• Nutrition Research and Practice
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• 제15권2호
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• pp.248-265
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• 2021

BACKGROUND/OBJECTIVES: This study aimed to assess the dietary behaviors of preschool children using the nutrition quotient for preschoolers (NQ-P) and analyzed the difference in the scores of the children's dietary behaviors in regards to the parents' health consciousness. SUBJECTS/METHODS: The subjects were 257 children aged 3-5 years and their parents residing in Seoul and Gyeonggi-do, South Korea. The questionnaire is composed of demographic characteristics, the NQ-P questions, and health consciousness. All data were statistically analyzed by SPSS program (ver. 25.0) and the statistical differences in variables were evaluated by χ2 test, Fisher's exact test, t-test, 1-way analysis of variance and Tukey's multiple comparison test. RESULTS: There was a significant difference on the intake frequency of processed meat by region and fast foods by age, region, and weight status (P < 0.05). There was also a significant difference in not moving around while eating by weight status and parents' effort to have healthy eating habits by sex (P < 0.05). The mean score of NQ-P of the total subjects was 59.47, which was within the medium-low grade. The mean score of balance was 61.62, and the boys were significantly higher than girls (P < 0.05). As the age of children increased, the mean score of the moderation was significantly decreased (P < 0.05). The scores of NQ-P (P < 0.05), balance (P < 0.01), and environment (P < 0.05) were significantly higher in the high group of parents' health consciousness than the low group. CONCLUSIONS: According to the results of the evaluation by NQ-P, the dietary behaviors of preschoolers residing in Seoul and Gyeonggi-do need to be improved. For improving their eating behavior and nutritional health status, parents and children need customized nutrition education programs based on sex, age, region and weight status of preschool children as well as the degree of parents' health consciousness.

• 인간식물환경학회지
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• 제24권5호
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• pp.485-498
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• 2021

Background and objective: This study was conducted to find out the status of plant utilization in the current textbooks by analyzing the plants by grade and subject in the national textbooks for all elementary school grades in the 2015 revised curriculum in Korea. Methods: The data collected was analyzed using Microsoft Office Excel to obtain the frequency and ratio of collected plant data and SPSS for Windows 26.0 to determine learning content areas by grade and the R program was used to visualize the learning content areas. Results: A total of 232 species of plants were presented 1,047 times in the national textbooks. Based on an analysis of the plants presented by grade, the species that continued to increase in the lower grades tended to decrease in the fifth and sixth grades, the upper grades of elementary school. As for the number and frequency of plant species by subject, Korean Language had the highest number and frequency of plant species. The types of presentation of plants in textbooks were mainly text, followed by illustrations and photos of plants, which were largely used in first grade textbooks. In addition, as for the area of learning contents in which plants are used, in the lower grades, plants were used in the linguistic domain, and in the upper grades, in the botanical and environmental domains of the natural sciences. Herbaceous plants were presented more than woody plants, and according to an analysis of the plants based on the classification of crops, horticultural crops were presented the most, followed by food crops. Out of horticultural crops, flowering plants were found the most diversity with 63 species, but the plants that appeared most frequently were fruit trees that are commonly encountered in real life. Conclusion: As a result of this study, various plant species were included in elementary school textbooks, but most of them were horticultural crops encountered in real life depending on their use. Nevertheless, plant species with high frequency have continued a similar trend of frequency from the previous curriculums. Therefore, in the next curriculum, plant learning materials should be reflected according to social changes and students' preference for plants.

