• Journal of Life Science
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• v.14 no.3
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• pp.429-434
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• 2004

Levansucrase gene (levU) from Zymomonas mobilis was subcloned downstream of GALl promoter in pYES 2.0 and pYInu-AT [GALl0 promoter＋exoinulinase signal sequence of Kluyveromyces marxianus], resulting pYES-levU and pYInu-levU, respectively. The two expression plasmids were introduced into an invertase-deficient strain, Saccharomyces cerevisiae SEY2102, and then transformants showing high activity of levansucrase were selected. When each yeast transformants was cultivated in medium containing galactose, the extracellular and intracellular activities of levansucrase reached about 7.17 U/㎖ with the strain harboring pYES-levU and 6.61 U/㎖ with the strain harboring pYInu-levU. It was found that about 50% of levansucrase were detected in the medium and periplasmic space, and exoinulinase signal sequence didn't enhance the secretion efficiency. Furthermore, the recombinant levansucrase expressed in yeast seems to be produced as a hyper-glycosylated form.

• KSBB Journal
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• v.11 no.2
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• pp.165-172
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• 1996

To obtain a correctly folded human proinsulin, export of proinsulin using Staphylococcal protein A signal sequence-mediated secretion pathway has been attempted in E.coli. A secretion operon for proinsulin was constructed by consecutively connecting T7 promoter, SPA ribosome binding site, SPA signal sequence gene, and human proinsulin gene. Little immunoreactive proinsulin was detected in the periplasmic space and. culture medium, and not even in cytoplasmic space. The qualitative analysis of transcribed proinsulin mRNA and the in vitro transcription/translation experiment suggests that the negligible level of proinsulin export appears to be due to intracellular degradation of proinsulin, rather than due to the blockage during translocation. However, expression of proinsulin fusion protein such as MBP-proinsulin could dramatically increase export of proinsulin in E.coli.

• Economic and Environmental Geology
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• v.51 no.5
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• pp.455-461
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• 2018

The purpose of this study is to compare the expert group's recognition with general people's recognition about the image of the future on geo-technoloies. The survey targeting to 215 experts and to 598 people had been completed from July to August, 2017 by a research firm. The research result showed that energy was selected as the first priority by expert group and climate environment, geologic environment, mineral resources and material, living place, space-earth research, and the fourth industrial revolution were sequence ranked by experts. Also, it was analyzed that climate environment was recognized as the first priority and geologic environment, energy, mineral resources and material, living place, space-earth research, the fourth industrial revolution were ranked in sequence by general people group.

• Microbiology and Biotechnology Letters
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• v.32 no.3
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• pp.206-211
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• 2004

Levansucrase gene(lscA) from Pseudomonas aurantiaca was subcloned downstream of GAL1 promoter in pYES 2.0 and pYInu-AT [GAL10 promoter ＋ exoinulinase signal sequence of Kluyveromyces marxianus], resulting pYES-lscA and p YInu-lscA, respectively. The two expression plasmids were introduced into an invertase-deficient strain, Sacchromayces cerevisiae SEY2102, and transformants with high activity of levansucrase were selected. When each yeast transform ants was cultivated in medium containing galactose, the extracellular and intracellular activities of levansucrase reached about 8.62 U/ml with the strain harboring pYES-lscA and 5.43 U/ml with the strain harboring pYInu-lscA. The levansucrase activity of 80% was detected in the periplasmic space and cytoplasm. The levansucrase activity in the medium of SEY2102/pYInu-lscA was 0.87 U/ml whereas that of SEY2102/pYES-lscA was 0.47 U/ml, which implying the exoinulinase signal sequence didn't enhance the secretion efficiency of levansucrase. Furthermore, the recombinant levansucrase expressed in yeast seems to be produced as a hyper-glycosylated form.

• Journal of Astronomy and Space Sciences
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• v.19 no.1
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• pp.47-56
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• 2002

Observations of times of minimum lights of the eclipsing binary V65l Cas were carried out on the three nights from November 21-23, 2000. From our observations a total of seven new times of minimum lights was obtained. Through the analysis of photoelectric and CCD times of minima of V65l Cas including ours, the light time orbit due to a third body, which was propose by Kim ＆ Lee (2000), was confirmed and improved. The resultant values for the period, semi-amplitude, and eccentricity of the light-time orbit were $6.^{y}3,\;0.^{d}0013$, and 0.78, respectively. The deduced marts range of the third body is . If the third body is $0.09M_{\odot}\;{\leq}\;M_3\;{\leq}\;0.20M_{\odot}\;for\;i_3\;{\geq}\;30^{\circ}$. If the third body suggested in V65l Cas system exists really and is a main-sequence star, it is located at the end of the main-sequence.

