• 한국발생생물학회지:발생과생식
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• 제14권4호
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• pp.261-268
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• 2010

As in the O. mykiss electrophoretic profiles of RNA, the signals of each RNA sample from 9 individual tissues such as liver, muscle, brain, heart, pituitary gland, kidney, intestine, spleen and gill similar to positive control were obtained. The tissue distributions of the complimentary DNA (cDNA) of O. mykiss four genes were analyzed using quantitative real-time PCR with primer sets for tissue expression analysis. In this rainbow trout species, author obtained bands of various sizes, ranged from 700 bp to 1,400 bp. A dissociation curve was made at the end of each run to make sure that there was no non-specific amplification. Supplementarily, the Ct of each DNA was compared. The Ct values of vinculin with rainbow trout tissues were determined in a manner similar to those for agouti-related protein (AgRP) and melanocortin receptors (MC4R I and MC4R II). Further, obtained Cts for standard curve of each DNA were affected by specific product (vinculin, AgRP and MC4R II genes). After several experiments with four individual genes of rainbow trout, author estimated a variation ratio of the mean Ct value of the DNA extracted using the comparative CTt method was 37.27, and the standard deviation was 5.33. The correlation coefficient between the Ct values and the concentration of cDNA was -0.98, -0.99, -0.91 and -0.86, respectively (vinculin, AgRP, MC4R I and MC4R II genes). Since this correlation showed high linearity, the straight line obtained was used as a standard for the O. mykiss tissues reared in aquarium. A PCR efficiency of 100% is ideally achieved when the slopes are close to the theoretical value of -3.31. According to quantification method, the results of quantification are strongly affected by the DNA fragmentation. The size of most DNA fragments obtained from various tissues of rainbow trout used in the experiment was approximately 100 bp. According to the four slopes, an efficiency of nearly 100% was estimated for four genes detection methods. Additionally, further analysis with more individuals and primers will be required to fully establish optimization in rainbow trout.

• 대한전자공학회논문지SP
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• 제45권2호
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• pp.29-36
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• 2008

본 논문에서는 취득된 영상으로부터 실세계 지시 영역을 추정하는 방법을 제안한다. 일반적으로 사람은 무엇인가를 지시할 경우, 얼굴 방향을 목표물에 두게 된다. 따라서 얼굴영역과 손끝 영역을 연결한 직선을 지시방향이라 간주하였다. 먼저, 제안된 알고리듬은 2대의 카메라로부터 실시간으로 얻어진 영상에서 피부색 검출을 통해 사용자의 얼굴 및 손끝영역에서 2점을 추출하고, 이를 실세계 3차원 기하학 정보를 이용하여 지시방향 및 지시영역을 추정한다. 또한 제안된 알고리듬을 이용한 정확한 지시영역 추출을 기반으로 두 대의 카메라와 한 대의 빔 프로젝터로 구성된 '양방향 영화정보 안내 시스템(ICIGS: Interactive Cinema Information Guiding System)'을 구성하여 제안된 알고리듬의 실용화 가능성을 확인하였다.

• 상하수도학회지
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• 제31권5호
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• pp.459-469
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• 2017

A real scale leakage test facility was developed to study the leak signal characteristics of water supply pipelines, and then leak tests were carried out. The facility was designed to overcome the limited experimental circumstances of domestic water supply pipeline experimental facilities. The length of the pipeline, which was installed as a straight line, is 280m. Six pipes were installed on a 70m interval with different pipe material and diameters that are DCIP(D200, D150, D100, D80), PE(D75) and PVC(D75).The intensity of the leakage is adjusted by changing the size of the leak hole and the opening rate of ball valve. Various pressure conditions were simulated using a pressure reducing valve.To minimize external noise sources which, deteriorate the quality of measured leak signal, the facility was built at a quiet area, where traffic and water consumption by customers is relatively rare. In addition, the usage of electric equipment was minimized to block out noise and the facility was operated using manual mode. From the experimental results of measured leakage signal at the facility, it was found that the signal intensity weakened and the signal of high frequency band attenuated as the distance from the water leakage point increased.

