Journal of the Korean Wood Science and Technology

The Korean Society of Wood Science & Technology (한국목재공학회)
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Investigation of Active Antifungal Compounds of Essential Oil from Chamaecyparis obtusa Against Dermatophytes, Microsporum canis and Trichophyton Mentagrophytes

피부사상균 Microsporum canis 및 Trichophyton mentagrophytes에 대한 편백정유의 항진균활성물질 탐색

  • Park, Mi-Jin (Dept. of Forest Science, College of Agriculture & Life Science, Seoul national university) ;
  • Lee, Soo-Min (Dept. of Forest Science, College of Agriculture & Life Science, Seoul national university) ;
  • Gwak, Ki-Seob (Dept. of Forest Science, College of Agriculture & Life Science, Seoul national university) ;
  • Jeung, Eui-Bae (College of Veterinary Medicine, Chungbuk National University) ;
  • Chang, Je-Won (Enbita Co. LTD) ;
  • Choi, In-Gyu (Dept. of Forest Science, College of Agriculture & Life Science, Seoul national university)
  • 박미진 (서울대학교 농업생명과학대학 산림과학부) ;
  • 이수민 (서울대학교 농업생명과학대학 산림과학부) ;
  • 곽기섭 (서울대학교 농업생명과학대학 산림과학부) ;
  • 정의배 (충북대학교 수의과대학 수의학과) ;
  • 장제원 (엔바이타(주)) ;
  • 최인규 (서울대학교 농업생명과학대학 산림과학부)
  • Received : 2004.11.23
  • Accepted : 2005.01.17
  • Published : 2005.05.25
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Abstract

The present study was conducted to evaluate the application of Chamaecyparis obtusa and to investigate potential utilization of essential oil from C. obtusa as plant-based medicine. The antifungal activity of essential oil from leaves and twigs of C. obtusa (Sieb. Et Zucc) was determined and the major components of active fractions against Microsporum canis (KCTC6591) and Trichophyton mentagrophytes (KCTC6077) were identified by GC/MS analysis. In treatment of essential oil from C. obtusa, the strain M. canis was more resistant than the other, T. mentagrophytes. In the agar diffusion assay, essential oil from C. obtusa inhibited hyphal growth of M. canis and T. mentagrophytes at the concentration of more than 5,000 ppm. The zones named B and C in the TLC assay of essential oil from C. obtusa showed antifungal activities. Among four sub-fractions of n-hexane extract from B and C zones, named as B-1, B-2, C-1 and C-2, the C-2 showed the highest antifungal activity. Instrumental GC/MS analysis for sub-fractions showed that a major component of C-1 was ${\alpha}$-terpineol as terpene alcohol, while C-2 contained sesquiterpenes such as elemol, cedrol and eudesmol.

본 연구는 국내산 편백의 활용도를 높이고 편백정유의 식물성 의약품으로서 이용 가능성을 조사하고자 피부사상균인 Microsporum canis (KCTC6591), Trichophyton mentagrophytes (KCTC6077)에 대한 국내산 편백정유의 항진균활성을 평가하고, 활성분획의 주성분을 GC/MS로 확인하였다. 편백정유는 M. canis보다 T. mentagrophytes에 대한 항진균효과가 더 높게 나타났으며, 한천 희석법에서 5,000 ppm 이상의 농도로 처리시 M. canis와 T. mentagrophytes 모두 균사 생장을 못하였다. TLC를 이용한 편백정유 분획에서는 B와 C부분에서 활성이 나타났으며 B와 C의 하부분획에서는 C-2가 강한 항진균 활성을 보였다. 항진균활성을 보이는 C-1의 주성분은 ${\alpha}$-terpineol이고, C-2의 주성분은 elemol, cedrol, eudesmol임을 GC/MS 분석에 의해 확인하였다.

Keywords

References

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