• 한국균학회지
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• 제27권5호
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• pp.349-353
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• 1999

현재까지 국내에 보고된 꽃구름버섯은 도합 5종으로서 그중 4종은 흰테꽃구름버섯(Stereum gausapatum), 꽃구름버섯(S. hirsutum), 갈색꽃구름버섯(S. ostrea), 및 배착꽃구름버섯(S. ochraceo-flavum)이며, 나머지 1종은 거북꽃구름 버섯속에 속하는 너털거북꽃구름버섯(Xylobolus spectabile)으로서 낭상체의 형태적 특징에 의하여 거북꽃구름버섯속(Xylobolus)에 속하여 왔으나, Boidin과 Chamuris는 배양학적 특징에 의하여 후자를 꽃구름버섯속(Stereum)으로 분류하고 있다. 본 연구에서는 "너털거북꽃구름버섯"(X. spectabile)을 꽃구름버섯속에 재분류하여 분류체계를 개선하고 우리말 이름을 "너털꽃구름버섯"(S. spectabile)으로 개칭하는 바이다. 또한 서울대학교 표본실(Seoul National University Fungal Collection, SFC)에 보관되어 있는 꽃구름버섯을 대상으로 조사한 결과 5종의 꽃구름버섯종들이 미기록종으로 동정되었으며, 이들 미기록종은 Stereum subtomentosum(갈색털꽃구름버섯, 신칭), S. peculiare(껍질 꽃구름버섯, 신칭), S. sanguinolentum(유혈꽃구름버섯, 신칭), S. striatum(줄무늬꽃구름버섯, 신칭), 및 S. complicatum(복합꽃구름버섯, 신칭)이었다. 이로서 한국산 꽃구름버섯 종들은 도합 10종에 이르며 본 연구를 통하여 국내 꽃구름 버섯의 동정을 위하여 새로운 검색표를 제시하였다.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1998년도 Proceedings of UNESCO-internetwork Cooperative Regional Seminar and Workshop on Bioassay Guided Isolation of Bioactive Substances from Natural Products and Microbial Products
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• pp.131-131
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• 1998

In the course of screening for new natural compounds having antioxidant activity from microbials, mushrooms as well as plants. We have isolated novel sesquiterpenoid and officinalic acid from methanolic extracts of Stereum ostrea. The dried fruit body( 450 g) of Stereum ostrea was extracted with methanol, then methanolic extract was partitioned between water and hexane, chloroform, and ethyl acetate, subsequently. The chloroform extract was subjected on silica gel, Sephadex LH-20 and RP-18 column chromatography, successively. And two compounds were isolated and identified as new triterpenoids and officinalic acid using $^1$H - NMR, $\^$13/C - NMR as well as Mass spectra.

• Journal of Applied Biological Chemistry
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• 제48권1호
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• pp.38-41
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• 2005

Structurally new antioxidative metabolite was isolated from mycelial culture of mushroom Stereum hirsutum. Culture broth was subjected to Diaion HP-20 column chromatography, and 70% aqueous MeOH eluent was extracted with EtOAc. EtOAc extract was purified through silica gel and Sephadex LH-20 column chromatographies, and reversed phase $C_{18}$ HPLC. Compound was revealed to be new dihydroxylated derivative of sterin B with molecular formula of $C_{12}H_{16}O_5$(MW 240) by MS and various NMR spectral data analyses, and designated as sterin C. Sterin C showed superoxide radical-scavenging activity with $EC_{50}$ value of 0.31 mM.

• Mycobiology
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• 제43권3호
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• pp.297-302
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• 2015

Two white rot fungi, Ceriporia sp. ZLY-2010 (CER) and Stereum hirsutum (STH) were used as biocatalysts for the biotransformation of (-)-${\alpha}$-pinene. After 96 hr, CER converted the bicyclic monoterpene hydrocarbon (-)-${\alpha}$-pinene into ${\alpha}$-terpineol (yield, 0.05 g/L), a monocyclic monoterpene alcohol, in addition to, other minor products. Using STH, verbenone was identified as the major biotransformed product, and minor products were myrtenol, camphor, and isopinocarveol. We did not observe any inhibitory effects of substrate or transformed products on mycelial growth of the fungi. The activities of fungal manganese-dependent peroxidase and laccase were monitored for 15 days to determine the enzymatic pathways related to the biotransformation of (-)-${\alpha}$-pinene. We concluded that a complex of enzymes, including intra- and extracellular enzymes, were involved in terpenoid biotransformation by white rot fungi.

• 한국응용약물학회:학술대회논문집
• /
• 한국응용약물학회 1998년도 Proceedings of UNESCO-internetwork Cooperative Regional Seminar and Workshop on Bioassay Guided Isolation of Bioactive Substances from Natural Products and Microbial Products
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• pp.130-130
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• 1998

Two novel compounds SSC1 and SSC2 were isolated from culture broth produced from the strain of Stereum hirsutum by using of YM broth. They were isolated through HP-20 column chromatography, silica gel column chromatography and preparative HPLC, successively. The molecular formulas of SSC1 and SSC2. were determined as C$\sub$15/H$\sub$22/O$_3$ by high resolution EI -mass. The chemical structures of SSC1 and SSC2 were determined as sesquiterpenoid compounds by spectroscopic analysis of UV, IR, $^1$H NMR, $\^$13/C NMR, DEPT, HMQC and HMBC spectrum.

