• Molecules and Cells
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• 제25권3호
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• pp.368-375
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• 2008

Approximately 120 UDP-glycosyltransferases (UGTs), which are classified into 14 distinct groups (A to N), have been annotated in the Arabidopsis genome. UGTs catalyze the transfer of sugars to various acceptor molecules including flavonoids. Previously, UGT71C1 was shown to glycosylate the 3-OH of hydroxycinnamates and flavonoids in vitro. Such secondary metabolites are known to play important roles in plant growth and development. To help define the role of UGT71C1 in planta, we investigated its expression patterns, and isolated and characterized a loss-of-function mutation in the UGT71C1 gene (named ugt71c1-1). Our analyses by quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR), microarray data mining, and histochemical detection of GUS activity driven by the UGT71C1 promoter region, revealed the tissue-specific expression patterns of UGT71C1 with highest expression in roots. Interestingly, upon treatment with methyl viologen (MV, paraquat), ugt71c1-1 plants displayed enhanced resistance to oxidative stress, and ROS scavenging activity was higher than normal. Metabolite profiling revealed that the levels of two major glycosides of quercetin and kaempferol were reduced in ugt71c1-1 plants. In addition, when exposed to MV-induced oxidative stress, eight representative ROS response genes were expressed at lower levels in ugt71c1-1 plants, indicating that ugt71c1-1 probably has higher non-enzymatic antioxidant activity. Taken together, our results indicate that ugt71c1-1 has increased resistance to oxidative stress, suggesting that UGT71C1 plays a role in some glycosylation pathways affecting secondary metabolites such as flavonoids in response to oxidative stress.

• 한국재료학회지
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• 제33권4호
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• pp.124-129
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• 2023

This study assessed the influences of fluorine introduced into DLC films on the structural and mechanical properties of the sample. In addition, the effects of the fluorine incorporation on the compressive stress in DLC films were investigated. For this purpose, fluorinated diamond-like carbon (F-DLC) films were deposited on cobalt-chromium-molybdenum substrates using radio-frequency plasma-enhanced chemical vapor. The coatings were examined by Raman scattering (RS), Attenuated total reflectance Fourier transform infrared spectroscopic analysis (ATR-FTIR), and a combination of elastic recoil detection analysis and Rutherford backscattering (ERDA-RBS). Nano-indentation tests were performed to measure hardness. Also, the residual stress of the films was calculated by the Stony equation. The ATR-FTIR analysis revealed that F was present in the amorphous matrix mainly as C-F and C-F₂ groups. Based on Raman spectroscopy results, it was determined that F made the DLC films more graphitic. Additionally, it was shown that adding F into the DLC coating resulted in weaker mechanical properties and the F-DLC coating exhibited lower stress than DLC films. These effects were attributed to the replacement of strong C = C by feebler C-F bonds in the F-DLC films. F-doping decreased the hardness of the DLC from 11.5 to 8.8 GPa. In addition, with F addition, the compressive stress of the DLC sample decreased from 1 to 0.7 GPa.

• 한국미생물·생명공학회지
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• 제27권4호
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• pp.278-285
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• 1999

In order to use heat shock promoter for the detection of toxic substances, dnaK promoter was amplified from E. coli genomic DNA by using a polymerase chain reaction(PCR) followed by sequencing and sub-cloning into the multi-cloning site of the plasmid, pUCD615. The pUCD615 is a broad-host-range vector containing promoterless lux operon originated from V.fischeri. The recombinant plasmid was transfered to E. coli DH5$\alpha$ through electroporation. The recombinant E. coli showed several patterns of bioluminescent responses to ethanol stress. The bioluminescent E. coli also showed responses to other toxic substances including FeK3(CN)6, CdCl2, p-nitrophenol and HgCl2. The increases of RLU(Relative Light Unit) were observed at 100ppm of FeK3(CN)6, 10ppm and 100ppm and 100ppm of CdCl2, 1ppm of 10ppm of p-nitrophenol and at 1ppm of HgCl2.

• Asian Pacific Journal of Cancer Prevention
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• 제16권1호
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• pp.31-34
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• 2015

While mammography has been used for diagnosis of breast cancer in Kazakhstan for a long period, published data are very limited. Recently stress has been placed on increasing the accuracy by double reading of mammograms. Here we provide an overview of breast cancer screening in the different regions of Kazakhstan with data on the stages of cancers detected. A total 459,816 women aged 50, 52, 54, 56, 58 and 60 years were screened in 2012 and 379,903 in the first 9 months of 2013. Clear differences in levels of detection were noted between urban and rural residents, the latter demonstrating lower rates for both screening and cancer detection. Women aged 50 were more likely to undergo screening than their counterparts aged 60. While there were no clear relationships evident between screening rates and stage or numbers of breast cancers observed, this might be due to a number of complicating factors like geographical variation in risk factors as well as ethnicity. Future analyses should focus on the efficacy of mammography in Kazakhstan to reduce mortality.