• Journal of Astronomy and Space Sciences
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• v.18 no.3
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• pp.175-190
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• 2001

We investigated population of the elliptical galaxy NGC 4649 using the spectral synthesis technique based on the linear program in the spectral regions between $3160\AA$to $10800\AA$. We used the spectral data of stars obtained by Gunn & Striker (1983), and the integrated spectrum of NGC 4649 observed by Bertola et al. (1982). Among four models with different main sequence turn-off points, G8-K0V main sequence turn-off model is best fitted to the integrated spectrum of NGC 4649. We also found that super metal rich K giants are needed to describe the absorption lines in the long wavelength regions of integrated spectrum of NGC 4649. The mass to absolute light ratio obtained from the spectral synthesis is ~ 20 similar to those calculated dynamically.

• Communications of the Korean Mathematical Society
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• v.25 no.1
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• pp.119-128
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• 2010

Let ${X_n,\;n\geq1}$ be a sequence of independent identically distributed (i.i.d.) random variables (r.vs.), defined on a probability space ($\Omega$,A,P), and let ${N_n,\;n\geq1}$ be a sequence of positive integer-valued r.vs., defined on the same probability space ($\Omega$,A,P). Furthermore, we assume that the r.vs. $N_n$, $n\geq1$ are independent of all r.vs. $X_n$, $n\geq1$. In present paper we are interested in asymptotic behaviors of the random sum $S_{N_n}=X_1+X_2+\cdots+X_{N_n}$, $S_0=0$, where the r.vs. $N_n$, $n\geq1$ obey some defined probability laws. Since the appearance of the Robbins's results in 1948 ([8]), the random sums $S_{N_n}$ have been investigated in the theory probability and stochastic processes for quite some time (see [1], [4], [2], [3], [5]). Recently, the random sum approach is used in some applied problems of stochastic processes, stochastic modeling, random walk, queue theory, theory of network or theory of estimation (see [10], [12]). The main aim of this paper is to establish some results related to the asymptotic behaviors of the random sum $S_{N_n}$, in cases when the $N_n$, $n\geq1$ are assumed to follow concrete probability laws as Poisson, Bernoulli, binomial or geometry.

• Journal of Navigation and Port Research
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• v.30 no.6 s.112
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• pp.457-464
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• 2006

Remarshalling operation is one of the operations considered important in an automated container terminal to perform quickly loading operations and delivery operations. It arranges the containers scattered at a verticla yard block in order to reduce the transfer time and the rehandling time of ATC(Automated Transfer Crane)s. This paper deals with the remarshalling planning problem minimizing the weighted operation time. This problem can be decomposed into 2 subproblems, storage space assignment problem and operation sequencing problem Storage space assignment problem decides to where containers are transported in terms of transportation time cost.. With results of a previous subproblem, operation sequence problem determines the ATC operation sequence, which minimizes the dead-heading of ATC This study formulates each subproblem with mixed integer program and dynamic program. To illustrate the proposed model, we propose an instance to explain the process of remarshalling planning.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.13 no.5
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• pp.922-930
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• 2009

An optimized PWM switching strategy for an induction motor voltage control is developed and demonstrated. Space vector modulation in voltage source inverter offers improved DC-bus utilization and reduced commutation losses and has been therefor recognized the preferred PWM method especially in case of digital implementation. An optimized PWM switching strategy for an induction motor voltage control consists of switching between the two active and one zero voltage vector by using the proposed optimal PWM algorithm. The preferred switching sequence is defined as a function of the modulation index and period of a carrier wave. The sequence is selected by using the inverter switching losses and the current ripple as the criteria. For low and medium power application, the experimental results indicate that good dynamic response and reduced harmonic distortion can be achieved by increasing switching frequency.

• Journal of Korean Tunnelling and Underground Space Association
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• v.13 no.3
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• pp.215-231
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• 2011

In this study, the behavior of a shallow 2-arch tunnel during the excavation in the sandy ground containing vertical discontinuity plane was experimentally studied. Load transfer mechanism in the pillar caused by a 2-arch tunnel excavation was observed. The position of the vertical discontinuity plane was varied. Model tests were carried out in the normal construction sequence of 2-arch tunnel. Test results-showed that the load transfer caused by the 2-arch tunnel excavation was concentrated in the discontinuity plane, and was cut by the discontinuity plane, so no load transfer took place above the discontinuity plane. It was also shown that the effect of adjacent tunnel excavation on the pillar load and the ground deformation was greater when excavating the upper half-face of the main tunnel, more than when excavating the lower half-face.