• 한국통신학회논문지
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• 제41권10호
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• pp.1247-1255
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• 2016

소형드론의 상용화를 위해서는 안전성과 자율운행기능의 확보가 필수적이다. 최근 드론제작이 상당히 용이해졌으나, 여전히 안정적인 드론의 제작은 쉽지 않다. 따라서 자체드론제작 필요성은 영상이나 자율이동 등 상위 알고리즘의 연구에 큰 장애요소로 존재한다. 본 연구에서는 상용드론과 Raspberry PI, 및 오픈소스를 활용하여, 쿼드로터 드론의 자율운행기술 개발 중 영상기반 자율운행을 설계해볼 수 있는 지상원격제어시스템(GCS)을 설계하고 구현하였다. 설계한 시스템은 모듈화된 구성으로 통신, UI 및 영상처리 모듈로 구성하였고, 특히 주행선유지 알고리즘을 구현하여 기능 및 성능 실험을 하였다. 설계한 주행선유지 알고리즘은 Hough 변환에 의하여 검출된 차선을 소실점 검출과 자제적인 라인트래킹 알고리즘을 개발하여 사용하여 인식오류를 줄였으며, 주행선과 드론의 진행방향을 계산하고 방향 (전진, 정지, 좌우회전)제어하였다. 구현된 시스템은 현재 100m육상트랙의 직선과 완만한 곡선을 2-3 m/s로 주행할 수 있다.

• 대한핵의학회지
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• 제21권2호
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• pp.143-150
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• 1987

To assess the analytic performance of immunoradiometric TSH assay (IRMA TSH), assay precision determined by intra and interassay variance, assay accuracy determined by dilution and recovery study, were evaluated by using two commercial kit $(Abott^{(R)}\;and\;Daichi^{(R)})$. Normal range of basal serum TSH and TRH stimulated TSH increment were also determined in 234 healthy subjects (male 110, female 124; age 20-70) and 30 voluteers (male 10, female 20; age 21-26). In addition, basal TSH levels of 70 patients with untreated hyperthyroidism, 50 untreated hypothyroidism, and 60 euthyroidism were measured to assess the clinical utility of IRMA TSH. The detection limit of IRMA TSH was 0.04 mU/l and 0.08 mU/l by Abott Kit and Daichi kit respectively. Using Abott kit, intra assay variance were 2.0, 3.1 and 1.4% in mean TSH concentration 2.4, 31.6 and 98.2 mU/l repectively and interassay variance were 2.0 and 3.2% in mean TSH concentration 2.3 and 31.3 mU/l. Mean recovery rate was 92.5% and dilution study showed nearly straight line. When Daichi kit was used, intrasssay variance were 5.6, 5.2 and 6.2% in mean TSH concentration of 2.4, 31.6 and 98.2 mU/l respectively and interassay variance were 7.1 and 7.4% in mean TSH of 2.3 and 31.3 mU/l. Mean recoveray rate was 89.9%. Normal range of basal TSH and TRH stimulated peak TSH were 0.38-4.02 mU/l and 2.85-30.8 mU/l repectively (95% confidence interval, Abott kit used). Sensitivity and specificity of basal TSH levels for diagnosing hypothyroidism as well as specificity for diagnosing hyperthyroidism were 100% by using both kit. Sensitivity of basal TSH level for diagnosing hyperthyroidism was 100% when TSH levels were measured by Abott kit while that was 80.9% when measured by Daichi kit. These results suggest that IRMA TSH was very precise and accurate method and might be used as a first line test in the evaluation of thyroid function.

• 한국지능시스템학회논문지
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• 제18권5호
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• pp.624-631
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• 2008

본 논문은 이동 로봇이 움직이는 목표물을 실시간으로 따라가게 하는 방법을 제안한다. 로봇은 이동하는 목표물을 일정한 방향과 거리를 유지하면서 따라간다. 이 방법은 다음의 두 단계로 이루어진다. 첫 번째 단계에서는 목표물의 위치를 로봇 좌표계 상에서 구해낸다. 두 번째 단계에서는 목표물을 따라가기 위한 로봇의 직진 속도와 회전 속도를 구해낸다. 목표물의 위치를 구하기 위해 영역 센서 데이터를 히스토그램으로 나타낸다. 실시간으로 계산된 로봇 좌표계에서의 목표물의 위치정보를 사용하여 목표물을 따라가게 하는 로봇의 직진 속도와 회전 속도를 구한다. 로봇의 직진 속도와 회전 속도는 로봇의 목표물로의 방향과 목표물까지의 거리를 원하는 값으로 수렴할 수 있게 한다. 제안된 방법의 성능을 시뮬레이션을 통하여 검증하였다. 시뮬레이션에서 목표물은 직선 궤적, 직사각형 궤적, 그리고 원 궤적에 의해 움직이게 하였다. 시뮬레이션결과 목표물이 급격히 방향을 바꾸는 경우에는 순간적으로 목표물 추적이 불가능함을 알 수 있는데, 이것은 실시간 추적에서는 피할 수 없는 문제이다. 그렇지만, 이 경우에도 로봇이 빠른 속도로 목표물을 추적하여 다시 따라잡게 된다. 제안된 방법은 로봇이 목표물을 따라가도록 하는 경우에는 물론 여러 대 로봇이 대형을 갖추어 이동하게 하는 경우에도 적용도 가능하다.