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검색결과 644건 처리시간 0.026초

Proline, Sugars, and Antioxidant Enzymes Respond to Drought Stress in the Leaves of Strawberry Plants

  • Sun, Cunhua;Li, Xuehua;Hu, Yulong;Zhao, Pingyi;Xu, Tian;Sun, Jian;Gao, Xiali
    • 원예과학기술지
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    • 제33권5호
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    • pp.625-632
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    • 2015
  • Drought is a severe abiotic stress that affects global crop production. A drought model was created for 'Toyonoka' Fragaria ${\times}$ ananassa, and the effects of drought stress on contents of proline, sugars, and antioxidant enzyme activities were investigated. Strawberry transplants with identical growth were chosen for the experiments and the randomized design included four replications (10 plants per block). The experimental sets differed in the moisture level of the culture medium relative to the range of moisture content as follows: control, 70-85%; mild drought stress, 50-60%; moderate drought stress, 40-50%; and severe drought stress, 30-40%. Drought stress was imposed by limiting irrigation. Plants were sampled and physiological parameters w ere measured on 0, 2, 4, 6, 8, and 10 days after the commencement of droughts tress. The water potential of strawberry leaves decreased in the plants under mild, moderate, and severe stress during the course of the water stress treatment and exhibited a significant difference from the control. Strawberry leaves subjected to drought stress had higher accumulation of proline, sugars, and malondialdehyde, and higher activities of superoxide dismutase, peroxidase, and catalase than leaves of control plants. Malondialdehyde levels increased in parallel with the severity and duration of drought stress. By contrast, antioxidant enzyme activity displayed dynamic responses to drought stress, first increasing and subsequently decreasing as the severity and duration of drought stress increased. These results suggest that strawberry plants respond to drought stress by altering the activities of antioxidant enzymes and the levels of osmotically active metabolites. These biochemical response changes may confer adaptation to drought stress and improve the capacity of plants to withstand water-deficit conditions.

Dihydroaustrasulfone alcohol induces apoptosis in nasopharyngeal cancer cells by inducing reactive oxygen species-dependent inactivation of the PI3K/AKT pathway

  • Kok-Tong Tan;Yu-Hung Shih;Jiny Yin Gong;Xiang Zhang;Chiung-Yao Huang;Jui-Hsin Su;Jyh-Horng Sheu;Chi-Chen Lin
    • The Korean Journal of Physiology and Pharmacology
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    • 제27권4호
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    • pp.383-398
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    • 2023
  • Dihydroaustrasulfone alcohol (DA), the synthetic precursor of a natural compound (austrasulfone) isolated from the coral species Cladiella australis, has shown cytotoxic effects against cancer cells. However, it is unknown whether DA has antitumor effects on nasopharyngeal carcinoma (NPC). In this study, we determined the antitumor effects of DA and investigated its mechanism of action on human NPC cells. The MTT assay was used to determine the cytotoxic effect of DA. Subsequently, apoptosis and reactive oxygen species (ROS) analyses were performed by using flow cytometry. Apoptotic and PI3K/AKT pathway-related protein expression was determined using Western blotting. We found that DA significantly reduced the viability of NPC-39 cells and determined that apoptosis was involved in DA-induced cell death. The activity of caspase-9, caspase-8, caspase-3, and PARP induced by DA suggested caspase-mediated apoptosis in DA-treated NPC-39 cells. Apoptosis-associated proteins (DR4, DR5, FAS) in extrinsic pathways were also elevated by DA. The enhanced expression of proapoptotic Bax and decreased expression of antiapoptotic BCL-2 suggested that DA mediated mitochondrial apoptosis. DA reduced the expression of pPI3K and p-AKT in NPC-39 cells. DA also reduced apoptosis after introducing an active AKT cDNA, indicating that DA could block the PI3K/AKT pathway from being activated. DA increased intracellular ROS, but N-acetylcysteine (NAC), a ROS scavenger, reduced DA-induced cytotoxicity. NAC also reversed the chances in pPI3K/AKT expression and reduced DA-induced apoptosis. These findings suggest that ROS-mediates DA-induced apoptosis and PI3K/AKT signaling inactivation in human NPC cells.

