• Korean Journal of Pharmacognosy
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• v.18 no.2
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• pp.107-111
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• 1987

3-O-acetyl pomolic acid methyl ester and p-coumaric acid methyl ester were isolated from the root of Spiraea prunifolia var. simpliciflora $N_{AKAI}$ and their structures were identified on the basis of spectral data.

• Korean Journal of Plant Taxonomy
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• v.53 no.1
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• pp.32-37
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• 2023

Spiraea prunifolia f. simpliciflora Nakai is a perennial shrub widely used for horticultural and medicinal purposes. We simultaneously obtained the complete plastid genome (plastome) and nuclear ribosomal gene transcription units, 45S nuclear ribosomal DNA (nrDNA) and 5S nrDNA of S. prunifolia f. simpliciflora, using Illumina short-read data. The plastome is 155,984 bp in length with a canonical quadripartite structure consisting of 84,417 bp of a large single-copy region, 18,887 bp of a short single-copy region, and 26,340 bp of two inverted repeat regions. Overall, a total of 113 genes (79 protein-coding genes, 30 tRNAs, and four rRNAs) were annotated in the plastome. The 45S nrDNA transcription unit is 5,848 bp in length: 1,809 bp, 161 bp, and 3,397 bp for 18S, 5.8S, and 26S, respectively, and 261 bp and 220 bp for internal transcribed spacer (ITS) 1 and ITS 2 regions, respectively. The 5S nrDNA unit is 512 bp, including 121 bp of 5S rRNA and 391 bp of intergenic spacer regions. Phylogenetic analyses showed that the genus Spiraea was monophyletic and sister to the clade of Sibiraea angustata, Petrophytum caespitosum and Kelseya uniflora. Within the genus Spiraea, the sections Calospira and Spiraea were monophyletic, but the sect. Glomerati was nested within the sect. Chamaedryon. In the sect. Glomerati, S. prunifolia f. simpliciflora formed a subclade with S. media, and the subclade was sister to S. thunbergii and S. mongolica. The close relationship between S. prunifolia f. simpliciflora and S. media was also supported by the nrDNA phylogeny, indicating that the plastome and nrDNA sequences assembled in this study belong to the genus Spiraea. The newly reported complete plastome and nrDNA transcription unit sequences of S. prunifolia f. simpliciflora provide useful information for further phylogenetic and evolutionary studies of the genus Spiraea, as well as the family Rosaceae.

• Korean Journal of Pharmacognosy
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• v.27 no.4
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• pp.389-396
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• 1996

Spiraea prunifolia var. simpliciflora (Rosaceae) is a deciduous. latifoliate shrub growing in most parts of Korea. The roots of this plant have been used for malaria, as antipyretics and emetics. From the roots of this plant, sterol glycoside and two triterpenoids were isolated and the structures were elucidated by chemical and spectroscopic methods. They were identified as $3{\beta}-hydroxyurs-12-ene-28-oic$ acid (ursolic acid.1), $2{\alpha}$, $3{\beta}$, $19{\alpha}-trihy-droxyurs-12-ene-28-oic$ acid (tormentic acid.2) and ${\beta}-sitoste-rol-3-O-{\beta}-D-glucopyranoside$ (3).

• Korean Journal of Pharmacognosy
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• v.43 no.3
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• pp.193-197
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• 2012

Korean folk medicine 'JoPabNaMu' has been mainly used to cure removal of fever and neuralgia. With regard to the botanical origin of 'JoPabNaMu', it has been considered to designate the stem of Spiraea species (Rosaceae), but there was no pharmacognostical confirmation on it. To clarify the botanical origin of 'JoPabNaMu', the anatomical characteristics of the branch of Spiraea species growing wild in Korea were studied. As a result, 'JoPabNaMu' was proved to be the branch of Spiraea prunifolia var. simpliciflora.

• Korean Journal of Pharmacognosy
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• v.13 no.1
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• pp.39-42
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• 1982

Spiraea prunifolia Sieb. et. Zucc. var. simpliciflora Nakai (Rosaceae) is distributed in Korea, and used as a folk medicine for antipyretic, antimalarial and emetic. Sterols were obtained from the methanolic extract of the root of above plant. The composition of sterols are campesterol and ${\beta}-sitosterol$ which has been determined by gaschromatographic analysis.

