• Radiation Oncology Journal
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• 제30권4호
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• pp.165-172
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• 2012

Purpose: To retrospectively evaluate the outcome and toxicity of total lymphoid irradiation (TLI) based conditioning regimen for allogeneic hematopoietic stem cell transplantation (HSCT) in severe aplastic anemia (SAA) patients who experienced an engraftment failure from prior HSCT or were heavily transfused. Materials and Methods: Between 1995 and 2006, 20 SAA patients received TLI for conditioning of HSCT. All patients were multi-transfused or had long duration of disease. Fifteen (75%) patients had graft failure from prior HSCT. In 18 (90%) patients, the donors were human leukocyte antigen identical siblings. The stem cell source was the peripheral blood stem cell in 15 (75%) patients. The conditioning regimen was composed of antithymocyte globulin plus TLI with a median dose of 750 cGy in 1 fraction. The graft-versus-host disease (GVHD) prophylaxis used cyclosporine with methotrexate. Results: With a median follow-up of 10.8 years, graft failures developed in 6 patients. Among them, 3 patients received their third HSCT to be engrafted finally. The Kaplan-Meier overall survival rate was 85.0% and 83.1% at 5 and 10 years, respectively. The incidence of acute and chronic GVHD was 20% and 20%, respectively. None of the patients have developed a malignancy after HSCT. Conclusion: In our study, TLI based conditioning in allogeneic HSCT was feasible with acceptable rates of GVHD in SAA patients who experienced graft failure from prior HSCT or was at a high risk of graft rejection. We achieved relatively better results of engraftment and survival with a long term follow-up.

• 대한설비공학회지:설비저널
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• 제3권3호
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• pp.213-220
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• 1974

• Molecules and Cells
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• 제41권3호
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• pp.207-213
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• 2018

Hypoxic culture is widely recognized as a method to efficiently expand human mesenchymal stem cells (MSCs) without loss of stem cell properties. However, the molecular basis of how hypoxia priming benefits MSC expansion remains unclear. In this report, our systemic quantitative proteomic and RT-PCR analyses revealed the involvement of hypoxic conditioning activated genes in the signaling process of the mitotic cell cycle. Introduction of screened two mitotic cyclins, CCNA2 and CCNB1, significantly extended the proliferation lifespan of MSCs in normoxic condition. Our results provide important molecular evidence that multipotency of human MSCs by hypoxic conditioning is determined by the mitotic cell cycle duration. Thus, the activation of mitotic cyclins could be a potential strategy to the application of stem cell therapy.

• Clinical and Experimental Pediatrics
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• 제50권7호
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• pp.613-621
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• 2007

Hematopoietic stem cell transplantation (HSCT) has expanded and evolved substantially in the last decades to treat various malignant and nonmalignant diseases. However, the conditioning regimen can lead to transplantation related death by major organ dysfunction, severe infection and bleeding. In the allogeneic setting, graft versus host disease may also develop, making post-transplant management complex. To overcome these problems, new stem cell sources, stem cell mobilizing agents and new skills, nonmyeloablative stem cell transplantation including reduced intensity stem cell transplantation has been introduced in clinical practice, but problems remained so far. Recipients of stem cell transplant may be severely immunocompromised for many months after transplantation. Furthermore, long-term complications (endocrine, metabolic, relapse, second malignancies, etc) can develop. Pediatrician is open called on to participate in the evaluation and consideration of patients for possible transplant and long-term follow-up of HSCT patients. This review is intended as a basic overview of HSCT relevant to general pediatrician.

• Journal of Biosystems Engineering
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• 제39권4호
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• pp.253-260
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• 2014

Purpose: This study investigated the harvesting properties of the giant miscanthus (Miscanthus ${\times}$ giganteus) to measure the required mowing power for different stem conditioning methods in order to shorten the drying time after mowing and the bale density so that the crop can be used as biomass in the winter season. Methods: The required mowing power and bale density were measured using a power measurement device, three different mower-conditioners, and a mid-sized round baler under different working speeds and conditioning methods. Results: For the various mower-conditioners, the average stem length from mowing was 0.86-0.91 m, and the available working speed was 1.6 m/s. The steel roller-type mower-conditioner showed better stem conditioning but could not mow over a working speed of 1.6 m/s. The required average power of the mower-conditioners varied from 23.8 kW for the steel roller-type rotary disk mower-conditioner with a working width of 2.4 m to 37.2 kW for the flail-type rotary disk mower-conditioner with a working width of 3.2 m at a working speed of 1.6 m/s. The bale densities were $155.8-172.2kg/m^3$. The highest bale density was measured for stems with no conditioning and a moisture content of 11.3% (d.b.) mowed by the rotary disk mower. The bale density was affected by the mowing method because of the low moisture content of the miscanthus stems. Conclusions: In terms of the working performance and conditioning statue, the steel roller-type mower-conditioner is a better choice at a working width of 2.4 m, while the flail finger-type mower-conditioner is better at a working width of 3.2 m. The type of mower-conditioner used for giant miscanthus harvesting should be determined by considering the harvest area, workable period, and working performance of a mower-conditioner and baler during the winter.

