Parasites, Hosts and Diseases
- Volume 37 Issue 2
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- Pages.121-125
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- 1999
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- 2982-5164(pISSN)
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- 1738-0006(eISSN)
Axenic cultivation and characterization of Giardia lamblia isolated from humans in Korea
- Park, Soon-Jung (Department of Parasitology and Institute of Tropical Medicine, Yonsei University College of Medicine) ;
- Yong, Tai-Soon (Department of Parasitology and Institute of Tropical Medicine, Yonsei University College of Medicine) ;
- Yang, Hye-Won (Department of Parasitology and Institute of Tropical Medicine, Yonsei University College of Medicine) ;
- Lee, Du-Ho (Department of Parasitology and Institute of Tropical Medicine, Yonsei University College of Medicine) ;
- Lee, Kyung-Won (Department of Clinical Pathology, Yonsei University College of Medicine)
- Published : 1999.06.01
Abstract
Inoculation of human fecal cysts to suckling Mongolian gerbils, two Giardia lamblia isolates, Kl and K2, were established as axenic cultures. Using this in vitro culture, both two Giardia isolates were grouped by using two genetic analysis. With genetic analysis of SSS-rDNA sequences, both K1 and K2 were found as members of hopkins'group 1, despite some nucleotide differences noticed in K1 (5 differences/292 bases.). The other genetic study used PCI-RFLP of the tim (triose phosphate isomerase) Nash's group 2 can bot be a separate group, but a part of Hopkins' group 1.