폭염 시 어린이공원의 온열환경 (Thermal Environments of Children's Parks during Heat Wave Period)

  • 류남형;이춘석
    • 한국조경학회지
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    • 제44권6호
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    • pp.84-97
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    • 2016
  • 본 연구는 폭염 시 어린이공원 내 포장 및 차양의 유형에 따른 온열환경을 규명하고자 하였다. 이를 위해 진주기상대에서 측정한 일 최고기온이 $35.9{\sim}36.8^{\circ}C$를 나타낸 2016년 8월 11일부터 8월 13일까지 3일간 진주시내 어린이공원 2곳(칠암어린이공원: $N\;35^{\circ}11^{\prime}1.4{^{\prime}^{\prim}}$, $E\;128^{\circ}531.7{^{\prime}^{\prime}}$, 표고: 38m; 가호제12어린이공원: $N\;35^{\circ}09^{\prime}56.8{^{\prime}^{\prime}}$, $E\;128^{\circ}6^{\prime}41.1{^{\prime}^{\prime}}$, 표고: 24m)의 모래밭, 고무칩포장지, 쉘터, 녹음지를 대상으로 미기상을 측정하였다. 미기상환경으로서 지상 60cm 높이에서 기온, 흑구온도, 상대습도, 풍속, 6방향의 장파 및 단파 복사를 측정하였고, 이를 바탕으로 열스트레스 지수인 WBGT와 UTCI를 산정 및 분석하였다. 또한 열화상카메라로 포장면과 놀이시설의 표면온도를 측정하였으며, 이를 바탕으로 단시간 피부접촉시 화상의 위험을 평가하였다. 연구결과는 다음과 같다. 어린이공원의 3일 시간 평균 최고 기온은 $36.6{\sim}39.4^{\circ}C$였으며, 모래밭에 비해 녹음에서 $2.8^{\circ}C$, 쉘터에서 $1.0^{\circ}C$$2.3^{\circ}C$ 낮게 나타났다. 시간 평균 최저 습도는 44~50%였으며, 모래밭이나 고무칩포장지에 비해 녹음에서 6%, 쉘터에서 4% 및 6% 높게 나타났다. 열사병위험지수 WBGT 값에 근거하면 폭염 시 어린이공원의 주간의 열사병 위험도는 높은 또는 극심한 정도로 나타났다. 3일 30분 평균 최고 WBGT 값은 $31.2{\sim}33.6^{\circ}C$였으며, 모래밭에 비해 녹음에서 $2.8^{\circ}C$, 쉘터에서 $2.3^{\circ}C$$1.0^{\circ}C$ 낮게 나타났으나, 차양에 의해서도 열사병 위험을 피할 수는 없었다. 체감더위지수 UTCI 값에 근거하면 폭염 시 어린이공원의 주간의 온열 스트레스의 범주는 매우 강한 또는 극심한 정도로 나타났다. 3일 30분 평균 최고 UTCI 값은 $39.9{\sim}48.1^{\circ}C$였으며, 모래밭에 비해 녹음에서 $7.8^{\circ}C$, 쉘터에서 $8.2^{\circ}C$$4.1^{\circ}C$ 낮게 나타났으나, 차양에 의해서도 극심한 또는 매우 강한 온열 스트레스를 강한 또는 적정한 온열 스트레스로 낮출 수는 없었다. 단시간 피부접촉에 의한 화상 온도 기준에 따르면, 놀이시설 및 포장면의 최고 표면온도가 스텐레스 스틸($70.8^{\circ}C$)은 무도장 철재 3초 $60^{\circ}C$, 고무칩포장($76.5^{\circ}C$)은 플라스틱 5초 $74^{\circ}C$, 청색 플라스틱 미끄럼판($68.5^{\circ}C$)과 앉음판($71.0^{\circ}C$)은 플라스틱 1분 $60^{\circ}C$ 기준을 초과한 것으로 나타났다. 하지만 그늘이 진 놀이시설의 표면온도는 햇빛에 노출된 놀이시설의 표면온도에 비해 $20^{\circ}C$ 내외로 낮게 나타나, 차양에 의해 화상의 위험을 현저하게 개선할 수 있을 것으로 판단된다. 폭염 시에는 어린이공원의 온열환경은 어린이들에게 높거나 극심한 열사병 위험에 빠지게 하고, 매우 강한 또는 극심한 온열 스트레스를 주기 때문에 보호자나 관리자가 어린이들의 어린이공원 이용을 제한해야 한다. 그리고 폭염시에는 어린이공원의 포장면 또는 놀이시설에 의한 화상의 위험이 매우 높으므로 이용 시 주의를 해야 하며, 화상의 예방을 위해서는 차양시설을 적극적으로 도입해야 한다.

