• Animal cells and systems
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• v.9 no.4
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• pp.205-209
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• 2005

A rapid, simple and efficient method to extract RNA from the adult polyps of a soft coral, marine cnidarian, Scleronephthya gracillimum $(K\ddot{u}kenthal)$; was developed in this study. The highest yield and purity of RNA was obtained with the lysis solution containing 35 mM EDTA, 0.7 M LiCl, 7.0% SDS, and 200 mM Tris-Cl (pH 9.0). Approximately $40{\mu}g$ of total RNA was extracted from 200 mg of liquid nitrogen-pulverized polyp tissue. The ratio of absorbance at 260 nm and 280 nm ranged from 1.8 to 2.0. The results of the reverse transcription polymerase chain reaction (RTPCR) with ${\beta}-actin$ gene specific primers and Northern blot analysis using the same gene probe revealed that the RNA extracted by our method had high quality, and was sufficient for subsequent molecular biological analyses. This method was effective for RNA extraction from other soft coral species which belong to the genus Dendronephthya.

• Ocean and Polar Research
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• v.45 no.3
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• pp.141-153
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• 2023

Biological pump processes generated by diatom production in the surface water of the Southern Ocean play an important role in exchanging CO₂ gas between the atmosphere and ocean. In this study, the biogenic opal content of the sediments was measured to elucidate the variation in the primary production of diatoms in the surface water of the Southern Ocean since the last glacial period. A piston core (COR-1bPC) was collected from the Conrad Rise, which is located in the Indian sector of the Southern Ocean. The sediments were mainly composed of siliceous ooze, and sediment lightness increased and magnetic susceptibility decreased in an upward direction. The biogenic opal content was low (38.9%) during the last glacial period and high (73.4%) during the Holocene, showing a similar variation to that of Antarctic ice core ΔT and CO₂ concentration. In addition, the variation of biogenic opal content in core COR-1bPC is consistent with previous results reported in the Antarctic Zone, south of the Antarctic Polar Front, in the Southern Ocean. The glacial-interglacial biogenic opal production was influenced by the extent of sea ice coverage and degree of water column stability. During the last glacial period, the diatom production was reduced due to the penetration of light being limited in the euphotic zone by the extended sea ice coverage caused by the lowered seawater temperature. In addition, the formation of a strong thermocline in more extensive areas of sea ice coverage led to stronger water column stability, resulting in reduced diatom production due to the reduction in the supply of nutrient-rich subsurface water caused by a decrease in upwelling intensity. Under such environmental circumstances, diatom productivity decreased in the Antarctic Zone during the last glacial period, but the biogenic opal content increased rapidly under warming conditions with the onset of deglaciation.

• Journal of Korean Society for Geospatial Information Science
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• v.23 no.1
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• pp.65-71
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• 2015

Red Tide phenomenon which happens in the southern coast of Korea gives massive damage to the fishermen who run fish farms and thereby a lot of efforts to prevent damage are made from various angles. In particular, red tide monitoring with satellite imagery can make it possible to obtain the occurrence data of red tide throughout the whole areas of the sea, which helps provide important information for establishing the preventive plans of disasters. In this regard, this study selected the South Sea of Gyeongnam Province with a view to suggesting the monitoring results with regard to the spread and reduction of the Red Tide in the middle of the day by using the GOCI Images of COMS. With this intention, it selected the region in the South Sea of Gyeongnam Province. The study results of analysis on the GOCI image data for the years of 2013(Aug. 12) and 2014 (Sep. 11) are as follows: the pattern of the Red Tide in the region of the South Sea occurred in the southern sea area of Geoje-do in the morning. It gradually spread and showed a gradual decline after reaching the top at 1 PM. In addition, in terms of the tide movement in the middle of the day, Red Tide began in the southern sea area and moved to the west, and moved to the east again at noon. It is judged that additional study on many factors such as the characteristics of the future Red-tide organisms, tidal currents, amount of sunshine, and water temperature is needed, but it is estimated that Red Tide movement monitoring with GOCI images would provide very crucial information for predicting the spread and movement of the Red Tide to protect and manage the Red Tide disasters.

