• Title/Summary/Keyword: Stress Parameters

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Changes in Productivity and Morphological Characteristics of Zostera marina Transplants (이식된 잘피의 생산성 및 형태적 특성 변화)

  • Park, Jung-Im;Li, Wentao;Kim, Jeong-Bae;Lee, Kun-Seop
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.14 no.1
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    • pp.41-47
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    • 2009
  • Since significant losses of seagrass coverage have been reported from many parts of the world, numerous restoration projects through seagrass transplantation have been attempted worldwide. Different survival rates and establishment time of transplants have been reported depending on transplanting time and methods. The staple method, which is direct seagrass planting method using staples to anchor seagrass transplants on the sediments, have been widely adopted in seagrass transplanting because this method achieves high survival rates in various sediment environments. To assess the morphological plasticity and the growth characteristics of transplants, we transplanted eelgrass, Zostera marina in December 2004 using the staple method. Shoot density, morphological characteristics and leaf productivities of the transplanted shoots and shoots of natural eelgrass beds in the vicinity of the transplanting site and environmental parameters in the planting site were monitored for about 1 year postplanting monthly. Transplant shoot density increased without initial decline, while leaf width and sheath length of transplants decreased after transplanting. Leaf productivities per shoot of transplants also considerably lower than those of natural shoots for the first 3 months post-transplanting. Shoot density, morphological characteristics and leaf productivity per area of transplants became similar to those of natural population about 1 year after transplanting. Although eelgrass transplants might have experienced some transplanting stress during the early stage of the transplantation, transplants appeared to adapt well to new environments of the transplanting site.

Blood Property and Biologger Attachment Efficiency of Spotted Sea bass Lateolabrax maculatus depending on External Biologger Attachment Methods (바이오로거 체외 부착방법이 점농어(Lateolabrax maculatus)의 혈액성상 및 바이오로거 부착효율에 미치는 영향)

  • Pil Jun Kang;Geun Su Lee;Sung-Yong Oh
    • Journal of Marine Life Science
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    • v.9 no.1
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    • pp.22-32
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    • 2024
  • The purpose of this study was to determine the effect of external biologger attachment methods on the blood parameters and attachment efficiency of spotted sea bass Lateolabrax maculatus (mean body weight 2630.8 g). The fish were tagged using four different external attachment methods with dummy biologgers: no attachment (control), anchor attachment (AA), monofilament attachment (MA), and silicon tube attachment (SA), each with triplicates. Blood indices and biologger attachment efficiency were assessed on days 1, 7, 14, 28, 56, and 84 after attachment. The concentrations of hematocrit, Na+, Cl-, glutamic pyruvic transaminase and total protein, and the activity of superoxide dismutase in blood were not affected by the external attachment method of biologger. The concentrations of glutamic oxaloacetic transaminase (day 1 of attachment), hemoglobin (day 56) and total cholesterol (day 56 and 84) in AA group, the concentrations of glucose and cortisol (day 14) and total cholesterol (day 84) in MA group showed significantly higher than those of control (p<0.05). During the experiment period, the SA group had no differences from the control in all blood properties. The biologger attachment efficiencies of the AA, MA, and SA groups after 84 days were 0.0%, 33.3%, and 100.0%, respectively. These results indicate that the optimum external biologger attachment method under our experimental conditions is SA type.

Rutin alleviated lipopolysaccharide-induced damage in goat rumen epithelial cells

