• Parasites, Hosts and Diseases
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• v.37 no.2
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• pp.121-125
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• 1999

Inoculation of human fecal cysts to suckling Mongolian gerbils, two Giardia lamblia isolates, Kl and K2, were established as axenic cultures. Using this in vitro culture, both two Giardia isolates were grouped by using two genetic analysis. With genetic analysis of SSS-rDNA sequences, both K1 and K2 were found as members of hopkins'group 1, despite some nucleotide differences noticed in K1 (5 differences/292 bases.). The other genetic study used PCI-RFLP of the tim (triose phosphate isomerase) Nash's group 2 can bot be a separate group, but a part of Hopkins' group 1.

• Korean Journal of Veterinary Research
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• v.20 no.1
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• pp.33-38
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• 1980

1. In adult ICR-SLC mice, significant increase in the occurrence of the Tyzzer's disease was noted when the reticuloendothelial system was blockaded with the carbon particles. The most suitable bloakading time was 2 hours before inoculating Tyzzer's organisms, the route was intraperitoneal and the dose was 3mg/body. 2. Survival rate of the experimental group ICR-SLC suckling mice receiving BCG ($3{\times}10^8$) 6 days prior to intraperitoneal inoculation of Tyzzer's organisms was 80 percent, but the control group was 17%. 3. In comparing the survival rate of the nude/nude mice with the nude/+ mice, the former congenitally thymus deficient mice are 11 percent, but the latter are 67 percent.

• The Journal of the Korean Society for Microbiology
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• v.21 no.2
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• pp.227-231
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• 1986

The hemagglutination activities of Hantaan virus and Seoul virus were demonstrated. The hemagglutinins were prepared by sucrose-acetone extraction method from suckling mouse brains infectecd with Hantaan and Seoul viruses. Hemagglutination of goose erythrocytes by these viral hemagglutinins was pH dependent in phosphate buffer system. Hantaan and Seoul viruses were distinguished by pH range of hemagglutination. 76/118 and 79/90 strains of Hantaan virus showed hemagglutination at the range of pH 5.75-6.4 and the optimal pH was 5.75 with the titer of 1:512 in 76/118 and 1:256 in 79/90. In contrast, KSNUSD 84/34 strain of Seoul virus revealed hemagglutination at the range of pH 6.2-6.4 and the optimal pH was 6.4 with the titer of 1 : 64.

• IMMUNE NETWORK
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• v.16 no.3
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• pp.195-199
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• 2016

Fecal samples obtained from wild boar habitats are useful for the surveillance of diseases in wild boar populations; however, it is difficult to determine the species of origin of feces collected in natural habitats. In this study, a fecal IgA ELISA was evaluated as a method for identifying the porcine species from fecal samples. Both domestic pigs (Sus scrofa domestica) and wild boars (Sus scrofa coreanus) showed significantly higher levels of fecal IgA than other animal species. Additionally, age dependent changes in the level of Ig A in wild boars and domestic pigs were identified; Titers of Ig A were highest in suckling period and lowest in weanling period.

• Journal of agriculture & life science
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• v.44 no.5
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• pp.55-64
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• 2010

This study was conducted to estimate genetic parameters for reproductive traits using multivariate animal models in Yorkshire breed. For the study, 4,989 records for litter traits collected between the year 2001 and 2005 from Yorkshire pigs in K GGP were used. The effects of environmental factors such as farrowing year, parity, weaning to estrus interval (WEI), and suckling period were statistically significant (p<0.05), but farrowing season was not significant, for reproductive traits. The estimates genetic correlations and phenotypic correlations in total number of born and number of suckling, was shown to highly correlated. The genetic correlations were higher than phenotypic correlation. The estimates of heritabilities for reproductive traits, considering permanent environment effects (PE) were much lower than those obtained when permanent environment effects were not considered (NPE) in the model. The estimates of heritabilities were 0.240 and 0.076 for total number of born and 0.187 and 0.096 for number of suckling in NPE, and PE, respectively. These results itivcate that PE should be considered in the statistical mode to estimate more acco ate breeding values.

