• Microbiology and Biotechnology Letters
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• v.36 no.2
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• pp.106-114
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• 2008

One bacterium, which showed strong antagonistic activity against H. pylori KCCM 41756, was isolated from kimchi. The strain NO1 was designated as Lactobacillus plantarum NO1 based on Gram staining, biochemical properties, and 16S rRNA gene sequencing. The culture medium $(2{\sim}4{\mu}g/ml)$ of Lb. plantarum NO1 reduced $(40{\sim}60%)$ the urease activity of H. pylori KCCM 41756. Lb. plantarum NO1 inhibited the binding of H. pylori to human gastric cancer cell line, AGS cells, by more than 33%. Lb. plantarum NO1 exhibited high viability (maintained initial viable cell count of $10^9CFU/ml$) in 0.05 M sodium phosphate buffer (pH 3.0) for 2 h, in artificial gastricjuice for 2 h and in 0.3%, 0.5% oxgall for 24 h. Hemolysis phenomena did not observed when Lb. plantarum NO1 was incubated in the blood agar media. We concluded that Lb. plantarum NO1 can be a good candidate as a probiotic, harboring anti-H. pylori activity.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.3
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• pp.452-459
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• 2017

In recent years, to reduce self-weight of structural elements, the use of SCP (Steel Concrete Plate) instead of prestressed concrete is increasing. Because SCP has a complicated sectional shape and includes a large number of studs, the use of high-fluidity concrete is required. Therefore, in this study, to prevent the restrained shrinkage behavior by the stud, the effects of using an expansive agent (EA) and shrinkage reducing agent (SRA) were investigated, and the optimal mixture proportions to maximize the filling capacity were determined based on the fine aggregate ratio. The test results indicated that the combined use of EA and SRA exhibited the smallest shrinkage. The ratio of the crushed sand and washed sea sand was determined to be 5:5, and the proper fine aggregate ratio was found to be 55.6%, because when the ratio was too high, a decrease in strength and an increase in shrinkage strain were expected. The high-fluidity concrete effectively filled the large-sized SCP module.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.11
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• pp.604-609
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• 2016

This study evaluated the long-term performance of RSR (Reinforced Subgrade for Railways) technology which increases the railway line capacity without the need for additional land. Its characteristics include the use of a short reinforcement with rigid wall, which make it possible to apply it in confined spaces. The 7m high and 40m long testbed employed to evaluate the long-term performance was designed and constructed near Jupo station on the Chang-hang line. This line, located close to a local bus route, had collapsed at the subgrade following heavy rainfall. The performance of the new type of subgrade was verified with long term measurements over a 2 year period including the surface and ground settlement, horizontal displacement of the wall, tensile strain of the reinforcement, and settlement of the rail top on the side track. Based on the results of the measurements made until now, we concluded that it had sufficient safety and serviceability for use as a railway subgrade. It is expected that RSR technology could be frequently used at sites which lack the necessary construction materials for an embankment and are located close to functional railway lines and boundaries, in order to settle civil complaints.

• Journal of the Korean Society for Railway
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• v.10 no.6
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• pp.701-708
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• 2007

A tilting train, which was developed to run the curve section without reducing the speed and compromising the riding quality, can improve the speed so as to reduce the travel time, compared to the existing trains. Then the force generated by the train operation to the track is in proportion to train operation speed, which means the track shall bear the increased force as much as the increase in train operation speed. Particularly, wheel load and lateral wheel load generated by train operation and distributed to the rail tend to cause the track to suffer the strain and furthermore the severe disaster such as derailment. To deal with such problem and ensure the train will run safety and stably, the tolerance in wheel load change, lateral wheel load and derailment coefficient was determined for quantitative evaluation of the train operation stability. In this study, derailment coefficient of inner and outer rail at existing curve section of tilting train was determined to evaluate the curve radius, possibility of acceleration and the need of rail improvement, which was then compared with the existing traditional train and high speed train. Conducting the quantitative evaluation of dynamic wheel load and lateral wheel load of each train, which was based on field survey, derailment coefficient and static & dynamic wheel load change, which serve the evaluation criteria of train operation stability, were determined for comparison with the standards, thereby analyzing the stability of the tilting train.