  • Jinshun Zhan;Zhiyong Gu;Haibo Wang;Yuhang Liu;Yanping Wu;Junhong Huo
    • Animal Bioscience
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    • v.37 no.2
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    • pp.303-314
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    • 2024
  • Objective: Rutin, also called vitamin P, is a flavonoids from plants. Previous studies have indicated that rutin can alleviate the injury of tissues and cells by inhibiting oxidative stress and ameliorating inflammation. There is no report on the protective effects of rutin on goat rumen epithelial cells (GRECs) at present. Hence, we investigated whether rutin can alleviate lipopolysaccharide (LPS)-induced damage in GRECs. Methods: GRECs were cultured in basal medium or basal medium containing 1 ㎍/mL LPS, or 1 ㎍/mL LPS and 20 ㎍/mL rutin. Six replicates were performed for each group. After 3-h culture, the GRECs were harvested to detect the relevant parameters. Results: Rutin significantly enhanced the cell activity (p<0.05) and transepithelial electrical resistance (TEER) (p<0.01) and significantly reduced the apoptosis rate (p<0.05) of LPS-induced GRECs. Rutin significantly increased superoxide dismutase, glutathione peroxidase, and catalase activity (p<0.01) and significantly decreased lactate dehydrogenase activity and reactive oxygen species and malondialdehyde (MDA) levels in LPS-induced GRECs (p<0.01). The mRNA and protein levels of interleukin 6 (IL-6), IL-1β, and C-X-C motif chemokine ligand 8 (CXCL8) and the mRNA level of tumor necrosis factor-α (TNF-α) and chemokine C-C motif ligand 5 (CCL5) were significantly increased in LPS-induced GRECs (p<0.05 or p<0.01), while rutin supplementation significantly decreased the mRNA and protein levels of IL-6, TNF-α, and CXCL8 in LPS-induced GRECs (p<0.05 or p<0.01). The mRNA level of toll-like receptor 2 (TLR2), and the mRNA and protein levels of TLR4 and nuclear factor κB (NF-κB) was significantly improved in LPS-induced GRECs (p<0.05 or p<0.01), whereas rutin supplementation could significantly reduce the mRNA and protein levels of TLR4 (p<0.05 or p<0.01). In addition, rutin had a tendency of decreasing the protein levels of CXCL6, NF-κB, and inhibitor of nuclear factor kappa-B alpha (0.05

Case Study on the Application and Evaluation of an Integrated Medical Service Model to Improve the Quality of Life for Breast Cancer Patients and Caregivers (유방암 환자와 보호자의 삶의 질 증진을 위한 통합의료서비스모델 적용평가 사례 연구)

  • Moon Joo Cheong;Do-Eun Lee;Un Jong Choi;Han Baek Cho;Hyung Won Kang
    • Journal of The Korean Society of Integrative Medicine
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    • v.12 no.3
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    • pp.163-178
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    • 2024
  • Purpose : This study aimed to evaluate the effectiveness of an integrative medical service model applied to breast cancer patients and their caregivers, exploring its feasibility and challenges within the context of South Korean healthcare system. Methods : A case study approach was chosen to assess the integrative medical service model's efficacy, involving one breast cancer patient and her primary caregiver from W University Hospital. The patient had completed reconstructive surgery and chemotherapy and was undergoing radiotherapy. The model included standard treatments alongside psychological counseling, aromatherapy, axillary rehabilitation exercise, make-up program, art therapy, laughter therapy, horticultural therapy, and yoga programs, and meditation programs delivered over eight weeks. Quantitative and qualitative data were collected through surveys, psychological tests, and feedback assessments. Results : The integrative medical service model demonstrated notable improvements in the quality of life for both breast cancer patients and their caregivers. Participants reported enhanced emotional well-being, reduced stress levels, and improved coping mechanisms throughout the treatment journey. Qualitative feedback highlighted the positive impact of holistic interventions in alleviating psychological distress and fostering resilience. Quantitative data corroborated these findings, showing statistically significant improvements in various psychosocial parameters assessed. Conclusions : Our findings underscore the benefits of integrative medical service model with standard medical treatments in the care of breast cancer patients and their caregivers. The holistic approach not only addresses physical symptoms but also enhances overall well-being and quality of life. However, the implementation of such models faces challenges within the South Korean healthcare system, including fragmented service networks and financial constraints. Addressing these structural barriers is crucial for the widespread adoption and sustainability of integrative care models in oncology practice. Future research should focus on larger-scale studies to further validate these findings and inform policy decisions aimed at optimizing cancer care delivery.

Alteration of Biosynthesis and Secretion of Adrenal Catecholamines in Cycling Rat (발정주기 중 흰쥐 부신에서의 카테콜아민 합성과 분비 변화)