• Mass Spectrometry Letters
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• 제13권4호
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• pp.133-138
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• 2022

Taurine is a type of sulfur-containing amino acid having a sulfate functional group, that is biosynthesized from cysteine. It is mainly distributed in high concentrations in animal tissues and is known to have various effects such as osmotic pressure control, calcium control, anti-inflammatory, antioxidant, and hepatocellular protection. Also, taurine deficiency causes a variety of symptoms, including visual impairment. In particular, in the case of cats, taurine is not biosynthesized and must be supplied through food, so it is classified as an essential amino acid. In this study, an analysis method using mass spectrometry was developed instead of the commonly used derivatization method to quickly, environmentally, and precisely analyze taurine in various animal feeds. The developed analytical method showed good linearity (R² > 0.99), accuracy (81.97-105.78%), and precision (0.07-12.37%). In addition, the developed method was further verified through quantitative comparison with the derivatization method. This developed method was used in the determination of taurine in 20 animal feed samples obtained from South Korea. The levels of taurine found ranged from 81.53 to 6,743.53 mg/kg. The developed analysis method will be used for the detection and quantification of taurine in domestic feed.

• Fisheries and Aquatic Sciences
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• 제26권2호
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• pp.158-168
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• 2023

The global output of Pyropia yezoensis (dried seaweed or laver, also called 'Gim' in Korea) has been reduced over the half-decade due to the wide spread of red rot disease, a serious algal disease affecting P. yezoensis. Recently, Gold 1 (G1), which is a resistant strain of P. yezoensis to red rot disease, was developed and commercialized in South Korea, yet its physiological activity has not been investigated. In this study, a comparative study was performed on G1 and commercially available strain of P. yezoensis (CP) for their antioxidative activities. Aqueous extract of G1 showed more marked 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity compared to that of CP. In 293T cells, antioxidant activity against H₂O₂-induced reactive oxygen species (ROS) formation was only observed in G1 extract. In addition, G1 extract showed more potent inhibitory effect on H₂O₂-induced apoptotic cell death than CP extract, as examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and fluorescence microscopy. Expression levels of various apoptosis-related genes, including B-cell lymphoma 2-associated X protein, p53, capase-3, and inflammatory cytokines, in H₂O₂-treated cells were significantly decreased by the treatment of G1. Taken together, the present study suggests that a new strain of red seaweed G1 can recover oxidative stress effectively by improving the imbalance of ROS generation and has a potential to be used a functional ingredient as an antioxidant source.

• 한국프랜차이즈경영연구
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• 제1권1호
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• pp.1-17
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• 2010

프랜차이즈 가맹점의 계약 전 기대와 운영 후 지각된 성과에 대한 부정적 차이인식(gap)의 정도가 커지면 분쟁의 원인으로 발전할 수 있는데, 이는 근본적으로 가맹본부가 신규 가맹점 모집에 급급한 나머지 계약전 기대를 너무 높게 만들어 운영 후 지각된 성과와의 부정적 차이인식(gap)이 커지면서 발생하는 경우가 높다고 볼 수 있다. 따라서 본 연구에서는 가맹본부의 관계지향성에 대한 가맹점의 계약 전 기대와 운영 후 지각된 성과의 차이인식(gap)을 통해 가맹본부의 관계지향성에 대한 가맹점의 만족도를 간접적으로 측정하고, 관계지향성에 대한 차이인식(gap)이 프랜차이즈 업종별로 어떻게 다르게 나타나는지 알아보고자 한다. 본 연구의 실증분석 결과, 관계지향성에 대한 차이인식(gap) 평균이 모두 음(-)으로 나타난 것은 운영 후 지각된 성가가 계약 전 기대보다 낮다는 것을 의미하는 것으로, 가맹점 계약 전 기대관리의 필요성과 함께 프랜차이즈 업종별로 관계지향에 대한 차이인식(gap)이 다르게 나타났다는 것을 밝혔다는 데에서 그 의의를 찾을 수 있다. 이런 점들로 볼 때, 프랜차이즈는 가맹본부와 가맹점 간의 계약관계에 의해서 유지되는 시스템으로 지속적인 관계를 유지하기 위해서는 많은 노력과 함께 기대관리가 필요하다.