• Journal of agriculture & life science
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• v.44 no.3
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• pp.41-52
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• 2010

This study was conducted to estimate genetic parameters for growth traits using multivariate animal models in Yorkshire breed. For the study, 16,202 records for growth traits collected between the year 1999 and 2005 from Yorkshire pigs in K GGP were used. The effects of environmental factors such as sex, birth year, birth season, parity and birth weight group affected growth traits significantly (p<0.01). Birth weight tended to be positively correlated with average daily gain (ADG) and lean percent. But it seemed to affect age at 90 kg, average adjusted backfat thickness (BF), and eye muscle ares (EMA) negatively. For average pig suckling weight (ASW) and total weight at suckling (TWS), the higher birth weight is the better performance. But, in case of total number of born and number of suckling, the result was shown vice versa. Approximately 10~30% lower heritability estimates were obtained for growth traits by using the model that includes descriptions of common litter effects (CL) than by using the model that ignores those (NCL) for more accurate estimation of heritability. The estimates of heritabilities were 0.468, and 0.328 for ADG, 0.474 and 0.326 for age at 90 kg, 0.452, and 0.396 for BF, 0.240 and 0.200 for EMA and, 0.458, and 0.380 for lean percent in NCL and CL, respectively. Therefore, in order to estimate optimal genetic parameters, it could be inferred that the statistical model which considers litter effects must be applied.

• Journal of Animal Science and Technology
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• v.56 no.3
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• pp.11.1-11.9
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• 2014

This study was conducted to evaluate the effects of dietary conjugated linoleic acid (CLA) on the performance of lactating sows and piglets as well as the immunity of piglets suckling from sows fed CLA. Eighteen multiparous Duroc sows with an average body weight (BW) of $232.0{\pm}6.38kg$ were randomly selected and assigned to two dietary treatments (n = 9 for each treatment), control (no CLA addition) and 1% CLA supplementation. For the control diet, CLA was replaced with soybean oil. Experimental diets were fed to sows during a 28-day lactation period. Litter size for each sow was standardized to nine piglets by cross-fostering within 24 hours after birth. Sow milk and blood samples were taken from sows and piglets after 21 and 27 days of lactation, respectively. Loss of BW was significantly (p < 0.05) higher in sows fed control diet compared to sows fed CLA diet. Piglet weights at weaning and weight gain during suckling were significantly (p < 0.05) higher in sows fed CLA compared to sows fed control diet. Serum non-esterified fatty acid (NEFA) and urea nitrogen concentrations were significantly (p < 0.05) lower in sows fed CLA than in sows fed soybean oil. IgG concentrations of the groups supplemented with CLA increased by 49% in sow serum (p < 0.0001), 23% in milk (p < 0.05), and 35% in piglet serum (p < 0.05) compared with the control group. Sows fed CLA showed an increase of 10% in milk yield compared with sows fed soybean oil (p < 0.05), even though there was no difference in daily feed intake between the treatments. Milk fat content was significantly (p < 0.05) lower in sows fed CLA than in sows fed soybean oil. Solid-not-fat yield was significantly (p < 0.05) higher in sows supplemented with CLA than in sows fed control diet and also protein-to-fat ratio in milk was significantly (p < 0.05) higher in sows fed CLA compared with the control group. The results show that CLA supplementation to sows increased milk yield without losing BW during lactation, whereas soybean oil supplementation resulted in severe BW loss.

