• Fisheries and Aquatic Sciences
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• v.20 no.7
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• pp.15.1-15.7
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• 2017

An 8-week feeding trial was conducted to study the effect of dietary supplementation of a biofloc powder on growth performance and non-specific immune response of Litopenaeus vannamei. Seven experimental diets were prepared with supplementation of graded levels of dried biofloc powder by 0, 0.5, 1.0, 2.0, 4.0, 6.0, and 8.0% (designated as Con, BF0.5, BF1, BF2, BF4, BF6, and BF8, respectively). Triplicate groups of shrimp ($1.01{\pm}0.01g$) were hand-fed with one of the diets four times a day. At the end of the feeding trial, significantly (P<0.05) higher growth performance and feed utilization were obtained in BF4 groups compared to those fed the Con diet. The innate immunity of shrimp was improved by the dietary supplementation of biofloc. Dietary inclusion of biofloc at the level of 4.0% significantly increased disease resistance of shrimp against Vibrio harveyi. The results indicate that biofloc might be used as a dietary supplement for growth performance, innate immunity and disease resistance of Pacific white shrimp.

• Journal of fish pathology
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• v.23 no.3
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• pp.273-280
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• 2010

To determine whether rock bream Oplegnathus fasciatus can be protected from rock bream iridovirus (RBIV) infection by intramuscular injection of long double-stranded RNAs (dsRNAs), we compared protective effect of virus-specific dsRNAs corresponding to major capsid protein (MCP), ORF 084, ORF 086 genes, and virus non-specific green fluorescent protein (GFP) gene. Furthermore, to determine whether the non-specific type I interferon (IFN) response was associated with protective effect, we estimated the activation of type I IFN response in fish using expression level of IFN inducible Mx gene as a marker. As a result, mortality of fish injected with dsRNAs and challenged with RBIV was delayed for a few days when comparing with PBS injected control group. However, virus-specific dsRNA injected groups exhibited no significant differences in survival period when compared to the GFP dsRNA injected group. Semi-quantitative analysis indicated that the degree of antiviral response via type I IFN response is supposedly equal among dsRNA injected fish. These results suggest that type I IFN response rather than sequence-specific RNA interference might involve in the lengthened survival period of fish injected with virus-specific dsRNAs.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.101.1-101
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• 2003

Lipopolysaccharide, siderophore, and cyclic dipeptide have been shown to be necessary for ISR induction by pseudomnads. However, there is no report on cloning of genes or generating specific mutants involving in ISR activity. A biological control bacteium P. chlororaphis O6 induces resistance to Erwinia carotovora subsp. carotovara SCCI in tobacco and induces drought resistance in Arabidopsis. To isolate genes involved in ISR activity and induction of drough resistance of O6, we constructed Tn5 mutants and were used to screen for ISR activity and drought resistance activity using microtiter assay with tobacco and Arabidopsis. Thirty-three ISR-deficient mutants were selected, and the nine ISR-deficient mutants were also lost activity of drought resistance. The flanking sequence analysis of the ISR and drought resistance-deficient mutants showed that a gacS gene encoding a two-component sensor kinase, and a mce gene encoding a protein involved in mycobacterial cell entry were mutated. The flanking sequence of each Tn5 mutant altered ISR activity is currently under investigation. These results indicate that gacS and mce are important genes in induction of ISR activity and drought resistance of P. chlororaphis O6. Our works will open opportunities for identification of bacterial genes or traits that are involved in ISR activity and induced drought resistance of P. chlororaphis O6.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2010.06a
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• pp.160-160
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• 2010

In this work, we investigate ohmic contacts to p-type GaN using a Pt/Cu/Au metallization scheme in order to achieve low resistance and thermally stable ohmic contact on p-GaN. An ohmic contact formed by a metal electrode deposited on a highly doped InGaN/GaN superlattice sturucture on p-GaN layer. The specific contact resistance is $1.56{\times}10^{-6}{\Omega}cm^2$ for the as-deposited sample, $1.35{\times}10^{-4}{\Omega}cm^2$ for the sample annealed at $250^{\circ}C$ and $6.88{\times}10^{-3}{\Omega}cm^2$ for the sample annealed at $300^{\circ}C$.

