessing and optimizing various techniques to stabilize the swirling flow. In particular, the methodology presented and validated in this paper is particularly useful in optimizing the blade design in order to reduce the stagnant region extent, thus mitigating the vortex rope and expending the operating range for Francis turbines.

Virulence, Resistance Genes, and Transformation Amongst Environmental Isolates of Escherichia coli and Acinetobacter spp.

  • Doughari, Hamuel James;Ndakidemi, Patrick Alois;Human, Izanne Susan;Benade, Spinney
    • Journal of Microbiology and Biotechnology
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    • v.22 no.1
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    • pp.25-33
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    • 2012
  • The association of verotoxic E. coli and Acinetobacter spp. with various antibiotic-resistant, diarrhogenic, and nosocomial infections has been a cause for concern worldwide. E. coli and A. haemolyticus isolated on a number of selective media were screened for virulence factors, antibiotic resistance, and transformation of resistance genes. Out of 69 E. coli isolates obtained, 25 (35.23%), 14 (20.30%), and 28 (40.58%) were positive for Vtx1&2, Vtx1, and Vtx2, respectively, 49 (71.015%) for extendedspectrum beta-lactamases (ESBLs), 34 (49.28%) for serum resistance, 57 (82.61%) for cell surface hydrophobicity, 48 (69.57%) for gelatinase production, and 37 (53.62%) for hemolysin production. For the 14 A. haemolyticus isolates, only 2 (14.29%) in each case from all the samples investigated were positive for Vtx1, Vtx2 and Vtx1&2 respectively, 8 (57.14%) for ESBLs, 7 (50.00%) for serum resistance, 11 (78.57%) for cell surface hydrophobicity, 4 (28.57%) for gelatinase production, and 8 (57.14%) for hemolysin production. Although transformation occurred among the E. coli and Acinetobacter isolates (transformation frequency: $13.3{\times}10^{-7}-53.4^{-7}$), there was poor curing of the plasmid genes, a confirmation of the presence of stable antibiotic-resistant genes (DNA concentration between 42.7 and 123.8 ${\mu}g$) and intragenetic transfer of multidrug-resistant genes among the isolates. The isolates were potentially virulent and contained potentially transferable antibiotic resistance genes. Detection of virulence factors, antibiotic resistance genes, and transformation among these isolates is a very significant outcome that will influence approaches to proactive preventive and control measures and future investigations. However, continued surveillance for drug resistance among these bacteria and further investigation of the mechanism of action of their virulence factors are a necessity.

Impact of Clinical, Laboratory and Fluid Therapy Variables on Hospital Length of Stay for Children with Acute Pancreatitis

  • Shahein, Abdul R.;Quiros, J. Antonio;Arbizu, Ricardo A.;Jump, Candi;Lauzon, Steven D.;Baker, Susan S.
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • v.23 no.4
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    • pp.356-365
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    • 2020
  • Purpose: There have been many efforts to develop generalizable severity markers in children with acute pancreatitis (AP). Expert opinion panels have developed consensus guidelines on management but it is unclear if these are sufficient or valid. Our study aims to assess the effect of clinical and laboratory variables, in addition to treatment modality on hospital length of stay (LOS) as a proxy variable for severity in pediatric patients admitted with AP. Methods: We conducted a retrospective chart review of patients between ages of 0-18 years, who were admitted with AP at 2 institutions between 2013-2018, John R. Oishei Children's Hospital (Buffalo, NY, USA) and Medical University of South Carolina Children's Hospital (Charleston, SC, USA). We constructed three linear regression models to analyze the effect of clinical signs of organ dysfunction, laboratory markers and fluid intake on hospital LOS. Results: Ninety-two patients were included in the study. The mean age was 12 years (range, 7.6-17.4 years), 55% were females, and median LOS was 3 days. The most frequent cause of AP was idiopathic. Our study showed that elevated blood urea nitrogen (BUN) on admission (p<0.005), tachycardia that lasted for ≥48 hours (p<0.001) and need for fluid resuscitation were associated with increase LOS. Total daily fluid intake above maintenance did not have a significant effect on the primary outcome (p=0.49). Conclusion: Elevated serum BUN on admission, persistent tachycardia and need for fluid resuscitation were associated with increase LOS in pediatric AP. Daily total fluid intake above recommended maintenance did not reduce LOS.

A Mutation of cdc-25.1 Causes Defects in Germ Cells But Not in Somatic Tissues in C. elegans

  • Kim, Jiyoung;Lee, Ah-Reum;Kawasaki, Ichiro;Strome, Susan;Shim, Yhong-Hee
    • Molecules and Cells
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    • v.28 no.1
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    • pp.43-48
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    • 2009
  • By screening C. elegans mutants for severe defects in germline proliferation, we isolated a new loss-of-function allele of cdc-25.1, bn115. bn115 and another previously identified loss-of-function allele nr2036 do not exhibit noticeable cell division defects in the somatic tissues but have reduced numbers of germ cells and are sterile, indicating that cdc-25.1 functions predominantly in the germ line during postembryonic development, and that cdc-25.1 activity is probably not required in somatic lineages during larval development. We analyzed cell division of germ cells and somatic tissues in bn115 homozygotes with germline-specific anti-PGL-1 immunofluorescence and GFP transgenes that express in intestinal cells, in distal tip cells, and in gonadal sheath cells, respectively. We also analyzed the expression pattern of cdc-25.1 with conventional and quantitative RT-PCR. In the presence of three other family members of cdc-25.1 in C. elegans, defects are observed only in the germ line but not in the somatic tissues in cdc-25.1 single mutants, and cdc-25.1 is expressed predominantly, if not exclusively, in the germ line during postembryonic stages. Our findings indicate that the function of cdc-25.1 is unique in the germ line but likely redundant with other members in the soma.

