• Journal of Life Science
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• v.21 no.3
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• pp.400-405
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• 2011

Autophagy, a highly conserved mechanism of internal quality control, is essential for the maintenance of cellular homeostasis and for the orchestration of an efficient cellular response to stress. During aging, the efficiency of autophagic degradation declines and intracellular waste products accumulate. Therefore, the aim of this study is to investigate the effects of exercise on autophagic response in skeletal muscle. Twenty-four Young (4 month) and Old (12 month) ICR-type white male mice were divided into a control group (CON: n=6) and exercise training group (Tr: n=6) after an adaptation period of 1 week. Exercise consisted of treadmill running at 16.4 m/min with a 4% incline, 40 min/day and 5 days/week for 8 weeks. Cervical dislocation was performed at 48 hours after the last round of exercise, after which the gastrocnemius skeletal muscle were immediately collected. The results of verifying autophagy formation showed that the Sarcopenia index was decreased in the Old mice compared to the Young. However, it increased with exercise training in the Old. Lipidation LC3-II, Becline-1, and Atg7 were decreased in the Old mice compared to the Young. However, Lipidation LC3-II was significantly increased in the trained Old mice (Young:1 Vs Old:$1.32{\pm}0.042$, p<0.05). Based on these data, we suggest that autophagy regulatory events are the attenuated in Old mice, but that they are enhanced with exercise training.

• Journal of Life Science
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• v.17 no.7 s.87
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• pp.889-895
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• 2007

A conventional kinesin, KIF5/kinesin-I, is composed of two kinesin heavy chains (KHCs) and two kinesin light chains (KLCs) and binds directly to microtubules. KIF5 motor mediates the transport of various membranous organelles, but the mechanism how they recognize and bind to a specific cargo has not yet been completely elucidated. Here, we used the yeast two-hybrid system to identify the neuronal protein(s) that interacts with the tetratricopeptide repeats (TRP) of KLCI and found a specific interaction with JNK/stress-activated protein kinase-associated protein 1 (JSAP1/JIPP3). The yeast two-hybrid assay demonstrated that the TRP 1,2 domain-containing region of KLCI mediated binding to the leucine zipper domain of JSAP1. JSAP1 also bound to the TRP region of lac2 but not to neuronal KIF5A, KIF5C and ubiquitous KIF5B in the yeast two-hybrid assay. In addition, these proteins showed specific interactions in the GST pull-down assay and by co-immunoprecipitation. KLCI and KIF5B interacted with GST-ISAP1 fusion proteins, but not with GST alone. An antibody to JSAPI specifically co-immunoprecipitated KIF5s associated with JSAP1 from mouse brain extracts. These results suggest that JSAP1, as KLC1 receptor, is involved in the KIF5 mediated transport.

• Journal of Nutrition and Health
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• v.53 no.5
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• pp.518-531
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• 2020

Purpose: This study examined the association between fruit and vegetable intake and mental health in Korean adolescents. Methods: This study used the data from the 2014-2017 Korea Youth Risk Behavior Survey, a national cross-sectional survey on 137,101 boys and 130,806 girls aged 12-18. Fruit and vegetable intake was assessed based on the frequency of consumption. The outcome variables were the perceived happiness, perceived stress, depressive symptom and suicidal ideation over the previous 12 months. Logistic regression models were used after adjusting for the demographic, life style and other dietary factors. Results: Only 34% and 29% of Korean adolescents consumed fruits more than 5 times/week and vegetables more than 2 times/day, respectively; whereas 37%, 25% and 12.2% of Korean adolescents had perceived stress, depressive symptom and suicidal ideation, respectively. After adjusting for the confounding variables, the greater consumption of fruit and vegetable were all associated with a higher odds of perceived happiness; the adjusted odds ratios (AORs) (95% CI) were 1.53 (1.46-1.60) in boys and 1.82 (1.73-1.90) in girls who consumed fruit ≥ 5 times/week, and 1.65 (1.54-1.76) in boys and 1.62 (1.51-1.72) in girls who consumed vegetable ≥ 2 times/day. In contrast, the consumption of fruit or vegetable were all significantly associated with a lower odds of perceived stress, depressive symptom, and suicidal ideation; the AOR (95% CI) were 0.70 (0.67-0.73), 0.88 (0.84-0.93), and 0.78 (0.73-0.83) in boys who consumed fruit 3-4 times/week, and 0.71 (0.67-0.76), 0.88 (0.81-0.94), and 0.68 (0.62-0.74) in boys who consumed vegetable 5-7 times/week. Similar associations of fruit or vegetable consumption with perceived stress, depressive symptom, or suicidal ideation were found in girls. Conclusion: These findings provide evidence that increasing fruit and vegetable intake is important for better mental health among adolescents.