• Journal of the Korean Wood Science and Technology
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• v.18 no.4
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• pp.86-101
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• 1990

The primary objective of this research was to investigate the strength properties of Comply, a composite panel. fabricated with particle board as core material and veneer or plywood as face and back. 20types of comply composites were manufactured according to the four specific gravity levels(0.5, 0.6, 0.7 or 0.8) of particleboard core and three veneer or two plywood thicknesses for face and back. They were tested and compared with matching particleboard (control) on moisture content. specific gravity, bending properties(MOE, MOR SPL). nail resistance and internal bond strength. The obtained results were summarized as follows: The increasing effect of modulus of elasticity was shown by the increase of face and back veneer or plywood thickness. The modulus of rupture and stress at proportional limit of the comply composites bonded with 3mm thick veneers or 3mm thick plywood face and back were higher than 2mm thick veneer or 2mm thick plywood as face and back. Both of modulus of rupture and stress at proportional limit on bending of Comply were higher than those of control board. Also the modulus of elasticity of Comply showed much higher than that of control board. The nail resistance of Comply, composed of plywood as face and back was higher than that of veneer. The nail resistance of control board was higher than that of Comply at Sp.Gr 0.7 and 0.8 core boards. Internal bond of Comply, composed of 1mm and 2mm thick veneer as face and back was higher than that of 3mm thick veneer. The increasing effect of modulus of elasticity was shown by the increase of shelling ratio in Comply composed of veneer and plywood as face and back. The modulus of rupture was increased by the increment of shellmg ratio in Compiy, composed of plywood as face and back. The modulus of elasticity and modulus of. rupture of comply were higher than those of particleboard(control) in effect of shelling ratio. Therefore it was concluded that the mechanical property values of Comply were clearly greater than those of particleboard(control).

• BMB Reports
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• v.56 no.6
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• pp.347-352
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• 2023

The protein family of poly (ADP-ribose) polymerases (PARPs) is comprised of multifunctional nuclear enzymes. Several PARP inhibitors have been developed as new anticancer drugs to combat resistance to chemotherapy. Herein, we characterized PARP4 mRNA expression profiles in cisplatin-sensitive and cisplatin-resistant ovarian cancer cell lines. PARP4 mRNA expression was significantly upregulated in cisplatin-resistant ovarian cancer cell lines, and this upregulation was associated with the hypomethylation of specific cytosine-phosphate-guanine (CpG) sites (cg18582260 and cg17117459) on its promoter. Reduced PARP4 expression was restored by treating cisplatin-sensitive cell lines with a demethylation agent, implicating the epigenetic regulation of PARP4 expression by promoter methylation. Depletion of PARP4 expression in cisplatin-resistant cell lines reduced cisplatin chemoresistance and promoted cisplatin-induced DNA fragmentation. The differential mRNA expression and DNA methylation status at specific PARP4 promoter CpG sites (cg18582260 and cg17117459) according to cisplatin responses, was further validated in primary ovarian tumor tissues. The results showed significantly increased PARP4 mRNA expressions and decreased DNA methylation levels at specific PARP4 promoter CpG sites (cg18582260 and cg17117459) in cisplatin-resistant patients. Additionally, the DNA methylation status at cg18582260 CpG sites in ovarian tumor tissues showed fairly clear discrimination between cisplatin-resistant patients and cisplatin-sensitive patients, with high accuracy (area under the curve = 0.86, P = 0.003845). Our findings suggest that the DNA methylation status of PARP4 at the specific promoter site (cg18582260) may be a useful diagnostic biomarker for predicting the response to cisplatin in ovarian cancer patients.

• Journal of Korean Society of Environmental Engineers
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• v.28 no.3
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• pp.337-344
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• 2006

Characteristics of flocculent aggregates have great effects on membrane fouling. Floc from kaolin particles gave higher permeate throughputs than floc from natural particles at the same conditions. Therefore, the objectives of this study are to thoroughly analyze characteristics of flocculated aggregates and to investigate effects of flocculated aggregates on membrane fouling. Image analysis, specific rake resistance and cake compressibility were used for characterization of flocs. Different flocculent aggregates formed with natural and kaolin particles were employed in this study. The fractal dimensions from the image analysis were $D_2=1.79{\pm}0.07$ for floc from natural particles and $D_2=1.84{\pm}0.06$ for floc from kaolin particles. The lower fractal dimension($D_2$) of floc from natural particles indicated that the aggregates were more porous and less compact than floe from kaolin particles. In addition, both the specific cake resistances and compressible degrees of flocs from natural particles showed greater values than those of flocs from kaolin particles. The results implied that the porous and loose flocs from natural particles were more easily compressed on membrane surface than the dense and compact flocs from kaolin particles. The compressed flocs yielded the great hydraulic resistances by hindering the water flow through the cake layer.