• Journal of Veterinary Clinics
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• v.28 no.1
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• pp.101-107
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• 2011

This study aimed to determine dose-response (DR) curve of avian influenza (AI) virus to predict the probability of illness or adverse health effects that may result from exposure to a pathogenic microorganism in a quantitative microbial risk assessment. To determine the parametric DR relationship of several strains of AI virus, 7 feeding trial data sets challenging humans (5 sets) and chickens (2 sets) for strains of H3N2 (4 sets), H5N1 (2 sets) and H1N1 (1 set) from the published literatures. Except for one data set (study with intra-tracheal inoculation for data set no. 6), all were obtained from the studies with intranasal inoculation. The data were analyzed using three types of DR model as the basis of heterogeneity in infectivity of AI strains in humans and chickens: exponential, beta-binomial and beta-Poisson. We fitted to the data using maximum likelihood estimation to get the parameter estimates of each model. The alpha and beta values of the beta-Poisson DR model ranged 0.06-0.19 and 1.7-48.8, respectively for H3N2 strain. Corresponding values for H5N1 ranged 0.464-0.563 and 97.3-99.4, respectively. For H1N1 the parameter values were 0.103 and 12.7, respectively. Using the exponential model, r (infectivity parameter) ranged from $1.6{\times}10^{-8}$ to $1.2{\times}10^{-5}$ for H3N2 and from $7.5{\times}10^{-3}$ to $4.0{\times}10^{-2}$ for H5N1, while the value was $1.6{\times}10^{-8}$ for H1N1. The beta-Poisson DR model provided the best fit to five of 7 data sets tested, and the estimated parameter values in betabinomial model were very close to those of beta-Poisson. Our study indicated that beta-binomial or beta-Poisson model could be the choice for DR modeling of AI, even though DR relationship varied depending on the virus strains studied, as indicated in prior studies. Further DR modeling should be conducted to quantify the differences among AI virus strains.

• Journal of the Korean Wood Science and Technology
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• v.33 no.3 s.131
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• pp.72-78
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• 2005

The present study was conducted to evaluate the application of Chamaecyparis obtusa and to investigate potential utilization of essential oil from C. obtusa as plant-based medicine. The antifungal activity of essential oil from leaves and twigs of C. obtusa (Sieb. Et Zucc) was determined and the major components of active fractions against Microsporum canis (KCTC6591) and Trichophyton mentagrophytes (KCTC6077) were identified by GC/MS analysis. In treatment of essential oil from C. obtusa, the strain M. canis was more resistant than the other, T. mentagrophytes. In the agar diffusion assay, essential oil from C. obtusa inhibited hyphal growth of M. canis and T. mentagrophytes at the concentration of more than 5,000 ppm. The zones named B and C in the TLC assay of essential oil from C. obtusa showed antifungal activities. Among four sub-fractions of n-hexane extract from B and C zones, named as B-1, B-2, C-1 and C-2, the C-2 showed the highest antifungal activity. Instrumental GC/MS analysis for sub-fractions showed that a major component of C-1 was ${\alpha}$-terpineol as terpene alcohol, while C-2 contained sesquiterpenes such as elemol, cedrol and eudesmol.