  • 이성호
    • Development and Reproduction
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    • v.6 no.2
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    • pp.105-110
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    • 2002
  • Numerous hormones are involved in the regulation of reproduction. Among them, estrogen and progesterone are the most important ovarian steroid hormones regulating female fertility. On the other hand, diverse stressors impede female receptivity and fertility. Since norepinephrine(NE) and epinephrine(E) are released from the adrenal during stress, it might play a role in stress-induced disruptions of fEmale reproductive parameters. The present study was performed to analyze the changes in adrenal catecholaminergic activities in cycling rats. The tissue content and secretion level of catecholamines were determined by high performance liquid chromatography coupled with electrochemical detector(HPLC-ECD). Adrenomedullary content of norepinephrine(NE) was increased on proestrus stage (59.47 $\pm$ 6.86 ug/gland), peaked on diestrus I stage(65.22 $\pm$ 5.99 ug/gland), and was nadir on diestrus II stage(41.63 $\pm$ 1.33 ug/gland). The highest E content was observed on proestrus stage(361.86 $\pm$ 15.58 ug/gland) while the lowest level was on diestrus II stage(285.58 $\pm$ 12.25 ug/gland). In addition to these observations, a significant reduction of the NE : E ratio was observed (1 : 4.81 on diestrus I vs 1 : 6.13~7.02 on other stages). In vitro secretion of adrenal NE and E was increased on proestrus stage, peaked on estrus stage, and decreased on diestrus II stage. Interestingly, the NE : E ratio in conditioned media was significantly increased on estrus stage (1 : 3.32 vs 1 : 2.34~2.65 on other stages. The biosynthesis of NE and E is mediated by tyrosine hydroxylase(TH) and phenylethanolamine-N-methyltransferase(PNMT) which acts conversion of tyrosine into DOPA and NE into E, respectively. These finding demonstrated that sex steroids, during setrous cycle, seem to be able to modify the adrenal catecholamines biosynthesis and secretion with stage-specific manner by modulation of the enzyme activities.

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Posterior Cruciate Ligament Augmentation Using an Autogenous Hamstring Tendon Graft and the Posterior-Posterior Triangulation Technique (만성 단독 후방십자인대 파열에서 자가 슬괵건과 후-후 삼각술기를 이용한 만성 후방십자인대 보강술)

  • Kim, Yeung Jin;Chae, Soo Uk;Choi, Byong San;Kim, Jong Yun;Lee, Hwang Yong;Han, Chang Wan;Han, Su Hyoun
    • Journal of the Korean Arthroscopy Society
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    • v.17 no.1
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    • pp.24-30
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    • 2013
  • Purpose: To evaluate the outcome of arthroscopic posterior cruciate ligament (PCL) augmentation using an autogenous hamstring tendon graft and the posterior-posterior triangulation technique with preservation of ligament remnant or elongated ligament. Materials and Methods: From January 2002 to December 2009, we performed PCL augmentation using an autogenous hamstring tendon graft and the posterior-posterior triangulation technique in 32 patients. The mean age was 35.2 years. Twenty two cases were male and 10 cases were female. Average follow-up period was 5 years and 5 months (range: 2-7.9). Subjective and objective parameters were utilized in analyses, such as the mean range of motion, post. drawer test, Lysholm knee score, Tegner activity score, International Knee Documentation Cominittee (IKDC) grade, and second look arthroscopic examination. Results: At last follow up posterior displacement by the Telos stress test decreased from $10.8{\pm}5.1\;mm$ to $2.8{\pm}3.7\;mm$ (p<0.05). The final Lysholm knee score improved from $60.4{\pm}5.8$ to $84.6{\pm}4.8$. Tegner activity score improved from 3.2 to 4.8. The final IKDC grade was A in 18, B in 11, C in 3. Postoperative Lysholm knee score, IKDC grades, Tegner activity scale, and posterior displacement demonstrated statistically significant improvement compared to the preoperative state (p<0.05). Conclusion: Arthroscopic PCL reconstruction using an autogenous hamstring tendon with preservation of ligament remnant showed a good clinical results and posterior stability.

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Influence of Column Aspect Ratio on the Hysteretic Behavior of Slab-Column Connection (슬래브-기둥 접합부의 이력거동에 대한 기둥 형상비의 영향)

  • Choi, Myung-Shin;Cho, In-Jung;Ahn, Jong-Mun;Shin, Sung-Woo
    • Journal of the Korea Concrete Institute
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    • v.19 no.4
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    • pp.515-525
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    • 2007
  • In this investigation, results of laboratory tests on four reinforced concrete flat plate interior connections with elongated rectangular column support which has been used widely in tall residential buildings are presented. The purpose of this study is to evaluate an effect of column aspect ratio (${\beta}_c={c_1}/{c_2}$=side length ratio of column section in the direction of lateral loading $(c_1)$ to the direction of perpendicular to $c_1$) on the hysteretic behavior under earthquake type loading. The aspect ratio of column section was taken as $0.5{\sim}3\;(c_1/c_2=1/2,\;1/1,\;2/1,\;3/1)$ and the column perimeter was held constant at 1200mm in order to achieve nominal vertical shear strength $(V_c)$ uniformly. Other design parameters such as flexural reinforcement ratio $(\rho)$ of the slab and concrete strength$(f_{ck})$ was kept constant as ${\rho}=1.0%$ and $f_{ck}=40MPa$, respectively. Gravity shear load $(V_g)$ was applied by 30 percent of nominal vertical shear strength $(0.3V_o)$ of the specimen. Experimental observations on punching failure pattern, peak lateral-load and story drift ratio at punching failure, stiffness degradation and energy dissipation in the hysteresis loop, and steel and concrete strain distributions near the column support were examined and discussed in accordance with different column aspect ratio. Eccentric shear stress model of ACI 318-05 was evaluated with experimental results. A fraction of transferring moment by shear and flexure in the design code was analyzed based on the test results.

