• BioChip Journal
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• 제12권4호
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• pp.287-293
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• 2018

Bacillus cereus can cause blood infections (i.e., sepsis). Its early detection is very important for treating patients. However, an antibody with high binding affinity to B. cereus is not currently available. Bacteriophage cell wall-binding domain (CBD) has strong and specific binding affinity to B. cereus. Here, we report the improvement in the sensitivity of an ATP bioluminescence assay for B. cereus detection using CBD-conjugated magnetic nanoparticles (CBD-MNPs). The assay was able to detect as few as 10 colony forming units (CFU) per mL and $10^3CFU\;per\;mL$ in buffer and blood. CBD-MNPs did not show any cross-reactivity with other microorganisms. These results demonstrate the feasibility of the ATP assay for the detection of B. cereus.

• Earthquakes and Structures
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• 제16권4호
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• pp.425-436
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• 2019

Successive ground motions having short time intervals have occurred in many earthquakes so far. It is necessary to investigate the effects of this phenomenon on different types of structures and to take these effects into consideration while designing or retrofitting structures. The effects of seismic sequences on the structures with combined reinforced concrete shear wall and moment resisting frame system have not been investigated in details yet. This paper has tried to analyse the seismic performance of structures with such structural systems subjected to mainshock-aftershock sequences. The effects of the seismic sequences on the investigated models are evaluated by strong measures such as IDA capacity and fragility and vulnerability curves. The results of this study show that the seismic sequences have a significant effect on the investigated models, which necessitates considering this effect on designing, retrofitting, decision making, and taking precautions.

• Structural Engineering and Mechanics
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• 제54권4호
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• pp.741-754
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• 2015

This study evaluates prediction models for three EDPs (engineering demand parameters) using data from three symmetrical structures with RC walls designed according to the currently enforced Romanian seismic design code P100-1/2013. The three analyzed EDPs are: the maximum interstorey drift, the maximum top displacement and the maximum shear force at the base of the RC walls. The strong ground motions used in this study consist of three pairs of recordings from the Vrancea intermediate-depth earthquakes of 1977, 1986 and 1990, as well as two other pairs of recordings from significant earthquakes in Turkey and Greece (Erzincan and Aigion). The five pairs of recordings are rotated in a clockwise direction and the values of the EDPs are recorded. Finally, the relation between various IMs (intensity measures) of the strong ground motion records and the EDPs is studied and two prediction models for EDPs are also evaluated using the analysis of residuals.

• International Journal of Fluid Machinery and Systems
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• 제4권3호
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• pp.349-359
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• 2011

An unsteady numerical analysis has been carried out to study the strong impeller volute interaction of a centrifugal pump with six backward swept blades shrouded impeller. The numerical analysis is done by solving the three-dimensional Reynolds Averaged Navier-Stokes codes with standard k-${\varepsilon}$ two-equations turbulence model and wall regions are modeled with a scalable log-law wall function. The flow within the impeller passage is very smooth and following the curvature of the blade in stream-wise direction. However, the analysis shows that there is a recirculation zone near the leading edge even at design point. When the flow is discharged into volute casing circumferentially from the impeller outlet, the high velocity flow is severely distorted and formed a spiraling vortex flow within the volute casing. A spatial and temporal wake flow core development is captured dynamically and shows how the wake core diffuses. Near volute tongue region, the impeller/volute tongue strong interaction is observed based on the periodically fluctuating pressure at outlet. The results of existing analysis also proved that the pressure fluctuation periodically is due to the position of impeller blade relative to tongue.

• Food Science and Biotechnology
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• 제15권6호
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• pp.877-883
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• 2006

The immunostimulatory activities of Lactobacillus plantarum, the major microorganism in kimchi fermentations were investigated. Five strains of L. plantarum exhibited weak immunopotentiating activity, but L. plantarum PS-21 showed as strong a mitogenic activity as Bifidobacterium adolescentis M101-4, a known positive strain. It is of interest that, L. plantarum PS-21 stimulated proliferation of Peyer's patch cells, one of the most important tissues in the gut-associated lymphoreticular system. Cell' wall fractions from L. plantarum PS-21 also showed strong mitogenic activity compared with the soluble cytoplasmic fraction. A peptidoglycan fraction (PG) extracted from the cell wall of L. plantarum PS-21 was identified as an active mitogenic component when used in murine lymph node and spleen cell test systems. PG showed dose-dependent mitogenic activity and significantly enhanced antibody production in lymph node cells when studied in vitro. The lysosomal enzyme activity of murine peritoneal macrophages was increased when analyzed following injection of PG to the host animal. Furthermore, PG enhanced the production of cytokines such ($TNF-{\alpha}$ and IL-6) in the in vitro culture of RAW 264.7 macrophage cells.