Transmission Performance Comparison of Direction Detection-Based 100-Gb/s Modulation Formats for Metro Area Optical Networks

  • Chung, Hwan Seok;Chang, Sun Hyok;Lee, Jonghyun;Kim, Kwangjoon
    • ETRI Journal
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    • 제34권6호
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    • pp.800-806
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    • 2012
  • Transmission performances of direct detection-based 100-Gb/s modulation formats are investigated and compared for metro area optical networks. The effects of optical signal-to-noise ratio sensitivity, chromatic dispersion, cross-channel nonlinearity, and transmission distance on the performance of differential 8-ary phase-shift keying (D8PSK), differential phase-shift keying plus three-level amplitude-shift keying (DPSK+3ASK), and dual-carrier differential quaternary phase-shift keying (DC-DQPSK) are evaluated. The performance of coherent dual-polarization quadrature phase-shift keying (DP-QPSK) with block phase estimation and coherent DP-QPSK with digital differential detection are also presented for reference. According to our analysis, all three direct detection modulation formats could transmit a 100-Gb/s signal over several hundred kilometers of a single-mode fiber link. The results also show that DC-DQPSK outperforms D8PSK and DPSK+3ASK, and the performance of DC-DQPSK is comparable to that of coherent DP-QPSK with digital differential detection. The maximum transmission distance of DC-DQPSK is over 1,000 km, which is enough distance for metro applications.

재활심리분석시스템의 다중 우회기반 접근통제 모델 및 응용 (Indirection based Multilevel Security Model and Application of Rehabilitation Psychology Analysis System)

  • 김영수;조선구
    • 한국정보통신학회논문지
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    • 제17권10호
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    • pp.2301-2308
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    • 2013
  • 재활심리분석시스템으로서 웹이 보편적으로 사용되면서 스팸메시지가 급격히 증가하고 있다. 이의 억제를 위한 메시지통제시스템은 기술적이고 경제적 대안으로 필터시스템과 과금 시스템을 사용하고 있으나 차단오류와 과금저항으로 인한 메시지의 수신과 가용성을 제한한다. 이의 해결을 위해서 불법적인 메시지는 억제하지만 송 수신자에게 유익하고 합법적인 메시지가 폐기되고 상실되는 것을 방지할 수 있는 메시지통제시스템의 우회 모델을 제안하고 이를 사용하여 메시지의 유용성과 가용성을 높여줄 수 있는 웹기반의 메시지통제시스템을 구현하여 모델의 실용성을 검증하였다.

교차로 구간 회전 및 감속을 고려한 트랜스포터 최소 공주행 운영계획 (An Operation Scheduling of Transporters Considering Turns and Passing Delay at the Intersection Roads on the Shipyard)

  • 문종헌;유원선;조두연
    • 대한조선학회논문집
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    • 제54권3호
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    • pp.187-195
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    • 2017
  • The operation planning of transports used to move blocks is the one of key factors. Furthermore, reducing the running time through the effective plan contributes to pulling forward the whole logistic process of the shipyard and substantially saving the fuel consumption of itself as well. The past researches of the transporter focused on finding only the shortest distances, so called, Manhattan distance. However, these searching approaches cannot help having the significant difference in the real operational time and distance with the minimum cost approach which considers the speed retardation for turns or safety at the intersection. This study suggests the noble transporter's operational model which could take account of the consuming operational time around the crossroads on the shipyard. Concretely, the proposed method guarantees the minimization of transporters' turns and passage number which are huge burdensome to the operation time and the whole planning of transports with the given period. Resultantly, this paper is willing to explain the appropriateness of our approach, compared with the previous ones.