• Korean Journal of Animal Reproduction
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• v.25 no.4
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• pp.359-369
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• 2001

Polymerase chain reaction (PCR) amplification of DNA as 30 different arbitrary primers and random amplified polymorphic DNAs (RAPD) analysis were performed on genomic DNA extracted from the blood of the marine rockfish (Sebastes schlegeli) from the Yellow Sea and the Southern Sea. The unique properties of the genomic DNA were used to investigate the features of the population dynamics and origins of the species. Out of 30 primers, seven generated 207 highly reproducible RAPD polymorphic products, producing approximately 2.7 polymorphic bands per primer. About 67.4% of total amplified products (307) were either polymorphic (207) to rockfish. The degree of similarity varied from 0.22 to 0.63 as calculated by bandsharing analysis. Also, the average level of bandsharing was 0.39$\pm$0.02 within the rockfish strains. The electrophoretic analysis of RAPD-PCR products showed the relatively high levels if variation between different individuals in rockfish from the Yellow Sea. However, the RAPD outlines obtained with DNA of different rockfish strains from the Yellow Sea and the Southern Sea in Korea were very similar. Also, a small number of polymorphic bands were identified. Even if further analyses or more rockfish populations are required, this result implies RAPD analysis reflects genetic differences between the geographical strains of the rockfish.

• Journal of the Korean Society of Marine Environment & Safety
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• v.9 no.2
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• pp.65-72
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• 2003

Oceanic conditions of the Tsushima Warm Current (TWC) region in the southern area of the East Sea (Japan Sea) are examined using data obtained from a CREAMS (Circulation Research if the East Asian Marginal Seas) cruise in June 1996. In 1990s, a lower temperature appears in 1996 and in this period, two branches of the TWC exist and the first branch of the TWC flows inshore of the Japanese coastal region compared to that in the other years, especially in the shallower water layer at depth less than about 200 m. The TWC cored with the higher salinity (>34.6 psu) is clearly observed over the continental shelf in the Japanese coastal region and offshore and identified by geostrophic calculation. Intrusion of the TWC into the East Sea through the Korea Strait (the Tsushima Strait) makes the density structure in the water column change and the water mass in the TWC region is unstable based on Brunt­Vaisala frequency.

• Journal of Environmental Science International
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• v.20 no.9
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• pp.1141-1149
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• 2011

The three-dimensional hydrodynamic model was simulated to understand coastal sea front of formation and seasonal variation in the Southern Sea of Korea. In this study, we used to concept of stratification factor, to realize seasonal distribution of stratification coefficient which of seasonal residual flow, considered with, tide, wind and density effect. Tidal current tends to flow westward during the flood and eastward during ebb. The current by the wind stress showed to be much stronger the coastal than the offshore area in the surface layer. And the current by the horizontal gradient of water density showed to be relatively weak in the coastal area, with little seasonal differences. On the other hand, the flow in the offshore area showed results similar to that of the Tsushima Warm Current. The stratification factor (SHv) was calculated by taking into account the total flow of tide, wind and density effect. In summer, the calculated SHv distribution ranged from 2.0 to 2.5, similar to that of the coastal sea front. The horizontal temperature gradient showed to be strong during the winter, when the vertical stratification is weak. On the other hand, the horizontal gradient became weak in summer, during which vertical stratification is strong. Therefore, it is presume that the strength of vertical stratification and the horizontal temperature gradient affect the position of the coastal sea front.

• Korean Journal of Ichthyology
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• v.27 no.3
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• pp.238-242
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• 2015

Three specimens (56.7~92.4 mm in standard length) of the small psychrolutid Psychrolutes pustulosus (Schmidt, 1937) were collected by bottom trawl in the coastal waters off Pohang, the southern East Sea, Korea. This species is characterized by large cephalic pores, no markings on the body or fins, no spines on the head, and no notch between the spiny and soft-rayed dorsal fin. We describe specimens the southernmost distribution in the world, and compare them with two congeneric species from Russia. We thus present the first record of Psychrolutes pustulosus (Schmidt, 1937) within the Korean fish fauna and propose the new Korean name "Min-mu-nui-mul-su-bae-gi" for this species.

