• FLOWER RESEARCH JOURNAL
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• v.18 no.3
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• pp.211-215
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• 2010

A new spray chrysanthemum(Dendranthema grandiflorum) cultivar 'Magic' was developed from a cross combination between 'Artist Pink' and 'SP0347' by selection of seedlings and lines at the Flower Research Institute, Gyeongnam Agricultural Research and Extension Services(ARES) from 2003 to 2007. Its characteristics were investigated three times from 2005 to 2007 under condition of forcing culture in spring and retarding culture in autumn. The natural flowering time of 'Magic' was October 26th, and year-round production was possible by day length treatment. Its capitulum was 6.1 cm in diameter, and had 31.9 ray florets and 11.7 head per stem in autumn. Its ray floret was white-purple with yellow green central zone. Days to flower of 'Magic' was about 43 days in spring, and 'Magic' showed the vase life of 20.6 days in autumn. This cultivar was registered for a commercialization in 2007.

• FLOWER RESEARCH JOURNAL
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• v.18 no.4
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• pp.238-243
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• 2010

This study was conducted to investigate effects of nutrient solution pH control agent and foliar spray of ethephon on the reduction of stem inside-cavity and improvement of flower quality in chrysanthemum 'Baekma'. Changes of pH in nutrient solution as affected by the kind of pH control agent showed more settled pattern in the plot of KOH treatment than in others. Plant growth and development such as cut flower length, leaf numbers, cut flower weight and petal number were the greatest in the plot of KOH. However, the size and area of stem cavity was large in KOH rather than $KHCO_3$. As changes in the absorbing patterns of mineral elements as affected by nutrient solution pH control agent in closed system, contents of total nitrate, phosphorus, potassium and calcium by $KHCO_3$ was absorbed into the plant less than KOH, so that it remained a lot of mineral element residues rather than KOH in closed system. Plant growth as affected by the foliar spray of ethephon showed growth retardation effect in the plot of solution diluted to 1 : 500 and growth promotion effect in the plot of solution diluted to 1 : 1,000 or 1 : 2,000. The number of petals was the best in the plot of foliar spray of ethephon solution diluted to 1 : 1,000 before flowering at 45 days showing 331 petals compared to control showing 302 petals. The size and area of stem cavity as affected the foliar spray of ethephon was smaller 1 mm and 7%, respectively, in the plot of solution diluted to 1 : 2,000 before flowering at 30 days than in control. Therefore, treating pH stabilization using KOH after floral initiation stage with the foliar spray of ethephon solution diluted to 1 : 2,000 before flowering at 30 days would help to reduce stem cavity size and improve flower quality in hydroponically grown chrysanthemum 'Baekma'.

• Horticultural Science & Technology
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• v.31 no.5
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• pp.544-551
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• 2013

The effect of different silicon (Si) sources and methods of application on the growth of two chrysanthemum cultivars grown in a soilless substrate was investigated. Rooted terminal cuttings of Dendranthema grandiflorum 'Lemmon Eye' and 'Pink Eye' were transplanted into pots containing a coir-based substrate. A nutrient solution containing 0 or $50mg{\cdot}L^{-1}$ Si from calcium silicate ($CaSiO_3$), potassium silicate ($K_2SiO_3$) or sodium silicate ($Na_2SiO_3$) was supplied once a day through an ebb-and-flood sub irrigation system. A foliar spray of 0 or $50mg{\cdot}L^{-1}$ Si was applied twice a week. Cultivar and application method had a significant effect on plant height. Cultivar, application method, and Si source had a significant effect on plant width. Of the three Si sources studied, $K_2SiO_3$ was found to be the best for the increasing number of flowers, followed by $CaSiO_3$ and $Na_2SiO_3$. In both the cultivars, sub irrigational supply of Si developed necrotic lesions in the older leaves at the beginning of the flowering stage as compared to the control and foliar spray of Si. Cultivar, application method, Si source, and their interactions had significant influence on leaf tissue concentrations of calcium (Ca), potassium (K), phosphorus (P), magnesium (Mg), sulfur (S), sodium (Na), boron (B), iron (Fe), and zinc (Zn). The addition of Si to the nutrient solution decreased leaf tissue concentrations of Ca, Mg, S, Na, B, Cu, Fe, and Mn in both cultivars. The greatest Si concentration in leaf tissue was found in 'Lemmon Eye' ($1420{\mu}g{\cdot}g^{-1}$) and 'Pink Eye' ($1683{\mu}g{\cdot}g^{-1}$) when $K_2SiO_3$ was applied through a sub irrigation system and by foliar spray, respectively.

• FLOWER RESEARCH JOURNAL
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• v.16 no.3
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• pp.200-204
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• 2008

A new spary chrysanthemum(Dendranthema grandiflorum) cultivar 'Serapina' was developed from a cross between 'Relanse' and 'Yen' by selection of seedlings and lines at the Flower Research Institute, Gyeongnam Agricultural Research and Extention Services(ARES) from 2002 to 2006. Its characteristics were investigated three times from 2004 to 2006 under condition of forcing culture in spring and retarding culture in autumn. The natural flowering time of 'Serapina' was October 23th, and year-round production was possible by day length treatment. Its capitulum was 2.8cm in diameter, and had 43.4 ray florets and 28.9 head per stem in autumn. Its ray floret was yellow with green central zone. To flower in the short day condition, for 'Serapina' was about 46 days in spring, and 'Serapina' showed the vase life of 21.7 days in autumn. This cultivar was registered for a commercialization in 2007.