Electrochemical Immunosensing of GOx-labeled CRP Antigen on Capture Antibody Monolayer Immobilized on Calixcrown-5 SAMs

  • Jung, Hye-Sook;Song, Kum-Soo;Kim, Tai-Sun
    • Bulletin of the Korean Chemical Society
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    • 제28권10호
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    • pp.1792-1796
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    • 2007
  • Insulating effects on Au electrode according to the thickness and density of coated materials are well-known. To do electrochemical immunoassay reproducibly the glod electrode would be coated with self-assembled monolayers and antobodies. To get reproducibility, the antobody monolayer should be packed at highest density so that the amount of immobilized antibody at defined area should be the same. The calix[4]crown-5 SAMs could provide the basis for the antibodies to be immobilized reproducibly and at highest density. But the insulating effect would be highest too. We proved that the compactly packed protein monolayers on SAMs inhibited the electron transfer by block the free shuttling of redox molecules. The inhibition was minimized by inserting redox molecules in between the proteins during immobilization process. In this paper, we demonstrated that the calix[4]crown-5 SAMs would provide the protein monolayers with highest density and new method to minimize the insulating effect by inserted redox molecules in between the compactly packed protein monolayers.

주파수 청각 피드백(FAF)에 따른 말더듬 특성 연구 (A Study on Characteristics of the Stuttering according to Auditory Feedback Types)

  • 장현진;신명선
    • 한국콘텐츠학회논문지
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    • 제9권12호
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    • pp.940-947
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    • 2009
  • 본 연구는 주파수 변조 피드백(FAF) 수준에 따라 말더듬인의 말더듬 빈도와 형태 변화를 밝히고자 하였다. 발달성 말더듬 성인 10명을 대상으로 NAF와 FAF 수준별(+2000, +1000, -1000, -2000) 상황에서 읽기 및 혼자말하기 과업을 통하여 수집한 구어 표본을 분석한 결과는 다음과 같다. 읽기 및 혼자말하기 과제 모두에서 NAF와 FAF 모든 수준 간에 말더듬 빈도의 유의한 차이가 있었으나, FAF 수준 간에는 차이가 없었다. 말더듬 형태는 두 과업 모두 NAF 수준 보다 FAF 수준에서 모든 핵심행동이 감소하였다. 특히 폐쇄가 많이 감소하였고 핵심행동이 주로 반복으로 변화하였다.

Product-Story Congruence and Actor Attractiveness in Product Placements in Television Drama

  • Cheon, Minhye;Ahn, Yena;Mo, Ju Hee;Park, Jee-Sun
    • 패션비즈니스
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    • 제20권3호
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    • pp.104-118
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    • 2016
  • As more consumers avoid ads aided by advanced technologies that conveniently block ads, traditional advertising is becoming less effective than ever before. Product placements are increasingly executed to unobtrusively expose products and brands to consumers while they watch media programs. The current study attempts to enhance our understanding of the factors that affect consumer attitude towards the brands placed in TV dramas. In particular, this study examines whether the attractiveness of actors and the congruency between the products placed and the story of the TV drama influence consumer attitudes towards the TV drama and the brand. A total of 211 male consumers in their 20s and 30s were used for data analyses. Structural equation modelling revealed that the product-story congruence had a positive impact on consumer attitude towards the TV drama as well as their attitude towards the brand. Actor attractiveness (in terms of similarity and likability) positively influenced consumer attitude towards the brand. Only the perceived likability dimension of actor attractiveness was found to affect consumer attitude towards the TV drama.

빅데이터를 활용한 시니어 행동분석 돌봄 시스템 연구 (A Study on Senior Behavioral Analysis and Care System Using Big Data)

  • 장재열;최진일;어재선;최철재
    • 한국전자통신학회논문지
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    • 제15권5호
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    • pp.973-980
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    • 2020
  • 4차 산업혁명의 기술을 활용한 다양한 응용 솔루션들이 보건 복지 분야에 적용되어지고 있다. 제안한 논문에서는 빅데이터 기반의 시니어 돌봄 시스템 솔루션을 설계한다. 제안 시스템의 작동원리는 시니어의 행동분석을 스마트기기의 API정보를 통하여 수집한 후, 기존의 기준치와 다른 반응을 보일 경우에 시니어 당사자에게 1차적으로 알림을 보낸다. 만일 응답이 없을 경우에는 2차적으로 동료시니어와 가족구성원 및 응급상황센터에 정보를 제공함으로써 위험상황을 미연에 방지할 수 있는 시스템을 제안한다.