• Korean Journal of Breeding Science
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• v.43 no.1
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• pp.42-49
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• 2011

QTL analysis for blast resistance was carried out using 140 $BC_3F_3$ lines derived from a cross between Ilpum as a recurrent parent and Moroberekan as a donor parent. 140 $BC_3F_3$ lines with the parents were inoculated with nine blast isolates. To identify QTLs for resistance to nine blast isolates, 134 SSR markers showing polymorphisms between the parents were genotyped for the 140 $BC_3F_3$ lines. A total of 17 resistance QTLs to nine isolates were detected on chromosomes 2, 3, 4, 6, 7, 9 and 10. The phenotypic variance explained by each QTL ranged from 8.2% to 26.4%. The Moroberekan alleles contributed the positive effect at these 17 QTL loci. In a previous study, the QTL, Pi45(t) for durable resistance to blast was identified using a sequential planting method. To know the relationship between Pi45(t) and the isolate-specific resistance gene, an $F_2$ population was developed from a cross between Ilpum and an introgression line harboring Pi45(t). $F_3$ lines segregating for the Pi45(t) were inoculated to three isolates. $F_3$ lines from the $F_2$ plants with the Moroberekan segment at the target region showed resistance to two isolates. This result seems to indicate that the Pi45(t) and the isolate-specific resistance gene are tightly linked or the resistance is controlled by the same gene(s). The markers linked to genes controlling blast resistance would be useful in developing blast resistance lines in the breeding program.

• Journal of Electrochemical Science and Technology
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• v.13 no.3
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• pp.369-376
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• 2022

In this study, the stability of an anion exchange membrane water electrolyzer (AEMWE) cell was evaluated in an on-off cycling operation with respect to an applied electric bias, i.e., a current density of 500 mA cm^-2, and an open circuit. The ohmic and polarization resistances of the system were monitored during operation (~800 h) using electrochemical impedance spectra. Specific consideration was given to the ohmic resistance of the cell, especially that of the membrane under on-off cycling conditions, by consistently feeding the cell with KOH solution. Owing to an excess feed solution, a momentary increase in the polarization resistance was observed immediately after the open-circuit. The excess feed solution was mostly recovered by subjecting the cell to the applied electric bias. Stability tests on the AEMWE cell under on-off cycling with continuous feeding even under an open circuit can guarantee long-term stability by avoiding an irreversible increase in ohmic and polarization resistances.

• The Korean Journal of Physiology and Pharmacology
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• v.19 no.5
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• pp.451-460
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• 2015

Sirtuin 1 (SIRT1) is a mammalian $NAD^+$-dependent protein deacetylase that regulates cellular metabolism and inflammatory response. The organ-specific deletion of SIRT1 induces local inflammation and insulin resistance in dietary and genetic obesity. Macrophage-mediated inflammation contributes to insulin resistance and metabolic syndrome, however, the macrophage-specific SIRT1 function in the context of obesity is largely unknown. C57/BL6 wild type (WT) or myeloid-specific SIRT1 knockout (KO) mice were fed a high-fat diet (HFD) or normal diet (ND) for 12 weeks. Metabolic parameters and markers of hepatic steatosis and inflammation in liver were compared in WT and KO mice. SIRT1 deletion enhanced HFD-induced changes on body and liver weight gain, and increased glucose and insulin resistance. In liver, SIRT1 deletion increased the acetylation, and enhanced HFD-induced nuclear translocation of nuclear factor kappa B (NF-${\kappa}B$), hepatic inflammation and macrophage infiltration. HFD-fed KO mice showed severe hepatic steatosis by activating lipogenic pathway through sterol regulatory element-binding protein 1 (SREBP-1), and hepatic fibrogenesis, as indicated by induction of connective tissue growth factor (CTGF), alpha-smooth muscle actin (${\alpha}$-SMA), and collagen secretion. Myeloid-specific deletion of SIRT1 stimulates obesity-induced inflammation and increases the risk of hepatic fibrosis. Targeted induction of macrophage SIRT1 may be a good therapy for alleviating inflammation-associated metabolic syndrome.