• The Korean Journal of Mycology
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• v.27 no.5 s.92
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• pp.349-353
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• 1999

The genus Stereum is consisted of species having smooth, binucleate amyloid spores, pseudocystidia and dimitic basidiocarps without clamps. There are five recorded species of Stereum in Korea. Through the specimen examination of Seoul National University Fungal Collection, five more species of Stereum, S. subtomentosum, S. peculiare, S. sanguinolentum, S. striatum and S. complicatum, were confirmed as unrecorded species to Korea. They are registered here with Korean names as well as English descriptions and a key to Korean Stereum species is attached together.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1998.11a
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• pp.131-131
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• 1998

In the course of screening for new natural compounds having antioxidant activity from microbials, mushrooms as well as plants. We have isolated novel sesquiterpenoid and officinalic acid from methanolic extracts of Stereum ostrea. The dried fruit body( 450 g) of Stereum ostrea was extracted with methanol, then methanolic extract was partitioned between water and hexane, chloroform, and ethyl acetate, subsequently. The chloroform extract was subjected on silica gel, Sephadex LH-20 and RP-18 column chromatography, successively. And two compounds were isolated and identified as new triterpenoids and officinalic acid using $^1$H - NMR, $\^$13/C - NMR as well as Mass spectra.

• Journal of Applied Biological Chemistry
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• v.48 no.1
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• pp.38-41
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• 2005

Structurally new antioxidative metabolite was isolated from mycelial culture of mushroom Stereum hirsutum. Culture broth was subjected to Diaion HP-20 column chromatography, and 70% aqueous MeOH eluent was extracted with EtOAc. EtOAc extract was purified through silica gel and Sephadex LH-20 column chromatographies, and reversed phase $C_{18}$ HPLC. Compound was revealed to be new dihydroxylated derivative of sterin B with molecular formula of $C_{12}H_{16}O_5$(MW 240) by MS and various NMR spectral data analyses, and designated as sterin C. Sterin C showed superoxide radical-scavenging activity with $EC_{50}$ value of 0.31 mM.

• Mycobiology
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• v.43 no.3
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• pp.297-302
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• 2015

Two white rot fungi, Ceriporia sp. ZLY-2010 (CER) and Stereum hirsutum (STH) were used as biocatalysts for the biotransformation of (-)-${\alpha}$-pinene. After 96 hr, CER converted the bicyclic monoterpene hydrocarbon (-)-${\alpha}$-pinene into ${\alpha}$-terpineol (yield, 0.05 g/L), a monocyclic monoterpene alcohol, in addition to, other minor products. Using STH, verbenone was identified as the major biotransformed product, and minor products were myrtenol, camphor, and isopinocarveol. We did not observe any inhibitory effects of substrate or transformed products on mycelial growth of the fungi. The activities of fungal manganese-dependent peroxidase and laccase were monitored for 15 days to determine the enzymatic pathways related to the biotransformation of (-)-${\alpha}$-pinene. We concluded that a complex of enzymes, including intra- and extracellular enzymes, were involved in terpenoid biotransformation by white rot fungi.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1998.11a
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• pp.130-130
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• 1998

Two novel compounds SSC1 and SSC2 were isolated from culture broth produced from the strain of Stereum hirsutum by using of YM broth. They were isolated through HP-20 column chromatography, silica gel column chromatography and preparative HPLC, successively. The molecular formulas of SSC1 and SSC2. were determined as C$\sub$15/H$\sub$22/O$_3$ by high resolution EI -mass. The chemical structures of SSC1 and SSC2 were determined as sesquiterpenoid compounds by spectroscopic analysis of UV, IR, $^1$H NMR, $\^$13/C NMR, DEPT, HMQC and HMBC spectrum.

• The Korean Journal of Mycology
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• v.5 no.2
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• pp.17-20
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• 1977

Several fungi were collected at Mt. Sobaek, Andong and Euisung the period from 1974 to 1976. These fungi were identified and results are as followingj Lipophyllum fallax (Peck.) $K{\ddot{u}}hn$ et Romagn. Armillariella tabescens (Scop. ex Fr.) Sing., Crepidotus subphaerosporus (Lange) $K{\ddot{u}}hn$. et Romagn., Stereum hiugense Imaz., Coprinus radians (Desm.) Fr., Hygrophorus suzukaensis Hongo and Xylaria carpophila (Pers.) Fr. were listed newly by authors in Korea.

