• Journal of Mechanical Science and Technology
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• 제15권7호
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• pp.1002-1012
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• 2001

The work reported here is an extensive study of tendon response to multiple cyclic tests including 3% constant peak strain level test (A-type test), 3% constant peak strain level test with two rest periods (B-type test), and 3∼4% different peak strain level test (C-type test). A sufficient number of specimens were tested at each type of the test to statistically evaluate many changes in response during testing and differences in response between each type of the test. In cyclic tests, there were decreses (relaxations) in the peak stresses and hysteresis, increases in the slack strains, and during lower peak strain level (3%) cyclic block after higher peak strain level (4%) cyclic block in the C-type tests. Considering the results of this study and those of the other study of multiple cyclic tests with rest periods by Hubbard and Chun, 1985, recovery phenomena during the rest periods occurred predominantly at the beginning of the rest periods. Consistently in both studies, the effects of rest periods were small and transient compared to the effects of the cyclic extensions. The recovery with cycles at lower peak strain level (3%) after higher peak strain level (4%) in the C-type test has not been previously documented. This recovery seems to be a natural phenomena in tissue behavior so that collagenous structures recover during periods of decreased demand.

• 미생물학회지
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• 제28권1호
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• pp.83-89
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• 1990

토양으로부터 세포의 adcnosinedeaminase를 생산하는 두종의 방선균 J-845S주와 J-326TK 주름 분리하여 각각 Streptomycs sp. J-845S와 Nocardioides sp. J326TK로 동정하였다. Streptomyces sp. J-845S는 그람염색 양성, 비항산성균으로 기균사의 흰색계열의 간상형의 비운동성의 포자를 형성하였으며, 포자의 표면은 평할하였고 나선상의 포자연쇄를 형성하였다. 균사체의 분자는 양호하였다. 세포벽 구성성분을 분석한 결과 LL-diaminopimelic acid를 함유하는 cell wall type I 이었으며, phospholipid type II였다. Nocardioides sp. J-326TK는 그람염색반응 양성, 항산성염색반응 음성이었으며, 균사체는 불규칙한 간상 또는 구사으이 절편으로 분절되었다. 기균사의 분자는 그다지 인정되지 않았으며, 포자형태의 긴 elements의 표면은 평할하였다. 세포벽 구성성분을 검토한 결과 cell wall type I과 phospholipid type I으로나타났다.

• 한국기계가공학회지
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• 제13권4호
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• pp.75-82
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• 2014

A pin-type load-cell which uses shear-type strain gauges was developed to measure the tension of a wire in a winch. A finite element analysis was performed to determine the locations of the strain gauges. All of the shear-type strain gauges were attached onto parts that undergo regularly shear stress distributions. A Wheatstone bridge circuit was used to connect each of the gauges and to measure the strains. Linearity within the 5% error range was noted when testing the pin-type load-cell.

• Journal of Microbiology and Biotechnology
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• 제25권2호
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• pp.206-216
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• 2015

To clarify the role of surface protective antigen A (SpaA) in the pathogenesis of Erysipelothrix rhusiopathiae C43065 (serotype 2), the spaA deletion mutant of E. rhusiopathiae ${\Delta}spaA$ was constructed by homologous recombination. The virulence of the ${\Delta}spaA$ mutant decreased more than 76-fold compared with that of the wild-type strain C43065 in mice. The mutant strain was sensitive to the bactericidal action of swine serum, whereas the wild-type strain was resistant. The adhesion of wild-type strain to MEF cells was inhibited significantly by treatment with rabbit antiserum against recombinant SpaA (rSpaA) as compared with the treatment with normal rabbit serum, but the mutant strain was not affected. The mutant strain was readily taken up by mouse peritoneal macrophages in the normal rabbit serum, whereas the wild-type strain was resistant. Whereas the rabbit antiserum against rSpaA promoted the phagocytosis of wild-type strain by macrophages, the mutant strain was not affected. In addition, mice vaccinated with the formalin-killed mutant strain were provided 40% protection against challenge by the homologous virulent strain as compared with those with wild-type strain, NaOH-extracted antigen, or rSpaA, which provided more than 80% protection against the same infection. These suggested that SpaA has an important role in the pathogenesis of E. rhusiopathiae infection and could be a target for vaccination against swine erysipelas.