• Clinical and Experimental Pediatrics
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• 제52권6호
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• pp.725-729
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• 2009

중추신경계의 침범을 동반한 Epstein-Barr 바이러스 관련 혈구포식 림프조직구증은 조혈모세포이식을 시행하지 않으면 예후가 좋지 않다. 또한, 고식적인 강도의 전처치시 이식관련 사망률이 높다. 따라서 저자들은 진행성 중추신경계 침범을 보인 Epstein-Barr 바이러스 관련 혈구포식 림프조직구증 2례에서 저강도 전처치와 rituximab 후 타인 조혈모세포이식을 시행하여 치료하였기에 이를 보고하고자 한다.

• 설비공학논문집
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• 제19권12호
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• pp.874-882
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• 2007

The effects of a guide fin blade on the flow characteristics in a ventilating axial fan were investigated experimentally. The guide fins were setup onto the pressure surface of the blade, and their effects on the flowrate were evaluated. Two types of the guide fin blade were designed. One is the stem fin blade, and the other is the radial fin blade. The stem fin is designed normal to the circumference of a circle, and the radial fin is designed along the circumference of a circle. The results from the guide fin blade fans are compared with that of the blade without guide fins. The position and the geometry of the radial fin setting up on the blade have an effect on the increase of flowrate with the minor sacrifice of rotational speed of the blades. The radial fin positioning at 0.84 times blade diameter shows highest performance in the flowrate. The increase of the blade weight resulting from applying the guide fins shows minor effect on the variation of rotational speed of the blades.

• Biomolecules & Therapeutics
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• 제21권3호
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• pp.196-203
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• 2013

Recent accumulating studies have reported that hypoxic preconditioning during ex vivo expansion enhanced the self-renewal or differentiation of various stem cells and provide an important strategy for the adequate modulation of oxygen in culture conditions, which might increase the functional bioactivity of these cells for cardiac regeneration. In this study, we proposed a novel priming protocol to increase the functional bioactivity of cardiac progenitor cells (CPCs) for the treatment of cardiac regeneration. Firstly, patient-derived c-$kit^+$ CPCs isolated from the atrium of human hearts by enzymatic digestion and secondly, pivotal target molecules identified their differentiation into specific cell lineages. We observed that hCPCs, in response to hypoxia, strongly activated ERK phosphorylation in ex vivo culture conditioning. Interestingly, pre-treatment with an ERK inhibitor, U0126, significantly enhanced cellular proliferation and tubular formation capacities of CPCs. Furthermore, we observed that hCPCs efficiently maintained the expression of the c-kit, a typical stem cell marker of CPCs, under both hypoxic conditioning and ERK inhibition. We also show that hCPCs, after preconditioning of both hypoxic and ERK inhibition, are capable of differentiating into smooth muscle cells (SMCs) and cardiomyocytes (CMs), but not endothelial cells (ECs), as demonstrated by the strong expression of ${\alpha}$-SMA, Nkx2.5, and cTnT, respectively. From our results, we conclude that the functional bioactivity of patient-derived hCPCs and their ability to differentiate into SMCs and CMs can be efficiently increased under specifically defined culture conditions such as short-term hypoxic preconditioning and ERK inhibition.

• Clinical and Experimental Pediatrics
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• 제57권12호
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• pp.514-519
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• 2014

Hemorrhagic cystitis is a common stem cell transplantation-related complication. The incidence of early-onset hemorrhagic cystitis, which is related to the pretransplant conditioning regimen, has decreased with the concomitant use of mesna and hyperhydration. However, late-onset hemorrhagic cystitis, which is usually caused by the BK virus, continues to develop. Although the BK virus is the most common pathogenic microorganism of poststem cell transplantation late-onset hemorrhagic cystitis, pediatricians outside the hemato-oncology and nephrology specialties tend to be unfamiliar with hemorrhagic cystitis and the BK virus. Moreover, no standard guidelines for the early diagnosis and treatment of BK virus-associated hemorrhagic cystitis after stem cell transplantation have been established. Here, we briefly introduce poststem cell transplantation BK virus-associated hemorrhagic cystitis.

• Radiation Oncology Journal
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• 제32권3호
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• pp.198-207
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• 2014

Purpose: To evaluate the effects of total body irradiation (TBI), as a conditioning regimen prior to allogeneic stem cell transplantation (allo-SCT), in pediatric acute leukemia patients. Materials and Methods: From January 2001 to December 2011, 28 patients, aged less than 18 years, were treated with TBI-based conditioning for allo-SCT in our institution. Of the 28 patients, 21 patients were diagnosed with acute lymphoblastic leukemia (ALL, 75%) and 7 were diagnosed with acute myeloid leukemia (AML, 25%). TBI was completed 4 days or 1 day before stem cell infusion. Patients underwent radiation therapy with bilateral parallel opposing fields and 6-MV X-rays. The Kaplan-Meier method was used to calculate survival outcomes. Results: The 2-year event-free survival and overall survival rates were 66% and 56%, respectively (71.4% and 60.0% in AML patients vs. 64.3% and 52.4% in ALL patients, respectively). Treatment related mortality rate were 25%. Acute and chronic graft-versus-host disease was a major complication; other complications included endocrine dysfunction and pulmonary complications. Common complications from TBI were nausea (89%) and cataracts (7.1%). Conclusion: The efficacy and toxicity data in this study of TBI-based conditioning to pediatric acute leukemia patients were comparable with previous studies. However, clinicians need to focus on the acute and chronic complications related to allo-SCT.