• Proceedings of the Zoological Society Korea Conference
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• 1999.10b
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• pp.60.2-60
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• 1999

No Abstract, See Full Text

• Journal of Microbiology and Biotechnology
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• v.22 no.11
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• pp.1540-1548
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• 2012

Manganese peroxidase (MnP) was isolated from the culture filtrate of the wood log mushroom Stereum ostrea (S. ostrea), grown on Koroljova medium, and then purified by ammonium sulfate [70% (w/v)] fractionation, DEAE-cellulose anion exchange chromatography, and Sephadex G-100 column chromatography, with an attainment of 88.6-fold purification and the recovery of 22.8% of initial activity. According to SDS-PAGE the molecular mass of the MnP was 40 kDa. The optimal pH and temperature were found to be 4.5 and $35^{\circ}C$, respectively. The enzyme was stable even after exposure to a pH range of 4.5 to 6.0, and at temperatures of up to $35^{\circ}C$ at a pH of 4.5 for 1h. The $K_m$ and $V_{max}$ values for the substrate phenol red were found to be $8{\mu}m$ and 111.14 U/mg of protein, respectively. The MnP also oxidized other substrates such as guaiacol, DMP, and veratryl alcohol. Sodium azide, EDTA, SDS, $Cu^{2+}$, and $Fe^{2+}$, at 1-5 mM, strongly inhibited enzyme activity, whereas $Ca^{2+}$ and $Zn^{2+}$ increased enzyme activity. The participation of the purified enzyme in the decolorization of dyes suggests that S. ostrea manganese peroxidase could be effectively employed in textile industries.

• Journal of Korea Foresty Energy
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• v.20 no.1
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• pp.6-11
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• 2001

In order to use bast fibers of mulberry tree at a pulp source of Hanji, the bast fibers were microbiologically treated with several wood rot fungi, and the microscopic characteristics of bast fibers depending on treatment days were evaluated. By wood rot fungi, Phanerochaete chrysosporium and Trametes versicolor, the weight reduction ratio was approximately 50 percent within incubation for 20 days. occurring together with decomposition of useful fibers. However, Hwterobasidion insularis and Stereum hirsutum have completely decomposed the utmost layer of black blue colored bast fibers, and not caused the damage if fibers. Until incubation for 10 days, the cellulose content of vast fibers by Stereum hirsutum was 78.9 percent with lignin content of 7.2 percent, showing an appropriate decomposition for useful fibers. By microscopic observation, the bundled fibers were separated to single fiber within treatement days 30 by Pleurotus ostreatus, and there were no damage on the surface of fiber by treatment days 50.

• Mycobiology
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• v.35 no.4
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• pp.210-214
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• 2007

Antibacterial and antifungal activities of liquid culture filtrate, water and ethanol extract (solid culture) of Stereum ostrea were evaluated against 5 bacteria and 3 plant pathogenic fungi. To determine the minimal inhibitory concentration (MIC), we studied $5{\sim}300\;mg/ml$ concentrations against bacteria and fungi separately. The MIC was 10 mg/ml for Bacillus subtilis and 40 mg/ml for Colletotrichum gloeosporioides and Colletotrichum miyabeanus. Liquid culture filtrate was more effective against Gram positive than Gram negative bacteria, and Staphylococcus aureus was the most inhibited (20.3 mm) bacterium. Water and ethanol extracts were effective against both Gram positive and Gram negative bacteria, and water extract was better than ethanol extract. In water and ethanol extract, inhibition zones were 23.6 and 21.0 mm (S. aureus) and 26.3 and 22.3 mm (Pseudomonas aeruginosa), respectively. For plant pathogenic fungi, the highest and lowest percent inhibition of mycelial growth (PIMG) was found 82.8 and 14.4 against C. miyabeanus and Botrytis cinerea in liquid culture filtrate, respectively. In water extract, the PIMG was found to be the highest 85.2 and lowest 41.7 for C. miyabeanus and C. gloeosporioides, respectively. The inhibitory effect of ethanol extract was better against C. miyabeanus than C. gloeosporioides and B. cinerea. Among 3 samples, water extract was the best against tested pathogenic fungi. This study offers that the extracts isolated from S. ostrea contain potential compounds which inhibit the growth of both bacteria and fungi.