• 제목/요약/키워드: Solanum tuberosum

검색결과 236건 처리시간 0.03초

감자(Solanum tuberlosum) 에탄올 추출물의 황산화 활성 (Antioxidant Activity of Ethanolic Extract of Potato(Solanum tuberlosum))

  • 최은미;구성자
    • 동아시아식생활학회지
    • /
    • 제14권5호
    • /
    • pp.479-486
    • /
    • 2004
  • 감자는 전세계적으로 섭취되는 주요 작물로서 우리나라에서는 감자의 생즙을 관절염 및 통증을 억제하는 민간요법으로 사용되어 왔다. 본 연구에서는 감자 에탄을 추출물의 항산화 작용을 검토하였다. 감자 에탄올 추출물(0.05~5 mg/mL) 은 1,1-diphenyl-2-picrylhydrazyl radical에 대하여 강한 free radical scavenger 활성을 나타내었다. 또한 감자 추출물(0.5 mg/mL)은 쥐간 균질액 에서 생성 된 TBARS의 양을 유의적으로 억제하였으며 (p<0.05) Fe/sup 2+//ascorbate induction system에서 감자 추출물(0.5~2 mg/mL)은 DNPH 반응으로 측정된 carbonyl 형성을 억제하였다. 항산화 활성과 마찬가지로 감자 추출물의 환원력은 0.5 mg/mL 농도에서 가장 높았고 이는 α-tocopherol의 경우와 비슷하였다. 이 결과는 감자 추출물이 항산화력을 통해 생체에서 작용할 수 있는 가능성을 제시한다.

  • PDF

An efficient transformation method for a potato (Solanum tuberosum L. var. Atlantic)

  • Han, Eun-Hee;Goo, Young-Min;Lee, Min-Kyung;Lee, Shin-Woo
    • Journal of Plant Biotechnology
    • /
    • 제42권2호
    • /
    • pp.77-82
    • /
    • 2015
  • We found that a long period of in vitro culture is a critical factor on the low transformation rate for a specific potato genotype, Solanum tuberosum L. var. Atlantic when phosphinothricin (PPT) was added to select putative transformants in a solid media. The fresh explants of the newly produced plants from a micro-tuber was able to increase the transformation rate significantly while the old explants prepared from a plant maintained for longer than 6 months in vitro by sub-culturing every 3 ~ 4 weeks resulted in a very low transformation frequency. However, Jowon cultivar was not so much influenced by the period of in vitro culture with high transformation rate (higher than 10.0%). Further research need to be explored for the reason why a particular potato genotype, Atlantic is more vulnerable than the Jowon cultivar during the regeneration stage resulting in the low transformation frequency.

Improved in vitro Regeneration of Potato (Solanum tuberosum cv. Superior) Transformed by Agrobacterium Expressing $\beta-Glucuronidase$

  • Park, Yoon-Kyung;Park, Gene-Sue;Yang, Young-Ki;Cheong, Hyeon-Sook
    • Journal of Plant Biology
    • /
    • 제39권2호
    • /
    • pp.93-98
    • /
    • 1996
  • In order to enhance the system of potato transformation and further regeneration, potato was transformed using the Agrobacterium tumefaciens harboring $\beta$-glucuronidase (GUS) gene. We found that a series fo modified medium ttained 100% shoot regeneration within 5 weeks after the preincubated explants on stage I medium were infected with Agrobacterium. Callus appeared at the cut edges of stem segments on stage II medium, mainly at the basal parts. Some explants started to form shoots after two to three weeks on stage III medium containing kanamycin (50 mg/L). When transferred to MS medium containing 200 mg/L kanamycin, 81% of the transformed shoots formed roots at the cut edge of the plantlets. In contrast, untrasformed shoots never rooted and became yellowish after few weeks under the same conditions. Southern and northern analysis indicated in vitro shoot regeneration on the callus derived from the potato explants, which were incubated with Agrobacteria. The regeneration cycle was shortened after the transformatin and finally the transformation efficiency was highly enhanced.