• Journal of Sericultural and Entomological Science
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• v.51 no.2
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• pp.137-141
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• 2013

In this study, nucleotide sequence structures of intergenic transcribed spacer (ITS) 1, complete 5.8S ribosomal RNA gene and ITS 2 region were analyzed to identify three Peruvian entomopathogenic fungal isolates. The isolates had highly conserved sequence region in 5.8S rRNA gene and unique sequences in ITS 1 and 2 region among them. 5.8S rRNA gene regions were highly conserved and showed high homoloies among tested isolates. In contrast, ITS region showed species-specific sequence region, resulting in inter-genus differencies. Scanning electron microscopic images of these isolates supported the result of ITS-based identification. From these result, Peruvian entomopathogenic fungal isolate J270, J278, were identified as Beauveria bassiana and J271 was identified as Lecanicillium attenuatum.

• Korean Journal of Microbiology
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• v.41 no.2
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• pp.152-156
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• 2005

In the present study, lactic acid bacterium that has antibacterial activity against Helicobacter pylori was isolated from feces of newborn baby. The selection was based on the ability to inhibit the growth of H. pylori and to withstand harsh environmental conditions such as acidic pH and high bile concentration. By biochemical test and 16S rDNA sequencing, selected strain was turned out to be an Pediococcus acidilactici, therefore designated to P. acidilactici GMB7330. In order to investigate the inhibitory effects of P. acidilactici GMB7330 on the growth of H. pylori, we have tested in vitro studies such as cell viability and urease test. These results showed that antibacterial activity of P. acidilactici GMB7330 significantly decreased the viable cell count and urease activity of H. pylori. Antibacterial activity of P. acidilactici GMB7330 against H. pylori remained after pH adjustment to neutral, and the concentration of lactate produced from P. acidilactici GMB7330 was not enough to inhibit H. pylori. On the basis of the analysis by transmission electron microscope, it demonstrated that addition of P. acidilactici GMB7330 destroyed the cell structure of H. pylori. These results strongly suggested that P. acidilactici GMB7330 produce antibacterial substances to be able to inhibit the growth of H. pylori other than lactic acid.

• Journal of the Microelectronics and Packaging Society
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• v.21 no.4
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• pp.77-82
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• 2014

Stretchable electrodes are focused due to many demands for soft electronics. One of the candidates, carbon black composites have advantages of low cost, easy processing and decreasing resistivity in a certain range during stretching. However, the electrical conductivity of carbon black composites is not enough for electronic devices. Graphene is 2-dimensional nanostructured carbon based material which shows good electrical properties and flexibility. They may help to improve electrical conductivity of the carbon black composites. In this study, graphene was added to a carbon black electrode to enhance electrical properties and investigated. Electrical resistivity of graphene added carbon electrode decreased comparing with that of carbon black electrode because graphene bridged non-contacting carbon black aggregates to strengthen the conductive network. Also graphene reduced an increase in the resistance of the carbon black electrode applied to strain because they connected gap of separated carbon black aggregates and aligned along the stretching direction at the same time. In conclusion, an addition of graphene to carbon black gives two benefits on the electrical properties of carbon black composite as a stretchable electrode.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.16 no.3
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• pp.251-260
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• 2003

A three dimensional (3-D) method of analysis is presented for determining the free vibration frequencies and mode shapes of deep, tapered rods and beams with circular cross section. Unlike conventional rod and beam theories, which are mathematically one-dimensional (1-D), the present method is based upon the 3-D dynamic equations of elasticity. Displacement components u/sup r/, u/sub θ/ and u/sub z/, in the radial, circumferential, and axial directions, respectively, are taken to be sinusoidal in time, periodic in , and algebraic polynomials in the r and z directions. Potential (strain) and kinetic energies of the rods and beams are formulated, the Ritz method is used to solve the eigenvalue problem, thus yielding upper bound values of the frequencies by minimizing the frequencies. As the degree of the polynomials is increased, frequencies converge to the exact values. Convergence to four-digit exactitude is demonstrated for the first five frequencies of the rods and beams. Novel numerical results are tabulated for nine different tapered rods and beams with linear, quadratic, and cubic variations of radial thickness in the axial direction using the 3D theory. Comparisons are also made with results for linearly tapered beams from 1-D classical Euler-Bernoulli beam theory.