• BMB Reports
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• v.53 no.10
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• pp.539-544
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• 2020

Skin aging appears to be the result of overlapping intrinsic (including genetic and hormonal factors) and extrinsic (external environment including chronic light exposure, chemicals, and toxins) processes. These factors cause decreases in the synthesis of collagen type I and elastin in fibroblasts and increases in the melanin in melanocytes. Collagen Type I is the most abundant type of collagen and is a major structural protein in human body tissues. In previous studies, many products containing collagen derived from land and marine animals as well as other sources have been used for a wide range of purposes in cosmetics and food. However, to our knowledge, the effects of human collagen-derived peptides on improvements in skin condition have not been investigated. Here we isolate and identify the domain of a human COL1A2-derived protein which promotes fibroblast cell proliferation and collagen type I synthesis. This human COL 1A2-derived peptide enhances wound healing and elastin production. Finally, the human collagen alpha-2 type I-derived peptide (SMM) ameliorates collagen type I synthesis, cell proliferation, cell migration, and elastin synthesis, supporting a significant anti-wrinkle effect. Collectively, these results demonstrate that human collagen alpha-2 type I-derived peptides is practically accessible in both cosmetics and food, with the goal of improving skin condition.

• Proceedings of the Korean Society of Applied Pharmacology
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• 2000.04a
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• pp.10-14
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• 2000

The cycloooxygenase enzymes catalyze the oxidative conversion of arachidonic acid into prostag1andin H$_2$Which mediates both benificial and pathological effects. The COX-1 is constitutively expressed in most tissues and in blood platelets wherease the expression of COX-2 isoform is induced in response to inflmmatory stimuli such as cyctokynes. Thus the identification of a novel COX-2 selective inhibitor should offer excellent antiinflammatory activity with minimal side effects such as gastrointestinal toxicity. Recently, a group of structurally unique and biologically active pimarane diterpenoids has been isolated from indigenous Korean medicinal plants. These new diterpenoids turned out to be potential analgesic and antiinflammatory agent due to their potent inhibitory activities of prostaglandin synthesis. We have also found that the inhibition of PGE$_2$synthesis is attributed to the potent COX inhibition by pimarane diterpenoid in arachidonic acid cascade. In conjunction with development of new analgesic and nonsteroidal antiinflammatory agent, a series of works on these diterpenoids have been extensively carried out in our laboratories. These efforts involve the structure-activity relationship of pimaradienoic acid, molecular modelings and COX inibitory activities as well as actiinflammatory effects of its structural analogues. In addition, the total syntheses of the new natural pimarane diterpenoids, their stereoisomers and other structural variants were intensively investigated.

• Computational Structural Engineering
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• v.4 no.1
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• pp.109-120
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• 1991

This paper introduces a geometric modelling system adopted in a newly developed preprocessor for finite element analysis of three dimensional structures. The formulation is characterized by hierachical construction of structural model which consists of control points, curves, surfaces and solids. Various surface and solid modelling schemes based on blending functions and boundary representation are systematized for finite element mesh generation. The modelling system is integrated with model synthesis and operations which facilitate modelling of complex structures.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.20 no.12
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• pp.3782-3791
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• 1996

A great deal of effert has been invested in upgrading the performance and the efficiency of mechanical structures. Using experimental modal analysis(EMA) or finite element analysis(FEA) data of mechanical structures, this performance and efficiency can be effectively evaluated. In order to analyze complex structures such as automobiles and aircraft, for the sake of computing efficiency, the dynamic substructuring techniques that allow to predict the dynamic behavior of a structure based on that of the composing structures, are widely used. By llinking a modal model obtained from EMA and an analytical model obtained from FEA, the best conditioned structures can be desinged. In this paper, a new algorithm for structural dynamic modification-SRFSM (substructure response function sensitivity method) is proposed by linking frequency responce function synthesis and response function sensitivity. A mehtod to obtain response function sensitivity using direct derivative of mechanical impedance, is also used.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 1997.10a
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• pp.364-369
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• 1997

It is very difficult to execute the vibration analysis of a huge structure, which takes up much time and expense. In this paper we intend to make the equivalent system of a local vibration system of a huge structure with a view to improving the dynamic characteristics and reducing time and expense. First of all, upper deck structure model is maded. And we perform the vibration analysis by the Substructure Synthesis Method and execute the exciting test for the upper deck structure model, and observe the coincidences of two results to confirm the reliability of the analyzing tools used. To make the equivalent system, we give boundary condition to sub-structure that want to be modified and execute the Sensitivity Analysis Method and the Optimum Structural Modification Method. And we execute the structural modification of the equivalent system.