STRAIN AND TEMPERATURE CHANGES DURING THE POLYMERIZATION OF AUTOPOLYMERIZING ACRYLIC RESINS

  • Ahn Hyung-Jun;Kim Chang-Whe;Kim Yung-Soo
    • The Journal of Korean Academy of Prosthodontics
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    • v.39 no.6
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    • pp.709-734
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    • 2001
  • The aims of this experiment were to investigate the strain and temperature changes simultaneously within autopolymerzing acrylic resin specimens. A computerized data acquisition system with an electrical resistance strain gauge and a thermocouple was used over time periods up to 180 minutes. The overall strain kinetics, the effects of stress relaxation and additional heat supply during the polymerization were evaluated. Stone mold replicas with an inner butt-joint rectangular cavity ($40.0{\times}25.0mm$, 5.0mm in depth) were duplicated from a brass master mold. A strain gauge (AE-11-S50N-120-EC, CAS Inc., Korea) and a thermocouple were installed within the cavity, which had been connected to a personal computer and a precision signal conditioning amplifier (DA1600 Dynamic Strain Amplifier, CAS Inc., Korea) so that real-time recordings of both polymerization-induced strain and temperature changes were performed. After each of fresh resin mixture was poured into the mold replica, data recording was done up to 180 minutes with three-second interval. Each of two poly(methyl methacrylate) products (Duralay, Vertex) and a vinyl ethyl methacrylate product (Snap) was examined repeatedly ten times. Additionally, removal procedures were done after 15, 30 and 60 minutes from the start of mixing to evaluate the effect of stress relaxation after deflasking. Six specimens for each of nine conditions were examined. After removal from the mold, the specimen continued bench-curing up to 180 minutes. Using a waterbath (Hanau Junior Curing Unit, Model No.76-0, Teledyne Hanau, New York, U.S.A.) with its temperature control maintained at $50^{\circ}C$, heat-soaking procedures with two different durations (15 and 45 minutes) were done to evaluate the effect of additional heat supply on the strain and temperature changes within the specimen during the polymerization. Five specimens for each of six conditions were examined. Within the parameters of this study the following results were drawn: 1. The mean shrinkage strains reached $-3095{\mu}{\epsilon},\;-1796{\mu}{\epsilon}$ and $-2959{\mu}{\epsilon}$ for Duralay, Snap and Vertex, respectively. The mean maximum temperature rise reached $56.7^{\circ}C,\;41.3^{\circ}C$ and $56.1^{\circ}C$ for Duralay, Snap, and Vertex, respectively. A vinyl ethyl methacrylate product (Snap) showed significantly less polymerization shrinkage strain (p<0.01) and significantly lower maximum temperature rise (p<0.01) than the other two poly(methyl methacrylate) products (Duralay, Vertex). 2. Mean maximum shrinkage rate for each resin was calculated to $-31.8{\mu}{\epsilon}/sec,\;-15.9{\mu}{\epsilon}/sec$ and $-31.8{\mu}{\epsilon}/sec$ for Duralay, Snap and Vertex, respectively. Snap showed significantly lower maximum shrinkage rate than Duralay and Vertex (p<0.01). 3. From the second experiment, some expansion was observed immediately after removal of specimen from the mold, and the amount of expansion increased as the removal time was delayed. For each removal time, Snap showed significantly less strain changes than the other two poly(methyl methacrylate) products (p<0.05). 4. During the external heat supply for the resins, higher maximum temperature rises were found. Meanwhile, the maximum shrinkage rates were not different from those of room temperature polymerizations. 5. From the third experiment, the external heat supply for the resins during polymerization could temporarily decrease or even reverse shrinkage strains of each material. But, shrinkage re-occurred in the linear nature after completion of heat supply. 6. Linear thermal expansion coefficients obtained from the end of heat supply continuing for an additional 5 minutes, showed that Snap exhibited significantly lower values than the other two poly(methyl methacrylate) products (p<0.01). Moreover, little difference was found between the mean linear thermal expansion coefficients obtained from two different heating durations (p>0.05).