• Journal of Life Science
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• v.19 no.9
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• pp.1183-1189
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• 2009

85 individual Spiraea prunifolia for. simpliciflora (Rosaceae) were sampled to examine the genetic diversity and population structure of S. prunifolia for. simpliciflora populations. Inter-simple sequence repeats (ISSR) produced 65 polymorphic loci and identified 78 ISSR genotypes. Three multilocus genotypes were shared by more than one plant within a population. Total genetic diversity values ($H_T$) and inter-locus variation in the within-population genetic diversity ($H_S$) were 0.293 and 0.183, respectively. On a per-locus basis, the proportion of total genetic variation due to differences among populations ($G_{ST}$) was 0.373. This indicated that about 37.3% of the total variation was among populations. ISSR markers are very effective in classifying natural population levels of S. prunifolia for. simpliciflora in Korea. In addition, insights into the relative gene diversity among and within populations of S. prunifolia for. simpliciflora would be useful in plant breeding and also for the development of strategies for ex situ conservation of plant genetic resources.

• Journal of Applied Biological Chemistry
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• v.61 no.4
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• pp.397-403
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• 2018

The leaves of Spiraea prunifolia were extracted with 80% aqueous MeOH and the concentrates were partitioned into EtOAc, n-BuOH, and $H_2O$ fractions. The repeated $SiO_2$ or ODS column, and medium pressure liquid chromatographies for the n-BuOH fraction led to isolation of two phenolic glucosides. The chemical structures of these compounds were determined as isosalicin (1) and crenatin (2) based on spectroscopic analyses including Nuclear magnetic resonance and MS. Extracts were analyzed using UPLC-MS/MS providing a short analysis time within 5 min using MRM technique. The concentration of crenatin was higher as 9.53 mg/g and isosalicin was lower as 0.65 mg/g. Neuroprotective effects of these compounds against hydrogen peroxide ($H_2O_2$)-induced neurotoxicity were evaluated. The results showed that exposure to $H_2O_2$ induced morphological changes, cell death and neurotoxicity in SK-N-MC cells. However, pretreatment with crenatin resulted in inhibition of morphological change, reduction of loss of cell viability and attenuation of neuronal damage. These results suggested that neuroprotective effect of crenatin isolated from S. prunifolia can be a good candidate for the development of health beneficial foods which can ameliorate the degenerative neuronal disease caused by oxidative stress.

• The Korean Journal of Mycology
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• v.33 no.1
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• pp.35-37
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• 2005

Anthracnose symptoms on Spiraea prunifolia were observed around the residential area of Dongbumyon, Geoje City, Gyeongnam Province, Korea in 2002 and 2003. Typical symptoms of the disease were dark red spot on the leaves and black spot on the stem. The pathogen formed gray to dark gray colony on potato dextrose agar. Conidia were single celled, color, ovoid or oblong in shape and $6{\sim}12\;{\times}\;3{\sim}5\;{\mu}m$ in size. Seta were dark brown in color, cone-shaped in shape and $23{\sim}42\;{\times}\;2{\sim}4\;{\mu}m$ in size. The optimum temperature for growth of the isolate was about $25{\sim}30^{\circ}C$. On the basis of mycological characteristics and pathogenicity test on host plants, the fungus was identified as Colletotrichum gloeosporioides. This is the first report on the anthracnose of S. prunifolia caused by C. gloeosporioides in Korea.

• The Plant Pathology Journal
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• v.15 no.1
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• pp.76-78
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• 1999

A Phloeospora leaf spot disease on Spiraea prunifolia var. simpliciflora was noticed to commonly occur in Korea. The causal organism of the disease was identified as Phloeospora spiraeicola based on the morphological characteristics of conidiomata and conidia. Pathogenicity of the fungus was proved by artificial inoculation. This is the first record of Phloeospora leaf spot in Korea.

• Journal of the Korean Institute of Landscape Architecture
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• v.26 no.2
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• pp.91-100
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• 1998

This paper is to study on rating system of some shrubs for pedestrian control with concentrate upon the density of branch. It was usd that Hibiscus syricacus L., Spiraea prunifolia var. simpliciflora Nak, Ligustrum obtusifolium S. et Z., Callicarpa dichotoma Raeusch., Rhoododendron mucrionulatum Turcz., Syringa vulgaris L., Weigela subsessilis L. H. Bailey, Cercis chinensis bunge, Forsythia koreana Nak., Euonymus alatus Sieb, Chaenomeles speciosa Nak., orbaria sorbifolia var. stellipila Max., Deutzia parviflora Bunge, Kerria japonica De Candolle, Prunus tomentosa Thunberg ex Murray, Purunus grandulosa for. albiplena Koehne. Shrubs are invesitgated ito the density of branch, the power of sprout, height, a rate of growth, hardness of naturalizaton, crown width and existence of thorns. Shrubs belonged to high group of rating system for pedestrian control were Euonymus alatus Sieb, Purunus grandulosa for. albiplena Koehne, Chaenomeles speciosa Nak., Spiraea prunifolia var. simpliciflora Nak., Prunus tomentosa Thunberg ex Murray, Rhododendron mucronulatum Turcz., Hibiscus syricacus L., Ligustrum obtusifolium S. et Z., Syringa vulgaris L., Weigela subsessilis L.H.Bailey.