• 한국양식학회지
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• 제9권3호
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• pp.187-194
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• 1996

어류 종묘생산시 중요한 먹이생물로 사용되는 한국산 rotifer를 순수 분리하기 위하여 염전, 기수호, 하구 및 어류 양식장을 중심으로 채집하였다. 해수산 rotifer, B. plicatilis는 13 지역에서 L-type rotifer 2 strains과 S-type rotifer 14 strains을 순수 분리하였다. 담수산 rotifer, B. calyciflorus는 4곳의 양식장에서 4 strains을 순수 분리하였다. 순수 분리된 해수산 rotifer, B. plicatilis. L-type과 S-type rotifer strain의 피갑장과 내구란 장경의 분포는 각각 $244.3\~255.3\;{\mu}m,\;142.4\~145.5\;{\mu}m$와 $131.0\~165.8\;{\mu}m,\;93.7\~116.4\;{\mu}m$로 나타났다. 또 담수산 rotifer, B. calyciflorus strains의 피갑장과 내구란 장경의 분포는 각각 $211.8\~229.9\;{mu}m$와 $126.8\~140.2\;{\mu}m$로 나타났다. B. plicatilis는 성장과 내구란 생산이 매우 다양하게 나타났다. L-type과 S-type의 rotifer의 최고 밀도는 각각 O-L이 200개체/ml였고, H-S가 753.3개체/ml로 가장 높게 나타났다. 생산된 내구란은 S-type에서는 YY-S가 86.7개/ml로 가장 높았고, L-type에서는 O-L이 45.8개/ml로 높았다.

• 대한수의학회지
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• 제51권3호
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• pp.193-201
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• 2011

An attenuated vaccine strain AVR1/08 of Korean respiratory type of infectious bronchitis virus (IBV) was developed by 89th passages of IBV D85/06 strain in chicken eggs. The AVR1/08 strain had higher virus titer at least 20 times ($10^{1.3}$) than the parent virus D85/06 by egg inoculation method. The AVR1/08 strain had a single point mutation (S to Y) at position 56 of spike protein of IBV compared to parent virus IBV D85/06 strain. The mutation was observed consistently at viruses after 47th passage in chicken eggs. The AVR1/08 strain showed no virulence even after 6 passages in chickens and all chickens inoculated induced anti-IBV antibody 14 days after vaccination. The AVR1/08 strain had broad protective efficacy against QX type Korean nephropathogenic virus (Q43/06 strain), KM91 type Korean nephropathogenic virus (KM91 strain) and Korean respiratory virus (D85/06 strain). In contrast, Massachusetts (Mass) type attenuated vaccine strain H120 showed protection of 37.5 to 50% against these three viruses. Our results indicate that the AVR1/08 strain has potential as an attenuated vaccine effective in controlling IBVs circulating in Korea.

• Journal of Microbiology
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• 제44권1호
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• pp.113-120
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• 2006

Candida tropicalis was treated with ultraviolet (UV) rays, and the mutants obtained were screened for xylitol production. One of the mutants, the UV1 produced 0.81g of xylitol per gram of xylose. This was further mutated with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), and the mutants obtained were screened for xylitol production. One of the mutants (CT-OMV5) produced 0.85g/g of xylitol from xylose. Xylitol production improved to 0.87 g/g of xylose with this strain when the production medium was supplemented with urea. The CT-OMV5 mutant strain differs by 12 tests when compared to the wild-type Candida tropicalis strain. The XR activity was higher in mutant CT-OMV5. The distinct difference between the mutant and wild-type strain is the presence of numerous chlamvdospores in the mutant. In this investigation, we have demonstrated that mutagenesis was successful in generating a superior xylitol-producing strain, CT-OMV5, and uncovered distinctive biochemical and physiological characteristics of the wild-type and mutant strain, CT-OMV5.