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Influence of Short-term Application of Abscisic Acid in Nutrient Solution on Growth and Drought Tolerance of Tomato Seedlings (토마토 육묘과정에서 단기간 ABA처리가 묘소질과 건조내성에 미치는 영향)

  • Kim, Il-Seop;Vu, Ngoc-Thang;Vo, Hoang-Tung;Choi, Ki-Young;Kim, Young Shik
    • Journal of Bio-Environment Control
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    • v.24 no.1
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    • pp.13-20
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    • 2015
  • This study was conducted to evaluate influence of short-term application of abscisic acid (ABA) in nutrient solution on growth and drought tolerance of tomato seedlings. The treatments included four ABA concentrations (0.5, 1, 2, $3mg{\cdot}L^{-1}$) and control (non-treatment) were applied to the nutrient solution in a hydroponic system. On the $5^{th}$ and $10^{th}$ day after growing in the nutrient solution containing ABA, seedlings were transferred to -5 bars of PEG-8000 in a growth chamber to induce water stress. Except for stem diameter and fresh and dry weight of root, there were no statistical differences in other growth parameters among control, 0.5 and $1mg{\cdot}L^{-1}$ of ABA treatments. Seedlings growths were strongly inhibited in nutrient solution containing 2 and $3mg{\cdot}L^{-1}$ of ABA. The root growth such as fresh and dry weigh of root, total root surface area, and average root diameter was slightly enhanced in $1mg{\cdot}L^{-1}$ of ABA treatment. The elevation of ABA concentrations in nutrient solution resulted in the decrease in transpiration rate and increase in stomatal diffusive resistance and leaf temperature of tomato seedlings. The initiations of seedling wilting after treating in -5 bars of PEG were delayed from 10 hrs in control to 30 hrs in ABA applied treatments. Additionally, the high percentages of recovered seedlings were observed in 0.5 and $1mg{\cdot}L^{-1}$ of ABA treatments after re-irrigation. Therefore, short-term application of $1mg{\cdot}L^{-1}$ of ABA in the nutrient solution stimulated the root growth and drought tolerance of tomato seedlings by delaying the start time of wilting point and enhancing the recovery after re-irrigation.

Amended Soil with Biopolymer Positively Affects the Growth of Camelina sativa L. Under Drought Stress (가뭄 조건 하에서 바이오폴리머 혼합 토양이 Camelina sativa L.의 생장에 미치는 긍정적 영향)

  • Lim, Hyun-Gyu;Kim, Hyun-Sung;Lee, Hyeon-Sook;Sin, Jung-Ho;Kim, Eun-Suk;Woo, Hyo-Seop;Ahn, Sung-Ju
    • Ecology and Resilient Infrastructure
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    • v.5 no.3
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    • pp.163-173
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    • 2018
  • The biopolymer (BP) used in this study is mainly composed of xanthan gum and ${\beta}$-glucan derived from microorganism and has been introduced as a novel material for soil stabilization. However, the broad applicability of BP has been suggested in the field of geotechnical engineering while little information is available about the effects of BP on the vegetation. The goal of this study is to find the BP effects on the growth of Camelina sativa L. (Camelina) under drought condition. For more thorough evaluation of BP effects on the plant growth, we examined not only morphological but also physiological traits and gene expression patterns. After 25 days of drought treatment from germination in the soil amended with 0, 0.25, 0.5, and 1% BP, we observed that the BP concentration was strongly correlated the growth of Camelina. When plants were grown under drought stress, Camelina in 0.5% BP mixture showed better physiological parameters of the leaf stomatal conductance, electrolyte leakage and relative water content compared to those in control soil without BP. Plant recovery rate after re-watering was higher and the development of lateral root was lower in BP amended soil. RNA expression of Camelina leaf treated with/without drought for 7 and 10 days showed that aquaporin genes transporting solutes at bio-membrane, CsPIP1;4, 2;1, 2;6 and TIP1;2, 2;1, were induced more in the plants with BP amendment and drought treatment. These results suggest that the soil amended with BP has a positive effect on the transport of nutrients and waters into Camelina by improving water retention in soil under drought condition.