• 한국균학회지
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• 제5권2호
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• pp.17-20
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• 1977

1974년(年)부터 1976년(年)까지 소백산(小白山), 안동(安東), 의성(義城)(고운사(孤雲寺))에서 채집(採集)한 고등균류(高等菌類)를 동정(同定)한 결과(結果), 한국미기록종(韓國未基錄種)은 다음과 같다. Lypophyllum fallax (Peck.) $K{\ddot{u}}hn$, et Romagn. 황토색만가닥버섯, Armillariella tabescens (Scop. ex Fr.) Sing. 미류나무버섯, Crepidotus subphaerosporus (Lange) $K{\ddot{u}}hn$. et Romagn. 주걱귀버섯, Crepidotus radians (Desm.) Fr. 노랑먹물버섯, Stereum hiugense Imaz. 털깍지버벗, Hygrophorus suzukaensis Hongo 주홍갓버섯, Xylaria carpophila(Pers.) Fr. 젓가락버섯.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1999년도 한국생물과학협회 학술발표대회
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• pp.60.2-60
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• 1999

No Abstract, See Full Text

• Journal of Microbiology and Biotechnology
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• 제22권11호
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• pp.1540-1548
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• 2012

Manganese peroxidase (MnP) was isolated from the culture filtrate of the wood log mushroom Stereum ostrea (S. ostrea), grown on Koroljova medium, and then purified by ammonium sulfate [70% (w/v)] fractionation, DEAE-cellulose anion exchange chromatography, and Sephadex G-100 column chromatography, with an attainment of 88.6-fold purification and the recovery of 22.8% of initial activity. According to SDS-PAGE the molecular mass of the MnP was 40 kDa. The optimal pH and temperature were found to be 4.5 and $35^{\circ}C$, respectively. The enzyme was stable even after exposure to a pH range of 4.5 to 6.0, and at temperatures of up to $35^{\circ}C$ at a pH of 4.5 for 1h. The $K_m$ and $V_{max}$ values for the substrate phenol red were found to be $8{\mu}m$ and 111.14 U/mg of protein, respectively. The MnP also oxidized other substrates such as guaiacol, DMP, and veratryl alcohol. Sodium azide, EDTA, SDS, $Cu^{2+}$, and $Fe^{2+}$, at 1-5 mM, strongly inhibited enzyme activity, whereas $Ca^{2+}$ and $Zn^{2+}$ increased enzyme activity. The participation of the purified enzyme in the decolorization of dyes suggests that S. ostrea manganese peroxidase could be effectively employed in textile industries.

• 임산에너지
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• 제20권1호
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• pp.6-11
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• 2001

한지의 원료가 되는 닥섬유의 펄프화를 위해 인피부에 목질 분해균을 처리하여 인피섬유의 단섬유화를 위한 미생물의 분해특성과 처리시간별 인피섬유의 분리에 대하여 검토하였다. 판막버섯이나 구름버섯으로 20일간 처리시 중량감소율이 약 50%에 이르러 오히려 이용 가능한 섬유소의 분해도 함께 초래하는 것으로 추정할 수 있다. 그러나 꽃구름버섯이나 벽돌빛뿌리 버섯은 인피섬유의 제일 외피에 존재하는 흑청색 부분을 잘 본해시키고 섬유소 손상을 적게하는 특성을 가진 것으로 나타났다. 꽃구름버섯은 처리 10일째가지 78.9%의 셀룰로오스 함량을 유지하고, 리그닌은 7.2%를 나타내어 제일 양호한 분해 양상을 보였다. 현미경적 관찰의 결과에서는 느타리버섯의 경우 처리 30일부터 인피부의 섬유가 분리되어 단섬유화 되며, 50일간 장기간 처리하여도 섬유표면의 손상은 발견되지 않았다.

• Mycobiology
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• 제35권4호
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• pp.210-214
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• 2007

Antibacterial and antifungal activities of liquid culture filtrate, water and ethanol extract (solid culture) of Stereum ostrea were evaluated against 5 bacteria and 3 plant pathogenic fungi. To determine the minimal inhibitory concentration (MIC), we studied $5{\sim}300\;mg/ml$ concentrations against bacteria and fungi separately. The MIC was 10 mg/ml for Bacillus subtilis and 40 mg/ml for Colletotrichum gloeosporioides and Colletotrichum miyabeanus. Liquid culture filtrate was more effective against Gram positive than Gram negative bacteria, and Staphylococcus aureus was the most inhibited (20.3 mm) bacterium. Water and ethanol extracts were effective against both Gram positive and Gram negative bacteria, and water extract was better than ethanol extract. In water and ethanol extract, inhibition zones were 23.6 and 21.0 mm (S. aureus) and 26.3 and 22.3 mm (Pseudomonas aeruginosa), respectively. For plant pathogenic fungi, the highest and lowest percent inhibition of mycelial growth (PIMG) was found 82.8 and 14.4 against C. miyabeanus and Botrytis cinerea in liquid culture filtrate, respectively. In water extract, the PIMG was found to be the highest 85.2 and lowest 41.7 for C. miyabeanus and C. gloeosporioides, respectively. The inhibitory effect of ethanol extract was better against C. miyabeanus than C. gloeosporioides and B. cinerea. Among 3 samples, water extract was the best against tested pathogenic fungi. This study offers that the extracts isolated from S. ostrea contain potential compounds which inhibit the growth of both bacteria and fungi.