• The Journal of Korean Society of Virology
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• v.27 no.1
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• pp.39-47
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• 1997

A large number of viruses belonging to Genus Hantavirus in Family Bunyaviridae are etiologic agents for hemorrhagic fever with renal syndrome (HFRS), or hantavirus pulmonary syndrome (HPS). Hantaan (HTN), Seoul (SED), Belgrade (BEL), Puumala (PUU) serotype viruses are well known causative agents for HFRS in Eurasian continent. Among those viruses Hantaan and Seoul serotypes are well known to cause HFRS in Korea, but there are some sporadic incidence by other than Hantaan or Seoul viruses. Recently we have developed the combined Hantaan-Puumala virus vaccine to prevent world-wide occuring HFRS. This combined vaccine is formalin inactivated, suckling mouse and suckling hamster brain extracts for Hantaan and Puumala viruses, respectively. Protein contents of this purified candidate vaccine is $27\;{\mu}g/ml$, which contains 1,024 ELISA antigen units to each virus, but content of myelin basic protein which is causing experimental allergic encephalomyelitis is less than 0.1 ng/ml. Thirty hamsters were given twice at one month interval intra-muscularly and bled on 30 days after each vaccination from retro-orbital sinus vein. Antibody titers were tested against 5 major serotype viruses, Hantaan, Seoul, Belgrade, Puumala and Sin Nombre viruses by IFA and PRNT. The mean IF antibody titers on 30 days after primary shot were 78.4, 68.8, 68.8, 37.9, and 15.6; mean neutralizing antibody titers were 65.4, 12, 6.1, 65.6 and 0.5 against Hantaan, Seoul, Belgrade, Puumala and Sin Nombre viruses, respectively. The mean IF antibody titers on 30 days after booster shot were 686.9, 567.5, 550.4, 516.3, and 430.9; and neutralizing antibody titers were 710.8, 41.9, 24.3, 409.9, and 1.6 against Hantaan, Seoul, Belgrade, Puumala and Sin Nombre viruses, respectively.

• Korean Journal of Veterinary Service
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• v.26 no.1
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• pp.73-80
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• 2003

In order to obtain information on murine model for foot and mouth disease virus(FMDV) type Asia 1, we studied whether guinea pig was a suitable model for studying FMDV. Apparently healthy 3 months old albino guinea pigs and unweaned 3 days old Swiss albino mice were used for this study. Total of 8 guinea pigs were divided into the infected(n=5) and control(n=3) groups. The incubation period of FMDV in the guinea pigs were roughly 2 days and the viremia persisted for 3 days in the guinea pigs. Mice inoculated with the plasma from control guinea pigs did not show any sign of viremia. The plasma were titrated by virus neutralization test using suckling mice as an indicator host. The mean virus neutralizing antibody titers of infected guinea pig at 3 DPI, 4 DPI and 5 DPI were log$\_$10/2.16, log$\_$10/ 3.39 and log$\_$10/ 3.44, respectively whereas there was no neutralizing antibody titer in control group. The difference between the mortality pattern and mean virus neutralizing antibody titer of infected and that of control group at day 3, 4, 5 were statistically significant(p<0.0l).

• Journal of Veterinary Clinics
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• v.24 no.4
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• pp.588-592
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• 2007

The prevalence of E. coli(K99), Clostridium perfringens and Cryptosporidium parvum on acute diarrhea in suckling Korean native calves was evaluated in the field by a veterinary practice. In diagnosis, fecal samples were directly collected from calves that had diarrhea between 2 and 98 days of age. 40 samples were analyzed in October, 2006 and December, 2006. Clostridium perfringens and Cryptosporidium parvum were detected in 15(37.5%) and 4(10.0%) of the samples from diarrhetic calves, respectively. However, E. coli(K99) was not detected in the samples from diarrhetic calves. There was no significant difference(p>0.05) between October(5, 25.0%) and December(10, 50.0%) in incidence of detected Clostridium perfringens from diarrhetic calves. On the other hand, significant differences (p<0.05) in the detection rate of Clostridium perfringens were found between the within 1 month age and all other age groups. In the detection of Cryptosporidium parvum, there was no significant difference(p<0.05) between October (2, 10.0%) and December(2, 10.0%) in the incidence of detected Cryptosporidium parvum from diarrhetic calves. These results suggest that causative agents of calf diarrhea occurred frequently with Clostridium perfringens infection than E. coli(K99) and Cryptosporidium parvum.