• Journal of Oral Medicine and Pain
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• v.31 no.3
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• pp.199-210
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• 2006

The epidemiological researches on the inpatients hospitalized at the oral medicine ward have been continuously carried out since 1970, and most researches have been performed by centering around the oral medicine wards of college hospitals. Numerous specialists have been produced after the establishment of oral medicine, and they have been active in various fields. As dental clinics have gotten bigger, the function of oral medicine in the secondary clinics is being brought out. As admissive channel, case features, case composition and otherwise have not been researched for a long time, the related researches should be carried out from now on. Hereupon, this study was carried out by targeting the 100 inpatients hospitalized at the oral medicine ward of Sun Hospital located in Daejeon Korea, through questionnaire. As the result, the following results were derived. 1. The ages of the inpatients in Sun Hospital were $29.21{\pm}11.31$ on the average; 71 females' mean average was $29.63{\pm}11.29$ and 29 males' mean average was $28.17{\pm}11.48$. In regard of school career, the patients who finished high-school course or higher accounted for 78%; the patients' school career seemed to be relatively high. The patients who complained of temporomandibular pain accounted for the highest proportion with 65%. In motivation to visit this hospital, internet surfing was 11%, mass media was 10%, acquaintance's introduction was 38%. The patients, who were hospitalized at another hospital due to the same symptom, accounted for 56%. The dental clinics, which made the patients visit this hospital, accounted for 20%. The patients, who were previously aware that the present symptom should be treated by oral medicine, accounted for 38%. The patients, who were not aware of the fact in advance, were 62%. The respondents of 51% answered that they were aware of the fact one month or below before hospitalization. 2. The patients, who complained of craniocervical ache, accounted for 58%; the patients, whose ache aches affect dailylife, were 22%. Continuous ache was 14% and intermittent ache was 68%, and dull pain was 23%. 3. Life variations were compared with each other by using SRRS (Social Readjustment Rating Scale). In consequence, the variation within 3 years indicated a significant difference in the both groups but the variation within 6 months did not indicate any differences. 4. In regard of the questionnaire on the incidents happened for a week, the ache-group was compared with the group free from the ache. As the result, the number of strain arisen for a week, the decrease of favorite works and sudden fear indicated a significant difference. Pleasant feeling and the decrease of interests in looks did not indicate a significant difference, but came close to the significance. 5. In the questionnaire on impatience, the ache-group indicated higher value but there was not a significant difference. 6. In the questionnaire on the symptoms caused by stress, the two groups indicated significant differences in the item of 'the teethridge itches and feels a tooth rising' and 'the occiput or the nape is stiff.' In the item 'the inside of the cheek or the teethridge are widely peeled off, accompanied with ache and hemorrhage', 'the face has acne or pimple' and 'headache frequently attacks', a significant difference was not observed but the two groups came close to the significance.

• Journal of Life Science
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• v.27 no.8
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• pp.945-950
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• 2017

To some extent, the growth of solvent-tolerant Pseudomonas sp. BCNU 106 is limited by toxic solvents. Therefore, various strategies to overcome this limitation need to be investigated. One such strategy is to use exogenous trehalose. The highest intracellular trehalose content of 181.88 mM was measured at 12 hr. The extracellular trehalose content decreased rapidly within 12 to 16 hr in the presence of cyclohexane. Moreover, the number of Pseudomonas sp. BCNU 106 cells grown in Luria-Bertani (LB) broth supplemented with 0.1 M trehalose in the presence of 1%(v/v) cyclohexane, hexane, propylbenzene, and m-xylene increased 89.94-, 89.72-, 91.25-, and 118.9-fold, respectively, in comparison to the control level. High survival rates of 80% and 90% were observed in the presence of cyclohexane and hexane by the addition of 0.05 M trehalose for up to 4 hr, respectively. Exogenously-added trehalose was transported into the cells, and it conferred protection against cyclohexane, hexane, propylbenzene, and m-xylene. Adding exogenous trehalose to the growth medium improved the tolerance of Pseudomonas sp. BCNU 106; thus, it is a potential biocatalyst for biotransformation and biodegradation.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.37 no.3
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• pp.429-436
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• 2013

This study considers how the fatigue behavior and probabilistic properties of SAE1055 steel are related to its hardness level. SAE1055 steel was heat-treated using induction hardening. Five types of specimens were prepared (A: base material, B: through hardened material with HV390, C: through hardened material with HV510, D: through hardened material with HV700, and E: surface hardened material with HV700). Fatigue tests were performed under a stress ratio of R = -1 using a 4-point rotary bending fatigue tester. The fatigue behaviors were greatly influenced by the hardness, but the fatigue limit did not increase over a hardness of HV510. In addition, the effect of the hardness level on the failure mechanism was evaluated using a scanning electron microscope. The probabilistic properties of the fatigue life were investigated using a probabilistic S-N approach, and the effect of the hardness level on these properties was evaluated using a residue analysis.