• Ocean and Polar Research
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• v.34 no.1
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• pp.37-51
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• 2012

We investigated the distribution and species composition of zooplankton in relation to hydrographical characteristics in the Korea Strait during the winter (February) and summer (July) of 2009. Satellite images of sea surface temperatures and in situ CTD data showed that the southeastern water zone (St3-5) off Jeju Island was strongly influenced by the Tsushima Current during both the winter and summer, whereas the Changjiang Diluted Water, characterized as water with relatively low salinity, was evident in the coastal waters of Jeju Island during the summer. During winter, zooplankton abundance was significantly higher than in the summer, with dominance by copepods, ostracods, siphonophorans, appendicularians, and nauplii. In both seasons, copepods represented >70% of the total zooplankton population. Calanus sinicus, a large calanoid copepod, was dominant in near the coast, and that may be associated with the intrusion of low salinity water (i.e., the Changjiang Diluted Water) along the coast. The abundance of P. parvus s.l. and A. omorii, known as neritic copepods, was mainly associated with the Korea Southern Coastal Water. Foraminiferans, Ostracods, O. plumifera, and P. aculeatus were concentrated in the southeastern water off Jeju Island during both seasons; showing their association with the Tsushima Current, which is characterized warm, high salinity water. Our results suggest that the distribution, abundance, and species composition of zooplankton are highly influenced by different water masses in the Korea Strait.

• 한국해양학회지
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• v.24 no.1
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• pp.1-14
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• 1989

This study analyzes the interannual periodicity by using the statistical techniques of probability, spectral analysis, empirical orthogonal function analysis (EOF), and coherency analysis. The data base for this study is the time series of 1971-1985 temperature, salinity in the southern waters of the East Sea, 1960-1986 mean sea level at Pusan and Izuhara, and 1960-1986 sea level atmospheric pressure at Pusan. The appearances of anomalous temperatures higher and lower than 15-year mean monthly average with one standard deviation are about 30% of total data. The significant interannual period for temperature, salinity and sea level fluctuation is 36.6, and 23.3 months. The empirical orthogonal function analyses show that the 1st mode of the EOFs is responsible for more than 90% of total variance of the surface temperature variations, while in near-bottom waters, the relative importance of the higher EOF modes is much greater explaining more than 30% of total variance. The coherency between normalized temperatures and salinities is significant at the interannual period of 36.6 and 21.3 months.

• Parasites, Hosts and Diseases
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• v.53 no.3
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• pp.349-353
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• 2015

Anisakis simplex sensu stricto (s.s.), Anisakis pegreffii, Anisakis berlandi (=A. simplex sp. C), and Anisakis typica are the 4 major species of Anisakis type I larvae. In the Republic of Korea (Korea), A. pegreffii, A. berlandi, and A. typica larvae in fish hosts has seldom been documented. In this study, molecular analysis was performed on Anisakis larvae from the sea eels (Astroconger myriaster), the major source of human anisakiasis in Korea, collected from Tongyeong City, a southern coastal area of Korea. All 20 sea eels examined were infected with Anisakis type I larvae (160 larvae; 8 per fish). Their species were analyzed using PCR-RFLP patterns and nucleotide sequences of internal transcribed spacers (ITS1, 5.8 subunit gene, and ITS2) and mitochondrial cytochrome c oxidase 2 (cox2). Most (86.8%; 112/129) of the Anisakis type I larvae were A. pegreffii, and 7.8% (10/129) were A. typica. The remaining 5.4% (7/129) was not identified. Thus, A. pegreffii is the major species of anisakid larvae in sea eels of the southern coast of Korea.