• Journal of Ship and Ocean Technology
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• v.5 no.1
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• pp.14-29
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• 2001

Concept and preliminary structural design methods, including large scale FEM analysis and optimisation, for large passenger ships, car passenger and RoRo/ passenger ships are dis-cussed. Applications and experiences in practical design usage are presented.

• Natural Product Sciences
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• v.4 no.3
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• pp.136-142
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• 1998

Atratoxin $B_1$ which was found to inhibit the growth of Candida albicans caused structural and morphological alteration of the cells. Increased accumulation of vesicles and membranous bodies in the cytoplasm, and alterations of the cell membrane and cell wall were most obvious. Sequential lytic events of the cells eventually resulted in complete disintegration of the cytoplasmic structures. These results suggested that atratoxin $B_1$ functioned by either blocking the biosynthetic step during cell wall synthesis, altering cell wall metabolism or dissolution of the cell organelles. These changes caused a progressive destruction of the cell wall leading to cell lysis.

• Smart Structures and Systems
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• v.33 no.5
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• pp.349-358
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• 2024

Structural digital models can be effectively established by spatially obtaining digital images using an unmanned aerial vehicle (UAV). One of the main purposes of the structural digital modeling is computer vision-based exterior damage detection of a target structure. To investigate micro-scale damage from the digital model, high-resolution digital images obtained with a close-up vision survey is typically required. However, serial image synthesis such as image stitching may cumulate stitching errors as the number of scanned images increases. Therefore, in this paper, a novel loop closure-based digital image stitching technique is proposed and experimentally validated using the close-up surveyed digital images acquired from an in-situ dam structure located in South Korea. The test results reveal that the proposed technique outperforms a non-loop closure-based image stitching technique, which can cause serious distortions, such as ghosting and vanishing phenomena.

• Journal of Microbiology and Biotechnology
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• v.15 no.4
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• pp.767-771
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• 2005

Expression in yeast may prove more amenable to generating large amounts of viral antigens for a vaccine candidate. We, therefore, cloned the gene encoding the Hepatitis C virus (HCV) structural proteins (C-El-E2, c740) fused in-frame with, and immediately 3' to, the chicken-lysozyme signal peptide (C-SIG) gene and under the control of the yeast glyceraldehyde-3-phosphate dehydrogenase gene promoter. In yeast, the HCV structural proteins were expressed in two different forms: a processed and a nonprocessed aggregated form. Biophysical characterization by sucrose linear gradient centrifugation revealed that both forms were present in the same fractions with a buoyant density of 1.127-1.176 g/$cm^3$. These findings suggest that the efficient synthesis of HCV structural proteins in yeast may be an important tool to study virus assembly and may lead to the development of an HCV vaccine.

• Proceedings of the Korean Vacuum Society Conference
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• 2013.08a
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• pp.202.2-202.2
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• 2013

Ti2O3 is known as a typical Mott insulator with a transition temperature of near $200^{\circ}C$. Unlike VO2, Ti2O3 does not have a structural phase transition near the metal-insulator-transition (MIT) temperature. We investigated the temperature-dependent thermal vibration change using temperature-dependent x-ray absorption fine structure (XAFS) at Ti K-edge in the temperature range of 300~600 K. Ti2O3 powder and films were synthesized using thermal chemical vapor deposition (CVD) at $800{\sim}900^{\circ}C$. X-ray diffraction measurements show a single phased Ti2O3 at room temperature. XAFS confirmed no structural phase transition in the temperature of 300~600 K. A small but distinguishable structural disorder change was observed near the transition temperature. We will discuss the MIT behavior with the change of structural disorder.