• YAKHAK HOEJI
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• v.54 no.1
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• pp.55-61
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• 2010

Gardenia jasminoides is one of the most widely used herbal preparations for the treatment of liver disorders. This study evaluated the potential beneficial effect of G. jasminoides in a mouse model of carbon tetrachloride ($CCl_4$)-induced liver injury. The mice were treated intraperitoneally with $CCl_4$ (10 ${\mu}l$/kg). They received G. jasminoides (30, 100, 300 mg/kg) 48 h, 24 h and 2 h before and 6 h after administering $CCl_4$. The serum activities of aminotransferase and the hepatic level of malondialdehyde were significantly higher 24 h after the $CCl_4$ treatment, while the concentration of reduced glutathione was lower. These changes were attenuated by G. jasminoides. $CCl_4$ increased the level of circulating tumor necrosis factor-$\alpha$ (TNF-$\alpha$) markedly, which was reduced by G. jasminoides. The levels of hepatic inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein expression were markedly higher after the $CCl_4$ treatment. G. jasminoides diminished these alterations. $CCl_4$ increased the level of TNF-$\alpha$, iNOS and COX-2 mRNA expressions, and these increases were attenuated by G. jasminoides. These results suggest that G. jasminoides alleviates $CCl_4$-induced liver injury, and this protection is likely due to the reduced oxidative stress and the downregulation of proinflammatory mediators.

• Journal of the Korea Convergence Society
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• v.12 no.4
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• pp.359-367
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• 2021

The purpose of this study is conceptual analysis study on the thwarted belongingness, and was based on the conceptual analysis method of Walker & Avant to make clear concept. Thwarted belongingness can be defined by the following attributes: distorted beliefs, insufficient need to belong, a sense of alienation, and insufficient interaction and affection needs. The prerequisites for thwarted belongingness were social isolation, experiences of exclusion or conflict in interpersonal relationships, perfectionist tendencies, and sensitivity to rejection. The consequences occurring as a result of thwarted belongingness were suicidal ideation, decreased happiness and life satisfaction, negative emotions such as anxiety about rejection, depression, shame, loneliness, stress or anger, aggression, hostility and self-criticism, despair, suppression of emotional expression, lethargy, self-exclusion from relationships, and identity confusion. This study is valuable to identify the extent of preventable nursing interventions related to individual mental health and suicidal ideation related to interpersonal relationships, and suggests future tool development and field studies of thwarted belongingness.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.17 no.5
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• pp.1377-1393
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• 2023

Social media is used for various purposes including entertainment, communication, information search, and voicing their thoughts and concerns about a service, product, or issue. The social media data can be used for information mining and getting insights from it. The World Health Organization has listed COVID-19 as a global epidemic since 2020. People from every aspect of life as well as the entire health system have been severely impacted by this pandemic. Even now, after almost three years of the pandemic declaration, the fear caused by the COVID-19 virus leading to higher depression, stress, and anxiety levels has not been fully overcome. This has also triggered numerous kinds of discussions covering various aspects of the pandemic on the social media platforms. Among these aspects is the part focused on vaccines developed by different countries, their features and the advantages and disadvantages associated with each vaccine. Social media users often share their thoughts about vaccinations and vaccines. This data can be used to determine the popularity levels of vaccines, which can provide the producers with some insight for future decision making about their product. In this article, we used Twitter data for the vaccine popularity detection. We gathered data by scraping tweets about various vaccines from different countries. After that, various machine learning and deep learning models, i.e., naive bayes, decision tree, support vector machines, k-nearest neighbor, and deep neural network are used for sentiment analysis to determine the popularity of each vaccine. The results of experiments show that the proposed deep neural network model outperforms the other models by achieving 97.87% accuracy.

• Journal of the Korean Institute of Landscape Architecture
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• v.39 no.5
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• pp.111-118
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• 2011

With increasing interest in health promotion and quality of life, growing attention has been focused on the beneficial effects of urban green area. However, very few evidence-based approaches have been conducted on the health-related benefits of urban greenery. Therefore, this study examined the health-related benefits of green areas using physiological and psychological indices to obtain evidence-based data on these benefits. Twenty male university students were selected as subjects. Data were collected when participants viewed landscapes in a green area or an urban area for fifteen minutes. This research was reviewed and approved by the Ethics Committee of School of Medicine, Chungnam National University. Physiological data in the green area revealed significantly decreased heart rates, significantly increased high-frequency value of heart rate variability, an index of parasympathetic activity, and reduced salivary cortisol concentration, a stress hormone, compared to the urban area. Psychological tests showed the green area significantly reduced the negative mood state and psychological symptoms, and significantly increased the positive mood state. Our data provided evidence for the health-related benefits of green areas, and the findings of this study support that green areas can play a critical role in health promotion for urban residents, by positively affecting autonomic nervous and endocrinal activities.