• Journal of Biomedical Engineering Research
• /
• v.26 no.3
• /
• pp.129-132
• /
• 2005

It is one of the most important issues to determine a target brain image that gives a common coordinate system for a constructing population-based brain atlas. The purpose of this study is to provide a simple and reliable procedure that determines the target brain image among the group based on the inherent structural information of three-dimensional magnetic resonance (MR) images. It uses only 11 lines defined automatically as a feature vector representing structural variations based on the Talairach coordinate system. Average characteristic vector of the group and the difference vectors of each one from the average vector were obtained. Finally, the individual data that had the minimum difference vector was determined as the target. We determined the target brain image by both our algorithm and conventional visual inspection for 20 healthy young volunteers. Eighteen fiducial points were marked independently for each data to evaluate the similarity. Target brain image obtained by our algorithm showed the best result, and the visual inspection determined the second one. We concluded that our method could be used to determine an appropriate target brain image in constructing brain atlases such as disease-specific ones.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
• /
• v.9 no.1
• /
• pp.38-44
• /
• 2016

In this paper, Author of this article presents a system to ensure the safety of residents in case the residents occurs an fall situation. Author of this article use weighted difference image and motion vector. Proposed system suggested the fall detection algorithm using weighted difference image and motion vector. Fall detection algorithm showed a success rate of 85% ~ 97.1% through 150 experiments. Proposed algorithm showed a litter higher or similar success rate than the existing camera based system.

• Proceedings of the KIPE Conference
• /
• 2007.07a
• /
• pp.403-405
• /
• 2007

This paper presents the over-modulation strategy and indirect vector control drive of NPC type PWM inverter. NPC inverter has three level phase voltage output.It can perform in high voltage through assembling switching components. It has less harmonics and surge voltage stress at motor terminals than the 2 level inverter in same switching frequency through 3 level voltage. The conventional railway vehicle has used the vector control to MI=0.907 and the slip-frequency control from MI=0.907 to six-step mode. The slip-frequency control has bad motive power and slow torque control response. But vector control has good motive power and can instant torque control. In this paper, output voltage is controlled linearly from linear region to six-step mode by using over-modulation strategy. And NPC inverter is used.

• Proceedings of the KSRS Conference
• /
• 2002.10a
• /
• pp.615-620
• /
• 2002

Probability distribution of the sea surface slope is estimated using sun glitter images derived from visible radiometer on Geostationary Meteorological Satellite (GMS) and surface vector winds observed by spaceborne scatterometers. The brightness of the visible images is converted to the probability of wave surfaces which reflect the sunlight toward GMS in grids of 0.25 deg $\times$ 0.25 deg. Slope and azimuth angle required for the reflection of the sun's ray toward GMS are calculated for each grid from the geometry of GMS observation and location of the sun. The GMS images are then collocated with surface wind data observed by three scatterometers. Using the collocated data set of about 30 million points obtained in a period of 4 years from 1995 to 1999, probability distribution function of the surface slope is estimated as a function of wind speed and azimuth angle relative to the wind direction. Results are compared with those of Cox and Munk (1954a, b). Surface slope estimated by the present method shows narrower distribution and much less directivity relative to the wind direction than that reported by Cox and Munk. It is expected that their data were obtained under conditions of growing wind waves. In general, wind waves are not always developing, and slope distribution might differ from the results of Cox and Munk. Most of our data are obtained in the subtropical seas under clear-sky conditions. This difference of the conditions may be the reason for the difference of slope distribution.

• Reproductive and Developmental Biology
• /
• v.31 no.3
• /
• pp.153-159
• /
• 2007

In this study, using FIV-based lentivirus vector system, we tried to express hG-CSF in tetracycline-controllable manner. hG-CSF influences the proliferation, differentiation, and survival of cells in the neutrophil lineage. To enhance stability and translation of hG-CSF transcript, WPRE sequence was also introduced into FIV-Tet-On vector at downstream region of either the hG-CSF gene or the sequence encoding rtTA. Primary culture cells (CEF, chicken embryonic fibroblast; PFF, procine fetal fibroblast) infected with the recombinant FIV were cultured in the medium supplemented with or without doxycycline for 48 hours, and induction efficiency was measured by comparing the hG-CSF gene expression level using quantitative real-time PCR, Western blot and ELISA. Higher hG-CSF expression and tighter expression control were observed from the vector in which the WPRE sequence was placed at downstream of the hG-CSF (in CEF) or rtTA (in PEE) gene. This FIV-Tet-On vector system may be helpful in solving serious physiological disturbance problems which has continuously hampered successful production of transgenic animals and gene therapy.

• Asian-Australasian Journal of Animal Sciences
• /
• v.14 no.2
• /
• pp.163-169
• /
• 2001

Despite the high potency of the retrovirus vector system in gene transfer, one of the main drawbacks of has been difficulty in preparing highly concentrated virus stock. Numerous efforts to boost the virus titer have ended in unsatisfactory results mainly due to fragile property of retrovirus envelope protein. In this study, to overcome this problem, we constructed our own retrovirus vector system producing vector viruses encapsulated with VSV-G (vesicular stomatitis virus G glycoprotein). Concentration process of the virus stock by ultracentrifuge did not sacrifice the virus infectivity, resulting in more than 108 to 109 CFU (colony forming unit) per ml on most of the target cell lines tested. Application of this high-titer retrovirus vector system was tested on chicken embryos. Injection of virus stock beneath the blastoderms of pre-incubated fertilized eggs resulted in chick embryos expressing E. coli LacZ gene with 100% efficiency. Therefore, our results suggest that it is possible to transfer the foreign gene into chicken embryo using our high-titer retrovirus vector.

• Reproductive and Developmental Biology
• /
• v.29 no.1
• /
• pp.57-62
• /
• 2005

One of the critical problems to be solved in transgenic animal production is uncontrollable constitutive expression of foreign genes, which usually results in serious physiological disturbances in the transgenic animal. To circumvent this problem, we constructed and tested two retrovirus vectors designed to express the GFP(green fluorescent protein) gene under the control of the tetracycline-inducible promoters. To maximize the GFP gene expression at turn-on state, WPRE(woodchuck hepatitis virus posttranscriptional regulatory element) sequence was introduced into the retrovirus vectors at downstream region of either the GFP gene or the sequence encoding rtTA(reverse tetracycline-controlled transactivator). Transformed cells were cultured in the medium supplemented with or without doxycycline(tetracycline derivative) for 48 hours, and induction efficiency was measured by comparing the GFP gene expression level using fluorometry and western blotting. Higher GFP expression was observed from the vector carrying the WPRE sequence at 3' side of the GFP gene, while tighter expression control(up to 20 fold) was obtained from the vector in which the WPRE sequence was placed at 3' side of rtTA sequence. The resulting tetracycline inducible vector system may be used in transgenic animal production and gene therapy.

• Proceedings of the Korean Society of Plant Biotechnology Conference
• /
• 2005.11a
• /
• pp.285-285
• /
• 2005

• Proceedings of the Korean Institute of Communication Sciences Conference
• /
• 2004.11a
• /
• pp.86-86
• /
• 2004

• Proceedings of the Zoological Society Korea Conference
• /
• 1999.10b
• /
• pp.290.1-290
• /
• 1999

No Abstract, See Full Text