• Clinical and Experimental Pediatrics
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• 제56권2호
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• pp.45-51
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• 2013

Because nonalcoholic steatohepatitis can progress towards cirrhosis even in children, early detection of hepatic fibrosis and accurate diagnosis of nonalcoholic fatty liver disease (NAFLD) are important. Although liver biopsy is regarded as the gold standard of diagnosis, its clinical application is somewhat limited in children due to its invasiveness. Noninvasive diagnostic methods, including imaging studies, biomarkers of inflammation, oxidative stress, hepatic apoptosis, hepatic fibrosis, and noninvasive hepatic fibrosis scores have recently been developed for diagnosing the spectrum of NAFLD, particularly the severity of hepatic fibrosis. Although data and validation are still lacking for these noninvasive modalities in the pediatric population, these methods may be applicable for pediatric NAFLD. Therefore, noninvasive imaging studies, biomarkers, and hepatic fibrosis scoring systems may be useful in the detection of hepatic steatosis and the prediction of hepatic fibrosis, even in children with NAFLD.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2001년도 춘계학술대회논문집A
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• pp.698-703
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• 2001

This paper is concerned with the nonlinear hyperelastic problem fur the incompressible characteristics of the rubber. Tension sensor is a strain gage type load cell element for a fence intrusion detection system and consists of the sensing part and the rubber housing. The analysis includes an elastic analysis and a hyperelastic analysis of a tension sensor for the deformed shape and variation of the maximum strain on the sensing part with respect to the vertical load. Numerical results show that the hyperelastic model is stiffer and less deformed than the elastic model. Comparing with the experimental test data, we know the hyperelastic model is the better approximation than the elastic model.

• 대한전기학회논문지:전기물성ㆍ응용부문C
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• 제55권1호
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• pp.11-15
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• 2006

We propose an optical and an electrical detection methods for detecting various bio-molecules effectively with microcantilevers. The microcantilevers were fabricated employing surface micromachining technique that has attractive advantages in terms of cost efficiency, simplicity and ability of fabricating in array. The fluid cell system for injection of bio-molecular solution is fabricated using polydimethylsiloxane (PDMS) and a fused silica glass. The microcantilever is deflected with respect to the difference of the surface stress caused by the formation of self-assembled bio-molecules on the gold coated side of the microcantilever. It detected cystamine dihydrochloride and glutaraldehyde molecules and analyzed individual concentrations of the cystamine dihydrochloride solution. We confirm that the deflections of bending-up or bending-down are occurred by the bio-molecule adsorption and microcantilever can be widely used to a ${\mu}-TAS$ and a lab-on-a-chip for a potential detection of various bio-molecules.

• 풍력에너지저널
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• 제9권4호
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• pp.16-23
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• 2018

A damage detection method for the tripod support structure of offshore wind turbines is presented for structural health monitoring. A finite element model of a prototype tripod support structure is established and the modal properties are calculated. The degree and location of the damage are estimated based on the neural network technique using the changes of natural frequencies and mode shape due to the damage. The stress distribution occurring in the support structure is obtained by a dynamic analysis for the wind turbine system to select the output data of the neural network. The natural frequencies and mode shapes for 36 possible damage scenarios were used for the input data of the learned neural network for damage assessment. The estimated damages agreed reasonably well with the accurate ones. The presented method could be effectively applied for damage detection and structural health monitoring of various types of support structures of offshore wind turbines.

• 한국항행학회논문지
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• 제27권4호
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• pp.391-395
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• 2023

Helicopter accidents due to spatial disorientation in low visibility conditions continue to persist as a major issue. These incidents often stem from human error, typically induced by stress, and frequently result in fatal outcomes. This study employs machine learning to analyze flight data and evaluate the efficacy of a flight illusion detection algorithm, laying groundwork for further research. This study collected flight data from approximately 20 pilots using a simulated flight training device to construct a range of flight scenarios. These scenarios included three stages of flight: ascending, level, and descent, and were further categorized into good visibility conditions and 0-mile visibility conditions. The aim was to investigate the occurrence of flight illusions under these conditions. From the extracted data, we obtained a total of 54,000 time-series data points, sampled five times per second. These were then analyzed using a machine learning approach.

• EDISON SW 활용 경진대회 논문집
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• 제5회(2016년)
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• pp.195-202
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• 2016

In this paper, crack detection method using eigen value analysis is presented. Three methods are used: theoretical analysis, finite element method with the cracked beam elements and finite element method with three dimensional continuum elements. Finite element formulation of the cracked beam element is introduced. Additional term about stress intensity factor based on fracture mechanics theory is added to flexibility matrix of original beam to model the crack. As using calculated stiffness matrix of cracked beam element and mass matrix, natural frequencies are calculated by eigen value analysis. In the case of using continuum elements, the natural frequencies could be calculated by using EDISON CASAD solver. Several cases of crack are simulated to obtain natural frequencies corresponding the crack. The surface of natural frequency is plotted as changing with crack location and depth. Inverse analysis method is used to find crack location and depth from the natural frequencies of experimental data, which are referred by another papers. Predicted results are similar with the true crack location and depth.