• Title/Summary/Keyword: Stable feeding

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Determination of Biosynthetic Pathway of Decursin in Hairy Root Culture of Angelica gigas

  • Ji, Xiuhong;Huh, Bum;Kim, Soo-Un
    • Applied Biological Chemistry
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    • v.51 no.4
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    • pp.258-262
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    • 2008
  • To establish the biosynthetic pathway of decursin in Angelica gigas Nakai, feeding experiment with stable isotope-labeled precursors were conducted. Umbelliferone and decursin were labeled with deuterium at C-3. The umbelliferone, the decursin, and other commercially available putative precursors, L-phenylalanine-ring-$d_5$ and trans-cinnamic acid-$d_7$, were fed to the hairy root culture of A. gigas. Each deuterated compound was incorporated into decursinol, decursinol angelate, and decursin as determined by mass spectrometric analysis. These findings confirmed the coumarin biosynthesis pathway sequence is composed of phenylalanine, cinnamic acid, umbelliferone, decursinol, and decursin.

Preparation of $\gamma-LiAlO_2$ Short Fibers by the Sol-Gel Method (졸-겔법에 의한 $\gamma-LiAlO_2$ 단섬유의 제조)

  • 현상훈;이재현;홍성안
    • Journal of the Korean Ceramic Society
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    • v.33 no.10
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    • pp.1079-1088
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    • 1996
  • Discontinuous ${\gamma}$-LiAlO2 fibers for fiber-reinforcing MCFC matrixes have been produced by the sol-gel process using the centrifugal spinning apparatus of the Rotary type. Gel fibers could be obtained through spinning of stable LiAlO2 complex polymetric sols under the optimum spinning conditions (hollow-disc rotating velocity 9000 rpm sol feeding rate of 4ml/min flowing N2 temperature of 4$0^{\circ}C$ and flowing N2 pressure of 4 bar). It was found that defect free and densified ${\gamma}$-LiAlO2 fibers with the relative density of 98% and the mean diameter of 4.7${\mu}{\textrm}{m}$ were prepared when the spinned fibers were heat-treated to 100$0^{\circ}C$ on the specified heating schedule. in particular the mean diameter and length of fibers could be controlled by the pressure of flowing N2 and the chopping-sieving method respectively.

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A neural network method for recognition of part orientation in a bowl feeder (보울 피이더에서 신경 회로망을 이용한 부품 자세 인식에 관한 연구)

  • 임태균;김종형;조형석;김성권
    • 제어로봇시스템학회:학술대회논문집
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    • 1990.10a
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    • pp.275-280
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    • 1990
  • A neural network method is applied for recognizing the orientation o f individual parts being fed from a bowl feeder. The system is designed in such a way that a part can be discriminated and sorting according to every possible stable orientation without implementing any a mechanical tooling. The operation of the bowl feeder is based on a 2D image obtained from an array of fiber optic sensor located on the feeder track. The acquired binary image of a moving and vibrating part is used as input to a neural network which, in turn, determines t he orientation of the part. The main task of the neural network, here is to synthesize the appropriate internal discriminant functions for the part orientation using the part features. A series of the experiments reveals several promising points on performance. Since the operation of the feeder is highly programmable, it is well suited for feeding and sorting small parts prior to small batch assembly work.

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Studies of Repeated Fed-Batch Fermentation of Cephalosporin C in an Immobilized Cell Bioreactor

  • Park, Hong-Je;Khang, Yong-Ho
    • Journal of Microbiology and Biotechnology
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    • v.5 no.4
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    • pp.229-233
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    • 1995
  • Acremonium chrysogenum was immobilized in ionotropic gel beads to develop semi-continuous production of cephalosporin C (CPC). Barium alginate beads were more stable than calcium alginate or strontium alginate beads in chemically defined media. The gel stability of Ba-alginate was further increased by cross-linking with polyethyleneimine (PEI). The presence of carboxymethyl cellulose inside Ba-alginate beads did not reduce mass transfer resistance. Ba-alginate microbeads that had little diffusion limitation increased CPC production rate 1.6 fold higher than that of normal beads. CPC fermentation with immobilized cells in Ba-alginate microbeads was performed continuously for 40 days by way of repeated fed-batch operations. Mathematical modeling was developed to describe the repeated fed-batch fermentation system. Results of the computer simulation agreed well with the experimental data, which made it possible to predict an optimal feeding rate that could maximize total CPC productions.

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Antimicrobial effect of chitosan oligosaccharides, prepared under ultrafiltration membrane bioreactor, against pathogenic bacteria causing flounder fish diseases in aquacultural farm

  • Heo, Moon-Soo;Jeon, You-Jin;Lee, Ki-Wan;Song, Choon-Bok;Lee, Jehee;Yeo, In-Kyo;Yang, Byung-Gyoo;Kim, Se-Kwon
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2001.10a
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    • pp.145-146
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    • 2001
  • Despite a variety of development in fish farming during the last decades, fish diseases by bacteria, virus, and parasites are still major problems in aquaculture. Aquaculture of Hounder fish is widely performed around Korea as well as Jeju island, due to relatively stable seed production, short farming period, and a higher value in market. However, intensive feeding and environmental pollution in aquacutural farm act as a great limiting factor in economic aspect. (omitted)

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THE NEW MULTI-OUTPUT DC-DC CONVERTER USING THE CURRENT-FED CONVERTER (전류환류형 DC-DC 콘버터를 이용한 새로운 다출력회로)

  • Ahn, T.Y.;Lee, Y.J.;Kim, H.J.
    • Proceedings of the KIEE Conference
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    • 1988.07a
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    • pp.144-147
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    • 1988
  • The current-fed DC-DC converter, which is known as the most stable DC-DC converter, has a two-winding reactor in series with the input. In this paper the new multi-output DC-DC converter circuit, in which the 2nd winding of the reactor is creating the 2nd output, while the 2nd winding is feeding the energy to input in the current-fed converter, is proposed. The steady state characteristics of the new circuit are clarified. And it is found that the maximum value exists in the 2nd output.