• Journal of Plant Biology
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• 제1권1호
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• pp.1-6
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• 1958

1. Study was made to investigate effects of desoxyribonucleates on copper-resistance in yeast. 2. It is found that the resistant strain there exists the phenomic lag. 3. In the occurrence of the resistant strain there exists the phenomic lag. 4. Desoxyribonucleate isolated from copper resistant culture is capable of inducing the resistant strain, which is the same type as donor of the resistant type. It accelerated the rate of variation to the resistant, but it is of no effect on the resistant strain. 5. Desoxyribonucleate derived from non-resistant type inhibits growth of the resistant strain and delays the initial phase of growth. However, it is of no effect on the sensitive strain. It is concluded that desoxyribonucleate derived from resistant culture is capable of inducing the resistance, however, nonresistant type desoxyribonucleate is of no effect on inducing the resistance.

• 한국균학회지
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• 제34권2호
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• pp.88-91
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• 2006

가평에서 낮은 생산성을 보이는 저온성 품종과 고온성 품종의 균사상태 및 골목 표면에 형성된 해균에 대한 조사를 시도했다. 순수배지에서의 균사생장력을 조사한 결과, 저온성 균주는 분리균에 비해 보존균의 생장력이 1.1% 정도 떨어지는 것으로 나타났으며, 고온성 균주는 분리균의 생장력이 보존균의 생장력에 비해 8.0% 정도 떨어지는 것으로 나타났다. 톱밥배지에서의 균사생장력을 조사한 결과, 고온성 분리균은 보존균에 비해 10.8%정도 생장력이 떨어졌으며, 저온성 분리균은 보존균에 비해 25.1% 떨어지는 것으로 나타났다. 중량감소율 조사결과, 고온성 분리균은 보존균에 비해 20.1% 정도 중량감소율이 낮았으며, 저온성 분리균도 보존균에 비해 19.0%정도 낮은 것으로 나타났다. 무처리에 비해서는 고온성 보존균이 107.0%, 고온성 분리균이 49.5%, 저온성 보존균이 85.4%,저온성 분리균이 50.0% 더 감소된 것으로 나타났다. 대치배양한 결과, 고온성 분리균과 저온성 분리균 모두 각각의 해당 보존균들과 동일한 균주임을 확인할 수 있었다. 또한 골목에서 검은혹버섯(Hypoxylon truncatum), 구름버섯(Coriolus versicolor), 기와층버섯(Inonotus xeranticus), 삼색도장버섯(Daedaleopsis tricolor), 이중껍질버섯(Graphostroma platystoma), 점균류(Myxomycetes) 2종, 푸른 곰팡이(Trichoderma sp.), Hypoxylon fragiforme, Hypoxylon howeianum, Nitschkia confertula 등 총 11종의 해균이 발견되었으며, 골목들의 상태는 좋지 않았다.

• 대한조선학회지
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• 제7권2호
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• pp.27-40
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• 1970

The distribution of stress and strain in elastic stages is investigated by the experiments of two dimensional photoelastic coating and Moire fringe method. Center block type and cover plate type of fillet welds are used as specimens in the test. The results are as follows. 1) Center block type gets less uniform stress distribution than cover plate type. And its stress concentration factor, especially at root, is larger than that at toe. 2) When main plate and cover plate closely contact and it cause friction, stress concentration decreases more than that in case of slit. That is because stress can be transmitted on the contact surface. 3) When slit is made, the outside of fillet gets more stress than the inside of it. 4) While the plastic strain distribution of center block type reaches the maximum at root and differs very slightly from that under lower loading, the plastic strain distribution of cover plate type is inclined to get the maximum at the outside of fillet rather than at root. 5) When the plastic strain value of cover plate type is compared with that of center block type at toe and root, the relations between the former and the latter shows root<toe and root>toe. 6) Because stress distribution becomes changed according to loading, fracture angle cannot be estimated by the peaks of elastic stress distribution. 7) The strain distribution just before fracture can be found by Moire fringe method.