Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Sterin C, a New Antioxidant from the Mycelial Culture of the Mushroom Stereum hirsutum

  • Yoo, Nam-Hee (Korea Research Institute of Bioscience and Biotechnology) ;
  • Yoo, Ick-Dong (Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Jin-Woo (Korea Research Institute of Bioscience and Biotechnology) ;
  • Yun, Bong-Sik (Korea Research Institute of Bioscience and Biotechnology) ;
  • Ryoo, In-Ja (Korea Research Institute of Bioscience and Biotechnology) ;
  • Yoon, Eui-Soo (Department of Biology, Kongju National University) ;
  • Chinh, Nguyen Thi (Department of Microbiology, Hanoi University of Science) ;
  • Kim, Jong-Pyung (Korea Research Institute of Bioscience and Biotechnology)
  • Published : 2005.03.31
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Abstract

Structurally new antioxidative metabolite was isolated from mycelial culture of mushroom Stereum hirsutum. Culture broth was subjected to Diaion HP-20 column chromatography, and 70% aqueous MeOH eluent was extracted with EtOAc. EtOAc extract was purified through silica gel and Sephadex LH-20 column chromatographies, and reversed phase $C_{18}$ HPLC. Compound was revealed to be new dihydroxylated derivative of sterin B with molecular formula of $C_{12}H_{16}O_5$(MW 240) by MS and various NMR spectral data analyses, and designated as sterin C. Sterin C showed superoxide radical-scavenging activity with $EC_{50}$ value of 0.31 mM.

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