• Korean Journal of Breeding Science
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• v.40 no.4
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• pp.490-494
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• 2008

A new spary chrysanthemum (Dendranthema grandiflorum) cultivar 'Eye Ball' was developed from a cross between 'Rima' and 'Yen' followed by selections of seedlings and lines at the Flower Rearch Institute, Gyeongnam Agricultural Research and Extension Services (ARES) from 2002 to 2006. Its characteristics were investigated three times from 2004 to 2006 under forcing culture in spring and retarding culture in autumn. The natural flowering time of 'Eye Ball' was October 20th, but year-round production was possible by shading and lighting. Its flower was 3.1cm in diameter, and had 38.4 petals and 26.8 flowers per stem in autumn. Its petal was narrow (0.4 cm) and pink with green in color at the flower center. To flower in the short day condition, for 'Eye Ball' was about 47 days in spring, and 'Eye Ball' showed a vase life of 20.9 days in autumn. This cultivar was registered for a commercialization in 2007. 'Eye Ball' would be cultured under the greenhouse and rain shelter condition in Korea.

• Korean Journal of Breeding Science
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• v.40 no.2
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• pp.159-163
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• 2008

A New spary chrysanthemum (Dendranthema grandiflorum) cultivar 'Water Fog' was bred by open pollination of 'Angaesoguk'. Its characteristics were investigated three times from 2004 to 2006 under forcing culture in spring and retarding culture in autumn. The natural flowering time of 'Water Fog' was 24 October, but year-round production was possible by shading and lighting. It had flower diameter of 2.3 cm, 46.5 petals and 34.4 flowers per stem in autumn. The petal was narrow(0.4cm) and the color of petal was white with presence of green at flower center. Days to flower from short-day start for 'Water Fog' was about 48 in spring. 'Water Fog' showed the vase life of 22.6 days in autumn. This cultivar was registered for a commercialization in 2007. 'Water Fog' would be cultured under greenhouse and rain shelter condition in Korea.

• Horticultural Science & Technology
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• v.28 no.3
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• pp.449-455
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• 2010

'Moulinrouge' was selected as the best regenerating cultivar among 18 different spray-type chrysanthemum cultivars bred in the Gyeongnam Flowers Breeding Research Institute. When the leaf explants from standard- and spray-type chrysanthemum 'Jinba' and 'Moulinrouge' were incubated on MS basal medium supplemented with $0.5mg{\cdot}L^{-1}$ BA and $1.0mg{\cdot}L^{-1}$ NAA, both 'Jinba' and 'Moulinrouge' induced adventitious shoots that can be regenerated into plantlets. Based on these regeneration conditions, we developed an efficient $Agrobacterium$-mediated chrysanthemum 'Moulinrouge' transformation method by using sequential selection of shoots from low ($10mg{\cdot}L^{-1}$) to high ($30mg{\cdot}L^{-1}$) concentrations of kanamycin after co-cultivation of leaf explants with $Agrobacterium$ for 10 days and induction of shoots. All kanamycin resistant plants investigated with genomic PCR analysis carried the report gene, $AtSICKLE$, in their genome. Although expression levels of the report gene in the transgenic plants investigated with RT-PCR were relatively low because of inefficiency of CaMV 35S promoter in chrysanthemum, transgenic lines expressing $AtSICKLE$ efficiently showed leaf epinasty phenotype. We expect that our results will provide a useful method that can perform a high-throughput investigation of genes isolated and studied well in model plants for molecular breeding of chrysanthemum.

• Journal of Plant Biotechnology
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• v.42 no.4
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• pp.370-375
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• 2015

This experiment compared the regeneration conditions of the radiation mutant spray chrysanthemum 'purple ND'. The four different flower blooming stages (S1: 10% opened flower, S2: 30% opened flower, S3: 50% opened flower, and S4: 70% opened flower) and different petal parts (TBOP: the basal of petal and TEOP: the end of petal) were used to compare regeneration conditions between plants grown in MS medium supplemented with IAA and BAP. The highest adventitious shooting rate was identified in plants grown on the IAA $1.0mg{\cdot}L^{-1}$ and BAP $2.0mg{\cdot}L^{-1}$ when using the end of petal at the S2 stage. It displayed 79.2% regeneration and produced 33.4 shoots. Rooted plantlets were successfully established in the greenhouse, showing the same morphological characteristics of vegetative and reproductive organs with those of the mother plant. Flow cytometry analysis revealed no ploidy variation between the regenerated plants and the mother plant grown under greenhouse conditions.

• Korean Journal of Breeding Science
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• v.42 no.6
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• pp.606-610
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• 2010

A new cultivars Dendranthema grandiflourm 'Dream Moon' was developed at Gyeonggi-do Agricultural Research and Extension Services (GARES), Korea in 2008. The cultivar 'Dream Moon' was derived from a cross between 'Patra', a spray cultivar with yellow single type, and 'Sei-rosa', a spray cultivar with pink single type in 2005. The cultivar has single type flowers with pink petals. Trial evaluation was conducted from 2006 to 2008 for a shading cultivation in summer and a retarding cultivation in spring. The flowering time of 'Dream Moon' is late October, and year-round flowering is possible by shade or light treatment. The diameter of flower is 56.0 mm. Numbers of flowers per stem and petals per flower are 16.4 and 24.6, respectively. After investing of specific characters from 2006 to 2008, it was finally selected and named 'Dream Moon'. It has resistance to white rust and the vase life is about 20.7days in autumn season.

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 2005.05a
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• pp.100-104
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• 2005