• BMB Reports
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• v.33 no.4
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• pp.353-357
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• 2000

A new assay method of ${\alpha}-Oxidase$ (fatty acid : oxygen dioxygenase, 1-decarboxylating) was developed using a bioluminescence reaction system of marine luminous bacterium, Photobacterium phosphoreum. ${\alpha}$-Oxidase was isolated from a cucumber (Cucumis sativus). Pentadecanoic acid was used as a substrate, and the product, tetradecanal, was analyzed with a bacterial luciferase-coupled reaction. Initial light intensity was directly related to the concentration of tetradecanal in the range of 1 nM to 10 ${\mu}M$. Optimal pH and temperature were 7.5 and $25^{\circ}C$, respectively. Optimal pentadecanoic acid concentration in a standard assay of ${\alpha}$-oxidase was 0.1 mM. The Km value of pentedecanoic acid was $85{\mu}M$. This method is straightforward, rapid, convenient, and easy. Its needs no treatment or extraction of reaction mixture.

• Journal of computational fluids engineering
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• v.9 no.3
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• pp.73-80
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• 2004

A LS(Level Set) formulation is developed for computing two-phase flows on non- orthogonal meshes. Compared with the VOF(Volume-of-Fluid) method based on a non-smooth volume-fraction function, the LS method can calculate an interfacial curvature more accurately by using a smooth distance function. Also, it is quite straightforward to implement for 3-D irregular meshes compared with the VOF method requiring much more complicated geometric calculations. The LS formulation is implemented into a general purpose program for 3-D flows and verified through several test problems.

• Korean Institute of Interior Design Journal
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• v.13 no.4
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• pp.127-137
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• 2004

Since 20th century, the exhibition has expanded to more diverse fields and recognized as the medium which can build network among the Art, Human and society. Space design, although developed within mutual interaction and consideration of environment factors, has been treated without those background. Therefore it is straightforward to examine the exhibition space design synthetically not limit it by the analytical elements. Assuming three main mutual interactions, object human, and environment, we have attempt the typological analysis to the museum exhibition space by studying characteristics. While the exhibition space design has been analyzed through two dimensional interpretation, on this study, we structuralize diverse discussion of the exhibition space design by relation-centered and relative analysis. Therefor we examine the characteristic of design expression through typology of both physical and behavioral feature. In the conclusion, the outcome provides insights into the relationships among object, human and environment and useful measurements to designer who outline exhibition space design.

• IE interfaces
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• v.17 no.4
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• pp.407-413
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• 2004

Recently, many enterprises are adopting EAI (Enterprise Application Integration) technologies for integrating heterogeneous enterprise information systems. Among EAI levels, data-level integration is relatively straightforward and most popular. However, most commercial solutions provide complex functionalities and are expensive for implementing the data integration tasks at the small & medium enterprises. Also, they have their own proprietary architectures and have a restricted interoperability. Proposed in this paper is the development of a data integration middleware for facilitating data exchanges between the heterogeneous information systems. The main feature of this middleware is a explicit mapping of meta data about the relationships between source and target data. Based on this explicit mapping, users who do not have expertise in information technology at the small & medium enterprises can easily execute data exchange tasks among various information systems.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.9 no.6
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• pp.519-525
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• 2016

This paper addresses the framework of object-oriented image coding, describing a new algorithm, based on monodimensional Legendre polynomials, for texture approximation. Through the use of 1D orthogonal basis functions, the computational complexity which usually makes prohibitive most of 2D region-oriented approaches is significantly reduced, while only a slight increment of distortion is introduced. In the aim of preserving the bidimensional intersample correlation of the texture information as much as possible, suitable pseudo-bidimensional basis functions have been used, yielding significant improvements with respect to the straightforward 1D approach. The algorithm has been experimented for coding still images as well as motion compensated sequences, showing interesting possibilities of application for very low bitrate video coding.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 1998.04a
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• pp.407-412
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• 1998

In this work, a new time integration method is proposed using the generalized derivative concept to simulate the dynamic phenomena having sudden constraint occurring in dynamic contact/impact problems. By the adoption of the generalized derivative concept and jump assumption, discontinuity can be incorporated in time integration and as a result, the algorithm does not need any other special consideration of jumps in dynamic field variables due to sudden constraint like dynamic contact-release conditions. To observe the characteristics of the proposed time integration method, the stability and convergence analyses are carried out. In numerical tests, several dynamic contact/impact problems are analyzed by straightforward application of the proposed time integration method with the exterior penalty method.

• Journal of Mechanical Science and Technology
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• v.19 no.3
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• pp.811-819
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• 2005

In this study, we present a new efficient hybrid-mixed composite laminated curved beam element. The present element, which is based on the Hellinger-Reissner variational principle and the first-order shear deformation lamination theory, employs consistent stress parameters corresponding to cubic displacement polynomials with additional nodeless degrees in order to resolve the numerical difficulties due to the spurious constraints. The stress parameters are eliminated and the nodeless degrees are condensed out to obtain the ($6{\times}6$) element stiffness matrix. The present study also incorporates the straightforward prediction of interlaminar stresses from equilibrium equations. Several numerical examples confirm the superior behavior of the present composite laminated curved beam element.

• Proceedings of the Korean Operations and Management Science Society Conference
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• 2000.04a
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• pp.385-388
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• 2000

The computation of a terminal-pair reliability is started with a Boolean sum of products expression corresponding to simple paths of the pair of nodes. This expression is then transformed into another equivalent expression to be a Sum of Disjoint Products form. The terminal reliability can then be obtained in a straightforward manner from the Sum of Disjoint Products form. In this paper, we proposed an efficient method to obtain reliability expressions for calculating multiple K terminal-pairs reliability between terminal-pairs of nodes of computers of complex networks. Actual programming results show that the new method is superior with regard to computational efficiency, especially for computing the reliability of multiple terminals.

• Structural Engineering and Mechanics
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• v.36 no.2
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• pp.225-241
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• 2010

In this study, a hybrid search algorithm combining genetic programming with orthogonal least squares (GP/OLS) is utilized to generate prediction models for compressive strength of high performance concrete (HPC) mixes. The GP/OLS models are developed based on a comprehensive database containing 1133 experimental test results obtained from previously published papers. A multiple least squares regression (LSR) analysis is performed to benchmark the GP/OLS models. A subsequent parametric study is carried out to verify the validity of the models. The results indicate that the proposed models are effectively capable of evaluating the compressive strength of HPC mixes. The derived formulas are very simple, straightforward and provide an analysis tool accessible to practicing engineers.

• Structural Engineering and Mechanics
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• v.27 no.5
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• pp.555-574
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• 2007

Static response of an elastic beam on a two-parameter tensionless foundation is investigated by assuming that the beam is symmetrically subjected to a uniformly distributed load and concentrated edge loads. Governing equations of the problem are obtained and solved by pointing out that a concentrated edge foundation reaction in addition to a continuous foundation reaction along the beam axis in the case of complete contact and a discontinuity in the foundation reactions in the case of partial contact come into being as a direct result of the two-parameter foundation model. The numerical solution of the complete contact problem is straightforward. However, it is shown that the problem displays a highly non-linear character when the beam lifts off from the foundation. Numerical treatment of the governing equations is accomplished by adopting an iterative process to establish the contact length. Results are presented in figures to demonstrate the linear and non-linear behavior of the beam-foundation system for various values of the parameters of the problem comparatively.