• 분석과학
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• 제21권5호
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• pp.424-431
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• 2008

스트렙토마이신은 아미노글리코사이드계 항생제중의 하나로써 가축, 가금류의 질병 치료와 예방에 널리 사용되고 있다. 국내에서는 양봉용으로는 허가되어 있지 않지만 일부 국가에서는 유럽형 부저병과 같은 세균성 벌꿀 질병의 치료에 사용되는 것으로 알려져 있다. LC-MS/MS를 이용하여 벌꿀 중에 잔류하는 스트렙토마이신(streptomycin)을 분석하는 방법을 확립하였다. 확립된 분석법은 재현성과 정밀성을 높이고 미량까지 검출할 수 있도록 회수율을 높이기 위하여 정제 및 추출과정을 최적화 하였으며, 효과적인 분석을 위해서 액체 크로마토그래피(HPLC) 와 텐뎀 질량분석법(tandem mass spectrometry)의 조건들을 최적화하였다. 확립된 방법은 5.0~50.0 ug/kg 농도에서 5.5~14%인 정밀성(RSD)을 나타내었으며, bias로써 -10.0~8.0%의 정확도를 나타내었다. 한편, 스트렙토마이신을 벌꿀 공 시료에 10.0 ug/kg의 농도로 소량첨가한 후 얻은 회수율은 74%이었고, 정량한계(LOQ)는 0.75 ug/kg이었다. 확립된 벌꿀 중 스트렙토마이신 분석법을 이용하여 실제 벌꿀시료에 적용한 결과 몇몇 시료에서 소량의 스트렙토마이신이 검출되었다.

• Mycobiology
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• 제51권4호
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• pp.195-209
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• 2023

The seed borne disease such as bakanae is difficult to control. Crop yield loss caused by bakanae depending on the regions and varieties grown, ranging from 3.0% to 95.4%. Bakanae is an important disease of rice worldwide and the pathogen was identified as Fusarium fujikuroi Nirenberg (teleomorph: Gibberella fujikuroi Sawada). Currently, four Fusaria (F. fujikuroi, F. proliferatum, F. verticillioides and F. andiyazi) belonging to F. fujikuroi species complex are generally known as the pathogens of bakanae. The infection occurs through both seed and soil-borne transmission. When infection occurs during the heading stage, rice seeds become contaminated. Molecular detection of pathogens of bakanae is important because identification based on morphological and biological characters could lead to incorrect species designation and time-consuming. Seed disinfection has been studied for a long time in Korea for the management of the bakanae disease of rice. As seed disinfectants have been studied to control bakanae, resistance studies to chemicals have been also conducted. Presently biological control and resistant varieties are not widely used. The detection of this pathogen is critical for seed certification and for preventing field infections. In South Korea, bakanae is designated as a regulated pathogen. To provide highly qualified rice seeds to farms, Korea Seed & Variety Service (KSVS) has been producing and distributing certified rice seeds for producing healthy rice in fields. Therefore, the objective of the study is to summarize the recent progress in molecular identification, fungicide resistance, and the management strategy of bakanae.

• ALGAE
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• 제38권4호
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• pp.295-306
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• 2023

Human coronavirus diseases, particularly severe acute respiratory syndrome coronavirus 2, still remain a persistent public health issue, and many recent studies are focusing on the quest for new leads against coronaviruses. To contribute to this growing pool of knowledge and explore the available marine natural products against coronaviruses, this study investigated the antiviral effects of fucoxanthin isolated from Sargassum siliquastrum-a brown alga found on Jeju Island, South Korea. The antiviral effects of fucoxanthin were confirmed in severe acute respiratory syndrome coronavirus 2-infected Vero cells, and its structural characteristics were verified in silico using molecular docking and molecular dynamic simulations and in vitro colorimetric method. Fucoxanthin inhibited the infection in a concentration-dependent manner, without showing cytotoxicity. Molecular docking simulations revealed that fucoxanthin binds to the angiotensinconverting enzyme 2-spike protein (binding energy -318.306 kcal mol^-1) and main protease (binding energy -205.118 kcal mol^-1). Moreover, molecular dynamic simulations showed that fucoxanthin remains docked to angiotensin-converting enzyme 2-spike protein for 20 ns, whereas it breaks away from main protease after 3 ns. Also, the in silico prediction of the fucoxanthin was verified through the in vitro colorimetric method by inhibiting the binding between angiotensinconverting enzyme 2 and spike protein in a concentration-dependent manner. These results indicate that fucoxanthin exhibits antiviral effects against severe acute respiratory syndrome coronavirus 2 by blocking the entry of the virus. Therefore, fucoxanthin from S. siliquastrum can be a potential candidate for treating coronavirus infection.