  • PDF

The Potato Transcriptional Co-activator StMBF1 Is Up-regulated in Response to Oxidative Stress and Interacts with the TATA-box Binding Protein

  • Arce, Debora Pamela;Tonon, Claudia;Zanetti, Maria Eugenia;Godoy, Andrea Veronica;Hirose, Susumu;Casalongue, Claudia Anahi
    • BMB Reports
    • /
    • 제39권4호
    • /
    • pp.355-360
    • /
    • 2006
  • To gain a better understanding on the function of the potato Solanum tuberosum Multiprotein Bridging Factor 1 protein (StMBF1) its interaction with the TATA box binding protein (TBP) was demonstrated. In addition we reported that StMBF1 rescues the yeast mbf1 mutant phenotype, indicating its role as a plant co-activator. These data reinforce the hypothesis that MBF1 function is also conserved among non closely related plant species. In addition, measurement of StMBF1 protein level by Western blot using anti-StMBF1 antibodies indicated that the protein level increased upon $H_2O_2$ and heat shock treatments. However, the potato $\beta$-1,3-glucanase protein level was not changed under the same experimental conditions. These data indicate that StMBF1 participates in the cell stress response against oxidative stress allowing us to suggest that MBF1 genes from different plant groups may share similar functions.

Inhibition of Experimental Gastric Ulcer by Potato Tubers and the Starch

  • Lee, Jun-Gi;Jin, Jeong-Ho;Lim, Hak-Tae;Choi, Hee-Don;Kim, Hyun-Pyo
    • Natural Product Sciences
    • /
    • 제15권3호
    • /
    • pp.134-138
    • /
    • 2009
  • In an attempt to establish anti-ulcerogenic activity of potato tubers, inhibitory activity against ethanol- and indomethacin-induced gastric ulcer models in rats was evaluated for the first time. From several varieties of potato tubers including Solanum tuberosum L. cv. Superior (white skin and fresh potato) and two new varieties of (Bora valley and Gogu valley), raw potato juice was prepared and the starch was obtained from each juice by filtration and drying. Upon oral administration to rats, raw potato juice showed more or less inhibitory activity. The starch showed higher and dose-dependent inhibitory activity, suggesting that the active ingredient in raw potato juice may be the starch. Particularly, the starch obtained from the tubers of new potato variety, "Bora valley," with purple color, showed the highest inhibitory activity (62.4% and 37.1% inhibition of ulcer index at 500 mg/kg), while omeprazole (proton pump inhibitor) used as a reference drug showed 74.4% and 75.7% inhibition at 20 mg/kg against ethanol- and indomethacin-induced ulcer formation, respectively. The present study provides a first evidence of anti-ulcerogenicity of raw potato juice and the starch. Especially, the starch from "Bora valley" strongly inhibited ulcer formation in rats. Considering that these are food components, they may be safely used for anti-ulcerogenic nutraceuticals.

Effect of Root Zone Cooling on Growth Responses and Tuberization of Hydroponically Grown 'Superior' Potato (Solanum tuberosum) in Summer

  • Chang, Dong-Chil;Jeong, Jin-Cheol;Lee, Yong-Beom
    • 생물환경조절학회지
    • /
    • 제15권4호
    • /
    • pp.340-345
    • /
    • 2006
  • A potato (Solanum tuberosum L. cv. Superior) cultivar was grown in aeroponic cultivation system to investigate the effect of root zone cooling in summer. Based on their nutrient uptake, growth responses, and tuberization, the possibilities for potato seed production were determined. Although shoot growth and early tuberization increased in the conventional non-cooling root zone system (root zone temperature of $25\pm2^{\circ}C$), stolen growth, photosynthesis, transpiration rate and number of tubers produced were higher in the cooling root zone system ($20\pm2^{\circ}C$) than in the non-cooling system. Increasing root zone temperature above $25^{\circ}C$ stimulated absorption of K more than T-N, P, Ca, Fe and Mn. On the other hand, root zone temperatures in the range of $20^{\circ}C$ to $25^{\circ}C$ did not affect Mg contents. The lower uptake and supply to leaves of T-N, Fe and Mn at the high root zone temperature promoted early tuberization and advanced haulm senescence. The results stress the importance of keeping root zone temperature to as low as below 20, particularly in summer under temperate Bone.