• Journal of Microbiology and Biotechnology
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• v.2 no.2
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• pp.141-151
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• 1992

Physiological changes of the murine hybridoma cell line $S3H5/\gamma{2bA2}$ during adaptation to RPMI 1640 medium with 1%(v/v) fetal bovine serum were characterized in terms of cell growth, antibody production, morphology, and metabolic quotients. Cells adapted to 1% serum medium in T-flasks became sensitive to shear induced by mechanical agitation and required at least 5% serum in the medium or spent medium for cell growth in spinner flasks, while cells adapted to 10% serum medium in T-flasks could grow in 1% serum medium in spinner flasks. Consequently, long-term adaptation to low serum media may not give the expected growth enhancement. After adaptation to 1% serum medium, changes in cell morphology were observed. The cells in 10% serum medium were uniform and circular, while cells in 1% medium were irregularly shaped. The DNA contents, which were measured by flow cytometry, were almost constant among the cells in the range of 1% to 10%. Further, no significant changes in energy metabolism and specific monoclonal antibody production rate were observed among these cells.

• Applied Chemistry for Engineering
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• v.25 no.4
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• pp.401-408
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• 2014

In membrane bio-reactor (MBR), the aeration control is one of the important independent variables to decrease fouling and to save energy with shear stress change on the membrane surface. The paper was carried out for numerical simulation of 3-dimensional fluid flow phenomena of the cylindrical bioreactor with submerged flat membranes equipped in the center and supplied the air from the bottom by using the COMSOL program. The viscosity and temperature of solution were assumed to be constant, and the specific air demand based on permeate volume ($SAD_p$) defined as scouring air per permeate rates was used as a variable. The calculated CFD velocities were compared with those of the velocity meter measurement and video image analysis, respectively. The results were good agreement each other within 11% error. For fluid flow in the reactor the liquid velocity increased rapidly between the air diffuser and membrane module, but the velocity decreased during flowing of the membrane module. Also, the velocity increased as it was near from the reactor wall to the central axis. The calculated shear stress on the membrane surface showed the highest value at the center part of the module bottom side and increased as aeration rate increased. Especially, the wall shear stress increased dramatically as the aeration rate increased from 0.15 to 0.25 L/min.

• Carbon letters
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• v.14 no.1
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• pp.58-61
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• 2013

In this work, the effect of catalysts on the mechanical properties of carbon fibers-reinforced epoxy matrix composites cured by cationic latent thermal catalysts, i.e., N-benzylpyrazinium hexafluoroantimonate (BPH) was studied. Differential scanning calorimetry was executed for thermal characterization of the epoxy matrix system. Mechanical interfacial properties of the composites were studied by interlaminar shear strength (ILSS), critical stress intensity factor ($K_{IC}$), and specific fracture energy ($G_{IC}$). As a result, the conversion of neat epoxy matrix cured by BPH was higher than that of one cured by diaminodiphenyl methane (DDM). The ILSS, $K_{IC}$, $G_{IC}$, and impact strength of the composites cured by BPH were also superior to those of the composites cured by DDM. This was probably the consequence of the effect of the substituted benzene group of BPH catalyst, resulting in an increase in the cross-link density and structural stability of the composites studied.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.33 no.12
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• pp.1014-1021
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• 2009

A new eddy viscosity equation was formulated from assumption of turbulence length scale equation and specific dissipation ratio equation. Then, a set of turbulence model equations for the turbulent kinetic energy ${\kappa}$, the viscosity ${\nu}_t$, and the intermittency factor ${\gamma}$ is proposed by considering the entrainment effect. Closure coefficients are determined by experimental data and resorting to numerical optimization. Present model has been applied to compute four representative cases of free shear flows and successfully compared with experimental data. In particular, the spreading rate, the centreline mean velocity and the profiles of intermittency are calculated with improved accuracy. Also, the proposed ${\nu}_t-{\kappa}-{\gamma}$ model was applied to channel flow by considering the wall effect and the results show good agreements with the Direct Numerical Simulation data.

• Proceedings of the Korean Society of Machine Tool Engineers Conference
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• 2003.10a
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• pp.77-82
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• 2003

In end milling process, undeformed chip thickness and cutting force vary periodically with phase change of the tool. Recently, a model has been proposed to simulate the shear and friction characteristics of an up-end milling process in terms of the equivalent oblique cutting to this. In the current study, the varying undeformed chip thickness and the cutting forces in a down-end milling process have been replaced with the equivalent ones of oblique cutting. And, the down-end milling characteristics of SM45C has been compared with that of the up-end milling previously presented with different helix angles.