• Journal of Preventive Medicine and Public Health
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• 제41권1호
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• pp.39-44
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• 2008

Objectives : This study was conducted to investigate the associations of non alcoholic fatty liver with metabolic syndrome and the serum carotenoids. Methods : This study was conducted in a general hospital in South Korea from November, 2004 to August, 2005. The study subjects were 350 sampled persons who were aged from 40 years and older (males : 180, females : 170). They were grouped into the normal, mild and severe groups according to fat accumulation in their livers, as determined by ultrasonography. We analyzed the association between non alcoholic fatty liver and metabolic syndrome by multiple logistic regression analysis and we analyzed the association between non alcoholic fatty liver and the serum carotenoids by a general linear model(ANCOVA). Results : After adjustment for the effect of potential covariates, the prevalence of metabolic syndrome was associated with fat accumulation in the liver (p trend <0.001). If the odds ratio of normal group is 1.00, then that of the mild group is 2.80 (95% C.I=1.17-6.71) and that of the severe group is 7.29 (95% C.I=2.76-19.30). The prevalence of metabolic alterations fitting the criteria of metabolic syndrome, according to the class of fat accumulation in the liver, was significantly increased, except for criteria of high blood pressure, a large waist circumference and low HDL (high density lipoprotein) cholesterol level (p trend <0.001). The level of serum ${\beta}$-carotene was decreased according to the class of fat accumulation in the liver (p trend=0.036), but the levels of serum ${\alpha}$-carotene, lycopene, ${\beta}$-cryptoxanthin and lutein were not decreased. Conclusions : This study shows that non alcoholic fatty liver was associated with metabolic syndrome and with the serum ${\beta}$-carotene level.

• 한국축산식품학회지
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• 제33권6호
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• pp.772-780
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• 2013

지방분해효소 활성이 가장 높은 균은 가을철에 집유한 B 지역의 원유로부터 분리한 Acinetobacter genomospecies 10(match %: 99.90)이었으며, 단백질분해효소 활성이 가장 높은 균은 봄철에 B 지역의 원유로부터 분리한 Serratia liquefaciens(match %: 99.39)였다. Acinetobacter genomospecies 10(match %:99.90)는 전형적인 생장곡선의 형태인 시그모이드 곡선의 형태를 나타냈으며, 배양시간별 조효소 활성은 배양시간이 증가할수록 지방분해효소 활성이 증가하다가 정지기 후반부에서부터 사멸기에 도달하기까지는 배양시간이 길수록 활성이 감소하였다. pH에 따른 지방분해효소 활성은 pH 8.5에서 가장 높았고 온도는 $45^{\circ}C$에서 가장 높은 활성을 나타내었다. Serratia liquefaciens(match %: 99.39)의 생장곡선은 전형적인 시그모이드 형태를 나타내었고, 단백질분해효소 활성은 배양 12시간 후에 가장 높았고, 18시간 후에는 다시 감소하는 양상을 나타냈다. 단백질 분해효소의 pH 영향은 2.5에서 활성이 가장 낮았고 pH 8.5에서 가장 높은 활성을 나타내었다. 또한 반응온도에 의한 효소활성은 $35^{\circ}C$에서 가장 높았다.