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The Double-Output DC-DC Converter Using the Current-Fed Converter (전류환류형 DC-DC콘버터를 이용한 이중출력 회로)

  • 이윤종;김희준;안태영
    • The Transactions of the Korean Institute of Electrical Engineers
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    • v.38 no.6
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    • pp.451-457
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    • 1989
  • The current-fed DC-DC converter, which is known as the most stable DC-DC converter, has a two-winding reactor in series with the input. In this paper a new double-output DC-DC converter circuit, in which the 2nd winding of the reactor is creating the 2nd output, while the 2nd winding is feeding the energy to the input in the current-fed converter, is propose. The steady state characteristics of the new circuit are clarified and it is found that the maximum value exists in the 2nd output. Furthermore, regulation characteristic is analysed by 'Slope method' and the result shows good agreement with experimental value. The 2nd output voltage regulation is performed by using regulation IC. As a result, we have achieved good regulation characteristics.

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Food Sources of the Ascidian Styela clava Cultured in Suspension in Jindong Bay of Korea as Determined by C and N Stable Isotopes (탄소 및 질소안정동위원소 조성에 의한 남해안 진동만 양식 미더덕의 먹이원 평가)

  • Moon, Changho;Park, Hyun Je;Yun, Sung Gyu;Kwak, Jung Hyun
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.19 no.4
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    • pp.302-307
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    • 2014
  • To examine the trophic ecology of the ascidian Styela clava in an aquaculture system of Korea, stable carbon and nitrogen isotopes were analyzed monthly in S. clava, coarse ($>20{\mu}m$, CPOM) and fine particulate organic matters ($0.7<<20{\mu}m$, FPOM). CPOM (means: $-18.5{\pm}1.2$‰, $9.3{\pm}0.7$‰) were significantly higher ${\delta}^{13}C$ and ${\delta}^{15}N$ values than those ($-20.5{\pm}1.5$‰, $8.4{\pm}0.5$‰) of FPOM. S. clava had mean ${\delta}^{13}C$ and ${\delta}^{15}N$ values of $-18.9({\pm}1.7)$‰ and $11.6({\pm}0.7)$‰, respectively. S. clava were more similar to seasonal variations in ${\delta}^{13}C$ and ${\delta}^{15}N$ values of FPOM than those of CPOM, suggesting that they rely largely on the FPOM as a dietary source. In addition, our results displayed that the relative importance between CPOM and FPOM as dietary source for the ascidians can be changed according to the availability of each component in ambient environment, probably reflecting their feeding plasticity due to non-selective feeding irrespective of particle size. Finally, our results suggest that dynamics of pico- and nano-size plankton (i.e., FPOM) as an available nutritional source to S. clava should be effectively assessed to maintain and manage their sustainable aquaculture production.

Trophic Level and Ecological Niche Assessment of Two Sympatric Freshwater Fish, Microphysogobio rapidus and Microphysogobio yaluensis Using Stable Isotope Analysis (안정동위원소 분석을 활용한 멸종위기종 여울마자와 동서종 돌마자의 영양단계 및 생태적 지위 평가)

  • Dae-Hee Lee;Hye-Ji Oh;Yerim Choi;Geun-Hyeok Hong;InHyuck Baek;Keun-Sik Kim;Kwang-Hyeon Chang;Ju-Duk Yoon
    • Korean Journal of Ecology and Environment
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    • v.57 no.1
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    • pp.39-50
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    • 2024
  • In ecosystems within limited resources, interspecific competition is inevitable, often leading to the competitive exclusion of inferior species. This study aims to provide foundational information for the conservation and restoration management of Microphysogobio rapidus by evaluating species' ecological response to biological factors within its habitat. To understand this relationship, we collected food web organisms from site where M. rapidus coexist with Microphysogobio yaluensis, a specie ecologically similar to M. rapidus, and evaluated the trophic levels (TL), isotopic niche space (INS), and the overlap of INS among fishes within the habitat using stable isotope analysis. Our analysis revealed that the M. rapidus exhibited a higher TL than M. yaluensis, with TL of 2.6 and 2.4, respectively. M. yaluensis exhibited a broad INS, significantly influencing the feeding characteristics of most fish. Conversely, M. rapidus showed a narrow INS and asymmetric feeding relationships with other species, in habitats with high competition levels. This feeding characteristics of M. rapidus indicate that the increase in competitors sharing the similar resources lead to a decrease in available resources and, consequently, is expected to result in a decrease in their density.

Study on the Optimized Condition of the Slot-Die Coating Process (슬롯다이 코팅의 최적화 조건에 관한 연구)

  • Seo, Eung Soo;Ye, Jungwoo;Hwang, Joong Kook;Shim, Jaesool;Chai, Young Suck
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.39 no.9
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    • pp.937-945
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    • 2015
  • In this paper, we study the coating performance according to the slot-die conditions in the coating process. The quality of the slot-die coating depends on factors such as feeding speed of film, the viscosity of liquid, and the pressure applied to the slot-die. In this study, we determine the optimum conditions for a stable coating by performing 2-D and 3-D simulations. We carry out numerical simulations with respect to the feeding velocity of the film, the pressure of the slot-die inlet, and the viscosity of the coating liquid. Based on the results, the coating was the most reliable when the pressure of the inlet was $5kgf/cm^2$, the viscosity was about 100 cps and the velocity was 20 m/min.