• Journal of the Korean Society of Propulsion Engineers
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• v.15 no.5
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• pp.96-106
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• 2011

There are many advantages in applying gel propellant to a gel propulsion system. These include higher performances, the energy management of liquid propulsion system, reliable storability and low leakage characteristics. Additionally, gel propulsion system are preferable to the high density impulse of propulsion system. Also, when compared to liquid propellants, the gel propellants acquire greater heat energy. Gel propellants achieve a high specific impulse when metal particles with aluminum and boron are added. With respect to atomization, an inactive process occurs due to the variable viscosity of the metal particles and gelling agents. To improve the defect of atomization and combustion characteristics of gel propellant, a variety of issues related to spray and combustion is introduced here.

• Polymer(Korea)
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• v.24 no.4
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• pp.499-504
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• 2000

The effect of anodic oxidation on high strength PAN-based carbon fibers has been studied in terms of surface functionality and surface energetics of the fiber surfaces, resulting in improving the mechanical properties of composites. According to FT-IR and XPS measurements, it reveals that the oxygen functional groups on fiber surfaces induced by an anodic oxidation largely influence the surface energetics of fibers or the mechanical interfacial properties of composites, such as the interlaminar shear strength (ILSS) of composites. According to the contact angle measurements based on the wicking rate of a test liquid, it is observed that anodic oxidation does lead to an increase in surface free energy of the carbon fibers, mainly due to the increase of its specific (or polar) component. From the surface energetic point of view, it is found that good wetting plays an important role in improving the degree of adhesion at interfaces between fiber and epoxy resin matrix of the resulting composites. Also, a direct linear relationship is shown between 01s/01s ratio and ILSS or between specific component and ILSS of the composites for this system.

• Structural Engineering and Mechanics
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• v.44 no.5
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• pp.611-631
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• 2012

The passive energy dissipation technology has been proven to be reliable and robust for recent practical applications. Various dampers or energy dissipation devices have been widely used in building structures for enhancing their performances during earthquakes, windstorm and other severe loading scenarios. This paper presents a simplified seismic design procedure for retrofitting earthquake-damaged frames with viscous dampers. With the scheme of designing the main frame and the supplemental viscous dampers respectively, the seismic analysis model of damped structure with viscous dampers and braces was studied. The specific analysis process was described and approach to parameter design of energy dissipation components was also proposed. The expected damping forces for damped frame were first obtained based on storey shear forces; and then they were optimized to meet different storey drift requirements. A retrofit project of a RC frame school building damaged in the 2008 Wenchuan earthquake was introduced as a case study. This building was retrofitted by using viscous dampers designed through the simplified design procedure proposed in this paper. Based on the case study, it is concluded that this simplified design procedure can be effectively used to make seismic retrofit design of earthquake-damaged RC frames with viscous dampers, so as to achieve structural performance objectives under different earthquake risk levels.

• KSBB Journal
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• v.8 no.5
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• pp.431-437
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• 1993

Active carbon which has the smallest bulk and wet density was found as the best support media among 4 different kinds of materials(celite, natural zeolite, Pusuk stone, active carbon) to make a proper fluidized-bed with small energy consumption. Its minimum and optimum fluidization velocity were found as 0.03cm/sec and 0.25cm/sec, respectively. As organic loading rate for methane fermentation was increased, CODcr removal efficiencies of all the media were decreased. But, CODcr, removal efficiencies of active carbon was maintained more than 90% in this experimental range of the organic loading rate. Larger amount of microorganism was adsorbed on the active carbon which has very high specific surface area. At the organic loading rate of 16g CODcr,/l day, its adsorbed cell mass was 157mg/g. Comparing natural zeolite with roast celite, adsorbed cell mass did not increase in proportion to specific surface area of the media. Even though roast celite has the same specific surface area as the Pusuk stone, its organic removal ability was superior to that of the Pusuk stone, which explains that the relatively great surface roughness and the positive surface charge are important for cell adsorption. It was concluded that the support media for anaerobic fluidized reactor should have small wet density and small fuidization velocity, if possible, in order to increase cell adsorption by reducing the fluid shear stress.

• Journal of the Korean GEO-environmental Society
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• v.14 no.5
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• pp.33-39
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• 2013

A drilling exploration in deep sea is being processed to develop new energy resource in the world. In 2007, the presence of the gas hydrate had been confirmed during the UBGH1 (Ulleung Basin Gas Hydrate Expedition 1) in the Ulleung Basin. Geotechnical properties of the deep marine sediment are important factors for assessing the safety of gas production facility and productivity from the hydrate bearing sediment. In this study, comprehensive laboratory tests are conducted to investigate the geotechnical engineering characteristics of the deep marine sediments recovered from the hydrate occurrence regions during the UBGH2 (Ulleung Basin Gas Hydrate Expedition 2) in the Ulleung Basin, East Sea, Korea. The index properties of the specimens including the specific gravity, atterberg limits, specific surface, and particle size distribution are measured, and these are compared to the results reported by previous studies. A zero-lateral strain cell, which houses bender elements, is used to determine stress-dependant characteristics and shear wave velocities with the vertical effective stresses. Furthermore, the hydraulic conductivity is calculated based on the consolidation test results.