A Basic Study Contributes to Extract the Standardized 3D Body Data for Women Aged 60 and Older (노년 여성 체형의 표준화된 3차원 측정 데이터 추출을 위한 기초 연구)

  • ;;Susan p. Ashdown
    • Journal of the Korean Society of Clothing and Textiles
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    • v.28 no.2
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    • pp.344-353
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    • 2004
  • The purpose of this study was to offer the basis contributes to extract the standardized body data from 3D body measuring for women aged 60 and older. The WB4 of Cyberware was used, and the measuring program of 3D scanning data was 3DM. This study was focused to verify the reliability of 3D data and to offer the effective utilization of 3D measuring on the research for elderly women■s body. Subjects were 19 women aged 60 and older. And three women in late twenties and three dressforms for women were comparing subjects to analyze the signiscant difference by age or human body variable making error. First, 3D scanning was executed twice on each subject, but any significant difference was not appear between two scanning data. So we certifed we could get the consistent and reliable data from the 3D scanner used in this study. Second, the reliability of 3D measuring data was analyzed, and the error range which meant the difference between 3D data and traditional measuring data was analyzed. In elderly women, the significant difference between two data was appeared in 19 body parts. The 7 of 19 were concerned with armpit point. In young women, three significant difference were appeared, and in dressforms, any significant difference was not certified. From these results, we could certify that age or human body variable produced the difference between two data. Third, the data of elderly women from three measuring methods, 3D measuring, traditional measuring, and measuring on 2D photographs were compared. From the result, we found that the 3D measuring data was quite reliable for most body parts excluding some width parts. But in elderly women, there were some limitation to extract reliable data because of their unique body characteristics. In order to be a role of the effective measuring method, the 3D measuring protocol reflected the body characteristics of each age or gender had to be prepared.

Photography's educational study : Against practical-based curriculum in photography education (사진의 교육적 연구 : 실기 중심 교육과정에 반하여)

  • Nam, Tack-Oon
    • Journal of Digital Convergence
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    • v.14 no.2
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    • pp.359-367
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    • 2016
  • This paper aims to develop the photography education method based, not on the practical technique, the photo recording or the communication, but on the photo theory and the visual culture. This paper can be summarized as follows: First, it analyses the photo theory itself and photography education theory. It examines the studies of the important scholars of photography theory, namely Laszlo Moholy-Nagy, Walter Benjamin, Susan Sontag. It examines also the studies of the scholars who applied photography to education, that is, Victor Burgin, Simon Watney, David Bate. This paper examines how photography is related to education theory in general and where photo education can be situated in art education field.

Physicochemical Assessment of Quality Characteristics of Extruded Barley Under Varied Storage Conditions -II. Non-enzymatic Browning- (상이한 조건하에서 저장한 압출보리의 품질특성에 관한 이화학적 평가 -제 2 보 : 비효소적 갈색화-)

  • Shin, Hyo-Sun;Gray, J. Ian;Cuppett, Susan L.
    • Korean Journal of Food Science and Technology
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    • v.15 no.3
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    • pp.287-294
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    • 1983
  • Change of color, browning index, soluble protein, reducing sugar content, and available lysine were monitored for raw and extruded barely powders during four months of ambient and accelerated storage temperatures with $A_w$ of 0.31 and 0.71, respectively. Loss of whiteness and soluble browning pigments increased with increased $A_w$ and temperature. The raw sample had an increased rate of browning intensity than the extruded samples. Among the extruded samples, the added sucrose sample had the lowest rate of browning during storage. Loss of reducing sugar content in all samples increased with increased $A_w$ and temperature. The loss of soluble protein and of the available lysine also increased with increased $A_w$ and temperature. The loss of reducing sugar and of the available lysine was at least partly due to the Maillard browning reaction. These results have important implications in teh processing and storage of raw and extruded burleys.

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A Comparison Study of Brassiere Preferences and Breast Perceptions among the Middle-aged and Older US Women

  • Kim, Hyo Sook;Kim, Ji Min;Ashdown, Susan P.
    • International Journal of Human Ecology
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    • v.15 no.2
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    • pp.33-41
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    • 2014
  • The bra is one of the most essential pieces of women's underwear which gives support for the breasts and holds them in position to make better outer appearance of upper body. Despite being worn by most women of all ages, brassiere manufacturers often use brassiere sizes and shapes that are close to the body measurements of women in their 20s, which raises questions whether the bras are suitable for older women's physique. Also, many lingerie markets tend to center on designs and tastes of younger women, leaving those of older women are largely ignored in the manufacturing process despite the fact that their purchasing power and market shares are expected to grow in conjunction with the aging of the global population. Against this backdrop, this study attempts to provide information about bra preferences and breast perceptions of the middle-aged and older US women over the age of 40 years to help manufacturers better understand the bra purchasing priorities and bra elements of older women and prompt them to design bras with a high level of fit and comfort that are appreciated by the population groups as a means to ultimately acquire competitive edge in the fast growing aging market. An email survey and interviews were conducted with 301 US women who live in New York City. The findings of this study suggest that there are internal and external factors such as changes in body and breast shapes with age influence the level of fitness and effectiveness of bra components, and thus the responses to the questions on bra preferences and breast perceptions varied between the researched age groups.