• 대한기계학회논문집B
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• 제25권3호
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• pp.405-413
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• 2001

The present study investigates convective heat/mass transfer and flow characteristics inside a rotating two-pass rectangular duct. A naphthalene sublimation technique is employed to determine the detailed local heat transfer coefficients using the heat and mass transfer analogy. The objective of this study is to determine the effects of turning geometry with rotation for 0.0$\leq$Ro$\leq$0.24. The results reveal that the sharp-turn corner has the larger pressure drop and lower heat transfer in the post-turn region than those of the round-turn corner. The strong secondary flow enhances heat transfer for the round-turn corner. Coriolis force induced by the rotation pushes the high momentum core flow toward the trailing wall in the first passage with radially outward flow and toward the leading wall in the second passage with radially inward flow. Consequently, the high heat transfer rates are generated on the trailing surface and the leading surface in the first and second passage, respectively. However, the strong secondary flow due to the turning dominates the flow pattern in the second passage, thus the heat transfer differences between the leading and trailing surfaces are small with the rotation.

• Earthquakes and Structures
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• 제21권5호
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• pp.477-487
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• 2021

This study focuses on the influence of strong ground motion duration on the response and collapse probability of reinforced concrete walls with a predominant response in flexure. Walls with different height and mass were used to account for a broad spectrum of configurations and fundamental periods. The walls were designed following the specifications of the Chilean design code. Non-linear models of the reinforced concrete walls using a distributed plasticity approach were performed in OpenSees and calibrated with experimental data. Special attention was put on modeling strength and stiffness degradation. The effect of duration was isolated using spectrally equivalent ground motions of long and short duration. In order to assess the behavior of the RC shear walls, incremental dynamic analyses (IDA) were performed, and fragility curves were obtained using cumulative and non-cumulative engineering demand parameters. The spectral acceleration at the fundamental period of the wall was used as the intensity measure (IM) for the IDAs. The results show that the long duration ground motion set decreases the average collapse capacity in walls of medium and long periods compared to the results using the short duration set. Also, it was found that a lower median intensity is required to achieve moderate damage states in the same medium and long period wall models. Finally, strength and stiffness degradation are important modelling parameters and if they are not included, the damage in reinforced concrete walls may be greatly underestimated.

• 한국가시화정보학회지
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• 제21권3호
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• pp.75-84
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• 2023

In this study, we conducted analysis of bubble dynamics and flow of liquid phase change material(PCM) using shadowgraphy and particle image velocimetry(PIV). Characteristics of internal flow varied depending on locations of injection when solid PCM was liquefied from heated vertical wall. When bubbles rose immediately, they exhibited elliptical shape and zigzag trajectory. In contrast, when bubbles rose after merging at the bottom of solid PCM, with equivalent diameter for the inter-wall distance of 0.64 or greater, they showed a jellyfish shape and strong rocking behavior. It was observed by the PIV that the small ellipse bubbles made most strong flow inside the liquid PCM. Furthermore, the flow velocity was highest in the case of front injection, as the directions of temperature gradients and bubble-driven flow were aligned. The results underscore the significant influence of injection location on various characteristics, including bubble size, shape, rising path of bubbles, and internal flow.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2001년도 하계학술대회 논문집
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• pp.237-242
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• 2001

The stochiometric mix of evaporating materials for the CuGaSe$_2$ single crystal thin films was prepared from horizontal furnace. To obtain the single crystal thin films, CuGaSe$_2$ mixed crystal was deposited on thoroughly etched semi-insulating GaAs(100) substrate by the Hot Wall Epitaxy (HWE) system. The source and substrate temperature were 610$^{\circ}C$ and 450$^{\circ}C$, respectively The crystalline structure of single crystal thin films was investigated by the photoluminescence and double crystal X-ray diffraction (DCXD). From the photocurrent spectrum by illumination of perpendicular light on the c-axis of the CuGaSe$_2$ single crystal thin film, we have found that the values of spin orbit splitting Δ So and the crystal field splitting ΔCr were 91 meV and 249.8 meV at 20 K, respectively. From the Photoluminescence measurement on CuGaSe$_2$ single crystal thin film, we observed free excition (Ex) existing only high quality crystal and neutral bound exiciton (D$^{\circ}$,X) having very strong peak intensity. Then, the full-width-at-half-maximum(FWHM) and binding energy 7f neutral acceptor bound excision were 8 meV and 35.2 meV, respectivity. By Haynes rule, an activation energy of impurity was 355.2 meV

• 한국태양에너지학회 논문집
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• 제24권2호
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• pp.25-31
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• 2004

Ventilation through air vent system in a building envelope is expected to be an effective measure to release solar radiation. An external surface of a wall absorbs solar radiation and transfers it to the air in the cavity. The warmed air gets buoyant force. So when openings are provided at the top and bottom of the cavity, the warmed air is released through the top opening and cooler outside air replaces the space in the cavity. This reduces the further heat transmission into the built environment. This natural ventilation effect seems to be steady and strong. So because of the cavity and the openings, the cooling load reduction by natural ventilation is believed to be considerable.